Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04965 and MMJJ_RS04970 are separated by 100 nucleotides MMJJ_RS04970 and MMJJ_RS04975 are separated by 22 nucleotides MMJJ_RS04975 and MMJJ_RS04980 are separated by 15 nucleotides MMJJ_RS04980 and MMJJ_RS04985 are separated by 4 nucleotides
MMJJ_RS04965: MMJJ_RS04965 - RNA polymerase Rpb4 family protein, at 922,804 to 923,127
_RS04965
MMJJ_RS04970: MMJJ_RS04970 - DUF655 domain-containing protein, at 923,228 to 923,812
_RS04970
MMJJ_RS04975: MMJJ_RS04975 - glycosyltransferase family 4 protein, at 923,835 to 924,902
_RS04975
MMJJ_RS04980: MMJJ_RS04980 - bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase, at 924,918 to 925,523
_RS04980
MMJJ_RS04985: MMJJ_RS04985 - glutamyl-tRNA reductase, at 925,528 to 926,676
_RS04985
Position (kb) 923
924
925 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 922.943 kb on - strand, within MMJJ_RS04965 at 922.943 kb on - strand, within MMJJ_RS04965 at 922.943 kb on - strand, within MMJJ_RS04965 at 922.947 kb on - strand, within MMJJ_RS04965 at 922.947 kb on - strand, within MMJJ_RS04965 at 922.948 kb on + strand, within MMJJ_RS04965 at 922.966 kb on + strand, within MMJJ_RS04965 at 923.061 kb on + strand, within MMJJ_RS04965 at 923.065 kb on - strand, within MMJJ_RS04965 at 923.065 kb on - strand, within MMJJ_RS04965 at 923.068 kb on + strand, within MMJJ_RS04965 at 923.108 kb on - strand at 923.108 kb on - strand at 923.109 kb on + strand at 923.116 kb on + strand at 923.144 kb on - strand at 923.145 kb on + strand at 923.145 kb on + strand at 923.147 kb on - strand at 923.187 kb on + strand at 923.190 kb on - strand at 923.190 kb on - strand at 923.191 kb on + strand at 923.196 kb on - strand at 923.318 kb on + strand, within MMJJ_RS04970 at 923.429 kb on - strand, within MMJJ_RS04970 at 923.613 kb on - strand, within MMJJ_RS04970 at 923.613 kb on - strand, within MMJJ_RS04970 at 923.860 kb on + strand at 924.025 kb on - strand, within MMJJ_RS04975 at 924.112 kb on + strand, within MMJJ_RS04975 at 924.196 kb on - strand, within MMJJ_RS04975 at 924.265 kb on - strand, within MMJJ_RS04975 at 924.726 kb on - strand, within MMJJ_RS04975 at 924.875 kb on - strand at 924.875 kb on - strand at 924.877 kb on - strand at 924.877 kb on - strand at 924.878 kb on + strand at 924.878 kb on + strand at 924.897 kb on + strand at 924.897 kb on + strand at 924.951 kb on + strand at 925.011 kb on + strand, within MMJJ_RS04980 at 925.058 kb on - strand, within MMJJ_RS04980 at 925.099 kb on - strand at 925.108 kb on - strand, within MMJJ_RS04980 at 925.123 kb on - strand, within MMJJ_RS04980 at 925.123 kb on - strand, within MMJJ_RS04980 at 925.124 kb on + strand, within MMJJ_RS04980 at 925.148 kb on - strand, within MMJJ_RS04980 at 925.149 kb on + strand, within MMJJ_RS04980 at 925.152 kb on + strand, within MMJJ_RS04980 at 925.209 kb on + strand, within MMJJ_RS04980 at 925.300 kb on - strand, within MMJJ_RS04980 at 925.302 kb on - strand, within MMJJ_RS04980 at 925.303 kb on + strand, within MMJJ_RS04980 at 925.469 kb on - strand at 925.661 kb on + strand, within MMJJ_RS04985 at 925.676 kb on - strand, within MMJJ_RS04985 at 925.677 kb on + strand, within MMJJ_RS04985 at 925.696 kb on + strand, within MMJJ_RS04985 at 925.788 kb on + strand, within MMJJ_RS04985
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 922,943 - MMJJ_RS04965 0.43 +1.5 922,943 - MMJJ_RS04965 0.43 -0.5 922,943 - MMJJ_RS04965 0.43 -0.3 922,947 - MMJJ_RS04965 0.44 -0.4 922,947 - MMJJ_RS04965 0.44 -1.2 922,948 + MMJJ_RS04965 0.44 -1.0 922,966 + MMJJ_RS04965 0.50 -1.2 923,061 + MMJJ_RS04965 0.79 -0.2 923,065 - MMJJ_RS04965 0.81 +1.0 923,065 - MMJJ_RS04965 0.81 -1.3 923,068 + MMJJ_RS04965 0.81 +2.2 923,108 - +0.4 923,108 - -0.7 923,109 + -0.5 923,116 + +0.0 923,144 - +0.1 923,145 + +1.5 923,145 + -1.5 923,147 - -1.6 923,187 + -0.8 923,190 - -1.2 923,190 - -0.3 923,191 + -2.5 923,196 - -0.2 923,318 + MMJJ_RS04970 0.15 -1.1 923,429 - MMJJ_RS04970 0.34 -1.2 923,613 - MMJJ_RS04970 0.66 -1.5 923,613 - MMJJ_RS04970 0.66 -1.0 923,860 + -2.3 924,025 - MMJJ_RS04975 0.18 -0.5 924,112 + MMJJ_RS04975 0.26 -2.3 924,196 - MMJJ_RS04975 0.34 -0.2 924,265 - MMJJ_RS04975 0.40 -0.2 924,726 - MMJJ_RS04975 0.83 -3.0 924,875 - -0.2 924,875 - -1.2 924,877 - -1.2 924,877 - -0.2 924,878 + -1.2 924,878 + -1.7 924,897 + +0.8 924,897 + +0.2 924,951 + -2.7 925,011 + MMJJ_RS04980 0.15 +0.4 925,058 - MMJJ_RS04980 0.23 -1.0 925,099 - +2.6 925,108 - MMJJ_RS04980 0.31 -1.1 925,123 - MMJJ_RS04980 0.34 -0.6 925,123 - MMJJ_RS04980 0.34 -0.7 925,124 + MMJJ_RS04980 0.34 -2.5 925,148 - MMJJ_RS04980 0.38 -2.6 925,149 + MMJJ_RS04980 0.38 -1.1 925,152 + MMJJ_RS04980 0.39 -1.2 925,209 + MMJJ_RS04980 0.48 -0.0 925,300 - MMJJ_RS04980 0.63 -0.7 925,302 - MMJJ_RS04980 0.63 -2.2 925,303 + MMJJ_RS04980 0.64 -1.2 925,469 - -2.0 925,661 + MMJJ_RS04985 0.12 -0.7 925,676 - MMJJ_RS04985 0.13 -1.5 925,677 + MMJJ_RS04985 0.13 -1.9 925,696 + MMJJ_RS04985 0.15 -0.2 925,788 + MMJJ_RS04985 0.23 -2.5
Or see this region's nucleotide sequence