Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06170 and MMJJ_RS06175 are separated by 11 nucleotides MMJJ_RS06175 and MMJJ_RS06180 are separated by 27 nucleotides MMJJ_RS06180 and MMJJ_RS06185 are separated by 36 nucleotides
MMJJ_RS06170: MMJJ_RS06170 - hypothetical protein, at 1,144,789 to 1,145,331
_RS06170
MMJJ_RS06175: MMJJ_RS06175 - DUF1743 domain-containing protein, at 1,145,343 to 1,146,248
_RS06175
MMJJ_RS06180: MMJJ_RS06180 - tryptophan--tRNA ligase, at 1,146,276 to 1,147,352
_RS06180
MMJJ_RS06185: MMJJ_RS06185 - cobalamin biosynthesis protein, at 1,147,389 to 1,148,360
_RS06185
Position (kb)
1146
1147
1148 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1145.301 kb on - strand at 1145.309 kb on + strand at 1145.309 kb on + strand at 1145.330 kb on + strand at 1145.330 kb on + strand at 1145.507 kb on - strand, within MMJJ_RS06175 at 1145.678 kb on + strand at 1145.824 kb on - strand, within MMJJ_RS06175 at 1145.962 kb on + strand, within MMJJ_RS06175 at 1145.962 kb on + strand, within MMJJ_RS06175 at 1146.045 kb on + strand, within MMJJ_RS06175 at 1146.185 kb on + strand at 1146.562 kb on - strand, within MMJJ_RS06180 at 1146.563 kb on + strand at 1146.563 kb on + strand, within MMJJ_RS06180 at 1146.564 kb on - strand, within MMJJ_RS06180 at 1146.564 kb on - strand, within MMJJ_RS06180 at 1146.564 kb on - strand, within MMJJ_RS06180 at 1146.565 kb on + strand at 1146.658 kb on + strand, within MMJJ_RS06180 at 1146.697 kb on + strand at 1146.842 kb on - strand, within MMJJ_RS06180 at 1147.174 kb on + strand, within MMJJ_RS06180 at 1147.394 kb on + strand at 1147.405 kb on + strand at 1147.441 kb on - strand at 1147.441 kb on - strand at 1147.461 kb on - strand at 1147.490 kb on + strand, within MMJJ_RS06185 at 1147.505 kb on + strand, within MMJJ_RS06185 at 1147.532 kb on - strand, within MMJJ_RS06185 at 1147.535 kb on + strand, within MMJJ_RS06185 at 1147.563 kb on - strand, within MMJJ_RS06185 at 1147.591 kb on - strand, within MMJJ_RS06185 at 1147.636 kb on - strand, within MMJJ_RS06185 at 1147.636 kb on - strand, within MMJJ_RS06185 at 1147.687 kb on - strand, within MMJJ_RS06185 at 1147.739 kb on - strand, within MMJJ_RS06185 at 1147.817 kb on + strand, within MMJJ_RS06185 at 1147.829 kb on + strand, within MMJJ_RS06185 at 1148.080 kb on - strand, within MMJJ_RS06185 at 1148.165 kb on + strand at 1148.175 kb on - strand, within MMJJ_RS06185 at 1148.192 kb on + strand, within MMJJ_RS06185 at 1148.195 kb on - strand at 1148.195 kb on - strand, within MMJJ_RS06185
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,145,301 - -0.3 1,145,309 + -0.9 1,145,309 + +0.7 1,145,330 + -1.2 1,145,330 + -1.2 1,145,507 - MMJJ_RS06175 0.18 +1.6 1,145,678 + -1.0 1,145,824 - MMJJ_RS06175 0.53 -1.0 1,145,962 + MMJJ_RS06175 0.68 -0.7 1,145,962 + MMJJ_RS06175 0.68 +0.2 1,146,045 + MMJJ_RS06175 0.77 -0.6 1,146,185 + -0.3 1,146,562 - MMJJ_RS06180 0.27 -1.5 1,146,563 + +0.4 1,146,563 + MMJJ_RS06180 0.27 -2.2 1,146,564 - MMJJ_RS06180 0.27 -2.0 1,146,564 - MMJJ_RS06180 0.27 -1.0 1,146,564 - MMJJ_RS06180 0.27 -1.6 1,146,565 + +1.0 1,146,658 + MMJJ_RS06180 0.35 -0.0 1,146,697 + -1.0 1,146,842 - MMJJ_RS06180 0.53 -2.6 1,147,174 + MMJJ_RS06180 0.83 -3.1 1,147,394 + -2.3 1,147,405 + -0.9 1,147,441 - -2.0 1,147,441 - -1.6 1,147,461 - -0.8 1,147,490 + MMJJ_RS06185 0.10 -2.2 1,147,505 + MMJJ_RS06185 0.12 -0.9 1,147,532 - MMJJ_RS06185 0.15 -2.5 1,147,535 + MMJJ_RS06185 0.15 -1.0 1,147,563 - MMJJ_RS06185 0.18 -1.2 1,147,591 - MMJJ_RS06185 0.21 -0.9 1,147,636 - MMJJ_RS06185 0.25 -2.3 1,147,636 - MMJJ_RS06185 0.25 -3.0 1,147,687 - MMJJ_RS06185 0.31 -0.5 1,147,739 - MMJJ_RS06185 0.36 -2.0 1,147,817 + MMJJ_RS06185 0.44 -1.0 1,147,829 + MMJJ_RS06185 0.45 -2.2 1,148,080 - MMJJ_RS06185 0.71 -2.6 1,148,165 + -1.3 1,148,175 - MMJJ_RS06185 0.81 -1.6 1,148,192 + MMJJ_RS06185 0.83 -2.9 1,148,195 - +2.0 1,148,195 - MMJJ_RS06185 0.83 -1.1
Or see this region's nucleotide sequence