Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04650 and MMJJ_RS04655 are separated by 14 nucleotides MMJJ_RS04655 and MMJJ_RS04660 are separated by 31 nucleotides MMJJ_RS04660 and MMJJ_RS04665 are separated by 15 nucleotides
MMJJ_RS04650: MMJJ_RS04650 - nitrogenase iron protein, at 867,900 to 868,778
_RS04650
MMJJ_RS04655: MMJJ_RS04655 - FAD-dependent oxidoreductase, at 868,793 to 870,199
_RS04655
MMJJ_RS04660: MMJJ_RS04660 - hypoxanthine/guanine phosphoribosyltransferase, at 870,231 to 870,788
_RS04660
MMJJ_RS04665: MMJJ_RS04665 - fumarylacetoacetate hydrolase family protein, at 870,804 to 871,412
_RS04665
Position (kb) 868
869
870
871 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 867.797 kb on - strand at 867.798 kb on + strand at 867.802 kb on - strand at 867.802 kb on - strand at 867.802 kb on - strand at 867.803 kb on + strand at 867.805 kb on - strand at 867.805 kb on - strand at 867.808 kb on - strand at 867.812 kb on + strand at 867.812 kb on + strand at 867.812 kb on + strand at 867.816 kb on - strand at 867.816 kb on - strand at 867.817 kb on + strand at 867.817 kb on + strand at 867.817 kb on + strand at 867.817 kb on + strand at 867.817 kb on + strand at 867.818 kb on - strand at 867.819 kb on + strand at 867.819 kb on + strand at 867.819 kb on + strand at 867.821 kb on - strand at 867.821 kb on - strand at 867.821 kb on - strand at 867.826 kb on + strand at 867.835 kb on + strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.837 kb on + strand at 867.837 kb on + strand at 867.837 kb on + strand at 867.837 kb on + strand at 867.837 kb on + strand at 867.837 kb on + strand at 867.840 kb on - strand at 867.840 kb on - strand at 867.841 kb on + strand at 867.841 kb on + strand at 867.841 kb on + strand at 867.841 kb on + strand at 867.841 kb on + strand at 867.841 kb on + strand at 867.843 kb on - strand at 867.843 kb on - strand at 867.843 kb on - strand at 867.843 kb on - strand at 867.843 kb on - strand at 867.843 kb on - strand at 867.844 kb on + strand at 867.844 kb on + strand at 867.844 kb on + strand at 867.844 kb on + strand at 867.845 kb on - strand at 867.845 kb on - strand at 867.846 kb on + strand at 867.897 kb on + strand at 868.151 kb on + strand, within MMJJ_RS04650 at 868.240 kb on - strand, within MMJJ_RS04650 at 868.291 kb on - strand, within MMJJ_RS04650 at 868.292 kb on + strand, within MMJJ_RS04650 at 868.292 kb on + strand, within MMJJ_RS04650 at 868.346 kb on - strand at 868.347 kb on + strand, within MMJJ_RS04650 at 868.372 kb on + strand, within MMJJ_RS04650 at 868.378 kb on - strand, within MMJJ_RS04650 at 868.379 kb on + strand, within MMJJ_RS04650 at 868.404 kb on - strand at 868.418 kb on - strand, within MMJJ_RS04650 at 868.420 kb on - strand, within MMJJ_RS04650 at 868.421 kb on + strand, within MMJJ_RS04650 at 868.421 kb on + strand, within MMJJ_RS04650 at 868.487 kb on + strand, within MMJJ_RS04650 at 868.512 kb on + strand, within MMJJ_RS04650 at 868.517 kb on + strand, within MMJJ_RS04650 at 868.538 kb on + strand, within MMJJ_RS04650 at 868.613 kb on + strand, within MMJJ_RS04650 at 868.617 kb on - strand, within MMJJ_RS04650 at 868.646 kb on + strand, within MMJJ_RS04650 at 868.671 kb on - strand, within MMJJ_RS04650 at 868.776 kb on - strand at 868.798 kb on - strand at 868.798 kb on - strand at 868.800 kb on - strand at 868.875 kb on - strand at 868.960 kb on - strand, within MMJJ_RS04655 at 869.003 kb on - strand, within MMJJ_RS04655 at 869.003 kb on - strand, within MMJJ_RS04655 at 869.050 kb on + strand at 869.165 kb on + strand, within MMJJ_RS04655 at 869.165 kb on + strand, within MMJJ_RS04655 at 869.173 kb on - strand, within MMJJ_RS04655 at 869.225 kb on + strand, within MMJJ_RS04655 at 869.347 kb on - strand, within MMJJ_RS04655 at 869.348 kb on + strand, within MMJJ_RS04655 at 869.349 kb on - strand at 869.349 kb on - strand, within MMJJ_RS04655 at 869.351 kb on - strand, within MMJJ_RS04655 at 869.451 kb on - strand at 869.480 kb on + strand, within MMJJ_RS04655 at 869.545 kb on - strand at 869.551 kb on - strand, within MMJJ_RS04655 at 869.618 kb on + strand, within MMJJ_RS04655 at 869.653 kb on - strand, within MMJJ_RS04655 at 869.653 kb on - strand, within MMJJ_RS04655 at 869.690 kb on + strand, within MMJJ_RS04655 at 869.994 kb on + strand, within MMJJ_RS04655 at 869.994 kb on + strand, within MMJJ_RS04655 at 870.167 kb on - strand at 870.215 kb on - strand at 870.228 kb on - strand at 870.228 kb on - strand at 870.229 kb on + strand at 870.299 kb on - strand, within MMJJ_RS04660 at 870.300 kb on + strand, within MMJJ_RS04660 at 870.300 kb on + strand, within MMJJ_RS04660 at 870.310 kb on - strand, within MMJJ_RS04660 at 870.371 kb on - strand, within MMJJ_RS04660 at 870.371 kb on - strand, within MMJJ_RS04660 at 870.380 kb on + strand, within MMJJ_RS04660 at 870.380 kb on + strand, within MMJJ_RS04660 at 870.383 kb on - strand, within MMJJ_RS04660 at 870.384 kb on + strand, within MMJJ_RS04660 at 870.384 kb on + strand, within MMJJ_RS04660 at 870.400 kb on - strand, within MMJJ_RS04660 at 870.403 kb on - strand, within MMJJ_RS04660 at 870.433 kb on - strand, within MMJJ_RS04660 at 870.465 kb on - strand, within MMJJ_RS04660 at 870.466 kb on + strand, within MMJJ_RS04660 at 870.470 kb on - strand, within MMJJ_RS04660 at 870.470 kb on - strand, within MMJJ_RS04660 at 870.470 kb on - strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.489 kb on - strand, within MMJJ_RS04660 at 870.490 kb on + strand, within MMJJ_RS04660 at 870.490 kb on + strand, within MMJJ_RS04660 at 870.549 kb on - strand, within MMJJ_RS04660 at 870.549 kb on - strand, within MMJJ_RS04660 at 870.550 kb on + strand, within MMJJ_RS04660 at 870.550 kb on + strand, within MMJJ_RS04660 at 870.553 kb on - strand, within MMJJ_RS04660 at 870.566 kb on - strand, within MMJJ_RS04660 at 870.567 kb on + strand, within MMJJ_RS04660 at 870.599 kb on - strand, within MMJJ_RS04660 at 870.602 kb on + strand, within MMJJ_RS04660 at 870.631 kb on - strand, within MMJJ_RS04660 at 870.660 kb on + strand, within MMJJ_RS04660 at 870.678 kb on + strand, within MMJJ_RS04660 at 870.678 kb on + strand, within MMJJ_RS04660 at 870.733 kb on - strand at 870.741 kb on + strand at 870.741 kb on + strand at 870.746 kb on + strand at 870.746 kb on + strand at 870.746 kb on + strand at 870.781 kb on - strand at 870.781 kb on - strand at 870.782 kb on + strand at 870.830 kb on + strand at 870.847 kb on + strand at 870.911 kb on - strand, within MMJJ_RS04665 at 870.971 kb on + strand, within MMJJ_RS04665 at 871.048 kb on - strand, within MMJJ_RS04665 at 871.048 kb on - strand, within MMJJ_RS04665 at 871.049 kb on + strand, within MMJJ_RS04665 at 871.049 kb on + strand, within MMJJ_RS04665 at 871.058 kb on - strand, within MMJJ_RS04665 at 871.066 kb on - strand, within MMJJ_RS04665 at 871.134 kb on + strand, within MMJJ_RS04665 at 871.159 kb on - strand, within MMJJ_RS04665 at 871.182 kb on + strand, within MMJJ_RS04665
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 867,797 - +0.3 867,798 + -0.4 867,802 - +0.5 867,802 - +1.7 867,802 - -0.2 867,803 + -0.3 867,805 - -0.2 867,805 - +0.1 867,808 - +0.2 867,812 + -0.8 867,812 + +0.3 867,812 + +0.4 867,816 - -0.5 867,816 - +0.3 867,817 + -0.5 867,817 + -0.1 867,817 + -0.5 867,817 + +0.4 867,817 + +0.1 867,818 - +0.8 867,819 + -0.1 867,819 + -0.0 867,819 + -0.6 867,821 - -0.4 867,821 - +0.5 867,821 - -0.5 867,826 + +1.0 867,835 + +0.4 867,836 - -0.4 867,836 - -0.0 867,836 - +0.1 867,836 - -0.1 867,836 - +0.3 867,836 - -1.0 867,836 - +0.5 867,836 - -0.2 867,837 + -0.4 867,837 + -0.6 867,837 + -0.1 867,837 + -0.4 867,837 + -1.2 867,837 + +1.1 867,840 - -3.1 867,840 - -0.2 867,841 + -1.5 867,841 + -0.0 867,841 + -1.5 867,841 + -0.4 867,841 + -0.6 867,841 + +0.1 867,843 - +0.1 867,843 - +0.7 867,843 - -0.2 867,843 - -0.1 867,843 - -2.2 867,843 - -1.2 867,844 + +0.9 867,844 + +0.2 867,844 + -0.7 867,844 + +0.3 867,845 - -1.9 867,845 - -0.5 867,846 + -0.3 867,897 + -1.8 868,151 + MMJJ_RS04650 0.29 -0.8 868,240 - MMJJ_RS04650 0.39 -1.5 868,291 - MMJJ_RS04650 0.44 -2.3 868,292 + MMJJ_RS04650 0.45 -2.1 868,292 + MMJJ_RS04650 0.45 -3.0 868,346 - -1.2 868,347 + MMJJ_RS04650 0.51 -1.2 868,372 + MMJJ_RS04650 0.54 +0.2 868,378 - MMJJ_RS04650 0.54 -0.6 868,379 + MMJJ_RS04650 0.54 +0.1 868,404 - -1.7 868,418 - MMJJ_RS04650 0.59 -0.4 868,420 - MMJJ_RS04650 0.59 -3.2 868,421 + MMJJ_RS04650 0.59 -1.3 868,421 + MMJJ_RS04650 0.59 -2.7 868,487 + MMJJ_RS04650 0.67 -3.0 868,512 + MMJJ_RS04650 0.70 -0.7 868,517 + MMJJ_RS04650 0.70 -0.4 868,538 + MMJJ_RS04650 0.73 -1.3 868,613 + MMJJ_RS04650 0.81 -1.7 868,617 - MMJJ_RS04650 0.82 -0.7 868,646 + MMJJ_RS04650 0.85 -1.3 868,671 - MMJJ_RS04650 0.88 +1.6 868,776 - -2.5 868,798 - -3.7 868,798 - -1.6 868,800 - -2.0 868,875 - -1.7 868,960 - MMJJ_RS04655 0.12 -2.0 869,003 - MMJJ_RS04655 0.15 -2.8 869,003 - MMJJ_RS04655 0.15 -1.4 869,050 + -2.0 869,165 + MMJJ_RS04655 0.26 -0.7 869,165 + MMJJ_RS04655 0.26 -1.6 869,173 - MMJJ_RS04655 0.27 -2.0 869,225 + MMJJ_RS04655 0.31 -1.7 869,347 - MMJJ_RS04655 0.39 -1.3 869,348 + MMJJ_RS04655 0.39 -2.6 869,349 - -0.2 869,349 - MMJJ_RS04655 0.40 -2.7 869,351 - MMJJ_RS04655 0.40 -0.7 869,451 - +0.6 869,480 + MMJJ_RS04655 0.49 -3.2 869,545 - -2.0 869,551 - MMJJ_RS04655 0.54 -1.7 869,618 + MMJJ_RS04655 0.59 -2.3 869,653 - MMJJ_RS04655 0.61 -2.5 869,653 - MMJJ_RS04655 0.61 -0.6 869,690 + MMJJ_RS04655 0.64 -3.0 869,994 + MMJJ_RS04655 0.85 -3.0 869,994 + MMJJ_RS04655 0.85 +0.6 870,167 - +0.2 870,215 - -0.4 870,228 - +1.7 870,228 - +0.8 870,229 + -0.4 870,299 - MMJJ_RS04660 0.12 -0.7 870,300 + MMJJ_RS04660 0.12 +0.5 870,300 + MMJJ_RS04660 0.12 +0.5 870,310 - MMJJ_RS04660 0.14 +1.1 870,371 - MMJJ_RS04660 0.25 +0.3 870,371 - MMJJ_RS04660 0.25 +0.4 870,380 + MMJJ_RS04660 0.27 -1.2 870,380 + MMJJ_RS04660 0.27 -0.4 870,383 - MMJJ_RS04660 0.27 +0.3 870,384 + MMJJ_RS04660 0.27 +1.2 870,384 + MMJJ_RS04660 0.27 -0.5 870,400 - MMJJ_RS04660 0.30 -0.5 870,403 - MMJJ_RS04660 0.31 +0.5 870,433 - MMJJ_RS04660 0.36 +0.3 870,465 - MMJJ_RS04660 0.42 -0.3 870,466 + MMJJ_RS04660 0.42 +0.4 870,470 - MMJJ_RS04660 0.43 +1.1 870,470 - MMJJ_RS04660 0.43 +0.2 870,470 - MMJJ_RS04660 0.43 -1.3 870,471 + MMJJ_RS04660 0.43 +0.4 870,471 + MMJJ_RS04660 0.43 +0.2 870,471 + MMJJ_RS04660 0.43 -0.9 870,471 + MMJJ_RS04660 0.43 -2.9 870,489 - MMJJ_RS04660 0.46 -1.3 870,490 + MMJJ_RS04660 0.46 -1.1 870,490 + MMJJ_RS04660 0.46 -0.4 870,549 - MMJJ_RS04660 0.57 -1.1 870,549 - MMJJ_RS04660 0.57 +0.6 870,550 + MMJJ_RS04660 0.57 +0.8 870,550 + MMJJ_RS04660 0.57 +0.9 870,553 - MMJJ_RS04660 0.58 -0.2 870,566 - MMJJ_RS04660 0.60 +0.4 870,567 + MMJJ_RS04660 0.60 +0.3 870,599 - MMJJ_RS04660 0.66 -0.9 870,602 + MMJJ_RS04660 0.66 -0.5 870,631 - MMJJ_RS04660 0.72 -1.2 870,660 + MMJJ_RS04660 0.77 +0.3 870,678 + MMJJ_RS04660 0.80 -1.4 870,678 + MMJJ_RS04660 0.80 +0.5 870,733 - -0.0 870,741 + -0.5 870,741 + +0.4 870,746 + +0.2 870,746 + -0.8 870,746 + +0.1 870,781 - -1.2 870,781 - -0.2 870,782 + -0.4 870,830 + -1.0 870,847 + -0.2 870,911 - MMJJ_RS04665 0.18 -0.8 870,971 + MMJJ_RS04665 0.27 -1.4 871,048 - MMJJ_RS04665 0.40 -0.8 871,048 - MMJJ_RS04665 0.40 +0.7 871,049 + MMJJ_RS04665 0.40 -0.1 871,049 + MMJJ_RS04665 0.40 +1.2 871,058 - MMJJ_RS04665 0.42 +0.8 871,066 - MMJJ_RS04665 0.43 -0.0 871,134 + MMJJ_RS04665 0.54 -0.5 871,159 - MMJJ_RS04665 0.58 +0.8 871,182 + MMJJ_RS04665 0.62 -0.9
Or see this region's nucleotide sequence