Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06025 and MMJJ_RS06030 are separated by 19 nucleotides MMJJ_RS06030 and MMJJ_RS06035 are separated by 9 nucleotides MMJJ_RS06035 and MMJJ_RS06040 are separated by 10 nucleotides MMJJ_RS06040 and MMJJ_RS06045 are separated by 39 nucleotides MMJJ_RS06045 and MMJJ_RS06050 are separated by 12 nucleotides
MMJJ_RS06025: MMJJ_RS06025 - 4Fe-4S binding protein, at 1,119,489 to 1,120,934
_RS06025
MMJJ_RS06030: MMJJ_RS06030 - 4Fe-4S binding protein, at 1,120,954 to 1,121,466
_RS06030
MMJJ_RS06035: MMJJ_RS06035 - NADH-quinone oxidoreductase subunit B family protein, at 1,121,476 to 1,121,919
_RS06035
MMJJ_RS06040: MMJJ_RS06040 - NADH-quinone oxidoreductase subunit H, at 1,121,930 to 1,122,925
_RS06040
MMJJ_RS06045: MMJJ_RS06045 - hypothetical protein, at 1,122,965 to 1,123,258
_RS06045
MMJJ_RS06050: MMJJ_RS06050 - molybdopterin molybdotransferase MoeA, at 1,123,271 to 1,124,479
_RS06050
Position (kb)
1121
1122
1123 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1120.972 kb on + strand at 1120.983 kb on - strand at 1121.035 kb on - strand, within MMJJ_RS06030 at 1121.036 kb on + strand, within MMJJ_RS06030 at 1121.085 kb on - strand, within MMJJ_RS06030 at 1121.128 kb on + strand, within MMJJ_RS06030 at 1121.230 kb on + strand, within MMJJ_RS06030 at 1121.279 kb on - strand, within MMJJ_RS06030 at 1121.279 kb on - strand, within MMJJ_RS06030 at 1121.401 kb on + strand, within MMJJ_RS06030 at 1121.405 kb on - strand, within MMJJ_RS06030 at 1121.406 kb on + strand, within MMJJ_RS06030 at 1121.406 kb on + strand, within MMJJ_RS06030 at 1121.415 kb on - strand at 1121.416 kb on + strand at 1121.464 kb on - strand at 1121.465 kb on + strand at 1121.471 kb on - strand at 1121.471 kb on - strand at 1121.471 kb on - strand at 1121.472 kb on + strand at 1121.480 kb on - strand at 1121.481 kb on + strand at 1121.481 kb on + strand at 1121.525 kb on + strand, within MMJJ_RS06035 at 1121.525 kb on + strand, within MMJJ_RS06035 at 1121.526 kb on - strand, within MMJJ_RS06035 at 1121.527 kb on + strand, within MMJJ_RS06035 at 1121.539 kb on + strand, within MMJJ_RS06035 at 1121.562 kb on + strand, within MMJJ_RS06035 at 1121.573 kb on - strand, within MMJJ_RS06035 at 1121.574 kb on + strand, within MMJJ_RS06035 at 1121.599 kb on - strand, within MMJJ_RS06035 at 1121.600 kb on + strand, within MMJJ_RS06035 at 1121.649 kb on + strand, within MMJJ_RS06035 at 1121.662 kb on + strand, within MMJJ_RS06035 at 1121.662 kb on + strand, within MMJJ_RS06035 at 1121.686 kb on + strand, within MMJJ_RS06035 at 1121.693 kb on - strand, within MMJJ_RS06035 at 1121.695 kb on - strand, within MMJJ_RS06035 at 1121.695 kb on - strand, within MMJJ_RS06035 at 1121.695 kb on - strand, within MMJJ_RS06035 at 1121.723 kb on - strand, within MMJJ_RS06035 at 1121.761 kb on - strand, within MMJJ_RS06035 at 1121.761 kb on - strand, within MMJJ_RS06035 at 1121.762 kb on + strand, within MMJJ_RS06035 at 1121.762 kb on + strand, within MMJJ_RS06035 at 1121.769 kb on - strand, within MMJJ_RS06035 at 1121.769 kb on - strand, within MMJJ_RS06035 at 1121.770 kb on + strand, within MMJJ_RS06035 at 1121.799 kb on - strand, within MMJJ_RS06035 at 1121.800 kb on + strand, within MMJJ_RS06035 at 1121.800 kb on + strand, within MMJJ_RS06035 at 1121.800 kb on + strand, within MMJJ_RS06035 at 1121.820 kb on - strand, within MMJJ_RS06035 at 1121.929 kb on - strand at 1122.014 kb on + strand at 1122.114 kb on - strand, within MMJJ_RS06040 at 1122.193 kb on + strand, within MMJJ_RS06040 at 1122.295 kb on + strand, within MMJJ_RS06040 at 1122.557 kb on - strand, within MMJJ_RS06040 at 1122.571 kb on - strand, within MMJJ_RS06040 at 1122.573 kb on - strand, within MMJJ_RS06040 at 1122.627 kb on - strand, within MMJJ_RS06040 at 1122.750 kb on - strand at 1122.817 kb on + strand, within MMJJ_RS06040 at 1122.821 kb on + strand, within MMJJ_RS06040 at 1122.828 kb on + strand at 1122.828 kb on + strand at 1122.842 kb on + strand at 1122.928 kb on + strand at 1122.928 kb on + strand at 1122.931 kb on + strand at 1122.931 kb on + strand at 1122.973 kb on - strand at 1122.974 kb on + strand at 1122.974 kb on + strand at 1123.001 kb on - strand, within MMJJ_RS06045 at 1123.001 kb on - strand, within MMJJ_RS06045 at 1123.040 kb on - strand, within MMJJ_RS06045 at 1123.040 kb on - strand, within MMJJ_RS06045 at 1123.040 kb on - strand, within MMJJ_RS06045 at 1123.040 kb on - strand, within MMJJ_RS06045 at 1123.051 kb on - strand, within MMJJ_RS06045 at 1123.051 kb on - strand, within MMJJ_RS06045 at 1123.066 kb on - strand, within MMJJ_RS06045 at 1123.067 kb on + strand, within MMJJ_RS06045 at 1123.067 kb on + strand, within MMJJ_RS06045 at 1123.068 kb on - strand, within MMJJ_RS06045 at 1123.068 kb on - strand, within MMJJ_RS06045 at 1123.140 kb on - strand, within MMJJ_RS06045 at 1123.146 kb on + strand, within MMJJ_RS06045 at 1123.148 kb on + strand, within MMJJ_RS06045 at 1123.153 kb on - strand, within MMJJ_RS06045 at 1123.153 kb on - strand, within MMJJ_RS06045 at 1123.154 kb on + strand, within MMJJ_RS06045 at 1123.160 kb on + strand, within MMJJ_RS06045 at 1123.163 kb on - strand, within MMJJ_RS06045 at 1123.163 kb on - strand, within MMJJ_RS06045 at 1123.163 kb on - strand, within MMJJ_RS06045 at 1123.164 kb on + strand, within MMJJ_RS06045 at 1123.182 kb on - strand, within MMJJ_RS06045 at 1123.183 kb on + strand, within MMJJ_RS06045 at 1123.200 kb on + strand, within MMJJ_RS06045 at 1123.204 kb on + strand, within MMJJ_RS06045 at 1123.251 kb on - strand at 1123.251 kb on - strand at 1123.251 kb on - strand at 1123.258 kb on - strand at 1123.259 kb on + strand at 1123.281 kb on - strand at 1123.281 kb on - strand at 1123.329 kb on + strand at 1123.334 kb on - strand at 1123.334 kb on - strand at 1123.338 kb on - strand at 1123.339 kb on + strand at 1123.357 kb on - strand at 1123.358 kb on + strand at 1123.358 kb on + strand at 1123.421 kb on - strand, within MMJJ_RS06050 at 1123.422 kb on + strand, within MMJJ_RS06050 at 1123.424 kb on + strand, within MMJJ_RS06050 at 1123.437 kb on + strand, within MMJJ_RS06050 at 1123.437 kb on + strand, within MMJJ_RS06050 at 1123.441 kb on - strand, within MMJJ_RS06050 at 1123.441 kb on - strand, within MMJJ_RS06050 at 1123.635 kb on + strand, within MMJJ_RS06050 at 1123.673 kb on + strand, within MMJJ_RS06050 at 1123.676 kb on - strand, within MMJJ_RS06050 at 1123.724 kb on + strand, within MMJJ_RS06050 at 1123.757 kb on - strand, within MMJJ_RS06050 at 1123.798 kb on - strand, within MMJJ_RS06050 at 1123.798 kb on - strand, within MMJJ_RS06050 at 1123.849 kb on - strand, within MMJJ_RS06050 at 1123.920 kb on + strand, within MMJJ_RS06050
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 1,120,972 + +0.5 1,120,983 - -0.4 1,121,035 - MMJJ_RS06030 0.16 -1.1 1,121,036 + MMJJ_RS06030 0.16 +0.1 1,121,085 - MMJJ_RS06030 0.26 -3.8 1,121,128 + MMJJ_RS06030 0.34 -1.0 1,121,230 + MMJJ_RS06030 0.54 +0.6 1,121,279 - MMJJ_RS06030 0.63 +0.5 1,121,279 - MMJJ_RS06030 0.63 -0.4 1,121,401 + MMJJ_RS06030 0.87 +2.4 1,121,405 - MMJJ_RS06030 0.88 +0.1 1,121,406 + MMJJ_RS06030 0.88 +0.6 1,121,406 + MMJJ_RS06030 0.88 -1.2 1,121,415 - -0.5 1,121,416 + -0.6 1,121,464 - -0.5 1,121,465 + -0.0 1,121,471 - -1.8 1,121,471 - -1.7 1,121,471 - -2.2 1,121,472 + -0.9 1,121,480 - +0.2 1,121,481 + +0.1 1,121,481 + -0.5 1,121,525 + MMJJ_RS06035 0.11 -0.4 1,121,525 + MMJJ_RS06035 0.11 -1.2 1,121,526 - MMJJ_RS06035 0.11 -1.2 1,121,527 + MMJJ_RS06035 0.11 -0.9 1,121,539 + MMJJ_RS06035 0.14 -0.4 1,121,562 + MMJJ_RS06035 0.19 -1.8 1,121,573 - MMJJ_RS06035 0.22 -0.2 1,121,574 + MMJJ_RS06035 0.22 -1.6 1,121,599 - MMJJ_RS06035 0.28 -0.3 1,121,600 + MMJJ_RS06035 0.28 -1.1 1,121,649 + MMJJ_RS06035 0.39 -1.1 1,121,662 + MMJJ_RS06035 0.42 +0.7 1,121,662 + MMJJ_RS06035 0.42 -0.6 1,121,686 + MMJJ_RS06035 0.47 -2.1 1,121,693 - MMJJ_RS06035 0.49 -0.4 1,121,695 - MMJJ_RS06035 0.49 -1.8 1,121,695 - MMJJ_RS06035 0.49 -1.7 1,121,695 - MMJJ_RS06035 0.49 -1.0 1,121,723 - MMJJ_RS06035 0.56 +0.5 1,121,761 - MMJJ_RS06035 0.64 -0.6 1,121,761 - MMJJ_RS06035 0.64 -0.8 1,121,762 + MMJJ_RS06035 0.64 +0.3 1,121,762 + MMJJ_RS06035 0.64 -0.4 1,121,769 - MMJJ_RS06035 0.66 -0.3 1,121,769 - MMJJ_RS06035 0.66 -0.2 1,121,770 + MMJJ_RS06035 0.66 -1.0 1,121,799 - MMJJ_RS06035 0.73 -1.3 1,121,800 + MMJJ_RS06035 0.73 -3.6 1,121,800 + MMJJ_RS06035 0.73 -1.3 1,121,800 + MMJJ_RS06035 0.73 +0.2 1,121,820 - MMJJ_RS06035 0.77 -0.5 1,121,929 - -2.1 1,122,014 + -1.2 1,122,114 - MMJJ_RS06040 0.18 -2.7 1,122,193 + MMJJ_RS06040 0.26 -1.6 1,122,295 + MMJJ_RS06040 0.37 -2.2 1,122,557 - MMJJ_RS06040 0.63 -1.8 1,122,571 - MMJJ_RS06040 0.64 -2.3 1,122,573 - MMJJ_RS06040 0.65 -2.7 1,122,627 - MMJJ_RS06040 0.70 -2.5 1,122,750 - -0.0 1,122,817 + MMJJ_RS06040 0.89 -2.5 1,122,821 + MMJJ_RS06040 0.89 -2.4 1,122,828 + +1.0 1,122,828 + -0.0 1,122,842 + -1.0 1,122,928 + +0.4 1,122,928 + +0.3 1,122,931 + -0.5 1,122,931 + +0.7 1,122,973 - +0.0 1,122,974 + +0.1 1,122,974 + +0.1 1,123,001 - MMJJ_RS06045 0.12 +0.3 1,123,001 - MMJJ_RS06045 0.12 +0.2 1,123,040 - MMJJ_RS06045 0.26 -1.9 1,123,040 - MMJJ_RS06045 0.26 +0.4 1,123,040 - MMJJ_RS06045 0.26 +0.4 1,123,040 - MMJJ_RS06045 0.26 +0.7 1,123,051 - MMJJ_RS06045 0.29 -0.5 1,123,051 - MMJJ_RS06045 0.29 +0.3 1,123,066 - MMJJ_RS06045 0.34 +0.9 1,123,067 + MMJJ_RS06045 0.35 +0.7 1,123,067 + MMJJ_RS06045 0.35 +0.5 1,123,068 - MMJJ_RS06045 0.35 +0.4 1,123,068 - MMJJ_RS06045 0.35 +1.3 1,123,140 - MMJJ_RS06045 0.60 -0.0 1,123,146 + MMJJ_RS06045 0.62 +0.8 1,123,148 + MMJJ_RS06045 0.62 -0.4 1,123,153 - MMJJ_RS06045 0.64 +0.1 1,123,153 - MMJJ_RS06045 0.64 +0.3 1,123,154 + MMJJ_RS06045 0.64 -0.3 1,123,160 + MMJJ_RS06045 0.66 -0.0 1,123,163 - MMJJ_RS06045 0.67 -0.2 1,123,163 - MMJJ_RS06045 0.67 +1.1 1,123,163 - MMJJ_RS06045 0.67 +0.8 1,123,164 + MMJJ_RS06045 0.68 -0.5 1,123,182 - MMJJ_RS06045 0.74 +2.5 1,123,183 + MMJJ_RS06045 0.74 -0.3 1,123,200 + MMJJ_RS06045 0.80 -2.5 1,123,204 + MMJJ_RS06045 0.81 +0.2 1,123,251 - -0.7 1,123,251 - +0.4 1,123,251 - +0.6 1,123,258 - -0.5 1,123,259 + +0.8 1,123,281 - -2.0 1,123,281 - +0.4 1,123,329 + -0.8 1,123,334 - -1.3 1,123,334 - -1.7 1,123,338 - -0.5 1,123,339 + -1.9 1,123,357 - -2.7 1,123,358 + -1.1 1,123,358 + -0.6 1,123,421 - MMJJ_RS06050 0.12 -1.1 1,123,422 + MMJJ_RS06050 0.12 -1.5 1,123,424 + MMJJ_RS06050 0.13 -1.9 1,123,437 + MMJJ_RS06050 0.14 -2.1 1,123,437 + MMJJ_RS06050 0.14 -0.9 1,123,441 - MMJJ_RS06050 0.14 -1.6 1,123,441 - MMJJ_RS06050 0.14 -2.0 1,123,635 + MMJJ_RS06050 0.30 -0.4 1,123,673 + MMJJ_RS06050 0.33 -0.8 1,123,676 - MMJJ_RS06050 0.33 +0.2 1,123,724 + MMJJ_RS06050 0.37 -2.0 1,123,757 - MMJJ_RS06050 0.40 -3.4 1,123,798 - MMJJ_RS06050 0.44 -2.3 1,123,798 - MMJJ_RS06050 0.44 +0.1 1,123,849 - MMJJ_RS06050 0.48 -2.5 1,123,920 + MMJJ_RS06050 0.54 -2.5
Or see this region's nucleotide sequence