Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05060 and MMJJ_RS05065 are separated by 132 nucleotides MMJJ_RS05065 and MMJJ_RS05070 are separated by 114 nucleotides
MMJJ_RS05060: MMJJ_RS05060 - argininosuccinate synthase, at 939,506 to 940,699
_RS05060
MMJJ_RS05065: MMJJ_RS05065 - (4-{4-[2-(gamma-L- glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase, at 940,832 to 941,812
_RS05065
MMJJ_RS05070: MMJJ_RS05070 - DNA primase catalytic subunit PriS, at 941,927 to 943,000
_RS05070
Position (kb) 940
941
942 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 940.703 kb on - strand at 940.745 kb on - strand at 940.773 kb on - strand at 940.776 kb on - strand at 940.784 kb on - strand at 940.788 kb on - strand at 940.789 kb on + strand at 940.790 kb on - strand at 940.803 kb on + strand at 940.803 kb on + strand at 940.830 kb on - strand at 940.834 kb on + strand at 940.973 kb on - strand, within MMJJ_RS05065 at 941.027 kb on - strand, within MMJJ_RS05065 at 941.046 kb on - strand, within MMJJ_RS05065 at 941.063 kb on + strand, within MMJJ_RS05065 at 941.213 kb on + strand, within MMJJ_RS05065 at 941.222 kb on - strand, within MMJJ_RS05065 at 941.497 kb on + strand, within MMJJ_RS05065 at 941.696 kb on - strand, within MMJJ_RS05065 at 941.744 kb on + strand at 941.823 kb on - strand at 941.824 kb on + strand at 941.824 kb on + strand at 941.824 kb on + strand at 941.832 kb on + strand at 941.858 kb on - strand at 941.858 kb on - strand at 941.859 kb on + strand at 941.864 kb on - strand at 941.864 kb on - strand at 941.865 kb on + strand at 941.865 kb on + strand at 941.870 kb on - strand at 941.871 kb on + strand at 941.871 kb on + strand at 941.874 kb on - strand at 941.874 kb on - strand at 941.890 kb on - strand at 941.891 kb on + strand at 942.158 kb on + strand, within MMJJ_RS05070 at 942.254 kb on + strand, within MMJJ_RS05070 at 942.268 kb on - strand, within MMJJ_RS05070 at 942.390 kb on - strand, within MMJJ_RS05070 at 942.390 kb on - strand, within MMJJ_RS05070 at 942.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 940,703 - -0.2 940,745 - -1.2 940,773 - -0.7 940,776 - -1.0 940,784 - +1.3 940,788 - -0.0 940,789 + -0.0 940,790 - -0.9 940,803 + -1.5 940,803 + +0.1 940,830 - -0.3 940,834 + -0.8 940,973 - MMJJ_RS05065 0.14 -2.2 941,027 - MMJJ_RS05065 0.20 -2.9 941,046 - MMJJ_RS05065 0.22 -1.0 941,063 + MMJJ_RS05065 0.24 -2.8 941,213 + MMJJ_RS05065 0.39 -2.0 941,222 - MMJJ_RS05065 0.40 -0.8 941,497 + MMJJ_RS05065 0.68 -2.2 941,696 - MMJJ_RS05065 0.88 -3.9 941,744 + -1.2 941,823 - -0.7 941,824 + +0.1 941,824 + +0.6 941,824 + -0.4 941,832 + -0.5 941,858 - +0.0 941,858 - -0.6 941,859 + +1.2 941,864 - +0.3 941,864 - +0.1 941,865 + -0.0 941,865 + +0.4 941,870 - -0.4 941,871 + +0.2 941,871 + +0.4 941,874 - +1.0 941,874 - +0.3 941,890 - -0.1 941,891 + -1.3 942,158 + MMJJ_RS05070 0.22 -1.7 942,254 + MMJJ_RS05070 0.30 -3.0 942,268 - MMJJ_RS05070 0.32 -1.2 942,390 - MMJJ_RS05070 0.43 -2.3 942,390 - MMJJ_RS05070 0.43 -0.0 942,417 - -0.7
Or see this region's nucleotide sequence