Experiment: perchlorate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1147 and Pf6N2E2_1148 are separated by 231 nucleotides Pf6N2E2_1148 and Pf6N2E2_1149 are separated by 3 nucleotides
Pf6N2E2_1147 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 1,344,426 to 1,345,199
_1147
Pf6N2E2_1148 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,345,431 to 1,346,582
_1148
Pf6N2E2_1149 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4), at 1,346,586 to 1,347,692
_1149
Position (kb)
1345
1346
1347 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1344.433 kb on - strand at 1344.433 kb on - strand at 1344.433 kb on - strand at 1344.660 kb on + strand, within Pf6N2E2_1147 at 1344.661 kb on - strand, within Pf6N2E2_1147 at 1345.031 kb on + strand, within Pf6N2E2_1147 at 1345.032 kb on - strand at 1345.193 kb on + strand at 1345.193 kb on + strand at 1345.193 kb on + strand at 1345.194 kb on - strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.198 kb on - strand at 1345.423 kb on + strand at 1345.423 kb on + strand at 1345.424 kb on - strand at 1345.441 kb on + strand at 1345.476 kb on + strand at 1345.477 kb on - strand at 1345.495 kb on + strand at 1345.495 kb on + strand at 1345.495 kb on + strand at 1345.496 kb on - strand at 1345.496 kb on - strand at 1345.496 kb on - strand at 1345.522 kb on + strand at 1345.525 kb on + strand at 1345.526 kb on - strand at 1345.526 kb on - strand at 1345.789 kb on + strand, within Pf6N2E2_1148 at 1346.012 kb on + strand, within Pf6N2E2_1148 at 1346.392 kb on + strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.396 kb on + strand, within Pf6N2E2_1148 at 1346.418 kb on + strand, within Pf6N2E2_1148 at 1346.703 kb on + strand, within Pf6N2E2_1149 at 1346.703 kb on + strand, within Pf6N2E2_1149 at 1346.704 kb on - strand, within Pf6N2E2_1149 at 1346.704 kb on - strand, within Pf6N2E2_1149 at 1346.862 kb on + strand, within Pf6N2E2_1149 at 1346.863 kb on - strand, within Pf6N2E2_1149 at 1347.038 kb on + strand, within Pf6N2E2_1149 at 1347.038 kb on + strand, within Pf6N2E2_1149 at 1347.121 kb on + strand, within Pf6N2E2_1149 at 1347.121 kb on + strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.166 kb on + strand, within Pf6N2E2_1149 at 1347.166 kb on + strand, within Pf6N2E2_1149 at 1347.167 kb on - strand, within Pf6N2E2_1149 at 1347.167 kb on - strand, within Pf6N2E2_1149 at 1347.202 kb on + strand, within Pf6N2E2_1149 at 1347.436 kb on + strand, within Pf6N2E2_1149 at 1347.437 kb on - strand, within Pf6N2E2_1149
Per-strain Table
Position Strand Gene LocusTag Fraction perchlorate 50 mM remove 1,344,433 - +0.2 1,344,433 - -0.5 1,344,433 - +0.2 1,344,660 + Pf6N2E2_1147 0.30 +0.4 1,344,661 - Pf6N2E2_1147 0.30 +0.1 1,345,031 + Pf6N2E2_1147 0.78 -0.1 1,345,032 - +2.9 1,345,193 + +0.3 1,345,193 + +0.2 1,345,193 + +0.2 1,345,194 - +0.1 1,345,197 + -0.6 1,345,197 + +0.1 1,345,197 + +3.4 1,345,197 + +0.3 1,345,198 - +0.3 1,345,423 + -0.3 1,345,423 + -0.4 1,345,424 - +0.5 1,345,441 + -0.2 1,345,476 + +0.6 1,345,477 - +0.3 1,345,495 + +0.0 1,345,495 + -0.3 1,345,495 + +2.4 1,345,496 - +1.1 1,345,496 - +0.0 1,345,496 - +0.1 1,345,522 + -1.3 1,345,525 + -0.1 1,345,526 - -0.6 1,345,526 - +0.5 1,345,789 + Pf6N2E2_1148 0.31 +0.6 1,346,012 + Pf6N2E2_1148 0.50 -1.1 1,346,392 + Pf6N2E2_1148 0.83 -0.6 1,346,393 - Pf6N2E2_1148 0.84 -0.6 1,346,393 - Pf6N2E2_1148 0.84 -1.5 1,346,393 - Pf6N2E2_1148 0.84 +0.2 1,346,396 + Pf6N2E2_1148 0.84 -0.4 1,346,418 + Pf6N2E2_1148 0.86 -0.9 1,346,703 + Pf6N2E2_1149 0.11 -0.4 1,346,703 + Pf6N2E2_1149 0.11 -0.3 1,346,704 - Pf6N2E2_1149 0.11 +0.7 1,346,704 - Pf6N2E2_1149 0.11 -0.1 1,346,862 + Pf6N2E2_1149 0.25 -0.5 1,346,863 - Pf6N2E2_1149 0.25 -0.1 1,347,038 + Pf6N2E2_1149 0.41 -0.1 1,347,038 + Pf6N2E2_1149 0.41 -0.4 1,347,121 + Pf6N2E2_1149 0.48 +0.2 1,347,121 + Pf6N2E2_1149 0.48 -1.1 1,347,122 - Pf6N2E2_1149 0.48 -0.2 1,347,122 - Pf6N2E2_1149 0.48 -0.9 1,347,122 - Pf6N2E2_1149 0.48 -1.9 1,347,122 - Pf6N2E2_1149 0.48 -1.0 1,347,122 - Pf6N2E2_1149 0.48 -0.4 1,347,166 + Pf6N2E2_1149 0.52 -0.3 1,347,166 + Pf6N2E2_1149 0.52 -0.8 1,347,167 - Pf6N2E2_1149 0.52 +0.2 1,347,167 - Pf6N2E2_1149 0.52 -0.6 1,347,202 + Pf6N2E2_1149 0.56 -0.1 1,347,436 + Pf6N2E2_1149 0.77 +0.8 1,347,437 - Pf6N2E2_1149 0.77 +0.1
Or see this region's nucleotide sequence