Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06825 and MMJJ_RS06830 are separated by 20 nucleotides MMJJ_RS06830 and MMJJ_RS06835 overlap by 8 nucleotides MMJJ_RS06835 and MMJJ_RS06840 are separated by 28 nucleotides
MMJJ_RS06825: MMJJ_RS06825 - 4Fe-4S binding protein, at 1,260,121 to 1,260,879
_RS06825
MMJJ_RS06830: MMJJ_RS06830 - 4Fe-4S binding protein, at 1,260,900 to 1,261,385
_RS06830
MMJJ_RS06835: MMJJ_RS06835 - 4Fe-4S binding protein, at 1,261,378 to 1,262,559
_RS06835
MMJJ_RS06840: MMJJ_RS06840 - nickel-dependent hydrogenase large subunit, at 1,262,588 to 1,263,715
_RS06840
Position (kb)
1261
1262
1263 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1260.378 kb on - strand, within MMJJ_RS06825 at 1260.379 kb on + strand, within MMJJ_RS06825 at 1260.398 kb on + strand, within MMJJ_RS06825 at 1260.403 kb on - strand, within MMJJ_RS06825 at 1260.403 kb on - strand, within MMJJ_RS06825 at 1260.404 kb on + strand, within MMJJ_RS06825 at 1260.404 kb on + strand, within MMJJ_RS06825 at 1260.475 kb on - strand, within MMJJ_RS06825 at 1260.475 kb on - strand, within MMJJ_RS06825 at 1260.475 kb on - strand, within MMJJ_RS06825 at 1260.505 kb on - strand, within MMJJ_RS06825 at 1260.505 kb on - strand, within MMJJ_RS06825 at 1260.515 kb on + strand, within MMJJ_RS06825 at 1260.529 kb on - strand, within MMJJ_RS06825 at 1260.530 kb on + strand, within MMJJ_RS06825 at 1260.556 kb on - strand, within MMJJ_RS06825 at 1260.557 kb on + strand, within MMJJ_RS06825 at 1260.590 kb on + strand, within MMJJ_RS06825 at 1260.732 kb on - strand, within MMJJ_RS06825 at 1260.733 kb on + strand, within MMJJ_RS06825 at 1260.733 kb on + strand, within MMJJ_RS06825 at 1260.751 kb on - strand, within MMJJ_RS06825 at 1260.751 kb on - strand, within MMJJ_RS06825 at 1260.837 kb on - strand at 1260.874 kb on - strand at 1260.898 kb on - strand at 1260.898 kb on - strand at 1260.902 kb on + strand at 1260.931 kb on + strand at 1260.980 kb on - strand, within MMJJ_RS06830 at 1260.981 kb on + strand, within MMJJ_RS06830 at 1261.025 kb on - strand, within MMJJ_RS06830 at 1261.029 kb on - strand, within MMJJ_RS06830 at 1261.061 kb on + strand, within MMJJ_RS06830 at 1261.061 kb on + strand, within MMJJ_RS06830 at 1261.124 kb on - strand, within MMJJ_RS06830 at 1261.130 kb on - strand, within MMJJ_RS06830 at 1261.191 kb on + strand, within MMJJ_RS06830 at 1261.191 kb on + strand, within MMJJ_RS06830 at 1261.259 kb on - strand, within MMJJ_RS06830 at 1261.260 kb on + strand, within MMJJ_RS06830 at 1261.260 kb on + strand, within MMJJ_RS06830 at 1261.309 kb on - strand, within MMJJ_RS06830 at 1261.309 kb on - strand, within MMJJ_RS06830 at 1261.309 kb on - strand, within MMJJ_RS06830 at 1261.310 kb on + strand, within MMJJ_RS06830 at 1261.310 kb on + strand, within MMJJ_RS06830 at 1261.310 kb on + strand, within MMJJ_RS06830 at 1261.352 kb on + strand at 1261.440 kb on - strand at 1261.454 kb on + strand at 1261.454 kb on + strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.460 kb on + strand at 1261.460 kb on + strand at 1261.460 kb on + strand at 1261.520 kb on + strand, within MMJJ_RS06835 at 1261.535 kb on - strand, within MMJJ_RS06835 at 1261.561 kb on + strand, within MMJJ_RS06835 at 1261.601 kb on + strand, within MMJJ_RS06835 at 1261.601 kb on + strand, within MMJJ_RS06835 at 1261.612 kb on - strand, within MMJJ_RS06835 at 1261.613 kb on + strand, within MMJJ_RS06835 at 1261.613 kb on + strand, within MMJJ_RS06835 at 1261.620 kb on + strand, within MMJJ_RS06835 at 1261.635 kb on + strand, within MMJJ_RS06835 at 1261.661 kb on + strand, within MMJJ_RS06835 at 1261.680 kb on - strand, within MMJJ_RS06835 at 1261.681 kb on + strand, within MMJJ_RS06835 at 1261.692 kb on + strand, within MMJJ_RS06835 at 1261.692 kb on + strand, within MMJJ_RS06835 at 1261.734 kb on + strand, within MMJJ_RS06835 at 1261.822 kb on - strand, within MMJJ_RS06835 at 1261.822 kb on - strand, within MMJJ_RS06835 at 1261.823 kb on + strand, within MMJJ_RS06835 at 1261.840 kb on + strand, within MMJJ_RS06835 at 1261.840 kb on + strand, within MMJJ_RS06835 at 1261.855 kb on - strand, within MMJJ_RS06835 at 1261.855 kb on - strand, within MMJJ_RS06835 at 1261.897 kb on + strand, within MMJJ_RS06835 at 1261.897 kb on + strand, within MMJJ_RS06835 at 1261.897 kb on + strand, within MMJJ_RS06835 at 1261.900 kb on - strand, within MMJJ_RS06835 at 1261.901 kb on + strand, within MMJJ_RS06835 at 1261.901 kb on + strand, within MMJJ_RS06835 at 1261.934 kb on + strand, within MMJJ_RS06835 at 1261.984 kb on + strand, within MMJJ_RS06835 at 1261.986 kb on - strand, within MMJJ_RS06835 at 1262.030 kb on - strand, within MMJJ_RS06835 at 1262.031 kb on + strand, within MMJJ_RS06835 at 1262.035 kb on + strand, within MMJJ_RS06835 at 1262.060 kb on + strand, within MMJJ_RS06835 at 1262.060 kb on + strand, within MMJJ_RS06835 at 1262.060 kb on + strand, within MMJJ_RS06835 at 1262.083 kb on - strand, within MMJJ_RS06835 at 1262.101 kb on - strand, within MMJJ_RS06835 at 1262.101 kb on - strand, within MMJJ_RS06835 at 1262.102 kb on + strand, within MMJJ_RS06835 at 1262.108 kb on - strand, within MMJJ_RS06835 at 1262.109 kb on + strand, within MMJJ_RS06835 at 1262.174 kb on + strand, within MMJJ_RS06835 at 1262.195 kb on + strand, within MMJJ_RS06835 at 1262.195 kb on + strand, within MMJJ_RS06835 at 1262.195 kb on + strand, within MMJJ_RS06835 at 1262.197 kb on - strand, within MMJJ_RS06835 at 1262.197 kb on - strand, within MMJJ_RS06835 at 1262.198 kb on + strand, within MMJJ_RS06835 at 1262.198 kb on + strand, within MMJJ_RS06835 at 1262.306 kb on + strand, within MMJJ_RS06835 at 1262.394 kb on - strand, within MMJJ_RS06835 at 1262.410 kb on - strand, within MMJJ_RS06835 at 1262.411 kb on + strand, within MMJJ_RS06835 at 1262.411 kb on + strand, within MMJJ_RS06835 at 1262.413 kb on + strand, within MMJJ_RS06835 at 1262.469 kb on - strand at 1262.500 kb on + strand at 1262.509 kb on + strand at 1262.541 kb on + strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.563 kb on - strand at 1262.563 kb on - strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.582 kb on - strand at 1262.586 kb on - strand at 1262.617 kb on - strand at 1262.617 kb on - strand at 1262.621 kb on - strand at 1262.621 kb on - strand at 1262.630 kb on - strand at 1262.630 kb on - strand at 1262.638 kb on - strand at 1262.639 kb on + strand at 1262.650 kb on - strand at 1262.650 kb on - strand at 1262.680 kb on + strand at 1262.680 kb on + strand at 1262.680 kb on + strand at 1262.724 kb on - strand, within MMJJ_RS06840 at 1262.725 kb on + strand, within MMJJ_RS06840 at 1262.777 kb on - strand, within MMJJ_RS06840 at 1263.127 kb on - strand, within MMJJ_RS06840 at 1263.271 kb on - strand, within MMJJ_RS06840 at 1263.285 kb on + strand, within MMJJ_RS06840 at 1263.299 kb on + strand, within MMJJ_RS06840 at 1263.430 kb on - strand, within MMJJ_RS06840 at 1263.435 kb on + strand, within MMJJ_RS06840 at 1263.516 kb on - strand, within MMJJ_RS06840 at 1263.516 kb on - strand, within MMJJ_RS06840 at 1263.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,260,378 - MMJJ_RS06825 0.34 -2.9 1,260,379 + MMJJ_RS06825 0.34 +0.5 1,260,398 + MMJJ_RS06825 0.36 -1.6 1,260,403 - MMJJ_RS06825 0.37 -1.7 1,260,403 - MMJJ_RS06825 0.37 -0.7 1,260,404 + MMJJ_RS06825 0.37 -1.2 1,260,404 + MMJJ_RS06825 0.37 -1.6 1,260,475 - MMJJ_RS06825 0.47 -1.9 1,260,475 - MMJJ_RS06825 0.47 -0.5 1,260,475 - MMJJ_RS06825 0.47 -0.7 1,260,505 - MMJJ_RS06825 0.51 -0.5 1,260,505 - MMJJ_RS06825 0.51 -1.2 1,260,515 + MMJJ_RS06825 0.52 -1.5 1,260,529 - MMJJ_RS06825 0.54 -0.0 1,260,530 + MMJJ_RS06825 0.54 -0.6 1,260,556 - MMJJ_RS06825 0.57 +0.4 1,260,557 + MMJJ_RS06825 0.57 -0.6 1,260,590 + MMJJ_RS06825 0.62 -1.8 1,260,732 - MMJJ_RS06825 0.81 +0.1 1,260,733 + MMJJ_RS06825 0.81 +2.0 1,260,733 + MMJJ_RS06825 0.81 -2.2 1,260,751 - MMJJ_RS06825 0.83 +0.4 1,260,751 - MMJJ_RS06825 0.83 -0.7 1,260,837 - -0.7 1,260,874 - -1.1 1,260,898 - -0.7 1,260,898 - -0.4 1,260,902 + -0.9 1,260,931 + +0.3 1,260,980 - MMJJ_RS06830 0.16 -2.2 1,260,981 + MMJJ_RS06830 0.17 -0.6 1,261,025 - MMJJ_RS06830 0.26 +0.1 1,261,029 - MMJJ_RS06830 0.27 -0.4 1,261,061 + MMJJ_RS06830 0.33 -0.7 1,261,061 + MMJJ_RS06830 0.33 +0.2 1,261,124 - MMJJ_RS06830 0.46 -0.6 1,261,130 - MMJJ_RS06830 0.47 -0.5 1,261,191 + MMJJ_RS06830 0.60 -0.8 1,261,191 + MMJJ_RS06830 0.60 -0.3 1,261,259 - MMJJ_RS06830 0.74 +0.1 1,261,260 + MMJJ_RS06830 0.74 -0.7 1,261,260 + MMJJ_RS06830 0.74 -1.9 1,261,309 - MMJJ_RS06830 0.84 +0.1 1,261,309 - MMJJ_RS06830 0.84 -0.2 1,261,309 - MMJJ_RS06830 0.84 -1.0 1,261,310 + MMJJ_RS06830 0.84 -0.8 1,261,310 + MMJJ_RS06830 0.84 -0.5 1,261,310 + MMJJ_RS06830 0.84 -0.5 1,261,352 + -2.3 1,261,440 - -0.8 1,261,454 + +0.3 1,261,454 + +0.0 1,261,459 - -3.3 1,261,459 - -0.3 1,261,459 - -0.4 1,261,459 - -1.3 1,261,459 - -1.4 1,261,460 + -2.0 1,261,460 + -0.3 1,261,460 + -1.2 1,261,520 + MMJJ_RS06835 0.12 -2.0 1,261,535 - MMJJ_RS06835 0.13 -0.0 1,261,561 + MMJJ_RS06835 0.15 +0.3 1,261,601 + MMJJ_RS06835 0.19 -2.8 1,261,601 + MMJJ_RS06835 0.19 -1.7 1,261,612 - MMJJ_RS06835 0.20 -2.7 1,261,613 + MMJJ_RS06835 0.20 -3.3 1,261,613 + MMJJ_RS06835 0.20 -2.8 1,261,620 + MMJJ_RS06835 0.20 -3.6 1,261,635 + MMJJ_RS06835 0.22 -2.6 1,261,661 + MMJJ_RS06835 0.24 -2.5 1,261,680 - MMJJ_RS06835 0.26 -0.3 1,261,681 + MMJJ_RS06835 0.26 -2.0 1,261,692 + MMJJ_RS06835 0.27 -2.4 1,261,692 + MMJJ_RS06835 0.27 -2.5 1,261,734 + MMJJ_RS06835 0.30 -1.6 1,261,822 - MMJJ_RS06835 0.38 -1.4 1,261,822 - MMJJ_RS06835 0.38 -2.5 1,261,823 + MMJJ_RS06835 0.38 -2.2 1,261,840 + MMJJ_RS06835 0.39 -0.1 1,261,840 + MMJJ_RS06835 0.39 -3.0 1,261,855 - MMJJ_RS06835 0.40 -1.7 1,261,855 - MMJJ_RS06835 0.40 -1.6 1,261,897 + MMJJ_RS06835 0.44 -2.7 1,261,897 + MMJJ_RS06835 0.44 -3.7 1,261,897 + MMJJ_RS06835 0.44 -2.3 1,261,900 - MMJJ_RS06835 0.44 -3.1 1,261,901 + MMJJ_RS06835 0.44 -1.9 1,261,901 + MMJJ_RS06835 0.44 -1.6 1,261,934 + MMJJ_RS06835 0.47 -2.3 1,261,984 + MMJJ_RS06835 0.51 -1.9 1,261,986 - MMJJ_RS06835 0.51 -1.5 1,262,030 - MMJJ_RS06835 0.55 -1.8 1,262,031 + MMJJ_RS06835 0.55 -1.6 1,262,035 + MMJJ_RS06835 0.56 -2.8 1,262,060 + MMJJ_RS06835 0.58 -2.1 1,262,060 + MMJJ_RS06835 0.58 -3.4 1,262,060 + MMJJ_RS06835 0.58 -0.8 1,262,083 - MMJJ_RS06835 0.60 -3.4 1,262,101 - MMJJ_RS06835 0.61 -2.3 1,262,101 - MMJJ_RS06835 0.61 -2.0 1,262,102 + MMJJ_RS06835 0.61 -2.3 1,262,108 - MMJJ_RS06835 0.62 -1.9 1,262,109 + MMJJ_RS06835 0.62 -3.0 1,262,174 + MMJJ_RS06835 0.67 -3.0 1,262,195 + MMJJ_RS06835 0.69 -2.6 1,262,195 + MMJJ_RS06835 0.69 -3.7 1,262,195 + MMJJ_RS06835 0.69 -1.5 1,262,197 - MMJJ_RS06835 0.69 -2.6 1,262,197 - MMJJ_RS06835 0.69 -1.7 1,262,198 + MMJJ_RS06835 0.69 -2.4 1,262,198 + MMJJ_RS06835 0.69 -2.0 1,262,306 + MMJJ_RS06835 0.79 -3.3 1,262,394 - MMJJ_RS06835 0.86 -3.8 1,262,410 - MMJJ_RS06835 0.87 -2.1 1,262,411 + MMJJ_RS06835 0.87 -1.7 1,262,411 + MMJJ_RS06835 0.87 -4.5 1,262,413 + MMJJ_RS06835 0.88 -1.8 1,262,469 - -0.9 1,262,500 + -0.5 1,262,509 + -0.9 1,262,541 + -2.2 1,262,559 - -1.0 1,262,559 - -2.9 1,262,559 - -2.1 1,262,559 - -3.1 1,262,559 - -1.7 1,262,559 - -3.3 1,262,559 - -1.6 1,262,559 - +0.1 1,262,560 + -4.1 1,262,560 + -2.9 1,262,560 + -4.3 1,262,560 + -4.0 1,262,560 + -2.7 1,262,560 + -2.3 1,262,560 + +0.3 1,262,563 - -2.6 1,262,563 - -3.3 1,262,564 + -2.8 1,262,564 + -2.0 1,262,564 + -2.7 1,262,564 + -3.1 1,262,564 + -1.9 1,262,582 - -3.6 1,262,586 - -1.5 1,262,617 - -1.2 1,262,617 - -2.9 1,262,621 - -1.9 1,262,621 - -2.6 1,262,630 - -3.4 1,262,630 - -3.2 1,262,638 - -2.5 1,262,639 + -4.3 1,262,650 - -2.0 1,262,650 - -2.5 1,262,680 + -2.0 1,262,680 + -2.4 1,262,680 + -0.5 1,262,724 - MMJJ_RS06840 0.12 -3.7 1,262,725 + MMJJ_RS06840 0.12 -3.1 1,262,777 - MMJJ_RS06840 0.17 -2.9 1,263,127 - MMJJ_RS06840 0.48 -1.5 1,263,271 - MMJJ_RS06840 0.61 -0.5 1,263,285 + MMJJ_RS06840 0.62 -2.4 1,263,299 + MMJJ_RS06840 0.63 -2.0 1,263,430 - MMJJ_RS06840 0.75 -2.1 1,263,435 + MMJJ_RS06840 0.75 -2.1 1,263,516 - MMJJ_RS06840 0.82 -0.8 1,263,516 - MMJJ_RS06840 0.82 -1.0 1,263,553 - +0.0
Or see this region's nucleotide sequence