Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapF and Shew_0324 are separated by 8 nucleotides Shew_0324 and Shew_0325 are separated by 7 nucleotides Shew_0325 and Shew_0326 overlap by 8 nucleotides Shew_0326 and Shew_0327 are separated by 101 nucleotides
Shew_0323: dapF - diaminopimelate epimerase (RefSeq), at 369,549 to 370,388
dapF
Shew_0324: Shew_0324 - hypothetical protein (RefSeq), at 370,397 to 371,023
_0324
Shew_0325: Shew_0325 - tyrosine recombinase XerC (RefSeq), at 371,031 to 371,924
_0325
Shew_0326: Shew_0326 - HAD family hydrolase (RefSeq), at 371,917 to 372,639
_0326
Shew_0327: Shew_0327 - HupE/UreJ protein (RefSeq), at 372,741 to 373,316
_0327
Position (kb) 371
372 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 370.489 kb on + strand, within Shew_0324 at 370.533 kb on + strand, within Shew_0324 at 370.666 kb on + strand, within Shew_0324 at 371.311 kb on + strand, within Shew_0325 at 371.516 kb on + strand, within Shew_0325 at 371.664 kb on + strand, within Shew_0325 at 371.742 kb on - strand, within Shew_0325 at 371.805 kb on + strand, within Shew_0325 at 371.837 kb on + strand at 371.837 kb on + strand at 371.845 kb on - strand at 371.874 kb on + strand at 372.400 kb on + strand, within Shew_0326 at 372.413 kb on + strand, within Shew_0326 at 372.474 kb on - strand, within Shew_0326 at 372.750 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 370,489 + Shew_0324 0.15 -0.6 370,533 + Shew_0324 0.22 +0.1 370,666 + Shew_0324 0.43 -1.9 371,311 + Shew_0325 0.31 -1.2 371,516 + Shew_0325 0.54 -0.8 371,664 + Shew_0325 0.71 -0.9 371,742 - Shew_0325 0.80 +2.1 371,805 + Shew_0325 0.87 -0.9 371,837 + +0.6 371,837 + -1.6 371,845 - +0.1 371,874 + -3.2 372,400 + Shew_0326 0.67 -0.4 372,413 + Shew_0326 0.69 -0.7 372,474 - Shew_0326 0.77 -0.8 372,750 + +0.9
Or see this region's nucleotide sequence