Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4474

Experiment: perchlorate 50 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4473 and Pf6N2E2_4474 are separated by 81 nucleotidesPf6N2E2_4474 and Pf6N2E2_4475 are separated by 194 nucleotidesPf6N2E2_4475 and Pf6N2E2_4476 are separated by 35 nucleotides Pf6N2E2_4473 - HlyD family secretion protein, at 5,207,268 to 5,208,233 _4473 Pf6N2E2_4474 - FIG006231: RNA-binding protein, at 5,208,315 to 5,208,764 _4474 Pf6N2E2_4475 - FIG00953390: hypothetical protein, at 5,208,959 to 5,209,105 _4475 Pf6N2E2_4476 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 5,209,141 to 5,209,746 _4476 Position (kb) 5208 5209Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5207.370 kb on + strand, within Pf6N2E2_4473at 5207.370 kb on + strand, within Pf6N2E2_4473at 5207.371 kb on - strand, within Pf6N2E2_4473at 5207.371 kb on - strand, within Pf6N2E2_4473at 5207.371 kb on - strand, within Pf6N2E2_4473at 5207.551 kb on + strand, within Pf6N2E2_4473at 5207.551 kb on + strand, within Pf6N2E2_4473at 5207.551 kb on + strand, within Pf6N2E2_4473at 5207.551 kb on + strand, within Pf6N2E2_4473at 5207.563 kb on + strand, within Pf6N2E2_4473at 5207.563 kb on + strand, within Pf6N2E2_4473at 5207.563 kb on + strand, within Pf6N2E2_4473at 5207.563 kb on + strand, within Pf6N2E2_4473at 5207.563 kb on + strand, within Pf6N2E2_4473at 5207.563 kb on + strand, within Pf6N2E2_4473at 5207.563 kb on + strand, within Pf6N2E2_4473at 5207.564 kb on - strand, within Pf6N2E2_4473at 5207.564 kb on - strand, within Pf6N2E2_4473at 5207.564 kb on - strand, within Pf6N2E2_4473at 5207.564 kb on - strand, within Pf6N2E2_4473at 5207.564 kb on - strand, within Pf6N2E2_4473at 5207.564 kb on - strand, within Pf6N2E2_4473at 5207.564 kb on - strand, within Pf6N2E2_4473at 5207.564 kb on - strand, within Pf6N2E2_4473at 5207.682 kb on + strand, within Pf6N2E2_4473at 5207.682 kb on + strand, within Pf6N2E2_4473at 5207.682 kb on + strand, within Pf6N2E2_4473at 5207.683 kb on - strand, within Pf6N2E2_4473at 5207.683 kb on - strand, within Pf6N2E2_4473at 5207.683 kb on - strand, within Pf6N2E2_4473at 5207.683 kb on - strand, within Pf6N2E2_4473at 5207.683 kb on - strand, within Pf6N2E2_4473at 5207.683 kb on - strand, within Pf6N2E2_4473at 5207.746 kb on - strand, within Pf6N2E2_4473at 5207.828 kb on - strand, within Pf6N2E2_4473at 5208.215 kb on + strandat 5208.215 kb on + strandat 5208.215 kb on + strandat 5208.215 kb on + strandat 5208.215 kb on + strandat 5208.215 kb on + strandat 5208.216 kb on - strandat 5208.216 kb on - strandat 5208.252 kb on - strandat 5208.253 kb on + strandat 5208.253 kb on + strandat 5208.253 kb on + strandat 5208.254 kb on - strandat 5208.254 kb on - strandat 5208.353 kb on + strandat 5208.427 kb on + strand, within Pf6N2E2_4474at 5208.428 kb on - strand, within Pf6N2E2_4474at 5208.428 kb on - strand, within Pf6N2E2_4474at 5208.520 kb on + strand, within Pf6N2E2_4474at 5208.520 kb on + strand, within Pf6N2E2_4474at 5208.520 kb on + strand, within Pf6N2E2_4474at 5208.520 kb on + strand, within Pf6N2E2_4474at 5208.520 kb on + strand, within Pf6N2E2_4474at 5208.520 kb on + strand, within Pf6N2E2_4474at 5208.520 kb on + strand, within Pf6N2E2_4474at 5208.520 kb on + strand, within Pf6N2E2_4474at 5208.521 kb on - strand, within Pf6N2E2_4474at 5208.521 kb on - strand, within Pf6N2E2_4474at 5208.521 kb on - strand, within Pf6N2E2_4474at 5208.521 kb on - strand, within Pf6N2E2_4474at 5208.521 kb on - strand, within Pf6N2E2_4474at 5208.521 kb on - strand, within Pf6N2E2_4474at 5208.521 kb on - strand, within Pf6N2E2_4474at 5208.616 kb on + strand, within Pf6N2E2_4474at 5208.616 kb on + strand, within Pf6N2E2_4474at 5208.617 kb on - strand, within Pf6N2E2_4474at 5208.670 kb on + strand, within Pf6N2E2_4474at 5208.670 kb on + strand, within Pf6N2E2_4474at 5208.670 kb on + strand, within Pf6N2E2_4474at 5208.670 kb on + strand, within Pf6N2E2_4474at 5208.671 kb on - strand, within Pf6N2E2_4474at 5208.671 kb on - strand, within Pf6N2E2_4474at 5208.726 kb on + strandat 5208.757 kb on + strandat 5208.758 kb on - strandat 5208.771 kb on + strandat 5208.772 kb on - strandat 5208.810 kb on - strandat 5208.907 kb on + strandat 5208.937 kb on + strandat 5208.938 kb on - strandat 5208.938 kb on - strandat 5208.938 kb on - strandat 5209.153 kb on - strandat 5209.154 kb on + strandat 5209.154 kb on + strandat 5209.154 kb on + strandat 5209.154 kb on + strandat 5209.154 kb on + strandat 5209.154 kb on + strandat 5209.154 kb on + strandat 5209.154 kb on + strandat 5209.155 kb on - strandat 5209.155 kb on - strandat 5209.155 kb on - strandat 5209.155 kb on - strandat 5209.155 kb on - strandat 5209.155 kb on - strandat 5209.189 kb on + strandat 5209.189 kb on + strandat 5209.190 kb on - strandat 5209.190 kb on - strandat 5209.287 kb on - strand, within Pf6N2E2_4476at 5209.304 kb on + strand, within Pf6N2E2_4476at 5209.305 kb on - strand, within Pf6N2E2_4476at 5209.514 kb on + strand, within Pf6N2E2_4476at 5209.514 kb on + strand, within Pf6N2E2_4476at 5209.515 kb on - strand, within Pf6N2E2_4476at 5209.515 kb on - strand, within Pf6N2E2_4476at 5209.515 kb on - strand, within Pf6N2E2_4476at 5209.515 kb on - strand, within Pf6N2E2_4476at 5209.515 kb on - strand, within Pf6N2E2_4476at 5209.515 kb on - strand, within Pf6N2E2_4476at 5209.515 kb on - strand, within Pf6N2E2_4476at 5209.515 kb on - strand, within Pf6N2E2_4476at 5209.515 kb on - strand, within Pf6N2E2_4476at 5209.586 kb on + strand, within Pf6N2E2_4476at 5209.746 kb on + strandat 5209.746 kb on + strandat 5209.746 kb on + strandat 5209.746 kb on + strandat 5209.747 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction perchlorate 50 mM
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5,207,370 + Pf6N2E2_4473 0.11 +0.5
5,207,370 + Pf6N2E2_4473 0.11 +0.3
5,207,371 - Pf6N2E2_4473 0.11 -0.1
5,207,371 - Pf6N2E2_4473 0.11 -0.1
5,207,371 - Pf6N2E2_4473 0.11 -0.2
5,207,551 + Pf6N2E2_4473 0.29 +0.6
5,207,551 + Pf6N2E2_4473 0.29 -0.9
5,207,551 + Pf6N2E2_4473 0.29 +1.4
5,207,551 + Pf6N2E2_4473 0.29 -0.1
5,207,563 + Pf6N2E2_4473 0.31 +0.4
5,207,563 + Pf6N2E2_4473 0.31 -0.6
5,207,563 + Pf6N2E2_4473 0.31 +0.4
5,207,563 + Pf6N2E2_4473 0.31 +0.5
5,207,563 + Pf6N2E2_4473 0.31 -1.0
5,207,563 + Pf6N2E2_4473 0.31 -0.4
5,207,563 + Pf6N2E2_4473 0.31 -0.0
5,207,564 - Pf6N2E2_4473 0.31 -0.3
5,207,564 - Pf6N2E2_4473 0.31 -0.3
5,207,564 - Pf6N2E2_4473 0.31 +0.5
5,207,564 - Pf6N2E2_4473 0.31 +0.0
5,207,564 - Pf6N2E2_4473 0.31 -0.2
5,207,564 - Pf6N2E2_4473 0.31 -0.4
5,207,564 - Pf6N2E2_4473 0.31 -0.1
5,207,564 - Pf6N2E2_4473 0.31 +0.6
5,207,682 + Pf6N2E2_4473 0.43 +0.4
5,207,682 + Pf6N2E2_4473 0.43 -0.7
5,207,682 + Pf6N2E2_4473 0.43 +0.3
5,207,683 - Pf6N2E2_4473 0.43 -0.4
5,207,683 - Pf6N2E2_4473 0.43 +0.0
5,207,683 - Pf6N2E2_4473 0.43 -1.3
5,207,683 - Pf6N2E2_4473 0.43 -1.4
5,207,683 - Pf6N2E2_4473 0.43 +0.2
5,207,683 - Pf6N2E2_4473 0.43 -0.2
5,207,746 - Pf6N2E2_4473 0.49 -0.1
5,207,828 - Pf6N2E2_4473 0.58 +0.1
5,208,215 + +0.4
5,208,215 + -0.1
5,208,215 + -0.1
5,208,215 + +0.0
5,208,215 + -0.3
5,208,215 + -0.8
5,208,216 - +0.2
5,208,216 - +0.4
5,208,252 - -0.3
5,208,253 + -1.1
5,208,253 + +0.6
5,208,253 + +0.0
5,208,254 - -0.3
5,208,254 - -0.6
5,208,353 + -1.2
5,208,427 + Pf6N2E2_4474 0.25 -0.0
5,208,428 - Pf6N2E2_4474 0.25 -1.5
5,208,428 - Pf6N2E2_4474 0.25 -1.5
5,208,520 + Pf6N2E2_4474 0.46 -0.5
5,208,520 + Pf6N2E2_4474 0.46 -0.4
5,208,520 + Pf6N2E2_4474 0.46 -0.5
5,208,520 + Pf6N2E2_4474 0.46 -1.6
5,208,520 + Pf6N2E2_4474 0.46 +0.1
5,208,520 + Pf6N2E2_4474 0.46 -1.4
5,208,520 + Pf6N2E2_4474 0.46 -0.8
5,208,520 + Pf6N2E2_4474 0.46 -0.8
5,208,521 - Pf6N2E2_4474 0.46 +0.8
5,208,521 - Pf6N2E2_4474 0.46 -1.0
5,208,521 - Pf6N2E2_4474 0.46 -0.5
5,208,521 - Pf6N2E2_4474 0.46 -1.4
5,208,521 - Pf6N2E2_4474 0.46 -1.3
5,208,521 - Pf6N2E2_4474 0.46 -0.8
5,208,521 - Pf6N2E2_4474 0.46 -0.4
5,208,616 + Pf6N2E2_4474 0.67 +0.3
5,208,616 + Pf6N2E2_4474 0.67 +0.7
5,208,617 - Pf6N2E2_4474 0.67 -0.8
5,208,670 + Pf6N2E2_4474 0.79 +0.3
5,208,670 + Pf6N2E2_4474 0.79 -1.5
5,208,670 + Pf6N2E2_4474 0.79 -2.3
5,208,670 + Pf6N2E2_4474 0.79 -0.1
5,208,671 - Pf6N2E2_4474 0.79 +0.2
5,208,671 - Pf6N2E2_4474 0.79 -1.3
5,208,726 + -1.4
5,208,757 + -0.5
5,208,758 - +0.3
5,208,771 + +0.5
5,208,772 - +3.1
5,208,810 - +0.3
5,208,907 + +0.5
5,208,937 + +1.0
5,208,938 - -0.7
5,208,938 - -1.1
5,208,938 - -1.3
5,209,153 - +0.3
5,209,154 + -0.3
5,209,154 + +0.1
5,209,154 + -0.1
5,209,154 + +0.2
5,209,154 + -0.2
5,209,154 + -1.1
5,209,154 + +0.1
5,209,154 + +0.1
5,209,155 - +0.0
5,209,155 - -0.3
5,209,155 - -0.6
5,209,155 - -0.1
5,209,155 - -0.5
5,209,155 - -0.1
5,209,189 + -1.0
5,209,189 + -0.5
5,209,190 - +0.0
5,209,190 - +0.3
5,209,287 - Pf6N2E2_4476 0.24 -0.5
5,209,304 + Pf6N2E2_4476 0.27 -1.4
5,209,305 - Pf6N2E2_4476 0.27 +0.6
5,209,514 + Pf6N2E2_4476 0.62 -0.6
5,209,514 + Pf6N2E2_4476 0.62 +0.0
5,209,515 - Pf6N2E2_4476 0.62 -1.6
5,209,515 - Pf6N2E2_4476 0.62 +0.2
5,209,515 - Pf6N2E2_4476 0.62 -0.4
5,209,515 - Pf6N2E2_4476 0.62 -0.2
5,209,515 - Pf6N2E2_4476 0.62 -0.2
5,209,515 - Pf6N2E2_4476 0.62 -0.3
5,209,515 - Pf6N2E2_4476 0.62 -1.1
5,209,515 - Pf6N2E2_4476 0.62 -0.2
5,209,515 - Pf6N2E2_4476 0.62 -0.3
5,209,586 + Pf6N2E2_4476 0.73 -0.7
5,209,746 + -2.1
5,209,746 + +0.3
5,209,746 + +0.4
5,209,746 + +0.2
5,209,747 - -0.2

Or see this region's nucleotide sequence