Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08235 and MMJJ_RS08240 are separated by 80 nucleotides MMJJ_RS08240 and MMJJ_RS08245 are separated by 28 nucleotides MMJJ_RS08245 and MMJJ_RS08250 are separated by 12 nucleotides MMJJ_RS08250 and MMJJ_RS08255 are separated by 118 nucleotides
MMJJ_RS08235: MMJJ_RS08235 - UPF0147 family protein, at 1,516,380 to 1,516,655
_RS08235
MMJJ_RS08240: MMJJ_RS08240 - L-fuculose phosphate aldolase, at 1,516,736 to 1,517,278
_RS08240
MMJJ_RS08245: MMJJ_RS08245 - ATP-dependent protease LonB, at 1,517,307 to 1,519,385
_RS08245
MMJJ_RS08250: MMJJ_RS08250 - hydrogenase maturation peptidase HycI, at 1,519,398 to 1,519,937
_RS08250
MMJJ_RS08255: MMJJ_RS08255 - GNAT family N-acetyltransferase, at 1,520,056 to 1,520,583
_RS08255
Position (kb)
1517
1518
1519
1520 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1516.317 kb on - strand at 1516.317 kb on - strand at 1516.318 kb on + strand at 1516.318 kb on + strand at 1516.327 kb on - strand at 1516.328 kb on + strand at 1516.328 kb on + strand at 1516.328 kb on + strand at 1516.350 kb on - strand at 1516.354 kb on - strand at 1516.355 kb on + strand at 1516.458 kb on + strand, within MMJJ_RS08235 at 1516.511 kb on + strand, within MMJJ_RS08235 at 1516.511 kb on + strand, within MMJJ_RS08235 at 1516.590 kb on + strand, within MMJJ_RS08235 at 1516.653 kb on - strand at 1516.657 kb on - strand at 1516.672 kb on - strand at 1516.714 kb on - strand at 1516.715 kb on + strand at 1516.722 kb on - strand at 1516.754 kb on + strand at 1516.756 kb on - strand at 1516.756 kb on - strand at 1516.757 kb on + strand at 1516.758 kb on - strand at 1516.759 kb on + strand at 1516.759 kb on + strand at 1516.764 kb on + strand at 1516.782 kb on + strand at 1516.782 kb on + strand at 1516.800 kb on - strand, within MMJJ_RS08240 at 1516.800 kb on - strand, within MMJJ_RS08240 at 1516.801 kb on + strand, within MMJJ_RS08240 at 1516.812 kb on + strand, within MMJJ_RS08240 at 1516.813 kb on - strand, within MMJJ_RS08240 at 1516.829 kb on + strand, within MMJJ_RS08240 at 1516.853 kb on + strand, within MMJJ_RS08240 at 1516.915 kb on + strand, within MMJJ_RS08240 at 1516.915 kb on + strand, within MMJJ_RS08240 at 1516.923 kb on - strand, within MMJJ_RS08240 at 1516.923 kb on - strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.928 kb on - strand, within MMJJ_RS08240 at 1516.970 kb on - strand, within MMJJ_RS08240 at 1517.004 kb on - strand, within MMJJ_RS08240 at 1517.004 kb on - strand, within MMJJ_RS08240 at 1517.005 kb on + strand, within MMJJ_RS08240 at 1517.024 kb on - strand, within MMJJ_RS08240 at 1517.024 kb on - strand, within MMJJ_RS08240 at 1517.052 kb on - strand, within MMJJ_RS08240 at 1517.054 kb on - strand, within MMJJ_RS08240 at 1517.072 kb on - strand, within MMJJ_RS08240 at 1517.073 kb on + strand, within MMJJ_RS08240 at 1517.083 kb on - strand, within MMJJ_RS08240 at 1517.097 kb on + strand, within MMJJ_RS08240 at 1517.156 kb on - strand, within MMJJ_RS08240 at 1517.170 kb on - strand, within MMJJ_RS08240 at 1517.171 kb on + strand, within MMJJ_RS08240 at 1517.171 kb on + strand, within MMJJ_RS08240 at 1517.173 kb on - strand, within MMJJ_RS08240 at 1517.173 kb on - strand, within MMJJ_RS08240 at 1517.173 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.197 kb on - strand, within MMJJ_RS08240 at 1517.201 kb on - strand, within MMJJ_RS08240 at 1517.201 kb on - strand, within MMJJ_RS08240 at 1517.202 kb on + strand, within MMJJ_RS08240 at 1517.226 kb on - strand at 1517.226 kb on - strand at 1517.238 kb on - strand at 1517.238 kb on - strand at 1517.240 kb on - strand at 1517.240 kb on - strand at 1517.244 kb on + strand at 1517.244 kb on + strand at 1517.244 kb on + strand at 1517.259 kb on + strand at 1517.304 kb on + strand at 1517.304 kb on + strand at 1517.304 kb on + strand at 1517.310 kb on - strand at 1517.310 kb on - strand at 1517.311 kb on + strand at 1517.316 kb on + strand at 1517.316 kb on + strand at 1517.316 kb on + strand at 1517.332 kb on + strand at 1518.609 kb on - strand, within MMJJ_RS08245 at 1518.613 kb on + strand, within MMJJ_RS08245 at 1519.041 kb on - strand at 1519.123 kb on + strand, within MMJJ_RS08245 at 1519.464 kb on - strand, within MMJJ_RS08250 at 1519.514 kb on - strand, within MMJJ_RS08250 at 1519.547 kb on - strand, within MMJJ_RS08250 at 1519.547 kb on - strand, within MMJJ_RS08250 at 1519.550 kb on + strand, within MMJJ_RS08250 at 1519.553 kb on - strand, within MMJJ_RS08250 at 1519.557 kb on - strand at 1519.618 kb on + strand, within MMJJ_RS08250 at 1519.627 kb on + strand, within MMJJ_RS08250 at 1519.670 kb on - strand, within MMJJ_RS08250 at 1519.671 kb on + strand, within MMJJ_RS08250 at 1519.671 kb on + strand, within MMJJ_RS08250 at 1519.671 kb on + strand, within MMJJ_RS08250 at 1519.671 kb on + strand, within MMJJ_RS08250 at 1519.699 kb on + strand, within MMJJ_RS08250 at 1519.723 kb on + strand, within MMJJ_RS08250 at 1519.743 kb on - strand, within MMJJ_RS08250 at 1519.752 kb on + strand, within MMJJ_RS08250 at 1519.815 kb on - strand, within MMJJ_RS08250 at 1519.832 kb on + strand, within MMJJ_RS08250 at 1519.857 kb on - strand, within MMJJ_RS08250 at 1519.862 kb on + strand, within MMJJ_RS08250 at 1519.902 kb on + strand at 1519.905 kb on + strand at 1519.914 kb on + strand at 1519.917 kb on + strand at 1519.919 kb on - strand at 1519.920 kb on + strand at 1519.976 kb on - strand at 1519.976 kb on - strand at 1519.976 kb on - strand at 1519.976 kb on - strand at 1519.976 kb on - strand at 1519.977 kb on + strand at 1519.977 kb on + strand at 1519.978 kb on - strand at 1519.978 kb on - strand at 1519.978 kb on - strand at 1519.979 kb on + strand at 1519.979 kb on + strand at 1519.979 kb on + strand at 1519.979 kb on + strand at 1519.980 kb on - strand at 1519.980 kb on - strand at 1519.980 kb on - strand at 1519.980 kb on - strand at 1519.980 kb on - strand at 1519.980 kb on - strand at 1519.980 kb on - strand at 1519.980 kb on - strand at 1519.981 kb on + strand at 1519.981 kb on + strand at 1519.981 kb on + strand at 1519.981 kb on + strand at 1519.982 kb on - strand at 1519.982 kb on - strand at 1519.982 kb on - strand at 1519.982 kb on - strand at 1519.983 kb on + strand at 1519.983 kb on + strand at 1519.983 kb on + strand at 1519.983 kb on + strand at 1519.985 kb on - strand at 1519.986 kb on + strand at 1519.989 kb on - strand at 1520.002 kb on - strand at 1520.003 kb on + strand at 1520.003 kb on + strand at 1520.003 kb on + strand at 1520.005 kb on - strand at 1520.005 kb on - strand at 1520.005 kb on - strand at 1520.006 kb on + strand at 1520.006 kb on + strand at 1520.006 kb on + strand at 1520.007 kb on - strand at 1520.007 kb on - strand at 1520.007 kb on - strand at 1520.007 kb on - strand at 1520.007 kb on - strand at 1520.008 kb on + strand at 1520.008 kb on + strand at 1520.008 kb on + strand at 1520.008 kb on + strand at 1520.008 kb on + strand at 1520.008 kb on + strand at 1520.009 kb on - strand at 1520.009 kb on - strand at 1520.009 kb on - strand at 1520.009 kb on - strand at 1520.010 kb on + strand at 1520.010 kb on + strand at 1520.011 kb on - strand at 1520.012 kb on + strand at 1520.012 kb on + strand at 1520.105 kb on - strand at 1520.105 kb on - strand at 1520.106 kb on + strand at 1520.114 kb on - strand, within MMJJ_RS08255 at 1520.114 kb on - strand, within MMJJ_RS08255 at 1520.120 kb on + strand, within MMJJ_RS08255 at 1520.120 kb on + strand, within MMJJ_RS08255 at 1520.131 kb on + strand, within MMJJ_RS08255 at 1520.140 kb on + strand, within MMJJ_RS08255 at 1520.170 kb on + strand, within MMJJ_RS08255 at 1520.226 kb on + strand, within MMJJ_RS08255 at 1520.232 kb on - strand, within MMJJ_RS08255 at 1520.232 kb on - strand, within MMJJ_RS08255 at 1520.233 kb on + strand, within MMJJ_RS08255 at 1520.233 kb on + strand, within MMJJ_RS08255 at 1520.233 kb on + strand, within MMJJ_RS08255 at 1520.314 kb on + strand, within MMJJ_RS08255 at 1520.319 kb on - strand, within MMJJ_RS08255 at 1520.320 kb on + strand, within MMJJ_RS08255 at 1520.333 kb on - strand, within MMJJ_RS08255 at 1520.333 kb on - strand, within MMJJ_RS08255 at 1520.333 kb on - strand, within MMJJ_RS08255 at 1520.333 kb on - strand, within MMJJ_RS08255 at 1520.334 kb on + strand, within MMJJ_RS08255 at 1520.334 kb on + strand, within MMJJ_RS08255 at 1520.334 kb on + strand, within MMJJ_RS08255 at 1520.336 kb on + strand, within MMJJ_RS08255 at 1520.336 kb on + strand, within MMJJ_RS08255 at 1520.337 kb on - strand, within MMJJ_RS08255 at 1520.337 kb on - strand, within MMJJ_RS08255 at 1520.337 kb on - strand, within MMJJ_RS08255 at 1520.337 kb on - strand, within MMJJ_RS08255 at 1520.337 kb on - strand, within MMJJ_RS08255 at 1520.337 kb on - strand, within MMJJ_RS08255 at 1520.338 kb on + strand, within MMJJ_RS08255 at 1520.338 kb on + strand, within MMJJ_RS08255 at 1520.338 kb on + strand, within MMJJ_RS08255 at 1520.338 kb on + strand, within MMJJ_RS08255 at 1520.338 kb on + strand, within MMJJ_RS08255
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,516,317 - +0.5 1,516,317 - -0.5 1,516,318 + -0.2 1,516,318 + +0.5 1,516,327 - -0.0 1,516,328 + +0.7 1,516,328 + -0.2 1,516,328 + -0.7 1,516,350 - -0.3 1,516,354 - -0.9 1,516,355 + +0.3 1,516,458 + MMJJ_RS08235 0.28 +0.5 1,516,511 + MMJJ_RS08235 0.47 -0.9 1,516,511 + MMJJ_RS08235 0.47 -2.1 1,516,590 + MMJJ_RS08235 0.76 -0.5 1,516,653 - +0.3 1,516,657 - -0.0 1,516,672 - +0.6 1,516,714 - +0.9 1,516,715 + +0.0 1,516,722 - -0.2 1,516,754 + -0.1 1,516,756 - +0.8 1,516,756 - -0.1 1,516,757 + +0.4 1,516,758 - -1.6 1,516,759 + -0.1 1,516,759 + +0.9 1,516,764 + -0.2 1,516,782 + +0.4 1,516,782 + -0.3 1,516,800 - MMJJ_RS08240 0.12 -0.7 1,516,800 - MMJJ_RS08240 0.12 -1.0 1,516,801 + MMJJ_RS08240 0.12 -0.2 1,516,812 + MMJJ_RS08240 0.14 +0.8 1,516,813 - MMJJ_RS08240 0.14 -0.4 1,516,829 + MMJJ_RS08240 0.17 +0.6 1,516,853 + MMJJ_RS08240 0.22 +0.2 1,516,915 + MMJJ_RS08240 0.33 +0.1 1,516,915 + MMJJ_RS08240 0.33 -0.6 1,516,923 - MMJJ_RS08240 0.34 +0.8 1,516,923 - MMJJ_RS08240 0.34 -0.5 1,516,924 + MMJJ_RS08240 0.35 +0.4 1,516,924 + MMJJ_RS08240 0.35 -0.5 1,516,924 + MMJJ_RS08240 0.35 +0.8 1,516,924 + MMJJ_RS08240 0.35 +0.1 1,516,924 + MMJJ_RS08240 0.35 +0.0 1,516,924 + MMJJ_RS08240 0.35 +0.0 1,516,928 - MMJJ_RS08240 0.35 -0.0 1,516,970 - MMJJ_RS08240 0.43 -0.1 1,517,004 - MMJJ_RS08240 0.49 +0.0 1,517,004 - MMJJ_RS08240 0.49 -0.3 1,517,005 + MMJJ_RS08240 0.50 -0.1 1,517,024 - MMJJ_RS08240 0.53 +0.7 1,517,024 - MMJJ_RS08240 0.53 +0.7 1,517,052 - MMJJ_RS08240 0.58 +1.0 1,517,054 - MMJJ_RS08240 0.59 +1.1 1,517,072 - MMJJ_RS08240 0.62 -0.3 1,517,073 + MMJJ_RS08240 0.62 -0.3 1,517,083 - MMJJ_RS08240 0.64 +0.1 1,517,097 + MMJJ_RS08240 0.66 -0.5 1,517,156 - MMJJ_RS08240 0.77 -0.3 1,517,170 - MMJJ_RS08240 0.80 -0.0 1,517,171 + MMJJ_RS08240 0.80 +0.5 1,517,171 + MMJJ_RS08240 0.80 -1.0 1,517,173 - MMJJ_RS08240 0.80 +0.0 1,517,173 - MMJJ_RS08240 0.80 +0.3 1,517,173 - MMJJ_RS08240 0.80 -1.7 1,517,175 - MMJJ_RS08240 0.81 +1.3 1,517,175 - MMJJ_RS08240 0.81 -1.0 1,517,175 - MMJJ_RS08240 0.81 +0.3 1,517,175 - MMJJ_RS08240 0.81 -0.2 1,517,176 + MMJJ_RS08240 0.81 +0.5 1,517,176 + MMJJ_RS08240 0.81 +0.3 1,517,176 + MMJJ_RS08240 0.81 +0.3 1,517,176 + MMJJ_RS08240 0.81 +1.1 1,517,176 + MMJJ_RS08240 0.81 -0.0 1,517,197 - MMJJ_RS08240 0.85 +0.8 1,517,201 - MMJJ_RS08240 0.86 +0.2 1,517,201 - MMJJ_RS08240 0.86 -0.1 1,517,202 + MMJJ_RS08240 0.86 -0.0 1,517,226 - +0.2 1,517,226 - +0.2 1,517,238 - +0.3 1,517,238 - +0.5 1,517,240 - -0.1 1,517,240 - +1.1 1,517,244 + +0.2 1,517,244 + -0.3 1,517,244 + +0.4 1,517,259 + +0.9 1,517,304 + -0.8 1,517,304 + -1.7 1,517,304 + -0.4 1,517,310 - +0.3 1,517,310 - -0.6 1,517,311 + +0.2 1,517,316 + +0.3 1,517,316 + -0.5 1,517,316 + -0.6 1,517,332 + +0.5 1,518,609 - MMJJ_RS08245 0.63 -1.2 1,518,613 + MMJJ_RS08245 0.63 -1.8 1,519,041 - +3.0 1,519,123 + MMJJ_RS08245 0.87 -1.4 1,519,464 - MMJJ_RS08250 0.12 -1.6 1,519,514 - MMJJ_RS08250 0.21 -3.0 1,519,547 - MMJJ_RS08250 0.28 -0.6 1,519,547 - MMJJ_RS08250 0.28 -3.0 1,519,550 + MMJJ_RS08250 0.28 -2.6 1,519,553 - MMJJ_RS08250 0.29 -2.4 1,519,557 - -1.4 1,519,618 + MMJJ_RS08250 0.41 -0.6 1,519,627 + MMJJ_RS08250 0.42 -2.2 1,519,670 - MMJJ_RS08250 0.50 -3.0 1,519,671 + MMJJ_RS08250 0.51 -1.0 1,519,671 + MMJJ_RS08250 0.51 -5.2 1,519,671 + MMJJ_RS08250 0.51 -1.3 1,519,671 + MMJJ_RS08250 0.51 -3.8 1,519,699 + MMJJ_RS08250 0.56 -2.5 1,519,723 + MMJJ_RS08250 0.60 -2.0 1,519,743 - MMJJ_RS08250 0.64 -3.9 1,519,752 + MMJJ_RS08250 0.66 -3.4 1,519,815 - MMJJ_RS08250 0.77 -2.9 1,519,832 + MMJJ_RS08250 0.80 -3.5 1,519,857 - MMJJ_RS08250 0.85 -3.2 1,519,862 + MMJJ_RS08250 0.86 -4.3 1,519,902 + -1.7 1,519,905 + -1.5 1,519,914 + -0.6 1,519,917 + -1.8 1,519,919 - -2.2 1,519,920 + -3.6 1,519,976 - -0.1 1,519,976 - -0.8 1,519,976 - -1.3 1,519,976 - -0.9 1,519,976 - -1.3 1,519,977 + -3.4 1,519,977 + -1.6 1,519,978 - +0.5 1,519,978 - -0.9 1,519,978 - +0.9 1,519,979 + -1.4 1,519,979 + -1.1 1,519,979 + -1.0 1,519,979 + -1.5 1,519,980 - -0.0 1,519,980 - -2.0 1,519,980 - -0.1 1,519,980 - -0.5 1,519,980 - -0.5 1,519,980 - -0.4 1,519,980 - -1.8 1,519,980 - -1.9 1,519,981 + -1.0 1,519,981 + -0.7 1,519,981 + -1.7 1,519,981 + -2.5 1,519,982 - -1.2 1,519,982 - -0.6 1,519,982 - -0.8 1,519,982 - +0.2 1,519,983 + -1.7 1,519,983 + -0.2 1,519,983 + -0.9 1,519,983 + -1.8 1,519,985 - -0.9 1,519,986 + -1.4 1,519,989 - -0.3 1,520,002 - -0.2 1,520,003 + -2.0 1,520,003 + -3.2 1,520,003 + -0.9 1,520,005 - -1.7 1,520,005 - -0.5 1,520,005 - -0.6 1,520,006 + -0.6 1,520,006 + -0.8 1,520,006 + -2.6 1,520,007 - -0.4 1,520,007 - -0.4 1,520,007 - -0.4 1,520,007 - -0.5 1,520,007 - -0.4 1,520,008 + -0.7 1,520,008 + -0.5 1,520,008 + -1.6 1,520,008 + -1.2 1,520,008 + -1.7 1,520,008 + -1.5 1,520,009 - -0.1 1,520,009 - +0.1 1,520,009 - +0.5 1,520,009 - -0.1 1,520,010 + -0.9 1,520,010 + +0.7 1,520,011 - -0.1 1,520,012 + +0.8 1,520,012 + +0.3 1,520,105 - +0.7 1,520,105 - +0.6 1,520,106 + +0.7 1,520,114 - MMJJ_RS08255 0.11 +1.0 1,520,114 - MMJJ_RS08255 0.11 -0.1 1,520,120 + MMJJ_RS08255 0.12 -1.0 1,520,120 + MMJJ_RS08255 0.12 +0.3 1,520,131 + MMJJ_RS08255 0.14 +0.1 1,520,140 + MMJJ_RS08255 0.16 +0.4 1,520,170 + MMJJ_RS08255 0.22 -0.4 1,520,226 + MMJJ_RS08255 0.32 -0.7 1,520,232 - MMJJ_RS08255 0.33 -1.0 1,520,232 - MMJJ_RS08255 0.33 -0.1 1,520,233 + MMJJ_RS08255 0.34 +1.0 1,520,233 + MMJJ_RS08255 0.34 +0.3 1,520,233 + MMJJ_RS08255 0.34 +0.8 1,520,314 + MMJJ_RS08255 0.49 -0.6 1,520,319 - MMJJ_RS08255 0.50 -1.1 1,520,320 + MMJJ_RS08255 0.50 +0.5 1,520,333 - MMJJ_RS08255 0.52 -0.5 1,520,333 - MMJJ_RS08255 0.52 -1.0 1,520,333 - MMJJ_RS08255 0.52 -1.4 1,520,333 - MMJJ_RS08255 0.52 -0.4 1,520,334 + MMJJ_RS08255 0.53 +0.5 1,520,334 + MMJJ_RS08255 0.53 +0.3 1,520,334 + MMJJ_RS08255 0.53 +0.8 1,520,336 + MMJJ_RS08255 0.53 +0.4 1,520,336 + MMJJ_RS08255 0.53 +1.0 1,520,337 - MMJJ_RS08255 0.53 -0.2 1,520,337 - MMJJ_RS08255 0.53 -0.3 1,520,337 - MMJJ_RS08255 0.53 +0.8 1,520,337 - MMJJ_RS08255 0.53 -0.4 1,520,337 - MMJJ_RS08255 0.53 +0.6 1,520,337 - MMJJ_RS08255 0.53 -0.0 1,520,338 + MMJJ_RS08255 0.53 -0.2 1,520,338 + MMJJ_RS08255 0.53 -0.3 1,520,338 + MMJJ_RS08255 0.53 -0.2 1,520,338 + MMJJ_RS08255 0.53 +1.1 1,520,338 + MMJJ_RS08255 0.53 -0.6
Or see this region's nucleotide sequence