Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07335 and MMJJ_RS07340 are separated by 81 nucleotides MMJJ_RS07340 and MMJJ_RS07345 are separated by 253 nucleotides MMJJ_RS07345 and MMJJ_RS07350 are separated by 13 nucleotides MMJJ_RS07350 and MMJJ_RS07355 are separated by 44 nucleotides MMJJ_RS07355 and MMJJ_RS07360 are separated by 15 nucleotides
MMJJ_RS07335: MMJJ_RS07335 - DNA-directed RNA polymerase subunit B'', at 1,347,213 to 1,348,712
_RS07335
MMJJ_RS07340: MMJJ_RS07340 - DNA-directed RNA polymerase subunit H, at 1,348,794 to 1,349,030
_RS07340
MMJJ_RS07345: MMJJ_RS07345 - homocysteine biosynthesis protein, at 1,349,284 to 1,350,825
_RS07345
MMJJ_RS07350: MMJJ_RS07350 - 4Fe-4S binding protein, at 1,350,839 to 1,351,237
_RS07350
MMJJ_RS07355: MMJJ_RS07355 - MoaD/ThiS family protein, at 1,351,282 to 1,351,536
_RS07355
MMJJ_RS07360: MMJJ_RS07360 - TIGR00269 family protein, at 1,351,552 to 1,352,487
_RS07360
Position (kb)
1349
1350
1351 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1348.286 kb on - strand, within MMJJ_RS07335 at 1348.500 kb on - strand, within MMJJ_RS07335 at 1348.753 kb on + strand at 1348.812 kb on - strand at 1349.081 kb on - strand at 1349.087 kb on - strand at 1349.087 kb on - strand at 1349.139 kb on - strand at 1349.148 kb on + strand at 1349.170 kb on - strand at 1349.170 kb on - strand at 1349.171 kb on + strand at 1349.176 kb on - strand at 1349.176 kb on - strand at 1349.177 kb on + strand at 1349.182 kb on - strand at 1349.182 kb on - strand at 1349.183 kb on + strand at 1349.183 kb on + strand at 1349.183 kb on + strand at 1349.183 kb on + strand at 1349.183 kb on + strand at 1349.184 kb on - strand at 1349.184 kb on - strand at 1349.184 kb on - strand at 1349.184 kb on - strand at 1349.185 kb on + strand at 1349.185 kb on + strand at 1349.185 kb on + strand at 1349.185 kb on + strand at 1349.185 kb on + strand at 1349.185 kb on + strand at 1349.187 kb on + strand at 1349.187 kb on + strand at 1349.187 kb on + strand at 1349.187 kb on + strand at 1349.198 kb on - strand at 1349.198 kb on - strand at 1349.199 kb on + strand at 1349.199 kb on + strand at 1349.199 kb on + strand at 1349.199 kb on + strand at 1349.199 kb on + strand at 1349.199 kb on + strand at 1349.199 kb on + strand at 1349.199 kb on + strand at 1349.200 kb on - strand at 1349.200 kb on - strand at 1349.200 kb on - strand at 1349.201 kb on + strand at 1349.201 kb on + strand at 1349.201 kb on + strand at 1349.201 kb on + strand at 1349.201 kb on + strand at 1349.201 kb on + strand at 1349.201 kb on + strand at 1349.201 kb on + strand at 1349.203 kb on + strand at 1349.203 kb on + strand at 1349.203 kb on + strand at 1349.206 kb on + strand at 1349.206 kb on + strand at 1349.206 kb on + strand at 1349.208 kb on + strand at 1349.239 kb on + strand at 1349.402 kb on - strand at 1349.403 kb on + strand at 1349.668 kb on + strand, within MMJJ_RS07345 at 1349.806 kb on - strand, within MMJJ_RS07345 at 1349.807 kb on + strand, within MMJJ_RS07345 at 1350.452 kb on - strand, within MMJJ_RS07345 at 1350.459 kb on - strand, within MMJJ_RS07345 at 1350.460 kb on + strand, within MMJJ_RS07345 at 1350.462 kb on + strand, within MMJJ_RS07345 at 1350.462 kb on + strand, within MMJJ_RS07345 at 1350.652 kb on + strand, within MMJJ_RS07345 at 1350.655 kb on - strand, within MMJJ_RS07345 at 1350.655 kb on - strand, within MMJJ_RS07345 at 1350.655 kb on - strand, within MMJJ_RS07345 at 1350.655 kb on - strand, within MMJJ_RS07345 at 1350.655 kb on - strand at 1350.656 kb on + strand, within MMJJ_RS07345 at 1350.656 kb on + strand, within MMJJ_RS07345 at 1350.656 kb on + strand, within MMJJ_RS07345 at 1350.656 kb on + strand, within MMJJ_RS07345 at 1350.657 kb on - strand, within MMJJ_RS07345 at 1350.657 kb on - strand, within MMJJ_RS07345 at 1350.657 kb on - strand, within MMJJ_RS07345 at 1350.658 kb on + strand, within MMJJ_RS07345 at 1350.658 kb on + strand, within MMJJ_RS07345 at 1350.658 kb on + strand, within MMJJ_RS07345 at 1350.658 kb on + strand, within MMJJ_RS07345 at 1350.659 kb on - strand at 1350.659 kb on - strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.662 kb on - strand, within MMJJ_RS07345 at 1350.684 kb on - strand at 1350.685 kb on + strand at 1350.784 kb on + strand at 1350.879 kb on - strand, within MMJJ_RS07350 at 1350.921 kb on - strand, within MMJJ_RS07350 at 1350.927 kb on - strand, within MMJJ_RS07350 at 1350.927 kb on - strand, within MMJJ_RS07350 at 1350.931 kb on - strand, within MMJJ_RS07350 at 1350.932 kb on + strand, within MMJJ_RS07350 at 1350.937 kb on + strand, within MMJJ_RS07350 at 1351.020 kb on - strand, within MMJJ_RS07350 at 1351.200 kb on - strand at 1351.219 kb on - strand at 1351.225 kb on + strand at 1351.236 kb on + strand at 1351.248 kb on + strand at 1351.272 kb on + strand at 1351.289 kb on - strand at 1351.350 kb on + strand, within MMJJ_RS07355 at 1351.355 kb on - strand, within MMJJ_RS07355 at 1351.409 kb on - strand, within MMJJ_RS07355 at 1351.409 kb on - strand, within MMJJ_RS07355 at 1351.443 kb on + strand, within MMJJ_RS07355 at 1351.443 kb on + strand, within MMJJ_RS07355 at 1351.483 kb on + strand, within MMJJ_RS07355 at 1351.484 kb on - strand, within MMJJ_RS07355 at 1351.484 kb on - strand, within MMJJ_RS07355 at 1351.485 kb on + strand, within MMJJ_RS07355 at 1351.485 kb on + strand, within MMJJ_RS07355 at 1351.485 kb on + strand, within MMJJ_RS07355 at 1351.485 kb on + strand, within MMJJ_RS07355 at 1351.487 kb on - strand, within MMJJ_RS07355 at 1351.487 kb on - strand, within MMJJ_RS07355 at 1351.492 kb on + strand, within MMJJ_RS07355 at 1351.512 kb on + strand at 1351.514 kb on + strand at 1351.514 kb on + strand at 1351.514 kb on + strand at 1351.541 kb on + strand at 1351.583 kb on - strand at 1351.584 kb on + strand at 1351.585 kb on - strand at 1351.585 kb on - strand at 1351.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,348,286 - MMJJ_RS07335 0.72 -1.6 1,348,500 - MMJJ_RS07335 0.86 -1.8 1,348,753 + -1.7 1,348,812 - -1.0 1,349,081 - +0.3 1,349,087 - -3.6 1,349,087 - -1.0 1,349,139 - +0.1 1,349,148 + -0.3 1,349,170 - +0.9 1,349,170 - +0.9 1,349,171 + -0.9 1,349,176 - +0.4 1,349,176 - +0.4 1,349,177 + -0.5 1,349,182 - -0.1 1,349,182 - -1.2 1,349,183 + +0.2 1,349,183 + -0.8 1,349,183 + -0.2 1,349,183 + +0.9 1,349,183 + +1.0 1,349,184 - -1.5 1,349,184 - -3.7 1,349,184 - -3.7 1,349,184 - -1.7 1,349,185 + -1.3 1,349,185 + -2.6 1,349,185 + -0.8 1,349,185 + -0.8 1,349,185 + -1.5 1,349,185 + -0.3 1,349,187 + +0.3 1,349,187 + -1.0 1,349,187 + -1.9 1,349,187 + -0.9 1,349,198 - -1.8 1,349,198 - -0.6 1,349,199 + -0.8 1,349,199 + -1.3 1,349,199 + -2.7 1,349,199 + -2.2 1,349,199 + -0.9 1,349,199 + -2.6 1,349,199 + -2.9 1,349,199 + -2.4 1,349,200 - -0.7 1,349,200 - +0.0 1,349,200 - -2.2 1,349,201 + -4.5 1,349,201 + -1.8 1,349,201 + -1.5 1,349,201 + -2.5 1,349,201 + -1.3 1,349,201 + -2.5 1,349,201 + -2.7 1,349,201 + -0.8 1,349,203 + -3.3 1,349,203 + +0.2 1,349,203 + -1.9 1,349,206 + -2.1 1,349,206 + -2.3 1,349,206 + -2.2 1,349,208 + -2.2 1,349,239 + -2.1 1,349,402 - -1.8 1,349,403 + -1.3 1,349,668 + MMJJ_RS07345 0.25 -2.5 1,349,806 - MMJJ_RS07345 0.34 -1.0 1,349,807 + MMJJ_RS07345 0.34 -0.6 1,350,452 - MMJJ_RS07345 0.76 -1.1 1,350,459 - MMJJ_RS07345 0.76 -3.0 1,350,460 + MMJJ_RS07345 0.76 -0.8 1,350,462 + MMJJ_RS07345 0.76 -1.9 1,350,462 + MMJJ_RS07345 0.76 -2.9 1,350,652 + MMJJ_RS07345 0.89 -2.2 1,350,655 - MMJJ_RS07345 0.89 -3.0 1,350,655 - MMJJ_RS07345 0.89 -2.0 1,350,655 - MMJJ_RS07345 0.89 +0.8 1,350,655 - MMJJ_RS07345 0.89 -0.6 1,350,655 - +0.0 1,350,656 + MMJJ_RS07345 0.89 -0.1 1,350,656 + MMJJ_RS07345 0.89 -2.7 1,350,656 + MMJJ_RS07345 0.89 -0.8 1,350,656 + MMJJ_RS07345 0.89 -1.6 1,350,657 - MMJJ_RS07345 0.89 -1.6 1,350,657 - MMJJ_RS07345 0.89 -1.9 1,350,657 - MMJJ_RS07345 0.89 -1.2 1,350,658 + MMJJ_RS07345 0.89 -3.5 1,350,658 + MMJJ_RS07345 0.89 -2.2 1,350,658 + MMJJ_RS07345 0.89 -3.5 1,350,658 + MMJJ_RS07345 0.89 -1.0 1,350,659 - -1.0 1,350,659 - MMJJ_RS07345 0.89 -2.2 1,350,660 + MMJJ_RS07345 0.89 +0.4 1,350,660 + MMJJ_RS07345 0.89 -1.8 1,350,660 + MMJJ_RS07345 0.89 -0.3 1,350,660 + MMJJ_RS07345 0.89 -0.6 1,350,660 + MMJJ_RS07345 0.89 +0.8 1,350,660 + MMJJ_RS07345 0.89 -1.8 1,350,662 - MMJJ_RS07345 0.89 +1.0 1,350,684 - -0.6 1,350,685 + -1.0 1,350,784 + -3.4 1,350,879 - MMJJ_RS07350 0.10 -4.4 1,350,921 - MMJJ_RS07350 0.21 -4.1 1,350,927 - MMJJ_RS07350 0.22 +0.9 1,350,927 - MMJJ_RS07350 0.22 -0.2 1,350,931 - MMJJ_RS07350 0.23 -1.8 1,350,932 + MMJJ_RS07350 0.23 -0.6 1,350,937 + MMJJ_RS07350 0.25 -0.9 1,351,020 - MMJJ_RS07350 0.45 +0.3 1,351,200 - -3.7 1,351,219 - -1.5 1,351,225 + -1.5 1,351,236 + +0.2 1,351,248 + -1.3 1,351,272 + +0.9 1,351,289 - -2.2 1,351,350 + MMJJ_RS07355 0.27 -1.3 1,351,355 - MMJJ_RS07355 0.29 -1.4 1,351,409 - MMJJ_RS07355 0.50 -0.5 1,351,409 - MMJJ_RS07355 0.50 -1.8 1,351,443 + MMJJ_RS07355 0.63 -2.2 1,351,443 + MMJJ_RS07355 0.63 -1.6 1,351,483 + MMJJ_RS07355 0.79 -4.0 1,351,484 - MMJJ_RS07355 0.79 -0.8 1,351,484 - MMJJ_RS07355 0.79 -0.3 1,351,485 + MMJJ_RS07355 0.80 -3.6 1,351,485 + MMJJ_RS07355 0.80 +0.1 1,351,485 + MMJJ_RS07355 0.80 -1.9 1,351,485 + MMJJ_RS07355 0.80 -1.4 1,351,487 - MMJJ_RS07355 0.80 -1.2 1,351,487 - MMJJ_RS07355 0.80 -1.9 1,351,492 + MMJJ_RS07355 0.82 -0.3 1,351,512 + -3.2 1,351,514 + -2.8 1,351,514 + +0.1 1,351,514 + -0.7 1,351,541 + +2.2 1,351,583 - -0.6 1,351,584 + -1.6 1,351,585 - -1.6 1,351,585 - -3.3 1,351,617 - -1.5
Or see this region's nucleotide sequence