Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05175 and MMJJ_RS05180 are separated by 1 nucleotides MMJJ_RS05180 and MMJJ_RS05185 are separated by 37 nucleotides MMJJ_RS05185 and MMJJ_RS05190 are separated by 48 nucleotides MMJJ_RS05190 and MMJJ_RS05195 are separated by 38 nucleotides MMJJ_RS05195 and MMJJ_RS05200 are separated by 57 nucleotides
MMJJ_RS05175: MMJJ_RS05175 - CBS domain-containing protein, at 957,632 to 958,543
_RS05175
MMJJ_RS05180: MMJJ_RS05180 - phosphoribosyl-ATP diphosphatase, at 958,545 to 958,835
_RS05180
MMJJ_RS05185: MMJJ_RS05185 - 6,7-dimethyl-8-ribityllumazine synthase, at 958,873 to 959,286
_RS05185
MMJJ_RS05190: MMJJ_RS05190 - gamma carbonic anhydrase family protein, at 959,335 to 959,790
_RS05190
MMJJ_RS05195: MMJJ_RS05195 - hypothetical protein, at 959,829 to 960,101
_RS05195
MMJJ_RS05200: MMJJ_RS05200 - TIGR04013 family B12-binding domain/radical SAM domain-containing protein, at 960,159 to 961,268
_RS05200
Position (kb) 959
960 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 958.345 kb on + strand, within MMJJ_RS05175 at 958.386 kb on - strand, within MMJJ_RS05175 at 958.418 kb on + strand at 958.484 kb on + strand at 958.495 kb on + strand at 958.505 kb on + strand at 958.541 kb on - strand at 958.546 kb on - strand at 958.546 kb on - strand at 958.575 kb on - strand, within MMJJ_RS05180 at 958.768 kb on - strand at 958.768 kb on - strand, within MMJJ_RS05180 at 958.769 kb on + strand, within MMJJ_RS05180 at 958.816 kb on + strand at 958.825 kb on + strand at 959.311 kb on + strand at 959.314 kb on + strand at 959.351 kb on + strand at 959.360 kb on - strand at 959.389 kb on - strand, within MMJJ_RS05190 at 959.413 kb on - strand, within MMJJ_RS05190 at 959.445 kb on - strand, within MMJJ_RS05190 at 959.524 kb on - strand, within MMJJ_RS05190 at 959.525 kb on + strand, within MMJJ_RS05190 at 959.551 kb on - strand, within MMJJ_RS05190 at 959.551 kb on - strand, within MMJJ_RS05190 at 959.680 kb on - strand, within MMJJ_RS05190 at 959.691 kb on - strand, within MMJJ_RS05190 at 959.692 kb on + strand, within MMJJ_RS05190 at 959.698 kb on + strand, within MMJJ_RS05190 at 959.711 kb on + strand, within MMJJ_RS05190 at 959.727 kb on - strand, within MMJJ_RS05190 at 959.728 kb on + strand, within MMJJ_RS05190 at 959.791 kb on - strand at 959.792 kb on + strand at 959.793 kb on - strand at 959.802 kb on - strand at 959.802 kb on - strand at 959.815 kb on - strand at 959.901 kb on - strand, within MMJJ_RS05195 at 959.901 kb on - strand, within MMJJ_RS05195 at 959.902 kb on + strand, within MMJJ_RS05195 at 959.993 kb on + strand, within MMJJ_RS05195 at 960.001 kb on - strand, within MMJJ_RS05195 at 960.001 kb on - strand, within MMJJ_RS05195 at 960.006 kb on + strand, within MMJJ_RS05195 at 960.053 kb on - strand, within MMJJ_RS05195 at 960.064 kb on - strand, within MMJJ_RS05195 at 960.077 kb on - strand at 960.077 kb on - strand at 960.079 kb on - strand at 960.079 kb on - strand at 960.080 kb on + strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.131 kb on - strand at 960.131 kb on - strand at 960.132 kb on + strand at 960.133 kb on - strand at 960.134 kb on + strand at 960.135 kb on - strand at 960.135 kb on - strand at 960.136 kb on + strand at 960.136 kb on + strand at 960.136 kb on + strand at 960.148 kb on - strand at 960.148 kb on - strand at 960.148 kb on - strand at 960.148 kb on - strand at 960.148 kb on - strand at 960.148 kb on - strand at 960.149 kb on + strand at 960.149 kb on + strand at 960.152 kb on - strand at 960.152 kb on - strand at 960.158 kb on - strand at 960.170 kb on - strand at 960.192 kb on - strand at 960.192 kb on - strand at 960.192 kb on - strand at 960.193 kb on + strand at 960.193 kb on + strand at 960.193 kb on + strand at 960.198 kb on + strand at 960.198 kb on + strand at 960.198 kb on + strand at 960.208 kb on - strand at 960.209 kb on + strand at 960.209 kb on + strand at 960.209 kb on + strand at 960.210 kb on - strand at 960.217 kb on - strand at 960.221 kb on + strand at 960.221 kb on + strand at 960.232 kb on - strand at 960.232 kb on - strand at 960.283 kb on - strand, within MMJJ_RS05200 at 960.289 kb on + strand, within MMJJ_RS05200 at 960.302 kb on - strand, within MMJJ_RS05200 at 960.302 kb on - strand, within MMJJ_RS05200 at 960.331 kb on - strand, within MMJJ_RS05200 at 960.332 kb on + strand, within MMJJ_RS05200 at 960.362 kb on - strand, within MMJJ_RS05200 at 960.362 kb on - strand, within MMJJ_RS05200 at 960.364 kb on - strand, within MMJJ_RS05200 at 960.364 kb on - strand, within MMJJ_RS05200 at 960.408 kb on + strand, within MMJJ_RS05200 at 960.433 kb on - strand, within MMJJ_RS05200 at 960.436 kb on - strand, within MMJJ_RS05200 at 960.473 kb on + strand, within MMJJ_RS05200 at 960.512 kb on - strand, within MMJJ_RS05200 at 960.579 kb on + strand, within MMJJ_RS05200 at 960.594 kb on - strand, within MMJJ_RS05200 at 960.594 kb on - strand, within MMJJ_RS05200 at 960.594 kb on - strand, within MMJJ_RS05200 at 960.594 kb on - strand, within MMJJ_RS05200 at 960.602 kb on - strand at 960.603 kb on + strand, within MMJJ_RS05200 at 960.626 kb on + strand, within MMJJ_RS05200 at 960.626 kb on + strand, within MMJJ_RS05200 at 960.626 kb on + strand, within MMJJ_RS05200 at 960.638 kb on - strand, within MMJJ_RS05200 at 960.641 kb on + strand, within MMJJ_RS05200 at 960.647 kb on - strand, within MMJJ_RS05200 at 960.647 kb on - strand, within MMJJ_RS05200 at 960.647 kb on - strand, within MMJJ_RS05200 at 960.648 kb on + strand, within MMJJ_RS05200 at 960.648 kb on + strand, within MMJJ_RS05200 at 960.654 kb on + strand, within MMJJ_RS05200 at 960.654 kb on + strand, within MMJJ_RS05200 at 960.663 kb on + strand, within MMJJ_RS05200 at 960.703 kb on - strand, within MMJJ_RS05200 at 960.723 kb on - strand, within MMJJ_RS05200 at 960.724 kb on + strand, within MMJJ_RS05200 at 960.753 kb on + strand, within MMJJ_RS05200
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 958,345 + MMJJ_RS05175 0.78 +0.2 958,386 - MMJJ_RS05175 0.83 -0.0 958,418 + -1.1 958,484 + -0.3 958,495 + +0.7 958,505 + +1.0 958,541 - -2.7 958,546 - +1.0 958,546 - +0.0 958,575 - MMJJ_RS05180 0.10 -0.1 958,768 - -2.1 958,768 - MMJJ_RS05180 0.77 -1.5 958,769 + MMJJ_RS05180 0.77 -1.7 958,816 + -2.1 958,825 + -2.9 959,311 + -2.4 959,314 + -3.2 959,351 + -1.0 959,360 - -3.2 959,389 - MMJJ_RS05190 0.12 -3.5 959,413 - MMJJ_RS05190 0.17 -3.0 959,445 - MMJJ_RS05190 0.24 -1.8 959,524 - MMJJ_RS05190 0.41 -2.7 959,525 + MMJJ_RS05190 0.42 -3.4 959,551 - MMJJ_RS05190 0.47 -2.9 959,551 - MMJJ_RS05190 0.47 -2.1 959,680 - MMJJ_RS05190 0.76 -3.3 959,691 - MMJJ_RS05190 0.78 -0.9 959,692 + MMJJ_RS05190 0.78 -1.9 959,698 + MMJJ_RS05190 0.80 -2.9 959,711 + MMJJ_RS05190 0.82 -3.3 959,727 - MMJJ_RS05190 0.86 -3.1 959,728 + MMJJ_RS05190 0.86 -2.3 959,791 - -2.8 959,792 + -2.7 959,793 - -2.3 959,802 - -4.7 959,802 - -3.2 959,815 - -2.6 959,901 - MMJJ_RS05195 0.26 -2.0 959,901 - MMJJ_RS05195 0.26 -1.7 959,902 + MMJJ_RS05195 0.27 -1.2 959,993 + MMJJ_RS05195 0.60 -1.0 960,001 - MMJJ_RS05195 0.63 -1.8 960,001 - MMJJ_RS05195 0.63 -1.5 960,006 + MMJJ_RS05195 0.65 -0.5 960,053 - MMJJ_RS05195 0.82 -1.1 960,064 - MMJJ_RS05195 0.86 -0.9 960,077 - -1.5 960,077 - -0.8 960,079 - -0.8 960,079 - -0.5 960,080 + -0.1 960,081 - -1.5 960,081 - -0.1 960,081 - -0.3 960,081 - -1.0 960,081 - -1.6 960,081 - -0.8 960,081 - -2.1 960,082 + -1.2 960,082 + -1.4 960,082 + -1.1 960,082 + -1.1 960,082 + -1.3 960,082 + -0.2 960,082 + -2.0 960,131 - -0.8 960,131 - -0.2 960,132 + -1.7 960,133 - -0.4 960,134 + -0.5 960,135 - -0.1 960,135 - -0.4 960,136 + -0.2 960,136 + +0.5 960,136 + -0.7 960,148 - -0.9 960,148 - +0.1 960,148 - -0.6 960,148 - -0.7 960,148 - -0.1 960,148 - +0.9 960,149 + -0.8 960,149 + -0.3 960,152 - -0.9 960,152 - +0.0 960,158 - -0.1 960,170 - -0.3 960,192 - +1.4 960,192 - -1.0 960,192 - -0.5 960,193 + -1.0 960,193 + -0.4 960,193 + -1.0 960,198 + +0.9 960,198 + -0.5 960,198 + -0.1 960,208 - +0.8 960,209 + +0.3 960,209 + +0.5 960,209 + +0.6 960,210 - +1.3 960,217 - +0.1 960,221 + -0.1 960,221 + -0.6 960,232 - +0.3 960,232 - +1.0 960,283 - MMJJ_RS05200 0.11 +0.6 960,289 + MMJJ_RS05200 0.12 +0.3 960,302 - MMJJ_RS05200 0.13 +0.0 960,302 - MMJJ_RS05200 0.13 +0.8 960,331 - MMJJ_RS05200 0.15 -0.0 960,332 + MMJJ_RS05200 0.16 +0.7 960,362 - MMJJ_RS05200 0.18 +0.1 960,362 - MMJJ_RS05200 0.18 +0.9 960,364 - MMJJ_RS05200 0.18 -0.2 960,364 - MMJJ_RS05200 0.18 +0.4 960,408 + MMJJ_RS05200 0.22 +0.6 960,433 - MMJJ_RS05200 0.25 -0.1 960,436 - MMJJ_RS05200 0.25 +0.2 960,473 + MMJJ_RS05200 0.28 -1.1 960,512 - MMJJ_RS05200 0.32 -0.4 960,579 + MMJJ_RS05200 0.38 +0.6 960,594 - MMJJ_RS05200 0.39 -0.7 960,594 - MMJJ_RS05200 0.39 +0.2 960,594 - MMJJ_RS05200 0.39 +0.4 960,594 - MMJJ_RS05200 0.39 -0.2 960,602 - -1.7 960,603 + MMJJ_RS05200 0.40 +0.1 960,626 + MMJJ_RS05200 0.42 -0.0 960,626 + MMJJ_RS05200 0.42 +0.3 960,626 + MMJJ_RS05200 0.42 -0.3 960,638 - MMJJ_RS05200 0.43 -0.5 960,641 + MMJJ_RS05200 0.43 +0.6 960,647 - MMJJ_RS05200 0.44 +0.6 960,647 - MMJJ_RS05200 0.44 -0.1 960,647 - MMJJ_RS05200 0.44 +0.2 960,648 + MMJJ_RS05200 0.44 +0.3 960,648 + MMJJ_RS05200 0.44 -1.1 960,654 + MMJJ_RS05200 0.45 -0.1 960,654 + MMJJ_RS05200 0.45 +0.3 960,663 + MMJJ_RS05200 0.45 -0.1 960,703 - MMJJ_RS05200 0.49 +0.0 960,723 - MMJJ_RS05200 0.51 +0.4 960,724 + MMJJ_RS05200 0.51 +0.6 960,753 + MMJJ_RS05200 0.54 +0.1
Or see this region's nucleotide sequence