Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07460 and MMJJ_RS07465 are separated by 10 nucleotides MMJJ_RS07465 and MMJJ_RS07470 are separated by 19 nucleotides MMJJ_RS07470 and MMJJ_RS07475 are separated by 383 nucleotides
MMJJ_RS07460: MMJJ_RS07460 - selenocysteine-specific translation elongation factor, at 1,369,924 to 1,371,330
_RS07460
MMJJ_RS07465: MMJJ_RS07465 - mevalonate kinase, at 1,371,341 to 1,372,294
_RS07465
MMJJ_RS07470: MMJJ_RS07470 - 50S ribosome-binding GTPase, at 1,372,314 to 1,373,468
_RS07470
MMJJ_RS07475: MMJJ_RS07475 - chorismate synthase, at 1,373,852 to 1,374,973
_RS07475
Position (kb)
1372
1373
1374 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1371.316 kb on + strand at 1371.316 kb on + strand at 1371.371 kb on + strand at 1371.566 kb on - strand, within MMJJ_RS07465 at 1372.093 kb on + strand, within MMJJ_RS07465 at 1372.311 kb on + strand at 1372.476 kb on - strand, within MMJJ_RS07470 at 1372.527 kb on - strand, within MMJJ_RS07470 at 1372.686 kb on - strand at 1372.805 kb on + strand, within MMJJ_RS07470 at 1373.257 kb on + strand, within MMJJ_RS07470 at 1373.383 kb on - strand at 1373.383 kb on - strand at 1373.384 kb on + strand at 1373.385 kb on - strand at 1373.429 kb on - strand at 1373.433 kb on + strand at 1373.452 kb on + strand at 1373.460 kb on + strand at 1373.460 kb on + strand at 1373.463 kb on - strand at 1373.464 kb on + strand at 1373.468 kb on - strand at 1373.468 kb on - strand at 1373.468 kb on - strand at 1373.469 kb on + strand at 1373.478 kb on - strand at 1373.478 kb on - strand at 1373.479 kb on + strand at 1373.479 kb on + strand at 1373.481 kb on - strand at 1373.481 kb on - strand at 1373.481 kb on - strand at 1373.481 kb on - strand at 1373.482 kb on + strand at 1373.482 kb on + strand at 1373.482 kb on + strand at 1373.482 kb on + strand at 1373.482 kb on + strand at 1373.482 kb on + strand at 1373.483 kb on - strand at 1373.483 kb on - strand at 1373.483 kb on - strand at 1373.483 kb on - strand at 1373.483 kb on - strand at 1373.484 kb on + strand at 1373.484 kb on + strand at 1373.484 kb on + strand at 1373.484 kb on + strand at 1373.485 kb on - strand at 1373.485 kb on - strand at 1373.486 kb on + strand at 1373.497 kb on - strand at 1373.512 kb on - strand at 1373.514 kb on - strand at 1373.514 kb on - strand at 1373.515 kb on + strand at 1373.553 kb on - strand at 1373.554 kb on + strand at 1373.576 kb on + strand at 1373.587 kb on + strand at 1373.607 kb on - strand at 1373.673 kb on - strand at 1373.673 kb on - strand at 1373.678 kb on - strand at 1373.679 kb on + strand at 1373.679 kb on + strand at 1373.681 kb on + strand at 1373.682 kb on - strand at 1373.682 kb on - strand at 1373.682 kb on - strand at 1373.682 kb on - strand at 1373.682 kb on - strand at 1373.683 kb on + strand at 1373.683 kb on + strand at 1373.683 kb on + strand at 1373.683 kb on + strand at 1373.683 kb on + strand at 1373.684 kb on - strand at 1373.685 kb on + strand at 1373.704 kb on + strand at 1373.705 kb on - strand at 1373.721 kb on + strand at 1373.724 kb on - strand at 1373.729 kb on - strand at 1373.729 kb on - strand at 1373.729 kb on - strand at 1373.729 kb on - strand at 1373.730 kb on + strand at 1373.730 kb on + strand at 1373.730 kb on + strand at 1373.730 kb on + strand at 1373.730 kb on + strand at 1373.730 kb on + strand at 1373.764 kb on - strand at 1373.765 kb on + strand at 1373.853 kb on - strand at 1373.853 kb on - strand at 1373.873 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,371,316 + -1.3 1,371,316 + -0.4 1,371,371 + +0.3 1,371,566 - MMJJ_RS07465 0.24 -1.7 1,372,093 + MMJJ_RS07465 0.79 -2.3 1,372,311 + -0.7 1,372,476 - MMJJ_RS07470 0.14 -2.2 1,372,527 - MMJJ_RS07470 0.18 -1.3 1,372,686 - -1.0 1,372,805 + MMJJ_RS07470 0.43 -0.5 1,373,257 + MMJJ_RS07470 0.82 -1.2 1,373,383 - -0.3 1,373,383 - -0.0 1,373,384 + -1.1 1,373,385 - -0.7 1,373,429 - +0.4 1,373,433 + +0.5 1,373,452 + -0.6 1,373,460 + -1.3 1,373,460 + -0.3 1,373,463 - -0.6 1,373,464 + -0.4 1,373,468 - +0.1 1,373,468 - +0.4 1,373,468 - -0.0 1,373,469 + +0.8 1,373,478 - +0.2 1,373,478 - -0.2 1,373,479 + -0.0 1,373,479 + +0.2 1,373,481 - +0.1 1,373,481 - +0.3 1,373,481 - +1.0 1,373,481 - +0.1 1,373,482 + -1.7 1,373,482 + +0.5 1,373,482 + -0.1 1,373,482 + +0.4 1,373,482 + +0.8 1,373,482 + -0.9 1,373,483 - +0.1 1,373,483 - +0.3 1,373,483 - +1.0 1,373,483 - -0.0 1,373,483 - +1.4 1,373,484 + +0.5 1,373,484 + -0.1 1,373,484 + +0.2 1,373,484 + -0.3 1,373,485 - +0.6 1,373,485 - +1.1 1,373,486 + -0.0 1,373,497 - -2.7 1,373,512 - +0.2 1,373,514 - -0.9 1,373,514 - +1.5 1,373,515 + +1.4 1,373,553 - -0.7 1,373,554 + +1.5 1,373,576 + +0.4 1,373,587 + -3.3 1,373,607 - +0.4 1,373,673 - +0.4 1,373,673 - -0.2 1,373,678 - +1.2 1,373,679 + +0.0 1,373,679 + +0.5 1,373,681 + +0.3 1,373,682 - +0.2 1,373,682 - +0.6 1,373,682 - +0.7 1,373,682 - +0.5 1,373,682 - -1.6 1,373,683 + +1.0 1,373,683 + -1.1 1,373,683 + +0.3 1,373,683 + +0.9 1,373,683 + -0.0 1,373,684 - +0.4 1,373,685 + -0.1 1,373,704 + +0.5 1,373,705 - +0.6 1,373,721 + -0.3 1,373,724 - +1.1 1,373,729 - +1.4 1,373,729 - +0.8 1,373,729 - +0.3 1,373,729 - +0.9 1,373,730 + +0.4 1,373,730 + +0.4 1,373,730 + -0.7 1,373,730 + +0.3 1,373,730 + +0.7 1,373,730 + +0.4 1,373,764 - +0.1 1,373,765 + -0.4 1,373,853 - +0.7 1,373,853 - +1.3 1,373,873 + -1.9
Or see this region's nucleotide sequence