Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07420 and MMJJ_RS07425 are separated by 9 nucleotides MMJJ_RS07425 and MMJJ_RS07430 are separated by 17 nucleotides MMJJ_RS07430 and MMJJ_RS07435 are separated by 9 nucleotides
MMJJ_RS07420: MMJJ_RS07420 - protease complex subunit PrcB family protein, at 1,362,285 to 1,362,752
_RS07420
MMJJ_RS07425: MMJJ_RS07425 - MBL fold metallo-hydrolase, at 1,362,762 to 1,363,562
_RS07425
MMJJ_RS07430: MMJJ_RS07430 - ribonuclease VapC, at 1,363,580 to 1,363,975
_RS07430
MMJJ_RS07435: MMJJ_RS07435 - SMC family ATPase, at 1,363,985 to 1,366,966
_RS07435
Position (kb)
1363
1364 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1362.721 kb on + strand at 1362.809 kb on - strand at 1362.810 kb on + strand at 1362.810 kb on + strand at 1362.811 kb on - strand at 1362.812 kb on + strand at 1362.812 kb on + strand at 1362.839 kb on - strand at 1362.863 kb on - strand, within MMJJ_RS07425 at 1363.093 kb on + strand, within MMJJ_RS07425 at 1363.145 kb on + strand, within MMJJ_RS07425 at 1363.145 kb on + strand, within MMJJ_RS07425 at 1363.165 kb on - strand, within MMJJ_RS07425 at 1363.165 kb on - strand, within MMJJ_RS07425 at 1363.165 kb on - strand, within MMJJ_RS07425 at 1363.166 kb on + strand, within MMJJ_RS07425 at 1363.204 kb on - strand, within MMJJ_RS07425 at 1363.204 kb on - strand, within MMJJ_RS07425 at 1363.204 kb on - strand, within MMJJ_RS07425 at 1363.212 kb on + strand, within MMJJ_RS07425 at 1363.212 kb on + strand, within MMJJ_RS07425 at 1363.212 kb on + strand, within MMJJ_RS07425 at 1363.234 kb on - strand, within MMJJ_RS07425 at 1363.234 kb on - strand, within MMJJ_RS07425 at 1363.244 kb on - strand, within MMJJ_RS07425 at 1363.260 kb on - strand, within MMJJ_RS07425 at 1363.261 kb on + strand, within MMJJ_RS07425 at 1363.261 kb on + strand, within MMJJ_RS07425 at 1363.261 kb on + strand, within MMJJ_RS07425 at 1363.274 kb on + strand, within MMJJ_RS07425 at 1363.315 kb on - strand, within MMJJ_RS07425 at 1363.366 kb on - strand, within MMJJ_RS07425 at 1363.366 kb on - strand, within MMJJ_RS07425 at 1363.367 kb on + strand, within MMJJ_RS07425 at 1363.367 kb on + strand, within MMJJ_RS07425 at 1363.378 kb on - strand, within MMJJ_RS07425 at 1363.417 kb on + strand, within MMJJ_RS07425 at 1363.464 kb on + strand, within MMJJ_RS07425 at 1363.482 kb on + strand, within MMJJ_RS07425 at 1363.529 kb on - strand at 1363.548 kb on - strand at 1363.550 kb on - strand at 1363.550 kb on - strand at 1363.553 kb on - strand at 1363.557 kb on - strand at 1363.582 kb on + strand at 1363.587 kb on + strand at 1363.691 kb on + strand, within MMJJ_RS07430 at 1363.691 kb on + strand, within MMJJ_RS07430 at 1363.714 kb on + strand, within MMJJ_RS07430 at 1363.868 kb on + strand, within MMJJ_RS07430 at 1363.870 kb on + strand, within MMJJ_RS07430 at 1363.871 kb on - strand, within MMJJ_RS07430 at 1363.901 kb on - strand, within MMJJ_RS07430 at 1363.986 kb on - strand at 1363.987 kb on + strand at 1363.987 kb on + strand at 1363.998 kb on + strand at 1364.023 kb on - strand at 1364.031 kb on + strand at 1364.038 kb on - strand at 1364.038 kb on - strand at 1364.038 kb on - strand at 1364.071 kb on - strand at 1364.072 kb on + strand at 1364.148 kb on - strand at 1364.174 kb on + strand at 1364.186 kb on + strand at 1364.188 kb on - strand at 1364.188 kb on - strand at 1364.188 kb on - strand at 1364.201 kb on + strand at 1364.229 kb on + strand at 1364.300 kb on - strand, within MMJJ_RS07435 at 1364.301 kb on + strand, within MMJJ_RS07435 at 1364.373 kb on - strand, within MMJJ_RS07435 at 1364.373 kb on - strand, within MMJJ_RS07435 at 1364.431 kb on - strand, within MMJJ_RS07435 at 1364.507 kb on - strand, within MMJJ_RS07435 at 1364.512 kb on + strand, within MMJJ_RS07435 at 1364.519 kb on + strand, within MMJJ_RS07435 at 1364.592 kb on + strand, within MMJJ_RS07435 at 1364.760 kb on - strand, within MMJJ_RS07435 at 1364.761 kb on + strand, within MMJJ_RS07435 at 1364.800 kb on - strand, within MMJJ_RS07435 at 1364.874 kb on - strand, within MMJJ_RS07435 at 1364.874 kb on - strand, within MMJJ_RS07435 at 1364.875 kb on + strand, within MMJJ_RS07435 at 1364.875 kb on + strand, within MMJJ_RS07435 at 1364.875 kb on + strand, within MMJJ_RS07435
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,362,721 + -0.3 1,362,809 - -0.2 1,362,810 + -0.6 1,362,810 + -1.3 1,362,811 - +0.1 1,362,812 + +0.0 1,362,812 + -1.0 1,362,839 - -0.3 1,362,863 - MMJJ_RS07425 0.13 -2.2 1,363,093 + MMJJ_RS07425 0.41 -1.2 1,363,145 + MMJJ_RS07425 0.48 -0.7 1,363,145 + MMJJ_RS07425 0.48 -0.4 1,363,165 - MMJJ_RS07425 0.50 +0.7 1,363,165 - MMJJ_RS07425 0.50 -2.8 1,363,165 - MMJJ_RS07425 0.50 +0.5 1,363,166 + MMJJ_RS07425 0.50 -1.6 1,363,204 - MMJJ_RS07425 0.55 -0.5 1,363,204 - MMJJ_RS07425 0.55 -0.3 1,363,204 - MMJJ_RS07425 0.55 -1.1 1,363,212 + MMJJ_RS07425 0.56 -3.2 1,363,212 + MMJJ_RS07425 0.56 +0.7 1,363,212 + MMJJ_RS07425 0.56 -0.3 1,363,234 - MMJJ_RS07425 0.59 +0.2 1,363,234 - MMJJ_RS07425 0.59 -0.5 1,363,244 - MMJJ_RS07425 0.60 -1.3 1,363,260 - MMJJ_RS07425 0.62 -2.2 1,363,261 + MMJJ_RS07425 0.62 -0.9 1,363,261 + MMJJ_RS07425 0.62 -0.4 1,363,261 + MMJJ_RS07425 0.62 -0.5 1,363,274 + MMJJ_RS07425 0.64 -0.6 1,363,315 - MMJJ_RS07425 0.69 -0.6 1,363,366 - MMJJ_RS07425 0.75 +0.2 1,363,366 - MMJJ_RS07425 0.75 -0.8 1,363,367 + MMJJ_RS07425 0.76 -0.8 1,363,367 + MMJJ_RS07425 0.76 -1.0 1,363,378 - MMJJ_RS07425 0.77 -3.9 1,363,417 + MMJJ_RS07425 0.82 -1.3 1,363,464 + MMJJ_RS07425 0.88 -2.0 1,363,482 + MMJJ_RS07425 0.90 -1.2 1,363,529 - -0.8 1,363,548 - +0.3 1,363,550 - +0.3 1,363,550 - -0.7 1,363,553 - +1.1 1,363,557 - -0.2 1,363,582 + +0.3 1,363,587 + -0.8 1,363,691 + MMJJ_RS07430 0.28 -0.8 1,363,691 + MMJJ_RS07430 0.28 -2.5 1,363,714 + MMJJ_RS07430 0.34 -2.0 1,363,868 + MMJJ_RS07430 0.73 -2.0 1,363,870 + MMJJ_RS07430 0.73 -2.4 1,363,871 - MMJJ_RS07430 0.73 -0.7 1,363,901 - MMJJ_RS07430 0.81 -1.0 1,363,986 - +0.2 1,363,987 + +1.2 1,363,987 + -1.9 1,363,998 + -0.4 1,364,023 - +0.4 1,364,031 + -0.3 1,364,038 - +1.0 1,364,038 - -1.0 1,364,038 - -0.5 1,364,071 - +0.6 1,364,072 + -0.1 1,364,148 - +0.1 1,364,174 + +0.7 1,364,186 + -0.3 1,364,188 - +0.1 1,364,188 - -0.1 1,364,188 - -0.6 1,364,201 + -0.4 1,364,229 + -0.0 1,364,300 - MMJJ_RS07435 0.11 -0.3 1,364,301 + MMJJ_RS07435 0.11 -0.5 1,364,373 - MMJJ_RS07435 0.13 +0.1 1,364,373 - MMJJ_RS07435 0.13 -0.2 1,364,431 - MMJJ_RS07435 0.15 -0.2 1,364,507 - MMJJ_RS07435 0.18 -0.1 1,364,512 + MMJJ_RS07435 0.18 +0.5 1,364,519 + MMJJ_RS07435 0.18 -0.2 1,364,592 + MMJJ_RS07435 0.20 +0.4 1,364,760 - MMJJ_RS07435 0.26 -0.0 1,364,761 + MMJJ_RS07435 0.26 -0.1 1,364,800 - MMJJ_RS07435 0.27 -1.0 1,364,874 - MMJJ_RS07435 0.30 -0.1 1,364,874 - MMJJ_RS07435 0.30 +0.2 1,364,875 + MMJJ_RS07435 0.30 -0.3 1,364,875 + MMJJ_RS07435 0.30 -0.0 1,364,875 + MMJJ_RS07435 0.30 +0.4
Or see this region's nucleotide sequence