Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_319

Experiment: perchlorate 50 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_318 and Pf6N2E2_319 are separated by 206 nucleotidesPf6N2E2_319 and Pf6N2E2_320 overlap by 4 nucleotides Pf6N2E2_318 - Gluconolactonase (EC 3.1.1.17), at 385,447 to 386,538 _318 Pf6N2E2_319 - DUF1850 domain-containing protein, at 386,745 to 387,131 _319 Pf6N2E2_320 - TRAP transporter, 4TM/12TM fusion protein, unknown substrate 1, at 387,128 to 389,155 _320 Position (kb) 386 387 388Strain fitness (log2 ratio) -2 -1 0 1at 385.911 kb on + strand, within Pf6N2E2_318at 386.030 kb on - strand, within Pf6N2E2_318at 386.071 kb on + strand, within Pf6N2E2_318at 386.167 kb on + strand, within Pf6N2E2_318at 386.167 kb on + strand, within Pf6N2E2_318at 386.168 kb on - strand, within Pf6N2E2_318at 386.497 kb on + strandat 386.497 kb on + strandat 386.497 kb on + strandat 386.498 kb on - strandat 386.498 kb on - strandat 386.498 kb on - strandat 386.605 kb on - strandat 386.614 kb on - strandat 386.841 kb on + strand, within Pf6N2E2_319at 386.842 kb on - strand, within Pf6N2E2_319at 386.842 kb on - strand, within Pf6N2E2_319at 386.912 kb on - strand, within Pf6N2E2_319at 387.351 kb on + strand, within Pf6N2E2_320at 387.352 kb on - strand, within Pf6N2E2_320at 387.400 kb on - strand, within Pf6N2E2_320at 387.400 kb on - strand, within Pf6N2E2_320at 387.400 kb on - strand, within Pf6N2E2_320at 387.546 kb on + strand, within Pf6N2E2_320at 387.546 kb on + strand, within Pf6N2E2_320at 387.627 kb on + strand, within Pf6N2E2_320at 387.627 kb on + strand, within Pf6N2E2_320at 387.627 kb on + strand, within Pf6N2E2_320at 387.628 kb on - strand, within Pf6N2E2_320at 387.646 kb on + strand, within Pf6N2E2_320at 387.726 kb on + strand, within Pf6N2E2_320at 387.830 kb on + strand, within Pf6N2E2_320at 387.830 kb on + strand, within Pf6N2E2_320at 387.831 kb on - strand, within Pf6N2E2_320at 387.993 kb on + strand, within Pf6N2E2_320at 387.994 kb on - strand, within Pf6N2E2_320at 388.099 kb on - strand, within Pf6N2E2_320at 388.099 kb on - strand, within Pf6N2E2_320

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Per-strain Table

Position Strand Gene LocusTag Fraction perchlorate 50 mM
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385,911 + Pf6N2E2_318 0.42 -0.9
386,030 - Pf6N2E2_318 0.53 -0.3
386,071 + Pf6N2E2_318 0.57 -0.2
386,167 + Pf6N2E2_318 0.66 -0.1
386,167 + Pf6N2E2_318 0.66 +0.2
386,168 - Pf6N2E2_318 0.66 -0.0
386,497 + -0.4
386,497 + +0.4
386,497 + +0.1
386,498 - -1.0
386,498 - -1.6
386,498 - +0.3
386,605 - -1.0
386,614 - -0.5
386,841 + Pf6N2E2_319 0.25 -0.5
386,842 - Pf6N2E2_319 0.25 +0.4
386,842 - Pf6N2E2_319 0.25 -1.2
386,912 - Pf6N2E2_319 0.43 -1.0
387,351 + Pf6N2E2_320 0.11 +0.2
387,352 - Pf6N2E2_320 0.11 +0.0
387,400 - Pf6N2E2_320 0.13 -0.1
387,400 - Pf6N2E2_320 0.13 +0.1
387,400 - Pf6N2E2_320 0.13 -0.4
387,546 + Pf6N2E2_320 0.21 -0.3
387,546 + Pf6N2E2_320 0.21 -0.3
387,627 + Pf6N2E2_320 0.25 -1.8
387,627 + Pf6N2E2_320 0.25 -0.1
387,627 + Pf6N2E2_320 0.25 -0.6
387,628 - Pf6N2E2_320 0.25 -1.7
387,646 + Pf6N2E2_320 0.26 +0.0
387,726 + Pf6N2E2_320 0.29 -0.1
387,830 + Pf6N2E2_320 0.35 -0.1
387,830 + Pf6N2E2_320 0.35 +0.9
387,831 - Pf6N2E2_320 0.35 -0.3
387,993 + Pf6N2E2_320 0.43 +0.3
387,994 - Pf6N2E2_320 0.43 +0.1
388,099 - Pf6N2E2_320 0.48 +0.1
388,099 - Pf6N2E2_320 0.48 -0.4

Or see this region's nucleotide sequence