Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07265 and MMJJ_RS07270 overlap by 1 nucleotides MMJJ_RS07270 and MMJJ_RS07275 are separated by 118 nucleotides MMJJ_RS07275 and MMJJ_RS07280 are separated by 63 nucleotides
MMJJ_RS07265: MMJJ_RS07265 - tRNA (adenine-N1)-methyltransferase, at 1,330,815 to 1,331,693
_RS07265
MMJJ_RS07270: MMJJ_RS07270 - ribonuclease HII, at 1,331,693 to 1,332,421
_RS07270
MMJJ_RS07275: MMJJ_RS07275 - formate--phosphoribosylaminoimidazolecarboxamide ligase, at 1,332,540 to 1,333,625
_RS07275
MMJJ_RS07280: MMJJ_RS07280 - phosphomannomutase/phosphoglucomutase, at 1,333,689 to 1,335,002
_RS07280
Position (kb)
1332
1333
1334 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1331.559 kb on + strand, within MMJJ_RS07265 at 1331.568 kb on - strand, within MMJJ_RS07265 at 1331.596 kb on + strand, within MMJJ_RS07265 at 1331.643 kb on - strand at 1331.644 kb on + strand at 1331.670 kb on - strand at 1331.671 kb on + strand at 1331.671 kb on + strand at 1331.671 kb on + strand at 1331.672 kb on - strand at 1331.672 kb on - strand at 1331.694 kb on - strand at 1331.711 kb on - strand at 1331.712 kb on + strand at 1331.890 kb on - strand, within MMJJ_RS07270 at 1331.900 kb on - strand, within MMJJ_RS07270 at 1331.986 kb on - strand, within MMJJ_RS07270 at 1332.029 kb on + strand, within MMJJ_RS07270 at 1332.053 kb on - strand, within MMJJ_RS07270 at 1332.053 kb on - strand, within MMJJ_RS07270 at 1332.055 kb on - strand, within MMJJ_RS07270 at 1332.055 kb on - strand, within MMJJ_RS07270 at 1332.055 kb on - strand, within MMJJ_RS07270 at 1332.056 kb on + strand, within MMJJ_RS07270 at 1332.056 kb on + strand, within MMJJ_RS07270 at 1332.110 kb on - strand, within MMJJ_RS07270 at 1332.119 kb on - strand, within MMJJ_RS07270 at 1332.125 kb on - strand, within MMJJ_RS07270 at 1332.127 kb on - strand, within MMJJ_RS07270 at 1332.127 kb on - strand, within MMJJ_RS07270 at 1332.185 kb on - strand, within MMJJ_RS07270 at 1332.185 kb on - strand, within MMJJ_RS07270 at 1332.203 kb on + strand, within MMJJ_RS07270 at 1332.204 kb on - strand, within MMJJ_RS07270 at 1332.205 kb on + strand, within MMJJ_RS07270 at 1332.268 kb on - strand, within MMJJ_RS07270 at 1332.268 kb on - strand, within MMJJ_RS07270 at 1332.268 kb on - strand, within MMJJ_RS07270 at 1332.268 kb on - strand, within MMJJ_RS07270 at 1332.268 kb on - strand, within MMJJ_RS07270 at 1332.269 kb on + strand, within MMJJ_RS07270 at 1332.269 kb on + strand, within MMJJ_RS07270 at 1332.269 kb on + strand, within MMJJ_RS07270 at 1332.273 kb on + strand, within MMJJ_RS07270 at 1332.273 kb on + strand, within MMJJ_RS07270 at 1332.273 kb on + strand, within MMJJ_RS07270 at 1332.353 kb on - strand at 1332.353 kb on - strand at 1332.396 kb on + strand at 1332.400 kb on - strand at 1332.400 kb on - strand at 1332.403 kb on - strand at 1332.422 kb on - strand at 1332.423 kb on + strand at 1332.423 kb on + strand at 1332.424 kb on - strand at 1332.424 kb on - strand at 1332.425 kb on + strand at 1332.436 kb on - strand at 1332.437 kb on + strand at 1332.440 kb on + strand at 1332.452 kb on - strand at 1332.452 kb on - strand at 1332.453 kb on + strand at 1332.453 kb on + strand at 1332.454 kb on - strand at 1332.475 kb on - strand at 1332.696 kb on + strand, within MMJJ_RS07275 at 1332.735 kb on - strand, within MMJJ_RS07275 at 1332.736 kb on + strand, within MMJJ_RS07275 at 1333.205 kb on + strand, within MMJJ_RS07275 at 1333.217 kb on - strand, within MMJJ_RS07275 at 1333.324 kb on - strand, within MMJJ_RS07275 at 1333.436 kb on + strand, within MMJJ_RS07275 at 1333.436 kb on + strand, within MMJJ_RS07275 at 1333.505 kb on - strand, within MMJJ_RS07275 at 1333.617 kb on + strand at 1333.623 kb on - strand at 1333.624 kb on + strand at 1333.624 kb on + strand at 1333.659 kb on + strand at 1333.673 kb on - strand at 1333.679 kb on - strand at 1333.698 kb on - strand at 1333.719 kb on - strand at 1333.815 kb on + strand at 1334.002 kb on - strand, within MMJJ_RS07280 at 1334.013 kb on - strand, within MMJJ_RS07280 at 1334.050 kb on - strand, within MMJJ_RS07280 at 1334.116 kb on - strand, within MMJJ_RS07280 at 1334.116 kb on - strand, within MMJJ_RS07280 at 1334.117 kb on + strand, within MMJJ_RS07280 at 1334.136 kb on - strand, within MMJJ_RS07280 at 1334.253 kb on - strand, within MMJJ_RS07280 at 1334.254 kb on + strand, within MMJJ_RS07280 at 1334.254 kb on + strand, within MMJJ_RS07280 at 1334.358 kb on - strand, within MMJJ_RS07280 at 1334.358 kb on - strand, within MMJJ_RS07280 at 1334.385 kb on + strand, within MMJJ_RS07280 at 1334.440 kb on + strand, within MMJJ_RS07280 at 1334.444 kb on - strand, within MMJJ_RS07280 at 1334.444 kb on - strand, within MMJJ_RS07280 at 1334.568 kb on - strand, within MMJJ_RS07280
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,331,559 + MMJJ_RS07265 0.85 -2.8 1,331,568 - MMJJ_RS07265 0.86 -2.5 1,331,596 + MMJJ_RS07265 0.89 -3.1 1,331,643 - -2.0 1,331,644 + -2.1 1,331,670 - -0.9 1,331,671 + -1.1 1,331,671 + -0.7 1,331,671 + -1.4 1,331,672 - -1.6 1,331,672 - -1.3 1,331,694 - -1.7 1,331,711 - +0.6 1,331,712 + +0.1 1,331,890 - MMJJ_RS07270 0.27 +0.1 1,331,900 - MMJJ_RS07270 0.28 -0.4 1,331,986 - MMJJ_RS07270 0.40 -0.4 1,332,029 + MMJJ_RS07270 0.46 -0.0 1,332,053 - MMJJ_RS07270 0.49 -0.2 1,332,053 - MMJJ_RS07270 0.49 +0.3 1,332,055 - MMJJ_RS07270 0.50 +0.1 1,332,055 - MMJJ_RS07270 0.50 -2.3 1,332,055 - MMJJ_RS07270 0.50 +0.2 1,332,056 + MMJJ_RS07270 0.50 +0.1 1,332,056 + MMJJ_RS07270 0.50 +0.3 1,332,110 - MMJJ_RS07270 0.57 +0.7 1,332,119 - MMJJ_RS07270 0.58 +1.4 1,332,125 - MMJJ_RS07270 0.59 +0.5 1,332,127 - MMJJ_RS07270 0.60 +0.0 1,332,127 - MMJJ_RS07270 0.60 -0.0 1,332,185 - MMJJ_RS07270 0.67 +2.3 1,332,185 - MMJJ_RS07270 0.67 -2.2 1,332,203 + MMJJ_RS07270 0.70 +0.0 1,332,204 - MMJJ_RS07270 0.70 -0.4 1,332,205 + MMJJ_RS07270 0.70 +0.1 1,332,268 - MMJJ_RS07270 0.79 +0.3 1,332,268 - MMJJ_RS07270 0.79 +0.5 1,332,268 - MMJJ_RS07270 0.79 +0.3 1,332,268 - MMJJ_RS07270 0.79 -0.5 1,332,268 - MMJJ_RS07270 0.79 +0.1 1,332,269 + MMJJ_RS07270 0.79 +1.8 1,332,269 + MMJJ_RS07270 0.79 +0.9 1,332,269 + MMJJ_RS07270 0.79 -0.8 1,332,273 + MMJJ_RS07270 0.80 +0.5 1,332,273 + MMJJ_RS07270 0.80 +0.2 1,332,273 + MMJJ_RS07270 0.80 +0.2 1,332,353 - +0.4 1,332,353 - -0.5 1,332,396 + -0.0 1,332,400 - +0.2 1,332,400 - +0.9 1,332,403 - +1.3 1,332,422 - +0.1 1,332,423 + -0.5 1,332,423 + -0.6 1,332,424 - +0.0 1,332,424 - +1.6 1,332,425 + +0.4 1,332,436 - -0.3 1,332,437 + -0.2 1,332,440 + +0.2 1,332,452 - +0.6 1,332,452 - -0.2 1,332,453 + -1.2 1,332,453 + +0.3 1,332,454 - -0.3 1,332,475 - -1.0 1,332,696 + MMJJ_RS07275 0.14 -1.0 1,332,735 - MMJJ_RS07275 0.18 -0.8 1,332,736 + MMJJ_RS07275 0.18 +0.0 1,333,205 + MMJJ_RS07275 0.61 -1.3 1,333,217 - MMJJ_RS07275 0.62 +0.0 1,333,324 - MMJJ_RS07275 0.72 -1.6 1,333,436 + MMJJ_RS07275 0.83 -3.2 1,333,436 + MMJJ_RS07275 0.83 -1.0 1,333,505 - MMJJ_RS07275 0.89 -0.6 1,333,617 + +0.4 1,333,623 - +0.7 1,333,624 + -0.4 1,333,624 + +0.2 1,333,659 + +0.7 1,333,673 - +0.0 1,333,679 - -0.3 1,333,698 - +0.4 1,333,719 - +0.3 1,333,815 + -0.1 1,334,002 - MMJJ_RS07280 0.24 +0.4 1,334,013 - MMJJ_RS07280 0.25 +0.2 1,334,050 - MMJJ_RS07280 0.27 +0.6 1,334,116 - MMJJ_RS07280 0.32 -0.4 1,334,116 - MMJJ_RS07280 0.32 -0.0 1,334,117 + MMJJ_RS07280 0.33 -1.2 1,334,136 - MMJJ_RS07280 0.34 +0.4 1,334,253 - MMJJ_RS07280 0.43 +0.0 1,334,254 + MMJJ_RS07280 0.43 +0.9 1,334,254 + MMJJ_RS07280 0.43 -1.0 1,334,358 - MMJJ_RS07280 0.51 -0.7 1,334,358 - MMJJ_RS07280 0.51 -0.1 1,334,385 + MMJJ_RS07280 0.53 -0.7 1,334,440 + MMJJ_RS07280 0.57 -0.1 1,334,444 - MMJJ_RS07280 0.57 +0.5 1,334,444 - MMJJ_RS07280 0.57 +0.2 1,334,568 - MMJJ_RS07280 0.67 -0.1
Or see this region's nucleotide sequence