Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0436 and Shew_0437 are separated by 133 nucleotides Shew_0437 and Shew_0438 are separated by 48 nucleotides Shew_0438 and Shew_0439 are separated by 107 nucleotides Shew_0439 and Shew_0440 are separated by 8 nucleotides
Shew_0436: Shew_0436 - diguanylate cyclase/phosphodiesterase (RefSeq), at 486,271 to 488,043
_0436
Shew_0437: Shew_0437 - hypothetical protein (RefSeq), at 488,177 to 488,488
_0437
Shew_0438: Shew_0438 - GumN family protein (RefSeq), at 488,537 to 489,421
_0438
Shew_0439: Shew_0439 - CheD, stimulates methylation of MCP proteins (RefSeq), at 489,529 to 490,161
_0439
Shew_0440: Shew_0440 - methyltransferase type 12 (RefSeq), at 490,170 to 491,024
_0440
Position (kb) 488
489
490 Strain fitness (log2 ratio) -1
0
1 at 487.897 kb on - strand at 487.929 kb on - strand at 488.023 kb on + strand at 488.086 kb on + strand at 488.094 kb on - strand at 489.092 kb on - strand, within Shew_0438 at 489.658 kb on + strand, within Shew_0439 at 489.916 kb on - strand, within Shew_0439 at 490.044 kb on + strand, within Shew_0439 at 490.214 kb on - strand at 490.405 kb on - strand, within Shew_0440
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 487,897 - +0.2 487,929 - +0.1 488,023 + +1.0 488,086 + +0.2 488,094 - +0.0 489,092 - Shew_0438 0.63 -0.5 489,658 + Shew_0439 0.20 -0.1 489,916 - Shew_0439 0.61 +0.1 490,044 + Shew_0439 0.81 +0.4 490,214 - -0.2 490,405 - Shew_0440 0.27 -0.2
Or see this region's nucleotide sequence