Experiment: perchlorate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3532 and Pf6N2E2_3533 overlap by 4 nucleotides Pf6N2E2_3533 and Pf6N2E2_3534 overlap by 8 nucleotides Pf6N2E2_3534 and Pf6N2E2_3535 are separated by 390 nucleotides
Pf6N2E2_3532 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 4,122,713 to 4,123,447
_3532
Pf6N2E2_3533 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 4,123,444 to 4,124,070
_3533
Pf6N2E2_3534 - Cobalamin biosynthesis protein CobG, at 4,124,063 to 4,125,331
_3534
Pf6N2E2_3535 - Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-), at 4,125,722 to 4,126,933
_3535
Position (kb)
4124
4125
4126 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4123.063 kb on - strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.090 kb on - strand, within Pf6N2E2_3532 at 4123.090 kb on - strand, within Pf6N2E2_3532 at 4123.090 kb on - strand, within Pf6N2E2_3532 at 4123.095 kb on + strand, within Pf6N2E2_3532 at 4123.095 kb on + strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.212 kb on + strand, within Pf6N2E2_3532 at 4123.213 kb on - strand, within Pf6N2E2_3532 at 4123.252 kb on - strand, within Pf6N2E2_3532 at 4123.339 kb on - strand, within Pf6N2E2_3532 at 4123.339 kb on - strand, within Pf6N2E2_3532 at 4123.646 kb on + strand, within Pf6N2E2_3533 at 4123.646 kb on + strand, within Pf6N2E2_3533 at 4123.646 kb on + strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.061 kb on - strand at 4124.061 kb on - strand at 4124.061 kb on - strand at 4124.061 kb on - strand at 4124.119 kb on + strand at 4124.119 kb on + strand at 4124.119 kb on + strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.123 kb on + strand at 4124.123 kb on + strand at 4124.124 kb on - strand at 4124.124 kb on - strand at 4124.124 kb on - strand at 4124.175 kb on + strand at 4124.175 kb on + strand at 4124.494 kb on + strand, within Pf6N2E2_3534 at 4124.494 kb on + strand, within Pf6N2E2_3534 at 4124.495 kb on - strand, within Pf6N2E2_3534 at 4124.495 kb on - strand, within Pf6N2E2_3534 at 4125.035 kb on - strand, within Pf6N2E2_3534 at 4125.353 kb on + strand at 4125.353 kb on + strand at 4125.354 kb on - strand at 4125.354 kb on - strand at 4125.395 kb on - strand at 4125.430 kb on + strand at 4125.430 kb on + strand at 4125.431 kb on - strand at 4125.506 kb on + strand at 4125.506 kb on + strand at 4125.506 kb on + strand at 4125.506 kb on + strand at 4125.506 kb on + strand at 4125.506 kb on + strand at 4125.507 kb on - strand at 4125.507 kb on - strand at 4125.638 kb on + strand at 4125.639 kb on - strand at 4125.639 kb on - strand at 4125.639 kb on - strand at 4125.682 kb on - strand at 4125.741 kb on + strand at 4125.962 kb on + strand, within Pf6N2E2_3535 at 4125.962 kb on + strand, within Pf6N2E2_3535 at 4125.962 kb on + strand, within Pf6N2E2_3535 at 4125.963 kb on - strand, within Pf6N2E2_3535 at 4125.963 kb on - strand, within Pf6N2E2_3535 at 4125.963 kb on - strand, within Pf6N2E2_3535 at 4125.963 kb on - strand, within Pf6N2E2_3535 at 4125.963 kb on - strand, within Pf6N2E2_3535 at 4126.128 kb on + strand, within Pf6N2E2_3535 at 4126.128 kb on + strand, within Pf6N2E2_3535 at 4126.128 kb on + strand, within Pf6N2E2_3535
Per-strain Table
Position Strand Gene LocusTag Fraction perchlorate 50 mM remove 4,123,063 - Pf6N2E2_3532 0.48 -0.8 4,123,089 + Pf6N2E2_3532 0.51 +0.1 4,123,089 + Pf6N2E2_3532 0.51 -0.1 4,123,089 + Pf6N2E2_3532 0.51 -0.1 4,123,089 + Pf6N2E2_3532 0.51 +0.7 4,123,089 + Pf6N2E2_3532 0.51 +0.2 4,123,089 + Pf6N2E2_3532 0.51 -0.8 4,123,089 + Pf6N2E2_3532 0.51 +0.4 4,123,090 - Pf6N2E2_3532 0.51 +0.2 4,123,090 - Pf6N2E2_3532 0.51 -0.8 4,123,090 - Pf6N2E2_3532 0.51 -0.5 4,123,095 + Pf6N2E2_3532 0.52 -1.0 4,123,095 + Pf6N2E2_3532 0.52 -0.3 4,123,096 - Pf6N2E2_3532 0.52 -1.1 4,123,096 - Pf6N2E2_3532 0.52 -0.7 4,123,096 - Pf6N2E2_3532 0.52 -0.1 4,123,096 - Pf6N2E2_3532 0.52 -0.3 4,123,096 - Pf6N2E2_3532 0.52 +0.2 4,123,096 - Pf6N2E2_3532 0.52 -0.9 4,123,096 - Pf6N2E2_3532 0.52 -0.5 4,123,212 + Pf6N2E2_3532 0.68 -0.6 4,123,213 - Pf6N2E2_3532 0.68 +0.3 4,123,252 - Pf6N2E2_3532 0.73 +0.2 4,123,339 - Pf6N2E2_3532 0.85 -0.6 4,123,339 - Pf6N2E2_3532 0.85 -0.5 4,123,646 + Pf6N2E2_3533 0.32 -0.3 4,123,646 + Pf6N2E2_3533 0.32 -0.1 4,123,646 + Pf6N2E2_3533 0.32 -1.8 4,123,647 - Pf6N2E2_3533 0.32 -0.0 4,123,647 - Pf6N2E2_3533 0.32 -0.7 4,123,647 - Pf6N2E2_3533 0.32 -1.4 4,123,647 - Pf6N2E2_3533 0.32 -1.4 4,123,647 - Pf6N2E2_3533 0.32 -0.0 4,124,060 + -0.2 4,124,060 + -1.4 4,124,060 + -0.1 4,124,060 + -0.4 4,124,060 + +0.0 4,124,060 + -0.4 4,124,060 + -0.1 4,124,061 - +0.1 4,124,061 - -1.1 4,124,061 - +0.8 4,124,061 - -0.9 4,124,119 + -1.1 4,124,119 + -0.4 4,124,119 + -0.6 4,124,120 - -0.4 4,124,120 - -0.4 4,124,120 - +0.2 4,124,120 - +0.5 4,124,120 - -0.8 4,124,123 + +0.1 4,124,123 + -0.4 4,124,124 - +0.7 4,124,124 - -0.5 4,124,124 - -0.5 4,124,175 + -0.0 4,124,175 + -0.9 4,124,494 + Pf6N2E2_3534 0.34 -1.7 4,124,494 + Pf6N2E2_3534 0.34 -3.1 4,124,495 - Pf6N2E2_3534 0.34 -0.6 4,124,495 - Pf6N2E2_3534 0.34 +0.1 4,125,035 - Pf6N2E2_3534 0.77 +0.1 4,125,353 + -0.0 4,125,353 + -0.5 4,125,354 - -0.1 4,125,354 - +0.6 4,125,395 - -0.6 4,125,430 + +0.2 4,125,430 + +0.4 4,125,431 - -0.2 4,125,506 + +0.1 4,125,506 + -0.2 4,125,506 + -0.2 4,125,506 + -0.2 4,125,506 + -0.9 4,125,506 + -0.6 4,125,507 - -0.8 4,125,507 - -0.5 4,125,638 + +0.3 4,125,639 - +0.0 4,125,639 - +0.3 4,125,639 - -0.2 4,125,682 - +0.1 4,125,741 + -0.1 4,125,962 + Pf6N2E2_3535 0.20 +0.3 4,125,962 + Pf6N2E2_3535 0.20 -0.5 4,125,962 + Pf6N2E2_3535 0.20 -0.4 4,125,963 - Pf6N2E2_3535 0.20 +1.5 4,125,963 - Pf6N2E2_3535 0.20 -0.1 4,125,963 - Pf6N2E2_3535 0.20 +0.3 4,125,963 - Pf6N2E2_3535 0.20 -1.0 4,125,963 - Pf6N2E2_3535 0.20 -0.9 4,126,128 + Pf6N2E2_3535 0.33 +0.4 4,126,128 + Pf6N2E2_3535 0.33 -0.1 4,126,128 + Pf6N2E2_3535 0.33 +0.1
Or see this region's nucleotide sequence