Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08150 and MMJJ_RS08155 are separated by 39 nucleotides MMJJ_RS08155 and MMJJ_RS08160 overlap by 23 nucleotides MMJJ_RS08160 and MMJJ_RS08165 are separated by 9 nucleotides
MMJJ_RS08150: MMJJ_RS08150 - M24 family metallopeptidase, at 1,496,798 to 1,497,817
_RS08150
MMJJ_RS08155: MMJJ_RS08155 - cobalt-precorrin-5B (C(1))-methyltransferase CbiD, at 1,497,857 to 1,498,954
_RS08155
MMJJ_RS08160: MMJJ_RS08160 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,498,932 to 1,499,345
_RS08160
MMJJ_RS08165: MMJJ_RS08165 - thermonuclease family protein, at 1,499,355 to 1,499,912
_RS08165
Position (kb)
1497
1498
1499 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1496.861 kb on + strand at 1496.876 kb on + strand at 1496.877 kb on - strand at 1496.877 kb on - strand at 1496.878 kb on + strand at 1496.878 kb on + strand at 1496.898 kb on + strand at 1496.932 kb on - strand, within MMJJ_RS08150 at 1496.932 kb on - strand at 1496.933 kb on + strand, within MMJJ_RS08150 at 1496.941 kb on - strand, within MMJJ_RS08150 at 1496.942 kb on + strand, within MMJJ_RS08150 at 1496.952 kb on - strand, within MMJJ_RS08150 at 1496.952 kb on - strand, within MMJJ_RS08150 at 1496.953 kb on + strand, within MMJJ_RS08150 at 1496.958 kb on - strand, within MMJJ_RS08150 at 1496.991 kb on + strand, within MMJJ_RS08150 at 1497.037 kb on - strand, within MMJJ_RS08150 at 1497.038 kb on + strand, within MMJJ_RS08150 at 1497.038 kb on + strand, within MMJJ_RS08150 at 1497.111 kb on - strand, within MMJJ_RS08150 at 1497.169 kb on - strand, within MMJJ_RS08150 at 1497.217 kb on - strand, within MMJJ_RS08150 at 1497.218 kb on + strand, within MMJJ_RS08150 at 1497.218 kb on + strand, within MMJJ_RS08150 at 1497.218 kb on + strand, within MMJJ_RS08150 at 1497.281 kb on + strand, within MMJJ_RS08150 at 1497.298 kb on - strand, within MMJJ_RS08150 at 1497.298 kb on - strand, within MMJJ_RS08150 at 1497.299 kb on + strand, within MMJJ_RS08150 at 1497.301 kb on - strand, within MMJJ_RS08150 at 1497.301 kb on - strand, within MMJJ_RS08150 at 1497.375 kb on - strand, within MMJJ_RS08150 at 1497.378 kb on - strand, within MMJJ_RS08150 at 1497.413 kb on - strand, within MMJJ_RS08150 at 1497.414 kb on + strand, within MMJJ_RS08150 at 1497.414 kb on + strand, within MMJJ_RS08150 at 1497.433 kb on - strand, within MMJJ_RS08150 at 1497.434 kb on + strand, within MMJJ_RS08150 at 1497.436 kb on - strand, within MMJJ_RS08150 at 1497.447 kb on - strand, within MMJJ_RS08150 at 1497.577 kb on - strand, within MMJJ_RS08150 at 1497.577 kb on - strand, within MMJJ_RS08150 at 1497.578 kb on + strand, within MMJJ_RS08150 at 1497.578 kb on + strand, within MMJJ_RS08150 at 1497.604 kb on + strand, within MMJJ_RS08150 at 1497.604 kb on + strand, within MMJJ_RS08150 at 1497.680 kb on + strand, within MMJJ_RS08150 at 1497.681 kb on - strand, within MMJJ_RS08150 at 1497.685 kb on + strand, within MMJJ_RS08150 at 1497.722 kb on - strand at 1497.722 kb on - strand at 1497.724 kb on - strand at 1497.724 kb on - strand at 1497.759 kb on + strand at 1497.813 kb on + strand at 1497.816 kb on + strand at 1497.820 kb on - strand at 1497.821 kb on + strand at 1497.829 kb on + strand at 1497.833 kb on - strand at 1497.834 kb on + strand at 1497.839 kb on - strand at 1497.856 kb on - strand at 1497.874 kb on - strand at 1497.896 kb on - strand at 1497.902 kb on + strand at 1498.091 kb on + strand, within MMJJ_RS08155 at 1498.120 kb on - strand at 1498.276 kb on + strand, within MMJJ_RS08155 at 1498.276 kb on + strand, within MMJJ_RS08155 at 1498.436 kb on - strand at 1498.497 kb on - strand at 1498.513 kb on - strand, within MMJJ_RS08155 at 1498.589 kb on - strand, within MMJJ_RS08155 at 1498.719 kb on - strand, within MMJJ_RS08155 at 1498.731 kb on + strand, within MMJJ_RS08155 at 1498.740 kb on - strand, within MMJJ_RS08155 at 1498.756 kb on + strand at 1498.787 kb on + strand, within MMJJ_RS08155 at 1498.799 kb on + strand, within MMJJ_RS08155 at 1498.910 kb on + strand at 1498.910 kb on + strand at 1498.913 kb on + strand at 1498.914 kb on - strand at 1498.935 kb on - strand at 1498.935 kb on - strand at 1498.935 kb on - strand at 1498.935 kb on - strand at 1498.978 kb on + strand, within MMJJ_RS08160 at 1499.099 kb on + strand, within MMJJ_RS08160 at 1499.127 kb on + strand, within MMJJ_RS08160 at 1499.135 kb on - strand, within MMJJ_RS08160 at 1499.176 kb on - strand, within MMJJ_RS08160 at 1499.192 kb on - strand, within MMJJ_RS08160 at 1499.229 kb on - strand, within MMJJ_RS08160 at 1499.236 kb on - strand, within MMJJ_RS08160 at 1499.237 kb on + strand, within MMJJ_RS08160 at 1499.241 kb on + strand, within MMJJ_RS08160 at 1499.241 kb on + strand, within MMJJ_RS08160 at 1499.261 kb on - strand, within MMJJ_RS08160 at 1499.268 kb on - strand, within MMJJ_RS08160 at 1499.268 kb on - strand, within MMJJ_RS08160 at 1499.268 kb on - strand, within MMJJ_RS08160 at 1499.268 kb on - strand, within MMJJ_RS08160 at 1499.268 kb on - strand, within MMJJ_RS08160 at 1499.269 kb on + strand, within MMJJ_RS08160 at 1499.269 kb on + strand, within MMJJ_RS08160 at 1499.270 kb on - strand, within MMJJ_RS08160 at 1499.270 kb on - strand, within MMJJ_RS08160 at 1499.271 kb on + strand, within MMJJ_RS08160 at 1499.271 kb on + strand, within MMJJ_RS08160 at 1499.312 kb on - strand at 1499.313 kb on + strand at 1499.315 kb on + strand at 1499.408 kb on - strand at 1499.409 kb on + strand at 1499.431 kb on + strand, within MMJJ_RS08165 at 1499.450 kb on + strand, within MMJJ_RS08165 at 1499.533 kb on + strand, within MMJJ_RS08165 at 1499.534 kb on - strand, within MMJJ_RS08165 at 1499.535 kb on + strand, within MMJJ_RS08165 at 1499.538 kb on + strand, within MMJJ_RS08165 at 1499.549 kb on + strand, within MMJJ_RS08165 at 1499.599 kb on + strand, within MMJJ_RS08165 at 1499.634 kb on - strand, within MMJJ_RS08165 at 1499.670 kb on + strand, within MMJJ_RS08165 at 1499.758 kb on + strand, within MMJJ_RS08165 at 1499.758 kb on + strand, within MMJJ_RS08165 at 1499.760 kb on + strand, within MMJJ_RS08165 at 1499.818 kb on - strand, within MMJJ_RS08165 at 1499.819 kb on + strand, within MMJJ_RS08165 at 1499.822 kb on - strand, within MMJJ_RS08165 at 1499.822 kb on - strand, within MMJJ_RS08165 at 1499.823 kb on + strand, within MMJJ_RS08165 at 1499.902 kb on + strand at 1499.902 kb on + strand at 1499.923 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,496,861 + -0.3 1,496,876 + +0.2 1,496,877 - -0.4 1,496,877 - -0.4 1,496,878 + -1.4 1,496,878 + -0.7 1,496,898 + -0.6 1,496,932 - MMJJ_RS08150 0.13 -1.0 1,496,932 - -0.0 1,496,933 + MMJJ_RS08150 0.13 +0.0 1,496,941 - MMJJ_RS08150 0.14 -1.3 1,496,942 + MMJJ_RS08150 0.14 -0.9 1,496,952 - MMJJ_RS08150 0.15 -0.2 1,496,952 - MMJJ_RS08150 0.15 -3.4 1,496,953 + MMJJ_RS08150 0.15 +0.9 1,496,958 - MMJJ_RS08150 0.16 -0.3 1,496,991 + MMJJ_RS08150 0.19 -0.5 1,497,037 - MMJJ_RS08150 0.23 -0.7 1,497,038 + MMJJ_RS08150 0.24 -0.9 1,497,038 + MMJJ_RS08150 0.24 -0.6 1,497,111 - MMJJ_RS08150 0.31 -0.8 1,497,169 - MMJJ_RS08150 0.36 -0.3 1,497,217 - MMJJ_RS08150 0.41 -1.3 1,497,218 + MMJJ_RS08150 0.41 -0.6 1,497,218 + MMJJ_RS08150 0.41 -0.9 1,497,218 + MMJJ_RS08150 0.41 -0.4 1,497,281 + MMJJ_RS08150 0.47 -1.1 1,497,298 - MMJJ_RS08150 0.49 +0.6 1,497,298 - MMJJ_RS08150 0.49 -1.5 1,497,299 + MMJJ_RS08150 0.49 +0.3 1,497,301 - MMJJ_RS08150 0.49 -0.0 1,497,301 - MMJJ_RS08150 0.49 -0.8 1,497,375 - MMJJ_RS08150 0.57 +0.4 1,497,378 - MMJJ_RS08150 0.57 -1.6 1,497,413 - MMJJ_RS08150 0.60 +0.2 1,497,414 + MMJJ_RS08150 0.60 -1.3 1,497,414 + MMJJ_RS08150 0.60 -2.0 1,497,433 - MMJJ_RS08150 0.62 -0.4 1,497,434 + MMJJ_RS08150 0.62 -1.3 1,497,436 - MMJJ_RS08150 0.63 -1.2 1,497,447 - MMJJ_RS08150 0.64 -0.9 1,497,577 - MMJJ_RS08150 0.76 -0.0 1,497,577 - MMJJ_RS08150 0.76 -0.7 1,497,578 + MMJJ_RS08150 0.76 -0.1 1,497,578 + MMJJ_RS08150 0.76 +0.3 1,497,604 + MMJJ_RS08150 0.79 -0.2 1,497,604 + MMJJ_RS08150 0.79 -0.2 1,497,680 + MMJJ_RS08150 0.86 -1.1 1,497,681 - MMJJ_RS08150 0.87 -0.1 1,497,685 + MMJJ_RS08150 0.87 -0.4 1,497,722 - -0.9 1,497,722 - -0.7 1,497,724 - -0.5 1,497,724 - -1.8 1,497,759 + -0.2 1,497,813 + +1.3 1,497,816 + +0.3 1,497,820 - +0.6 1,497,821 + -0.4 1,497,829 + -0.1 1,497,833 - -0.4 1,497,834 + +0.6 1,497,839 - -1.0 1,497,856 - -1.3 1,497,874 - +0.1 1,497,896 - -1.0 1,497,902 + -0.2 1,498,091 + MMJJ_RS08155 0.21 -2.2 1,498,120 - -1.6 1,498,276 + MMJJ_RS08155 0.38 -1.3 1,498,276 + MMJJ_RS08155 0.38 -0.2 1,498,436 - -0.6 1,498,497 - -2.2 1,498,513 - MMJJ_RS08155 0.60 +1.0 1,498,589 - MMJJ_RS08155 0.67 -3.2 1,498,719 - MMJJ_RS08155 0.79 -2.0 1,498,731 + MMJJ_RS08155 0.80 -0.2 1,498,740 - MMJJ_RS08155 0.80 -2.1 1,498,756 + +2.6 1,498,787 + MMJJ_RS08155 0.85 -3.8 1,498,799 + MMJJ_RS08155 0.86 -0.8 1,498,910 + -0.6 1,498,910 + -1.2 1,498,913 + -1.2 1,498,914 - -1.3 1,498,935 - +0.6 1,498,935 - -0.1 1,498,935 - -1.8 1,498,935 - -0.2 1,498,978 + MMJJ_RS08160 0.11 +0.2 1,499,099 + MMJJ_RS08160 0.40 -0.0 1,499,127 + MMJJ_RS08160 0.47 +0.3 1,499,135 - MMJJ_RS08160 0.49 +0.1 1,499,176 - MMJJ_RS08160 0.59 +0.5 1,499,192 - MMJJ_RS08160 0.63 +2.0 1,499,229 - MMJJ_RS08160 0.72 +0.1 1,499,236 - MMJJ_RS08160 0.73 +0.2 1,499,237 + MMJJ_RS08160 0.74 +0.6 1,499,241 + MMJJ_RS08160 0.75 -0.7 1,499,241 + MMJJ_RS08160 0.75 +0.4 1,499,261 - MMJJ_RS08160 0.79 -0.4 1,499,268 - MMJJ_RS08160 0.81 -0.0 1,499,268 - MMJJ_RS08160 0.81 -0.0 1,499,268 - MMJJ_RS08160 0.81 +0.4 1,499,268 - MMJJ_RS08160 0.81 -0.3 1,499,268 - MMJJ_RS08160 0.81 -0.8 1,499,269 + MMJJ_RS08160 0.81 +1.0 1,499,269 + MMJJ_RS08160 0.81 +0.0 1,499,270 - MMJJ_RS08160 0.82 +0.8 1,499,270 - MMJJ_RS08160 0.82 -1.0 1,499,271 + MMJJ_RS08160 0.82 +0.3 1,499,271 + MMJJ_RS08160 0.82 +1.3 1,499,312 - +0.9 1,499,313 + +1.9 1,499,315 + -0.1 1,499,408 - -0.6 1,499,409 + -1.6 1,499,431 + MMJJ_RS08165 0.14 +1.0 1,499,450 + MMJJ_RS08165 0.17 +0.3 1,499,533 + MMJJ_RS08165 0.32 +0.3 1,499,534 - MMJJ_RS08165 0.32 -0.3 1,499,535 + MMJJ_RS08165 0.32 -0.2 1,499,538 + MMJJ_RS08165 0.33 +0.7 1,499,549 + MMJJ_RS08165 0.35 +0.2 1,499,599 + MMJJ_RS08165 0.44 +0.3 1,499,634 - MMJJ_RS08165 0.50 +0.7 1,499,670 + MMJJ_RS08165 0.56 -0.4 1,499,758 + MMJJ_RS08165 0.72 +0.1 1,499,758 + MMJJ_RS08165 0.72 -0.9 1,499,760 + MMJJ_RS08165 0.73 -0.1 1,499,818 - MMJJ_RS08165 0.83 +0.3 1,499,819 + MMJJ_RS08165 0.83 -1.9 1,499,822 - MMJJ_RS08165 0.84 -1.1 1,499,822 - MMJJ_RS08165 0.84 +0.7 1,499,823 + MMJJ_RS08165 0.84 +1.5 1,499,902 + -0.3 1,499,902 + +0.4 1,499,923 - -0.8
Or see this region's nucleotide sequence