Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04775 and MMJJ_RS04780 are separated by 3 nucleotides MMJJ_RS04780 and MMJJ_RS04785 are separated by 12 nucleotides MMJJ_RS04785 and MMJJ_RS04790 overlap by 20 nucleotides MMJJ_RS04790 and MMJJ_RS04795 are separated by 12 nucleotides MMJJ_RS04795 and MMJJ_RS04800 are separated by 46 nucleotides
MMJJ_RS04775: MMJJ_RS04775 - DNA-binding protein, at 896,721 to 897,632
_RS04775
MMJJ_RS04780: MMJJ_RS04780 - nucleic acid-binding protein, at 897,636 to 897,953
_RS04780
MMJJ_RS04785: MMJJ_RS04785 - OB-fold nucleic acid binding domain-containing protein, at 897,966 to 898,352
_RS04785
MMJJ_RS04790: MMJJ_RS04790 - biotin--[acetyl-CoA-carboxylase] ligase, at 898,333 to 899,058
_RS04790
MMJJ_RS04795: MMJJ_RS04795 - MBL fold metallo-hydrolase, at 899,071 to 899,688
_RS04795
MMJJ_RS04800: MMJJ_RS04800 - 2-phospho-L-lactate guanylyltransferase, at 899,735 to 900,403
_RS04800
Position (kb) 898
899
900 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 897.364 kb on - strand, within MMJJ_RS04775 at 897.413 kb on + strand, within MMJJ_RS04775 at 897.413 kb on + strand, within MMJJ_RS04775 at 897.417 kb on + strand, within MMJJ_RS04775 at 897.423 kb on + strand, within MMJJ_RS04775 at 897.423 kb on + strand, within MMJJ_RS04775 at 897.518 kb on + strand, within MMJJ_RS04775 at 897.520 kb on - strand, within MMJJ_RS04775 at 897.538 kb on - strand, within MMJJ_RS04775 at 897.539 kb on + strand, within MMJJ_RS04775 at 897.540 kb on - strand, within MMJJ_RS04775 at 897.541 kb on + strand at 897.541 kb on + strand at 897.554 kb on + strand at 897.570 kb on + strand at 897.596 kb on - strand at 897.597 kb on + strand at 897.598 kb on - strand at 897.639 kb on - strand at 897.640 kb on + strand at 897.802 kb on - strand, within MMJJ_RS04780 at 897.803 kb on + strand, within MMJJ_RS04780 at 897.806 kb on - strand, within MMJJ_RS04780 at 897.807 kb on + strand, within MMJJ_RS04780 at 897.863 kb on - strand, within MMJJ_RS04780 at 897.864 kb on + strand, within MMJJ_RS04780 at 897.883 kb on - strand, within MMJJ_RS04780 at 897.884 kb on + strand, within MMJJ_RS04780 at 897.884 kb on + strand, within MMJJ_RS04780 at 898.003 kb on - strand at 898.004 kb on + strand at 898.050 kb on - strand, within MMJJ_RS04785 at 898.050 kb on - strand, within MMJJ_RS04785 at 898.050 kb on - strand, within MMJJ_RS04785 at 898.052 kb on - strand, within MMJJ_RS04785 at 898.052 kb on - strand, within MMJJ_RS04785 at 898.053 kb on + strand, within MMJJ_RS04785 at 898.053 kb on + strand, within MMJJ_RS04785 at 898.053 kb on + strand, within MMJJ_RS04785 at 898.062 kb on + strand, within MMJJ_RS04785 at 898.093 kb on - strand, within MMJJ_RS04785 at 898.103 kb on + strand, within MMJJ_RS04785 at 898.145 kb on - strand, within MMJJ_RS04785 at 898.145 kb on - strand, within MMJJ_RS04785 at 898.268 kb on + strand, within MMJJ_RS04785 at 898.277 kb on - strand, within MMJJ_RS04785 at 898.277 kb on - strand, within MMJJ_RS04785 at 898.277 kb on - strand, within MMJJ_RS04785 at 898.278 kb on + strand, within MMJJ_RS04785 at 898.281 kb on - strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.285 kb on - strand, within MMJJ_RS04785 at 898.286 kb on + strand, within MMJJ_RS04785 at 898.286 kb on + strand, within MMJJ_RS04785 at 898.330 kb on - strand at 898.336 kb on - strand at 898.336 kb on - strand at 898.337 kb on + strand at 898.337 kb on + strand at 898.337 kb on + strand at 898.351 kb on + strand at 898.351 kb on + strand at 898.411 kb on - strand, within MMJJ_RS04790 at 898.412 kb on + strand, within MMJJ_RS04790 at 898.412 kb on + strand, within MMJJ_RS04790 at 898.485 kb on - strand, within MMJJ_RS04790 at 898.602 kb on + strand, within MMJJ_RS04790 at 898.722 kb on + strand at 898.790 kb on - strand, within MMJJ_RS04790 at 898.850 kb on - strand, within MMJJ_RS04790 at 898.851 kb on + strand, within MMJJ_RS04790 at 898.924 kb on + strand at 898.931 kb on - strand, within MMJJ_RS04790 at 899.056 kb on - strand at 899.068 kb on + strand at 899.079 kb on - strand at 899.113 kb on - strand at 899.117 kb on - strand at 899.120 kb on - strand at 899.144 kb on - strand, within MMJJ_RS04795 at 899.192 kb on - strand, within MMJJ_RS04795 at 899.192 kb on - strand, within MMJJ_RS04795 at 899.193 kb on + strand, within MMJJ_RS04795 at 899.193 kb on + strand, within MMJJ_RS04795 at 899.193 kb on + strand, within MMJJ_RS04795 at 899.197 kb on - strand, within MMJJ_RS04795 at 899.198 kb on + strand, within MMJJ_RS04795 at 899.215 kb on - strand, within MMJJ_RS04795 at 899.216 kb on + strand, within MMJJ_RS04795 at 899.216 kb on + strand, within MMJJ_RS04795 at 899.216 kb on + strand, within MMJJ_RS04795 at 899.220 kb on - strand, within MMJJ_RS04795 at 899.221 kb on + strand, within MMJJ_RS04795 at 899.306 kb on - strand, within MMJJ_RS04795 at 899.307 kb on + strand, within MMJJ_RS04795 at 899.307 kb on + strand, within MMJJ_RS04795 at 899.307 kb on + strand, within MMJJ_RS04795 at 899.341 kb on + strand, within MMJJ_RS04795 at 899.341 kb on + strand, within MMJJ_RS04795 at 899.345 kb on - strand, within MMJJ_RS04795 at 899.349 kb on - strand, within MMJJ_RS04795 at 899.397 kb on + strand, within MMJJ_RS04795 at 899.397 kb on + strand, within MMJJ_RS04795 at 899.397 kb on + strand, within MMJJ_RS04795 at 899.417 kb on - strand, within MMJJ_RS04795 at 899.441 kb on - strand, within MMJJ_RS04795 at 899.441 kb on - strand, within MMJJ_RS04795 at 899.441 kb on - strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.472 kb on + strand, within MMJJ_RS04795 at 899.477 kb on - strand, within MMJJ_RS04795 at 899.478 kb on + strand, within MMJJ_RS04795 at 899.478 kb on + strand, within MMJJ_RS04795 at 899.480 kb on - strand, within MMJJ_RS04795 at 899.481 kb on + strand, within MMJJ_RS04795 at 899.604 kb on - strand, within MMJJ_RS04795 at 899.624 kb on - strand, within MMJJ_RS04795 at 899.625 kb on + strand, within MMJJ_RS04795 at 899.625 kb on + strand, within MMJJ_RS04795 at 899.626 kb on - strand, within MMJJ_RS04795 at 899.654 kb on - strand at 899.678 kb on - strand at 899.679 kb on + strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.683 kb on + strand at 899.683 kb on + strand at 899.717 kb on + strand at 899.749 kb on + strand at 899.775 kb on - strand at 899.838 kb on - strand, within MMJJ_RS04800 at 899.838 kb on - strand, within MMJJ_RS04800 at 899.838 kb on - strand, within MMJJ_RS04800 at 899.850 kb on + strand, within MMJJ_RS04800 at 899.883 kb on + strand, within MMJJ_RS04800 at 899.963 kb on + strand, within MMJJ_RS04800
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 897,364 - MMJJ_RS04775 0.71 -2.9 897,413 + MMJJ_RS04775 0.76 +0.3 897,413 + MMJJ_RS04775 0.76 -0.2 897,417 + MMJJ_RS04775 0.76 +0.0 897,423 + MMJJ_RS04775 0.77 +0.7 897,423 + MMJJ_RS04775 0.77 -0.0 897,518 + MMJJ_RS04775 0.87 -0.4 897,520 - MMJJ_RS04775 0.88 +0.5 897,538 - MMJJ_RS04775 0.90 +2.8 897,539 + MMJJ_RS04775 0.90 -0.7 897,540 - MMJJ_RS04775 0.90 -0.9 897,541 + -0.6 897,541 + +0.6 897,554 + -0.3 897,570 + -0.1 897,596 - +0.1 897,597 + +0.8 897,598 - +0.9 897,639 - +0.1 897,640 + +1.4 897,802 - MMJJ_RS04780 0.52 +2.2 897,803 + MMJJ_RS04780 0.53 -0.2 897,806 - MMJJ_RS04780 0.53 -2.0 897,807 + MMJJ_RS04780 0.54 -0.3 897,863 - MMJJ_RS04780 0.71 +0.5 897,864 + MMJJ_RS04780 0.72 +0.4 897,883 - MMJJ_RS04780 0.78 +0.3 897,884 + MMJJ_RS04780 0.78 +0.5 897,884 + MMJJ_RS04780 0.78 -0.4 898,003 - +0.1 898,004 + +0.2 898,050 - MMJJ_RS04785 0.22 -0.4 898,050 - MMJJ_RS04785 0.22 -0.3 898,050 - MMJJ_RS04785 0.22 +0.3 898,052 - MMJJ_RS04785 0.22 -0.1 898,052 - MMJJ_RS04785 0.22 +1.0 898,053 + MMJJ_RS04785 0.22 -0.1 898,053 + MMJJ_RS04785 0.22 +0.0 898,053 + MMJJ_RS04785 0.22 +0.6 898,062 + MMJJ_RS04785 0.25 -0.0 898,093 - MMJJ_RS04785 0.33 -0.1 898,103 + MMJJ_RS04785 0.35 -1.2 898,145 - MMJJ_RS04785 0.46 -1.5 898,145 - MMJJ_RS04785 0.46 -0.8 898,268 + MMJJ_RS04785 0.78 -0.6 898,277 - MMJJ_RS04785 0.80 -0.3 898,277 - MMJJ_RS04785 0.80 -1.0 898,277 - MMJJ_RS04785 0.80 +1.5 898,278 + MMJJ_RS04785 0.81 -0.7 898,281 - MMJJ_RS04785 0.81 -0.1 898,282 + MMJJ_RS04785 0.82 -0.8 898,282 + MMJJ_RS04785 0.82 -0.2 898,282 + MMJJ_RS04785 0.82 -0.4 898,282 + MMJJ_RS04785 0.82 +0.5 898,282 + MMJJ_RS04785 0.82 -0.6 898,282 + MMJJ_RS04785 0.82 +0.2 898,282 + MMJJ_RS04785 0.82 +0.2 898,285 - MMJJ_RS04785 0.82 +0.2 898,286 + MMJJ_RS04785 0.83 -0.8 898,286 + MMJJ_RS04785 0.83 +0.2 898,330 - +0.2 898,336 - -2.4 898,336 - -1.6 898,337 + -0.2 898,337 + -0.6 898,337 + -0.2 898,351 + -2.5 898,351 + -2.2 898,411 - MMJJ_RS04790 0.11 +0.6 898,412 + MMJJ_RS04790 0.11 -2.0 898,412 + MMJJ_RS04790 0.11 -2.0 898,485 - MMJJ_RS04790 0.21 -2.5 898,602 + MMJJ_RS04790 0.37 -2.9 898,722 + -1.5 898,790 - MMJJ_RS04790 0.63 -2.0 898,850 - MMJJ_RS04790 0.71 -2.8 898,851 + MMJJ_RS04790 0.71 -3.1 898,924 + -0.2 898,931 - MMJJ_RS04790 0.82 -2.0 899,056 - +0.4 899,068 + -0.5 899,079 - -0.4 899,113 - +0.0 899,117 - -0.6 899,120 - -0.1 899,144 - MMJJ_RS04795 0.12 -0.1 899,192 - MMJJ_RS04795 0.20 -1.5 899,192 - MMJJ_RS04795 0.20 -0.5 899,193 + MMJJ_RS04795 0.20 -0.5 899,193 + MMJJ_RS04795 0.20 +0.8 899,193 + MMJJ_RS04795 0.20 +0.2 899,197 - MMJJ_RS04795 0.20 +0.7 899,198 + MMJJ_RS04795 0.21 -1.0 899,215 - MMJJ_RS04795 0.23 +0.1 899,216 + MMJJ_RS04795 0.23 +0.1 899,216 + MMJJ_RS04795 0.23 -0.5 899,216 + MMJJ_RS04795 0.23 +1.4 899,220 - MMJJ_RS04795 0.24 -1.2 899,221 + MMJJ_RS04795 0.24 +0.4 899,306 - MMJJ_RS04795 0.38 -0.3 899,307 + MMJJ_RS04795 0.38 -1.8 899,307 + MMJJ_RS04795 0.38 -0.2 899,307 + MMJJ_RS04795 0.38 -0.4 899,341 + MMJJ_RS04795 0.44 -0.8 899,341 + MMJJ_RS04795 0.44 -1.8 899,345 - MMJJ_RS04795 0.44 -0.3 899,349 - MMJJ_RS04795 0.45 -0.5 899,397 + MMJJ_RS04795 0.53 +0.4 899,397 + MMJJ_RS04795 0.53 +0.4 899,397 + MMJJ_RS04795 0.53 -0.3 899,417 - MMJJ_RS04795 0.56 +0.2 899,441 - MMJJ_RS04795 0.60 -0.2 899,441 - MMJJ_RS04795 0.60 +0.5 899,441 - MMJJ_RS04795 0.60 +0.8 899,442 + MMJJ_RS04795 0.60 +0.1 899,442 + MMJJ_RS04795 0.60 +0.5 899,442 + MMJJ_RS04795 0.60 -0.5 899,442 + MMJJ_RS04795 0.60 -0.5 899,442 + MMJJ_RS04795 0.60 -0.4 899,442 + MMJJ_RS04795 0.60 +0.0 899,472 + MMJJ_RS04795 0.65 +0.3 899,477 - MMJJ_RS04795 0.66 -0.9 899,478 + MMJJ_RS04795 0.66 +0.8 899,478 + MMJJ_RS04795 0.66 +0.1 899,480 - MMJJ_RS04795 0.66 +0.1 899,481 + MMJJ_RS04795 0.66 -0.3 899,604 - MMJJ_RS04795 0.86 +0.2 899,624 - MMJJ_RS04795 0.89 -0.1 899,625 + MMJJ_RS04795 0.90 +0.2 899,625 + MMJJ_RS04795 0.90 -0.8 899,626 - MMJJ_RS04795 0.90 +0.5 899,654 - -0.3 899,678 - -0.1 899,679 + -1.1 899,682 - +0.1 899,682 - -1.2 899,682 - -0.4 899,682 - -0.2 899,682 - +0.6 899,682 - -0.8 899,683 + +0.8 899,683 + -0.8 899,717 + -1.4 899,749 + -1.7 899,775 - -0.7 899,838 - MMJJ_RS04800 0.15 -1.7 899,838 - MMJJ_RS04800 0.15 -1.4 899,838 - MMJJ_RS04800 0.15 -1.2 899,850 + MMJJ_RS04800 0.17 -1.7 899,883 + MMJJ_RS04800 0.22 -2.7 899,963 + MMJJ_RS04800 0.34 -0.2
Or see this region's nucleotide sequence