Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07375 and MMJJ_RS07380 are separated by 90 nucleotides MMJJ_RS07380 and MMJJ_RS07385 are separated by 163 nucleotides MMJJ_RS07385 and MMJJ_RS07390 are separated by 6 nucleotides
MMJJ_RS07375: MMJJ_RS07375 - tRNA 2-thiocytidine biosynthesis TtcA family protein, at 1,354,491 to 1,355,465
_RS07375
MMJJ_RS07380: MMJJ_RS07380 - sulfide-dependent adenosine diphosphate thiazole synthase, at 1,355,556 to 1,356,344
_RS07380
MMJJ_RS07385: MMJJ_RS07385 - ribosomal protein S18-alanine N-acetyltransferase, at 1,356,508 to 1,356,993
_RS07385
MMJJ_RS07390: MMJJ_RS07390 - TIGR04014 family B12-binding domain/radical SAM domain-containing protein, at 1,357,000 to 1,358,313
_RS07390
Position (kb)
1355
1356
1357 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1354.560 kb on + strand at 1354.723 kb on - strand, within MMJJ_RS07375 at 1354.828 kb on - strand, within MMJJ_RS07375 at 1354.828 kb on - strand, within MMJJ_RS07375 at 1354.834 kb on - strand, within MMJJ_RS07375 at 1354.835 kb on + strand, within MMJJ_RS07375 at 1354.835 kb on + strand, within MMJJ_RS07375 at 1354.903 kb on - strand, within MMJJ_RS07375 at 1354.907 kb on - strand, within MMJJ_RS07375 at 1355.129 kb on - strand at 1355.150 kb on + strand, within MMJJ_RS07375 at 1355.191 kb on - strand, within MMJJ_RS07375 at 1355.191 kb on - strand, within MMJJ_RS07375 at 1355.212 kb on - strand, within MMJJ_RS07375 at 1355.212 kb on - strand, within MMJJ_RS07375 at 1355.213 kb on + strand, within MMJJ_RS07375 at 1355.231 kb on - strand, within MMJJ_RS07375 at 1355.232 kb on + strand, within MMJJ_RS07375 at 1355.250 kb on + strand, within MMJJ_RS07375 at 1355.281 kb on - strand, within MMJJ_RS07375 at 1355.301 kb on - strand, within MMJJ_RS07375 at 1355.302 kb on + strand, within MMJJ_RS07375 at 1355.302 kb on + strand, within MMJJ_RS07375 at 1355.302 kb on + strand, within MMJJ_RS07375 at 1355.304 kb on - strand, within MMJJ_RS07375 at 1355.305 kb on + strand, within MMJJ_RS07375 at 1355.339 kb on - strand, within MMJJ_RS07375 at 1355.421 kb on + strand at 1355.435 kb on + strand at 1355.435 kb on + strand at 1355.438 kb on - strand at 1355.438 kb on - strand at 1355.439 kb on + strand at 1355.441 kb on - strand at 1355.441 kb on - strand at 1355.441 kb on - strand at 1355.442 kb on + strand at 1355.451 kb on - strand at 1355.452 kb on + strand at 1355.452 kb on + strand at 1355.452 kb on - strand at 1355.453 kb on - strand at 1355.453 kb on - strand at 1355.453 kb on - strand at 1355.466 kb on - strand at 1355.466 kb on - strand at 1355.474 kb on - strand at 1355.477 kb on + strand at 1355.526 kb on - strand at 1355.527 kb on + strand at 1355.534 kb on - strand at 1355.585 kb on + strand at 1355.653 kb on - strand, within MMJJ_RS07380 at 1355.653 kb on - strand at 1355.681 kb on - strand, within MMJJ_RS07380 at 1355.681 kb on - strand, within MMJJ_RS07380 at 1355.816 kb on + strand, within MMJJ_RS07380 at 1355.839 kb on - strand, within MMJJ_RS07380 at 1355.839 kb on - strand, within MMJJ_RS07380 at 1355.903 kb on + strand, within MMJJ_RS07380 at 1355.936 kb on + strand, within MMJJ_RS07380 at 1355.963 kb on + strand, within MMJJ_RS07380 at 1356.035 kb on + strand, within MMJJ_RS07380 at 1356.049 kb on - strand, within MMJJ_RS07380 at 1356.049 kb on - strand, within MMJJ_RS07380 at 1356.061 kb on + strand, within MMJJ_RS07380 at 1356.064 kb on - strand, within MMJJ_RS07380 at 1356.065 kb on + strand, within MMJJ_RS07380 at 1356.067 kb on - strand, within MMJJ_RS07380 at 1356.102 kb on + strand, within MMJJ_RS07380 at 1356.120 kb on + strand, within MMJJ_RS07380 at 1356.188 kb on - strand, within MMJJ_RS07380 at 1356.188 kb on - strand, within MMJJ_RS07380 at 1356.205 kb on - strand, within MMJJ_RS07380 at 1356.206 kb on + strand, within MMJJ_RS07380 at 1356.219 kb on - strand, within MMJJ_RS07380 at 1356.219 kb on - strand, within MMJJ_RS07380 at 1356.220 kb on + strand, within MMJJ_RS07380 at 1356.220 kb on + strand, within MMJJ_RS07380 at 1356.246 kb on - strand, within MMJJ_RS07380 at 1356.288 kb on - strand at 1356.338 kb on - strand at 1356.338 kb on - strand at 1356.339 kb on + strand at 1356.342 kb on - strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.367 kb on - strand at 1356.376 kb on + strand at 1356.404 kb on - strand at 1356.455 kb on + strand at 1356.455 kb on + strand at 1356.458 kb on - strand at 1356.458 kb on - strand at 1356.458 kb on - strand at 1356.459 kb on + strand at 1356.460 kb on - strand at 1356.460 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.464 kb on + strand at 1356.468 kb on + strand at 1356.506 kb on - strand at 1356.506 kb on - strand at 1356.506 kb on - strand at 1356.507 kb on + strand at 1356.515 kb on - strand at 1356.519 kb on - strand at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.615 kb on + strand, within MMJJ_RS07385 at 1356.615 kb on + strand, within MMJJ_RS07385 at 1356.615 kb on + strand, within MMJJ_RS07385 at 1356.616 kb on - strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.641 kb on - strand, within MMJJ_RS07385 at 1356.677 kb on + strand, within MMJJ_RS07385 at 1356.717 kb on + strand, within MMJJ_RS07385 at 1356.735 kb on + strand, within MMJJ_RS07385 at 1356.735 kb on + strand, within MMJJ_RS07385 at 1356.748 kb on - strand, within MMJJ_RS07385 at 1356.749 kb on + strand, within MMJJ_RS07385 at 1356.771 kb on - strand, within MMJJ_RS07385 at 1356.771 kb on - strand, within MMJJ_RS07385 at 1356.772 kb on + strand, within MMJJ_RS07385 at 1356.866 kb on + strand, within MMJJ_RS07385 at 1356.867 kb on - strand, within MMJJ_RS07385 at 1356.867 kb on - strand, within MMJJ_RS07385 at 1356.868 kb on + strand, within MMJJ_RS07385 at 1356.868 kb on + strand, within MMJJ_RS07385 at 1356.878 kb on + strand, within MMJJ_RS07385 at 1356.885 kb on - strand, within MMJJ_RS07385 at 1356.900 kb on - strand, within MMJJ_RS07385 at 1356.901 kb on + strand, within MMJJ_RS07385 at 1356.901 kb on + strand, within MMJJ_RS07385 at 1356.901 kb on + strand, within MMJJ_RS07385 at 1356.904 kb on - strand, within MMJJ_RS07385 at 1356.905 kb on + strand, within MMJJ_RS07385 at 1356.905 kb on + strand, within MMJJ_RS07385 at 1356.907 kb on - strand, within MMJJ_RS07385 at 1356.930 kb on + strand, within MMJJ_RS07385 at 1356.938 kb on - strand, within MMJJ_RS07385 at 1356.938 kb on - strand, within MMJJ_RS07385 at 1356.968 kb on + strand at 1356.991 kb on - strand at 1356.991 kb on - strand at 1356.991 kb on - strand at 1356.991 kb on - strand at 1356.992 kb on + strand at 1356.996 kb on + strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.031 kb on + strand at 1357.031 kb on + strand at 1357.031 kb on + strand at 1357.037 kb on + strand at 1357.037 kb on + strand at 1357.083 kb on - strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.086 kb on - strand at 1357.139 kb on - strand, within MMJJ_RS07390 at 1357.139 kb on - strand, within MMJJ_RS07390 at 1357.139 kb on - strand, within MMJJ_RS07390 at 1357.140 kb on + strand, within MMJJ_RS07390 at 1357.143 kb on - strand, within MMJJ_RS07390 at 1357.144 kb on + strand, within MMJJ_RS07390 at 1357.166 kb on - strand, within MMJJ_RS07390 at 1357.209 kb on + strand, within MMJJ_RS07390 at 1357.271 kb on - strand, within MMJJ_RS07390 at 1357.271 kb on - strand, within MMJJ_RS07390 at 1357.317 kb on - strand, within MMJJ_RS07390 at 1357.318 kb on + strand, within MMJJ_RS07390
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,354,560 + -0.0 1,354,723 - MMJJ_RS07375 0.24 -1.7 1,354,828 - MMJJ_RS07375 0.35 -0.9 1,354,828 - MMJJ_RS07375 0.35 -0.6 1,354,834 - MMJJ_RS07375 0.35 +0.4 1,354,835 + MMJJ_RS07375 0.35 -1.6 1,354,835 + MMJJ_RS07375 0.35 -2.0 1,354,903 - MMJJ_RS07375 0.42 -3.5 1,354,907 - MMJJ_RS07375 0.43 -1.2 1,355,129 - -2.0 1,355,150 + MMJJ_RS07375 0.68 -1.7 1,355,191 - MMJJ_RS07375 0.72 +0.3 1,355,191 - MMJJ_RS07375 0.72 -2.0 1,355,212 - MMJJ_RS07375 0.74 -0.7 1,355,212 - MMJJ_RS07375 0.74 -0.4 1,355,213 + MMJJ_RS07375 0.74 -1.3 1,355,231 - MMJJ_RS07375 0.76 -2.7 1,355,232 + MMJJ_RS07375 0.76 -0.7 1,355,250 + MMJJ_RS07375 0.78 -0.3 1,355,281 - MMJJ_RS07375 0.81 -0.2 1,355,301 - MMJJ_RS07375 0.83 -0.4 1,355,302 + MMJJ_RS07375 0.83 -1.6 1,355,302 + MMJJ_RS07375 0.83 -1.0 1,355,302 + MMJJ_RS07375 0.83 -2.2 1,355,304 - MMJJ_RS07375 0.83 -1.1 1,355,305 + MMJJ_RS07375 0.83 -0.7 1,355,339 - MMJJ_RS07375 0.87 -0.8 1,355,421 + +0.7 1,355,435 + +1.0 1,355,435 + -0.6 1,355,438 - -0.6 1,355,438 - -2.5 1,355,439 + -1.1 1,355,441 - -1.3 1,355,441 - -2.3 1,355,441 - -2.1 1,355,442 + -1.1 1,355,451 - -0.2 1,355,452 + -0.5 1,355,452 + +0.3 1,355,452 - +0.3 1,355,453 - -0.9 1,355,453 - -1.3 1,355,453 - -2.2 1,355,466 - -0.6 1,355,466 - -1.2 1,355,474 - -3.6 1,355,477 + -1.0 1,355,526 - -2.5 1,355,527 + -1.8 1,355,534 - -1.3 1,355,585 + -1.3 1,355,653 - MMJJ_RS07380 0.12 -2.8 1,355,653 - +0.3 1,355,681 - MMJJ_RS07380 0.16 -1.8 1,355,681 - MMJJ_RS07380 0.16 -3.4 1,355,816 + MMJJ_RS07380 0.33 -2.7 1,355,839 - MMJJ_RS07380 0.36 -2.5 1,355,839 - MMJJ_RS07380 0.36 -1.3 1,355,903 + MMJJ_RS07380 0.44 -1.9 1,355,936 + MMJJ_RS07380 0.48 -0.4 1,355,963 + MMJJ_RS07380 0.52 -3.3 1,356,035 + MMJJ_RS07380 0.61 -2.2 1,356,049 - MMJJ_RS07380 0.62 -3.5 1,356,049 - MMJJ_RS07380 0.62 -0.3 1,356,061 + MMJJ_RS07380 0.64 -1.4 1,356,064 - MMJJ_RS07380 0.64 -3.5 1,356,065 + MMJJ_RS07380 0.65 -1.5 1,356,067 - MMJJ_RS07380 0.65 -3.4 1,356,102 + MMJJ_RS07380 0.69 -1.5 1,356,120 + MMJJ_RS07380 0.71 -2.5 1,356,188 - MMJJ_RS07380 0.80 -1.8 1,356,188 - MMJJ_RS07380 0.80 -0.9 1,356,205 - MMJJ_RS07380 0.82 -1.4 1,356,206 + MMJJ_RS07380 0.82 -2.4 1,356,219 - MMJJ_RS07380 0.84 -1.0 1,356,219 - MMJJ_RS07380 0.84 -1.8 1,356,220 + MMJJ_RS07380 0.84 -0.8 1,356,220 + MMJJ_RS07380 0.84 -0.3 1,356,246 - MMJJ_RS07380 0.87 -3.6 1,356,288 - -1.0 1,356,338 - -0.4 1,356,338 - +0.8 1,356,339 + +0.5 1,356,342 - +0.2 1,356,354 + -0.2 1,356,354 + -0.7 1,356,354 + -0.7 1,356,354 + -0.9 1,356,367 - -0.1 1,356,376 + -0.3 1,356,404 - -0.1 1,356,455 + +0.3 1,356,455 + -0.4 1,356,458 - +0.1 1,356,458 - -0.3 1,356,458 - +0.5 1,356,459 + +0.1 1,356,460 - -0.1 1,356,460 - +0.4 1,356,463 - +0.3 1,356,463 - +0.7 1,356,463 - +0.0 1,356,463 - -1.8 1,356,464 + -0.6 1,356,468 + +0.2 1,356,506 - -0.5 1,356,506 - -0.6 1,356,506 - -0.7 1,356,507 + +0.3 1,356,515 - -0.1 1,356,519 - +0.2 1,356,614 - MMJJ_RS07385 0.22 +0.1 1,356,614 - MMJJ_RS07385 0.22 +0.3 1,356,614 - MMJJ_RS07385 0.22 -1.0 1,356,614 - MMJJ_RS07385 0.22 +0.2 1,356,614 - MMJJ_RS07385 0.22 +0.2 1,356,614 - MMJJ_RS07385 0.22 -0.8 1,356,614 - MMJJ_RS07385 0.22 +0.5 1,356,615 + MMJJ_RS07385 0.22 +0.1 1,356,615 + MMJJ_RS07385 0.22 +0.2 1,356,615 + MMJJ_RS07385 0.22 +0.9 1,356,616 - MMJJ_RS07385 0.22 -0.7 1,356,617 + MMJJ_RS07385 0.22 +0.3 1,356,617 + MMJJ_RS07385 0.22 -0.1 1,356,617 + MMJJ_RS07385 0.22 -0.6 1,356,617 + MMJJ_RS07385 0.22 -0.2 1,356,641 - MMJJ_RS07385 0.27 +0.2 1,356,677 + MMJJ_RS07385 0.35 +0.6 1,356,717 + MMJJ_RS07385 0.43 -0.2 1,356,735 + MMJJ_RS07385 0.47 +1.4 1,356,735 + MMJJ_RS07385 0.47 +0.1 1,356,748 - MMJJ_RS07385 0.49 +0.3 1,356,749 + MMJJ_RS07385 0.50 +0.2 1,356,771 - MMJJ_RS07385 0.54 -0.9 1,356,771 - MMJJ_RS07385 0.54 +0.1 1,356,772 + MMJJ_RS07385 0.54 -0.8 1,356,866 + MMJJ_RS07385 0.74 -1.0 1,356,867 - MMJJ_RS07385 0.74 +0.2 1,356,867 - MMJJ_RS07385 0.74 +0.3 1,356,868 + MMJJ_RS07385 0.74 +0.2 1,356,868 + MMJJ_RS07385 0.74 +2.1 1,356,878 + MMJJ_RS07385 0.76 +0.3 1,356,885 - MMJJ_RS07385 0.78 +0.9 1,356,900 - MMJJ_RS07385 0.81 -0.2 1,356,901 + MMJJ_RS07385 0.81 +0.2 1,356,901 + MMJJ_RS07385 0.81 +0.7 1,356,901 + MMJJ_RS07385 0.81 -1.8 1,356,904 - MMJJ_RS07385 0.81 -0.9 1,356,905 + MMJJ_RS07385 0.82 +0.2 1,356,905 + MMJJ_RS07385 0.82 +0.0 1,356,907 - MMJJ_RS07385 0.82 +0.1 1,356,930 + MMJJ_RS07385 0.87 +0.6 1,356,938 - MMJJ_RS07385 0.88 +0.4 1,356,938 - MMJJ_RS07385 0.88 -0.7 1,356,968 + -0.1 1,356,991 - -0.1 1,356,991 - -0.6 1,356,991 - -0.8 1,356,991 - -0.3 1,356,992 + +0.2 1,356,996 + -0.4 1,357,028 - -0.6 1,357,028 - +0.4 1,357,028 - +0.1 1,357,028 - +0.1 1,357,028 - -0.6 1,357,028 - +0.0 1,357,028 - -0.4 1,357,028 - +0.6 1,357,028 - -0.7 1,357,028 - -0.2 1,357,028 - +0.4 1,357,029 + +0.0 1,357,029 + -1.1 1,357,029 + -0.3 1,357,029 + -0.2 1,357,029 + +0.8 1,357,029 + -0.9 1,357,030 - -0.8 1,357,030 - -0.6 1,357,030 - -0.3 1,357,030 - -1.3 1,357,030 - -0.0 1,357,030 - +0.7 1,357,030 - +0.7 1,357,031 + +0.6 1,357,031 + -0.4 1,357,031 + +0.1 1,357,037 + +0.6 1,357,037 + +0.1 1,357,083 - -0.4 1,357,084 + +0.3 1,357,084 + +0.0 1,357,084 + -0.1 1,357,084 + -2.8 1,357,084 + +0.5 1,357,086 - -1.3 1,357,139 - MMJJ_RS07390 0.11 -0.2 1,357,139 - MMJJ_RS07390 0.11 +1.6 1,357,139 - MMJJ_RS07390 0.11 +0.4 1,357,140 + MMJJ_RS07390 0.11 +0.4 1,357,143 - MMJJ_RS07390 0.11 +1.3 1,357,144 + MMJJ_RS07390 0.11 -0.7 1,357,166 - MMJJ_RS07390 0.13 -1.0 1,357,209 + MMJJ_RS07390 0.16 +1.3 1,357,271 - MMJJ_RS07390 0.21 +0.1 1,357,271 - MMJJ_RS07390 0.21 -0.1 1,357,317 - MMJJ_RS07390 0.24 -0.7 1,357,318 + MMJJ_RS07390 0.24 +0.0
Or see this region's nucleotide sequence