Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08320 and MMJJ_RS08325 are separated by 11 nucleotides MMJJ_RS08325 and MMJJ_RS08330 are separated by 149 nucleotides MMJJ_RS08330 and MMJJ_RS08335 overlap by 31 nucleotides MMJJ_RS08335 and MMJJ_RS08340 are separated by 0 nucleotides
MMJJ_RS08320: MMJJ_RS08320 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,533,989 to 1,534,603
_RS08320
MMJJ_RS08325: MMJJ_RS08325 - glycosyltransferase family 2 protein, at 1,534,615 to 1,535,307
_RS08325
MMJJ_RS08330: MMJJ_RS08330 - SufD family Fe-S cluster assembly protein, at 1,535,457 to 1,536,683
_RS08330
MMJJ_RS08335: MMJJ_RS08335 - ABC transporter ATP-binding protein, at 1,536,653 to 1,537,399
_RS08335
MMJJ_RS08340: MMJJ_RS08340 - archaeoflavoprotein AfpA, at 1,537,400 to 1,537,954
_RS08340
Position (kb) 1535
1536
1537 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1534.553 kb on - strand at 1534.756 kb on - strand, within MMJJ_RS08325 at 1534.782 kb on - strand, within MMJJ_RS08325 at 1534.993 kb on - strand, within MMJJ_RS08325 at 1535.200 kb on - strand, within MMJJ_RS08325 at 1535.200 kb on - strand, within MMJJ_RS08325 at 1535.244 kb on - strand at 1535.268 kb on - strand at 1535.344 kb on - strand at 1535.357 kb on - strand at 1535.357 kb on - strand at 1535.357 kb on - strand at 1535.370 kb on - strand at 1535.370 kb on - strand at 1535.372 kb on - strand at 1535.383 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.389 kb on - strand at 1535.406 kb on - strand at 1535.426 kb on - strand at 1535.427 kb on + strand at 1535.458 kb on - strand at 1535.599 kb on + strand, within MMJJ_RS08330 at 1535.764 kb on + strand, within MMJJ_RS08330 at 1535.783 kb on - strand, within MMJJ_RS08330 at 1535.872 kb on + strand, within MMJJ_RS08330 at 1535.899 kb on + strand, within MMJJ_RS08330 at 1536.153 kb on + strand, within MMJJ_RS08330 at 1536.156 kb on + strand, within MMJJ_RS08330 at 1536.411 kb on + strand, within MMJJ_RS08330 at 1536.805 kb on - strand, within MMJJ_RS08335 at 1536.806 kb on + strand, within MMJJ_RS08335 at 1536.826 kb on - strand, within MMJJ_RS08335 at 1536.977 kb on - strand, within MMJJ_RS08335 at 1537.427 kb on - strand at 1537.454 kb on - strand at 1537.499 kb on - strand, within MMJJ_RS08340 at 1537.517 kb on + strand, within MMJJ_RS08340 at 1537.518 kb on - strand, within MMJJ_RS08340 at 1537.518 kb on - strand, within MMJJ_RS08340 at 1537.520 kb on - strand, within MMJJ_RS08340 at 1537.520 kb on - strand, within MMJJ_RS08340 at 1537.520 kb on - strand, within MMJJ_RS08340 at 1537.521 kb on + strand, within MMJJ_RS08340 at 1537.556 kb on - strand, within MMJJ_RS08340 at 1537.556 kb on - strand, within MMJJ_RS08340 at 1537.591 kb on + strand, within MMJJ_RS08340
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,534,553 - -1.0 1,534,756 - MMJJ_RS08325 0.20 +0.5 1,534,782 - MMJJ_RS08325 0.24 -1.4 1,534,993 - MMJJ_RS08325 0.55 -1.0 1,535,200 - MMJJ_RS08325 0.84 +0.5 1,535,200 - MMJJ_RS08325 0.84 -1.4 1,535,244 - -1.5 1,535,268 - +0.4 1,535,344 - +0.5 1,535,357 - -2.4 1,535,357 - +0.4 1,535,357 - -2.0 1,535,370 - -0.9 1,535,370 - -1.2 1,535,372 - +1.5 1,535,383 + -0.6 1,535,386 + -0.8 1,535,386 + +0.9 1,535,386 + -0.7 1,535,386 + +0.1 1,535,389 - -0.4 1,535,406 - -0.5 1,535,426 - +0.1 1,535,427 + +0.3 1,535,458 - -0.2 1,535,599 + MMJJ_RS08330 0.12 -1.9 1,535,764 + MMJJ_RS08330 0.25 -2.0 1,535,783 - MMJJ_RS08330 0.27 -2.2 1,535,872 + MMJJ_RS08330 0.34 -1.4 1,535,899 + MMJJ_RS08330 0.36 -2.3 1,536,153 + MMJJ_RS08330 0.57 -2.4 1,536,156 + MMJJ_RS08330 0.57 -2.1 1,536,411 + MMJJ_RS08330 0.78 +0.5 1,536,805 - MMJJ_RS08335 0.20 -1.0 1,536,806 + MMJJ_RS08335 0.20 -0.6 1,536,826 - MMJJ_RS08335 0.23 -2.4 1,536,977 - MMJJ_RS08335 0.43 -1.4 1,537,427 - -1.5 1,537,454 - -3.6 1,537,499 - MMJJ_RS08340 0.18 -3.8 1,537,517 + MMJJ_RS08340 0.21 -2.6 1,537,518 - MMJJ_RS08340 0.21 -0.6 1,537,518 - MMJJ_RS08340 0.21 -2.5 1,537,520 - MMJJ_RS08340 0.22 +0.2 1,537,520 - MMJJ_RS08340 0.22 -2.2 1,537,520 - MMJJ_RS08340 0.22 -0.7 1,537,521 + MMJJ_RS08340 0.22 -1.1 1,537,556 - MMJJ_RS08340 0.28 -0.9 1,537,556 - MMJJ_RS08340 0.28 -2.1 1,537,591 + MMJJ_RS08340 0.34 -3.3
Or see this region's nucleotide sequence