Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08060 and MMJJ_RS08065 are separated by 148 nucleotides MMJJ_RS08065 and MMJJ_RS08070 are separated by 97 nucleotides
MMJJ_RS08060: MMJJ_RS08060 - DNA repair and recombination protein RadA, at 1,476,202 to 1,477,170
_RS08060
MMJJ_RS08065: MMJJ_RS08065 - precorrin-6Y C5,15-methyltransferase (decarboxylating) subunit CbiT, at 1,477,319 to 1,477,864
_RS08065
MMJJ_RS08070: MMJJ_RS08070 - alpha-glucan family phosphorylase, at 1,477,962 to 1,479,524
_RS08070
Position (kb)
1477
1478 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1476.377 kb on - strand, within MMJJ_RS08060 at 1476.378 kb on + strand, within MMJJ_RS08060 at 1476.378 kb on + strand, within MMJJ_RS08060 at 1476.378 kb on + strand, within MMJJ_RS08060 at 1476.378 kb on + strand, within MMJJ_RS08060 at 1476.408 kb on - strand, within MMJJ_RS08060 at 1476.412 kb on - strand, within MMJJ_RS08060 at 1476.412 kb on - strand, within MMJJ_RS08060 at 1476.412 kb on - strand, within MMJJ_RS08060 at 1476.413 kb on + strand, within MMJJ_RS08060 at 1476.428 kb on - strand, within MMJJ_RS08060 at 1476.428 kb on - strand, within MMJJ_RS08060 at 1476.428 kb on - strand, within MMJJ_RS08060 at 1476.430 kb on - strand, within MMJJ_RS08060 at 1476.431 kb on + strand, within MMJJ_RS08060 at 1476.465 kb on - strand, within MMJJ_RS08060 at 1476.531 kb on - strand, within MMJJ_RS08060 at 1476.547 kb on - strand, within MMJJ_RS08060 at 1476.547 kb on - strand, within MMJJ_RS08060 at 1476.551 kb on + strand, within MMJJ_RS08060 at 1476.624 kb on + strand, within MMJJ_RS08060 at 1476.714 kb on - strand, within MMJJ_RS08060 at 1476.787 kb on - strand, within MMJJ_RS08060 at 1476.829 kb on + strand, within MMJJ_RS08060 at 1476.855 kb on - strand, within MMJJ_RS08060 at 1476.856 kb on + strand, within MMJJ_RS08060 at 1476.906 kb on - strand, within MMJJ_RS08060 at 1476.940 kb on - strand, within MMJJ_RS08060 at 1477.020 kb on - strand, within MMJJ_RS08060 at 1477.041 kb on - strand, within MMJJ_RS08060 at 1477.051 kb on + strand, within MMJJ_RS08060 at 1477.170 kb on - strand at 1477.191 kb on - strand at 1477.192 kb on + strand at 1477.203 kb on + strand at 1477.203 kb on + strand at 1477.206 kb on + strand at 1477.206 kb on + strand at 1477.206 kb on + strand at 1477.206 kb on + strand at 1477.208 kb on - strand at 1477.208 kb on - strand at 1477.208 kb on - strand at 1477.208 kb on - strand at 1477.208 kb on - strand at 1477.209 kb on + strand at 1477.209 kb on + strand at 1477.209 kb on + strand at 1477.214 kb on - strand at 1477.215 kb on + strand at 1477.215 kb on + strand at 1477.237 kb on - strand at 1477.248 kb on - strand at 1477.249 kb on + strand at 1477.253 kb on - strand at 1477.253 kb on - strand at 1477.253 kb on - strand at 1477.254 kb on + strand at 1477.254 kb on + strand at 1477.254 kb on + strand at 1477.254 kb on + strand at 1477.258 kb on - strand at 1477.258 kb on - strand at 1477.258 kb on - strand at 1477.259 kb on + strand at 1477.259 kb on + strand at 1477.259 kb on + strand at 1477.259 kb on + strand at 1477.259 kb on + strand at 1477.260 kb on - strand at 1477.260 kb on - strand at 1477.260 kb on - strand at 1477.260 kb on - strand at 1477.260 kb on - strand at 1477.260 kb on - strand at 1477.261 kb on + strand at 1477.261 kb on + strand at 1477.261 kb on + strand at 1477.261 kb on + strand at 1477.265 kb on + strand at 1477.278 kb on + strand at 1477.281 kb on - strand at 1477.283 kb on - strand at 1477.689 kb on - strand, within MMJJ_RS08065 at 1477.770 kb on - strand, within MMJJ_RS08065 at 1477.815 kb on - strand at 1477.863 kb on + strand at 1477.872 kb on - strand at 1477.886 kb on - strand at 1477.888 kb on - strand at 1477.889 kb on + strand at 1477.893 kb on + strand at 1477.896 kb on + strand at 1477.899 kb on - strand at 1477.900 kb on + strand at 1477.900 kb on + strand at 1477.900 kb on + strand at 1477.900 kb on + strand at 1477.901 kb on - strand at 1477.902 kb on + strand at 1477.906 kb on - strand at 1477.906 kb on - strand at 1477.907 kb on + strand at 1477.907 kb on + strand at 1477.915 kb on + strand at 1477.917 kb on - strand at 1477.917 kb on - strand at 1477.918 kb on + strand at 1477.920 kb on - strand at 1477.935 kb on + strand at 1477.941 kb on - strand at 1477.942 kb on + strand at 1477.947 kb on + strand at 1477.977 kb on - strand at 1477.983 kb on + strand at 1478.019 kb on - strand at 1478.020 kb on + strand at 1478.042 kb on + strand at 1478.081 kb on + strand at 1478.081 kb on + strand at 1478.121 kb on - strand, within MMJJ_RS08070 at 1478.151 kb on + strand, within MMJJ_RS08070 at 1478.165 kb on - strand, within MMJJ_RS08070 at 1478.168 kb on + strand, within MMJJ_RS08070 at 1478.175 kb on - strand, within MMJJ_RS08070 at 1478.289 kb on - strand, within MMJJ_RS08070 at 1478.306 kb on - strand, within MMJJ_RS08070 at 1478.352 kb on - strand, within MMJJ_RS08070 at 1478.363 kb on - strand, within MMJJ_RS08070 at 1478.368 kb on - strand, within MMJJ_RS08070 at 1478.445 kb on - strand, within MMJJ_RS08070 at 1478.452 kb on - strand, within MMJJ_RS08070 at 1478.470 kb on - strand, within MMJJ_RS08070 at 1478.512 kb on + strand, within MMJJ_RS08070 at 1478.516 kb on + strand, within MMJJ_RS08070 at 1478.516 kb on + strand, within MMJJ_RS08070 at 1478.527 kb on - strand, within MMJJ_RS08070 at 1478.528 kb on + strand, within MMJJ_RS08070 at 1478.528 kb on + strand, within MMJJ_RS08070 at 1478.535 kb on + strand, within MMJJ_RS08070 at 1478.576 kb on - strand, within MMJJ_RS08070 at 1478.594 kb on - strand, within MMJJ_RS08070 at 1478.762 kb on - strand, within MMJJ_RS08070 at 1478.793 kb on - strand, within MMJJ_RS08070 at 1478.793 kb on - strand, within MMJJ_RS08070 at 1478.793 kb on - strand, within MMJJ_RS08070 at 1478.794 kb on + strand, within MMJJ_RS08070
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,476,377 - MMJJ_RS08060 0.18 -0.5 1,476,378 + MMJJ_RS08060 0.18 +0.5 1,476,378 + MMJJ_RS08060 0.18 -0.3 1,476,378 + MMJJ_RS08060 0.18 +0.6 1,476,378 + MMJJ_RS08060 0.18 -0.4 1,476,408 - MMJJ_RS08060 0.21 -0.8 1,476,412 - MMJJ_RS08060 0.22 -0.9 1,476,412 - MMJJ_RS08060 0.22 -0.0 1,476,412 - MMJJ_RS08060 0.22 -0.1 1,476,413 + MMJJ_RS08060 0.22 -0.7 1,476,428 - MMJJ_RS08060 0.23 +0.6 1,476,428 - MMJJ_RS08060 0.23 -0.5 1,476,428 - MMJJ_RS08060 0.23 -0.5 1,476,430 - MMJJ_RS08060 0.24 -3.8 1,476,431 + MMJJ_RS08060 0.24 +0.3 1,476,465 - MMJJ_RS08060 0.27 -0.6 1,476,531 - MMJJ_RS08060 0.34 -0.5 1,476,547 - MMJJ_RS08060 0.36 +0.0 1,476,547 - MMJJ_RS08060 0.36 -0.0 1,476,551 + MMJJ_RS08060 0.36 +0.3 1,476,624 + MMJJ_RS08060 0.44 +0.0 1,476,714 - MMJJ_RS08060 0.53 -0.0 1,476,787 - MMJJ_RS08060 0.60 -0.2 1,476,829 + MMJJ_RS08060 0.65 -0.0 1,476,855 - MMJJ_RS08060 0.67 +1.1 1,476,856 + MMJJ_RS08060 0.67 +0.0 1,476,906 - MMJJ_RS08060 0.73 -0.3 1,476,940 - MMJJ_RS08060 0.76 +0.3 1,477,020 - MMJJ_RS08060 0.84 +0.4 1,477,041 - MMJJ_RS08060 0.87 +0.6 1,477,051 + MMJJ_RS08060 0.88 +0.4 1,477,170 - -0.0 1,477,191 - -0.0 1,477,192 + -0.7 1,477,203 + -2.2 1,477,203 + +0.1 1,477,206 + -0.4 1,477,206 + -0.3 1,477,206 + +0.4 1,477,206 + +1.1 1,477,208 - -0.6 1,477,208 - -1.2 1,477,208 - -0.6 1,477,208 - +0.2 1,477,208 - -0.2 1,477,209 + -0.2 1,477,209 + -0.2 1,477,209 + -0.6 1,477,214 - -0.9 1,477,215 + +0.0 1,477,215 + +0.8 1,477,237 - +0.6 1,477,248 - +0.1 1,477,249 + +0.1 1,477,253 - +0.6 1,477,253 - +0.4 1,477,253 - +0.0 1,477,254 + -0.0 1,477,254 + -0.1 1,477,254 + -1.6 1,477,254 + -0.3 1,477,258 - +1.1 1,477,258 - +0.8 1,477,258 - +0.5 1,477,259 + +0.9 1,477,259 + -0.3 1,477,259 + -1.2 1,477,259 + -0.6 1,477,259 + +3.0 1,477,260 - -0.5 1,477,260 - +1.1 1,477,260 - +0.8 1,477,260 - +0.6 1,477,260 - -0.2 1,477,260 - -0.2 1,477,261 + -0.0 1,477,261 + +0.6 1,477,261 + +0.2 1,477,261 + +0.1 1,477,265 + -0.1 1,477,278 + -0.2 1,477,281 - -0.1 1,477,283 - +0.1 1,477,689 - MMJJ_RS08065 0.68 -2.8 1,477,770 - MMJJ_RS08065 0.83 -0.9 1,477,815 - -0.0 1,477,863 + -0.3 1,477,872 - +0.9 1,477,886 - -0.2 1,477,888 - +0.1 1,477,889 + -0.3 1,477,893 + -0.3 1,477,896 + +0.4 1,477,899 - +0.2 1,477,900 + +0.2 1,477,900 + +0.8 1,477,900 + +0.3 1,477,900 + +0.3 1,477,901 - +1.0 1,477,902 + -0.2 1,477,906 - -0.3 1,477,906 - -2.0 1,477,907 + +0.1 1,477,907 + +0.7 1,477,915 + +0.1 1,477,917 - -0.3 1,477,917 - -0.8 1,477,918 + -0.5 1,477,920 - +0.6 1,477,935 + -0.1 1,477,941 - -0.0 1,477,942 + -0.4 1,477,947 + +0.5 1,477,977 - +0.2 1,477,983 + -0.4 1,478,019 - +1.3 1,478,020 + +0.6 1,478,042 + -0.6 1,478,081 + -0.4 1,478,081 + +0.5 1,478,121 - MMJJ_RS08070 0.10 +0.7 1,478,151 + MMJJ_RS08070 0.12 +0.2 1,478,165 - MMJJ_RS08070 0.13 -0.3 1,478,168 + MMJJ_RS08070 0.13 -0.0 1,478,175 - MMJJ_RS08070 0.14 -0.3 1,478,289 - MMJJ_RS08070 0.21 -0.1 1,478,306 - MMJJ_RS08070 0.22 +0.2 1,478,352 - MMJJ_RS08070 0.25 -0.4 1,478,363 - MMJJ_RS08070 0.26 +1.4 1,478,368 - MMJJ_RS08070 0.26 -0.2 1,478,445 - MMJJ_RS08070 0.31 +0.4 1,478,452 - MMJJ_RS08070 0.31 +0.3 1,478,470 - MMJJ_RS08070 0.33 +0.3 1,478,512 + MMJJ_RS08070 0.35 -0.1 1,478,516 + MMJJ_RS08070 0.35 +1.0 1,478,516 + MMJJ_RS08070 0.35 -0.3 1,478,527 - MMJJ_RS08070 0.36 -0.5 1,478,528 + MMJJ_RS08070 0.36 +0.4 1,478,528 + MMJJ_RS08070 0.36 +1.5 1,478,535 + MMJJ_RS08070 0.37 +0.1 1,478,576 - MMJJ_RS08070 0.39 +0.8 1,478,594 - MMJJ_RS08070 0.40 -0.3 1,478,762 - MMJJ_RS08070 0.51 +1.4 1,478,793 - MMJJ_RS08070 0.53 +0.7 1,478,793 - MMJJ_RS08070 0.53 -1.0 1,478,793 - MMJJ_RS08070 0.53 -0.3 1,478,794 + MMJJ_RS08070 0.53 +0.2
Or see this region's nucleotide sequence