Experiment: perchlorate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_318 and Pf6N2E2_319 are separated by 206 nucleotides Pf6N2E2_319 and Pf6N2E2_320 overlap by 4 nucleotides
Pf6N2E2_318 - Gluconolactonase (EC 3.1.1.17), at 385,447 to 386,538
_318
Pf6N2E2_319 - DUF1850 domain-containing protein, at 386,745 to 387,131
_319
Pf6N2E2_320 - TRAP transporter, 4TM/12TM fusion protein, unknown substrate 1, at 387,128 to 389,155
_320
Position (kb)
386
387
388 Strain fitness (log2 ratio)
-2
-1
0
1 at 385.911 kb on + strand, within Pf6N2E2_318 at 386.030 kb on - strand, within Pf6N2E2_318 at 386.071 kb on + strand, within Pf6N2E2_318 at 386.167 kb on + strand, within Pf6N2E2_318 at 386.167 kb on + strand, within Pf6N2E2_318 at 386.168 kb on - strand, within Pf6N2E2_318 at 386.497 kb on + strand at 386.497 kb on + strand at 386.497 kb on + strand at 386.498 kb on - strand at 386.498 kb on - strand at 386.498 kb on - strand at 386.605 kb on - strand at 386.614 kb on - strand at 386.841 kb on + strand, within Pf6N2E2_319 at 386.842 kb on - strand, within Pf6N2E2_319 at 386.842 kb on - strand, within Pf6N2E2_319 at 386.912 kb on - strand, within Pf6N2E2_319 at 387.351 kb on + strand, within Pf6N2E2_320 at 387.352 kb on - strand, within Pf6N2E2_320 at 387.400 kb on - strand, within Pf6N2E2_320 at 387.400 kb on - strand, within Pf6N2E2_320 at 387.400 kb on - strand, within Pf6N2E2_320 at 387.546 kb on + strand, within Pf6N2E2_320 at 387.546 kb on + strand, within Pf6N2E2_320 at 387.627 kb on + strand, within Pf6N2E2_320 at 387.627 kb on + strand, within Pf6N2E2_320 at 387.627 kb on + strand, within Pf6N2E2_320 at 387.628 kb on - strand, within Pf6N2E2_320 at 387.646 kb on + strand, within Pf6N2E2_320 at 387.726 kb on + strand, within Pf6N2E2_320 at 387.830 kb on + strand, within Pf6N2E2_320 at 387.830 kb on + strand, within Pf6N2E2_320 at 387.831 kb on - strand, within Pf6N2E2_320 at 387.993 kb on + strand, within Pf6N2E2_320 at 387.994 kb on - strand, within Pf6N2E2_320 at 388.099 kb on - strand, within Pf6N2E2_320 at 388.099 kb on - strand, within Pf6N2E2_320
Per-strain Table
Position Strand Gene LocusTag Fraction perchlorate 50 mM remove 385,911 + Pf6N2E2_318 0.42 -0.9 386,030 - Pf6N2E2_318 0.53 -0.3 386,071 + Pf6N2E2_318 0.57 -0.2 386,167 + Pf6N2E2_318 0.66 -0.1 386,167 + Pf6N2E2_318 0.66 +0.2 386,168 - Pf6N2E2_318 0.66 -0.0 386,497 + -0.4 386,497 + +0.4 386,497 + +0.1 386,498 - -1.0 386,498 - -1.6 386,498 - +0.3 386,605 - -1.0 386,614 - -0.5 386,841 + Pf6N2E2_319 0.25 -0.5 386,842 - Pf6N2E2_319 0.25 +0.4 386,842 - Pf6N2E2_319 0.25 -1.2 386,912 - Pf6N2E2_319 0.43 -1.0 387,351 + Pf6N2E2_320 0.11 +0.2 387,352 - Pf6N2E2_320 0.11 +0.0 387,400 - Pf6N2E2_320 0.13 -0.1 387,400 - Pf6N2E2_320 0.13 +0.1 387,400 - Pf6N2E2_320 0.13 -0.4 387,546 + Pf6N2E2_320 0.21 -0.3 387,546 + Pf6N2E2_320 0.21 -0.3 387,627 + Pf6N2E2_320 0.25 -1.8 387,627 + Pf6N2E2_320 0.25 -0.1 387,627 + Pf6N2E2_320 0.25 -0.6 387,628 - Pf6N2E2_320 0.25 -1.7 387,646 + Pf6N2E2_320 0.26 +0.0 387,726 + Pf6N2E2_320 0.29 -0.1 387,830 + Pf6N2E2_320 0.35 -0.1 387,830 + Pf6N2E2_320 0.35 +0.9 387,831 - Pf6N2E2_320 0.35 -0.3 387,993 + Pf6N2E2_320 0.43 +0.3 387,994 - Pf6N2E2_320 0.43 +0.1 388,099 - Pf6N2E2_320 0.48 +0.1 388,099 - Pf6N2E2_320 0.48 -0.4
Or see this region's nucleotide sequence