Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0214 and Shew_0215 are separated by 16 nucleotides Shew_0215 and Shew_0216 are separated by 52 nucleotides Shew_0216 and Shew_0217 are separated by 74 nucleotides
Shew_0214: Shew_0214 - 3-dehydroquinate synthase (RefSeq), at 229,315 to 230,391
_0214
Shew_0215: Shew_0215 - sporulation domain-containing protein (RefSeq), at 230,408 to 231,943
_0215
Shew_0216: Shew_0216 - DNA adenine methylase (RefSeq), at 231,996 to 232,835
_0216
Shew_0217: Shew_0217 - hypothetical protein (RefSeq), at 232,910 to 233,083
_0217
Position (kb) 230
231
232 Strain fitness (log2 ratio) -2
-1
0
1
2 at 229.593 kb on - strand, within Shew_0214 at 229.767 kb on + strand, within Shew_0214 at 229.775 kb on - strand, within Shew_0214 at 229.827 kb on - strand, within Shew_0214 at 229.915 kb on - strand, within Shew_0214 at 229.991 kb on - strand, within Shew_0214 at 230.060 kb on - strand, within Shew_0214 at 230.522 kb on + strand at 230.579 kb on - strand, within Shew_0215 at 230.721 kb on + strand, within Shew_0215 at 230.769 kb on - strand, within Shew_0215 at 230.791 kb on + strand, within Shew_0215 at 230.937 kb on - strand, within Shew_0215 at 231.046 kb on - strand, within Shew_0215 at 231.287 kb on - strand, within Shew_0215 at 231.450 kb on + strand, within Shew_0215 at 231.463 kb on + strand, within Shew_0215 at 231.577 kb on + strand, within Shew_0215 at 231.580 kb on - strand, within Shew_0215 at 231.945 kb on + strand at 232.439 kb on + strand, within Shew_0216 at 232.497 kb on - strand, within Shew_0216 at 232.877 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 229,593 - Shew_0214 0.26 +1.9 229,767 + Shew_0214 0.42 +0.7 229,775 - Shew_0214 0.43 +0.2 229,827 - Shew_0214 0.48 -1.7 229,915 - Shew_0214 0.56 +1.5 229,991 - Shew_0214 0.63 -0.1 230,060 - Shew_0214 0.69 -2.0 230,522 + +0.5 230,579 - Shew_0215 0.11 +0.4 230,721 + Shew_0215 0.20 +0.3 230,769 - Shew_0215 0.24 +0.3 230,791 + Shew_0215 0.25 +0.6 230,937 - Shew_0215 0.34 +0.8 231,046 - Shew_0215 0.42 +0.2 231,287 - Shew_0215 0.57 +0.2 231,450 + Shew_0215 0.68 +0.1 231,463 + Shew_0215 0.69 -0.1 231,577 + Shew_0215 0.76 +1.1 231,580 - Shew_0215 0.76 +1.2 231,945 + -0.2 232,439 + Shew_0216 0.53 +0.6 232,497 - Shew_0216 0.60 -1.8 232,877 + -1.1
Or see this region's nucleotide sequence