Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04990 and MMJJ_RS04995 are separated by 134 nucleotides MMJJ_RS04995 and MMJJ_RS05000 overlap by 1 nucleotides
MMJJ_RS04990: MMJJ_RS04990 - hydroxymethylglutaryl-CoA reductase (NADPH), at 926,666 to 927,892
_RS04990
MMJJ_RS04995: MMJJ_RS04995 - Tfx family DNA-binding protein, at 928,027 to 928,476
_RS04995
MMJJ_RS05000: MMJJ_RS05000 - mRNA surveillance protein pelota, at 928,476 to 929,522
_RS05000
Position (kb) 928
929 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 927.179 kb on + strand at 927.338 kb on + strand, within MMJJ_RS04990 at 927.344 kb on + strand at 927.613 kb on - strand at 927.614 kb on + strand, within MMJJ_RS04990 at 927.754 kb on + strand, within MMJJ_RS04990 at 927.971 kb on + strand at 927.975 kb on - strand at 927.975 kb on - strand at 927.976 kb on + strand at 927.976 kb on + strand at 927.993 kb on - strand at 927.997 kb on + strand at 927.997 kb on + strand at 927.997 kb on + strand at 927.997 kb on + strand at 927.999 kb on + strand at 928.007 kb on - strand at 928.007 kb on - strand at 928.008 kb on + strand at 928.027 kb on + strand at 928.027 kb on + strand at 928.055 kb on - strand at 928.108 kb on + strand, within MMJJ_RS04995 at 928.183 kb on + strand, within MMJJ_RS04995 at 928.183 kb on + strand at 928.195 kb on - strand, within MMJJ_RS04995 at 928.195 kb on - strand, within MMJJ_RS04995 at 928.195 kb on - strand, within MMJJ_RS04995 at 928.229 kb on - strand at 928.251 kb on - strand, within MMJJ_RS04995 at 928.252 kb on + strand, within MMJJ_RS04995 at 928.295 kb on - strand, within MMJJ_RS04995 at 928.302 kb on + strand, within MMJJ_RS04995 at 928.302 kb on + strand, within MMJJ_RS04995 at 928.355 kb on + strand, within MMJJ_RS04995 at 928.355 kb on + strand, within MMJJ_RS04995 at 928.398 kb on + strand, within MMJJ_RS04995 at 928.414 kb on + strand, within MMJJ_RS04995 at 928.415 kb on - strand, within MMJJ_RS04995 at 928.560 kb on + strand at 928.713 kb on + strand, within MMJJ_RS05000 at 928.769 kb on - strand, within MMJJ_RS05000 at 928.937 kb on - strand at 929.104 kb on + strand, within MMJJ_RS05000 at 929.354 kb on + strand, within MMJJ_RS05000 at 929.372 kb on + strand, within MMJJ_RS05000
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 927,179 + +0.3 927,338 + MMJJ_RS04990 0.55 +0.3 927,344 + -1.5 927,613 - -0.2 927,614 + MMJJ_RS04990 0.77 -1.7 927,754 + MMJJ_RS04990 0.89 -0.7 927,971 + -0.1 927,975 - +0.1 927,975 - -0.1 927,976 + -0.5 927,976 + +0.1 927,993 - -1.7 927,997 + -0.6 927,997 + -2.5 927,997 + -1.1 927,997 + -2.0 927,999 + -1.7 928,007 - -3.3 928,007 - -2.9 928,008 + -1.2 928,027 + -2.6 928,027 + -1.3 928,055 - -2.2 928,108 + MMJJ_RS04995 0.18 -2.8 928,183 + MMJJ_RS04995 0.35 -2.3 928,183 + -0.7 928,195 - MMJJ_RS04995 0.37 -3.7 928,195 - MMJJ_RS04995 0.37 -0.9 928,195 - MMJJ_RS04995 0.37 -1.3 928,229 - -1.5 928,251 - MMJJ_RS04995 0.50 -1.2 928,252 + MMJJ_RS04995 0.50 -1.7 928,295 - MMJJ_RS04995 0.60 -1.2 928,302 + MMJJ_RS04995 0.61 -1.2 928,302 + MMJJ_RS04995 0.61 -1.5 928,355 + MMJJ_RS04995 0.73 -1.7 928,355 + MMJJ_RS04995 0.73 -1.2 928,398 + MMJJ_RS04995 0.82 -2.7 928,414 + MMJJ_RS04995 0.86 -3.0 928,415 - MMJJ_RS04995 0.86 -2.5 928,560 + +0.0 928,713 + MMJJ_RS05000 0.23 -2.0 928,769 - MMJJ_RS05000 0.28 -0.2 928,937 - -0.7 929,104 + MMJJ_RS05000 0.60 +0.3 929,354 + MMJJ_RS05000 0.84 -1.0 929,372 + MMJJ_RS05000 0.86 -2.0
Or see this region's nucleotide sequence