Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04615 and MMJJ_RS04620 are separated by 49 nucleotides MMJJ_RS04620 and MMJJ_RS09305 are separated by 25 nucleotides MMJJ_RS09305 and MMJJ_RS04625 are separated by 266 nucleotides
MMJJ_RS04615: MMJJ_RS04615 - methanogenesis marker 3 protein, at 858,983 to 860,488
_RS04615
MMJJ_RS04620: MMJJ_RS04620 - homocitrate synthase family protein, at 860,538 to 861,698
_RS04620
MMJJ_RS09305: MMJJ_RS09305 - hypothetical protein, at 861,724 to 861,882
_RS09305
MMJJ_RS04625: MMJJ_RS04625 - SLC13 family permease, at 862,149 to 863,489
_RS04625
Position (kb) 860
861
862 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 859.784 kb on - strand, within MMJJ_RS04615 at 859.789 kb on + strand, within MMJJ_RS04615 at 859.789 kb on + strand, within MMJJ_RS04615 at 859.789 kb on + strand, within MMJJ_RS04615 at 859.789 kb on + strand, within MMJJ_RS04615 at 860.030 kb on - strand, within MMJJ_RS04615 at 860.065 kb on - strand, within MMJJ_RS04615 at 860.129 kb on + strand, within MMJJ_RS04615 at 860.138 kb on - strand at 860.185 kb on + strand, within MMJJ_RS04615 at 860.212 kb on + strand, within MMJJ_RS04615 at 860.461 kb on + strand at 860.462 kb on - strand at 860.462 kb on - strand at 860.463 kb on + strand at 860.463 kb on + strand at 860.466 kb on - strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.514 kb on - strand at 860.515 kb on + strand at 860.519 kb on - strand at 860.521 kb on - strand at 860.521 kb on - strand at 860.603 kb on - strand at 860.856 kb on + strand, within MMJJ_RS04620 at 860.897 kb on + strand, within MMJJ_RS04620 at 860.901 kb on + strand, within MMJJ_RS04620 at 861.074 kb on - strand, within MMJJ_RS04620 at 861.112 kb on - strand at 861.113 kb on + strand at 861.124 kb on - strand, within MMJJ_RS04620 at 861.668 kb on - strand at 861.697 kb on + strand at 861.697 kb on + strand at 861.701 kb on - strand at 861.723 kb on - strand at 861.724 kb on + strand at 861.735 kb on - strand at 861.736 kb on + strand at 861.746 kb on - strand, within MMJJ_RS09305 at 861.746 kb on - strand, within MMJJ_RS09305 at 861.746 kb on - strand, within MMJJ_RS09305 at 861.746 kb on - strand, within MMJJ_RS09305 at 861.757 kb on + strand, within MMJJ_RS09305 at 861.757 kb on + strand, within MMJJ_RS09305 at 861.798 kb on - strand, within MMJJ_RS09305 at 861.810 kb on - strand, within MMJJ_RS09305 at 861.811 kb on + strand, within MMJJ_RS09305 at 861.811 kb on + strand, within MMJJ_RS09305 at 861.811 kb on + strand, within MMJJ_RS09305 at 861.818 kb on - strand, within MMJJ_RS09305 at 861.824 kb on - strand, within MMJJ_RS09305 at 861.825 kb on + strand, within MMJJ_RS09305 at 861.825 kb on + strand, within MMJJ_RS09305 at 861.827 kb on - strand, within MMJJ_RS09305 at 861.846 kb on + strand, within MMJJ_RS09305 at 861.851 kb on - strand, within MMJJ_RS09305 at 861.859 kb on - strand, within MMJJ_RS09305 at 861.860 kb on + strand, within MMJJ_RS09305 at 861.872 kb on - strand at 861.876 kb on + strand at 861.881 kb on + strand at 861.939 kb on + strand at 861.939 kb on + strand at 861.955 kb on - strand at 861.956 kb on + strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.965 kb on + strand at 861.965 kb on + strand at 861.965 kb on + strand at 861.965 kb on + strand at 861.965 kb on + strand at 861.968 kb on - strand at 861.968 kb on - strand at 861.968 kb on - strand at 861.968 kb on - strand at 861.968 kb on - strand at 861.969 kb on + strand at 861.969 kb on + strand at 861.996 kb on - strand at 861.996 kb on - strand at 861.997 kb on + strand at 861.997 kb on + strand at 861.997 kb on + strand at 861.997 kb on + strand at 862.000 kb on - strand at 862.000 kb on - strand at 862.000 kb on - strand at 862.000 kb on - strand at 862.001 kb on + strand at 862.001 kb on + strand at 862.001 kb on + strand at 862.042 kb on - strand at 862.042 kb on - strand at 862.042 kb on - strand at 862.042 kb on - strand at 862.042 kb on - strand at 862.042 kb on - strand at 862.043 kb on + strand at 862.043 kb on + strand at 862.049 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.056 kb on + strand at 862.056 kb on + strand at 862.056 kb on + strand at 862.056 kb on + strand at 862.056 kb on + strand at 862.058 kb on - strand at 862.059 kb on + strand at 862.062 kb on - strand at 862.150 kb on - strand at 862.189 kb on + strand at 862.235 kb on - strand at 862.260 kb on - strand at 862.261 kb on + strand at 862.261 kb on + strand at 862.293 kb on - strand, within MMJJ_RS04625 at 862.480 kb on - strand, within MMJJ_RS04625 at 862.481 kb on + strand, within MMJJ_RS04625 at 862.481 kb on + strand, within MMJJ_RS04625 at 862.508 kb on - strand, within MMJJ_RS04625 at 862.509 kb on + strand, within MMJJ_RS04625 at 862.623 kb on - strand, within MMJJ_RS04625 at 862.623 kb on - strand, within MMJJ_RS04625 at 862.623 kb on - strand, within MMJJ_RS04625 at 862.632 kb on + strand, within MMJJ_RS04625 at 862.632 kb on + strand, within MMJJ_RS04625 at 862.657 kb on + strand, within MMJJ_RS04625 at 862.668 kb on + strand, within MMJJ_RS04625 at 862.686 kb on - strand, within MMJJ_RS04625 at 862.689 kb on - strand, within MMJJ_RS04625 at 862.690 kb on + strand, within MMJJ_RS04625
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 859,784 - MMJJ_RS04615 0.53 +1.2 859,789 + MMJJ_RS04615 0.54 -3.1 859,789 + MMJJ_RS04615 0.54 -2.2 859,789 + MMJJ_RS04615 0.54 -2.2 859,789 + MMJJ_RS04615 0.54 -1.5 860,030 - MMJJ_RS04615 0.70 -0.5 860,065 - MMJJ_RS04615 0.72 -3.2 860,129 + MMJJ_RS04615 0.76 -1.9 860,138 - -1.2 860,185 + MMJJ_RS04615 0.80 -0.6 860,212 + MMJJ_RS04615 0.82 -1.7 860,461 + -1.5 860,462 - -0.7 860,462 - -2.5 860,463 + +0.6 860,463 + -0.2 860,466 - -1.5 860,467 + +0.8 860,467 + -2.0 860,467 + +0.3 860,467 + -1.7 860,467 + -0.7 860,467 + +0.3 860,467 + -0.3 860,514 - -2.2 860,515 + -1.7 860,519 - -1.2 860,521 - -3.7 860,521 - -1.7 860,603 - -2.6 860,856 + MMJJ_RS04620 0.27 -2.2 860,897 + MMJJ_RS04620 0.31 -2.2 860,901 + MMJJ_RS04620 0.31 -0.8 861,074 - MMJJ_RS04620 0.46 -2.0 861,112 - -0.2 861,113 + -1.2 861,124 - MMJJ_RS04620 0.50 -2.2 861,668 - -1.5 861,697 + -2.0 861,697 + -1.5 861,701 - -1.2 861,723 - -0.5 861,724 + -0.8 861,735 - +0.7 861,736 + +0.3 861,746 - MMJJ_RS09305 0.14 +0.8 861,746 - MMJJ_RS09305 0.14 +0.1 861,746 - MMJJ_RS09305 0.14 +0.8 861,746 - MMJJ_RS09305 0.14 +0.5 861,757 + MMJJ_RS09305 0.21 -0.6 861,757 + MMJJ_RS09305 0.21 +0.4 861,798 - MMJJ_RS09305 0.47 -0.1 861,810 - MMJJ_RS09305 0.54 -0.3 861,811 + MMJJ_RS09305 0.55 +0.1 861,811 + MMJJ_RS09305 0.55 -0.7 861,811 + MMJJ_RS09305 0.55 +0.3 861,818 - MMJJ_RS09305 0.59 -0.3 861,824 - MMJJ_RS09305 0.63 +0.5 861,825 + MMJJ_RS09305 0.64 +0.2 861,825 + MMJJ_RS09305 0.64 -1.1 861,827 - MMJJ_RS09305 0.65 -0.6 861,846 + MMJJ_RS09305 0.77 -0.0 861,851 - MMJJ_RS09305 0.80 +0.3 861,859 - MMJJ_RS09305 0.85 +0.7 861,860 + MMJJ_RS09305 0.86 -0.1 861,872 - +0.3 861,876 + -0.6 861,881 + +1.1 861,939 + +0.7 861,939 + +0.1 861,955 - +0.4 861,956 + +0.2 861,964 - +0.1 861,964 - -0.3 861,964 - -0.4 861,964 - +0.0 861,964 - +0.8 861,964 - +0.4 861,964 - -0.1 861,964 - +0.8 861,964 - -0.1 861,965 + -2.5 861,965 + +0.8 861,965 + +0.3 861,965 + -0.0 861,965 + +0.3 861,968 - -0.7 861,968 - +0.4 861,968 - +0.2 861,968 - -0.2 861,968 - +1.2 861,969 + +0.3 861,969 + -0.4 861,996 - +0.2 861,996 - -0.3 861,997 + -0.6 861,997 + -0.6 861,997 + +1.0 861,997 + -0.1 862,000 - +0.1 862,000 - +0.6 862,000 - -0.5 862,000 - -1.1 862,001 + +1.0 862,001 + +0.2 862,001 + +0.8 862,042 - -0.3 862,042 - -0.5 862,042 - -0.4 862,042 - +0.8 862,042 - +0.2 862,042 - -0.5 862,043 + +1.2 862,043 + -1.2 862,049 - -0.8 862,053 - +0.1 862,053 - -0.4 862,053 - +0.5 862,053 - +0.6 862,053 - -0.4 862,053 - -0.0 862,053 - +0.2 862,053 - -0.3 862,053 - -0.6 862,053 - -1.2 862,053 - -0.3 862,053 - -0.5 862,053 - -1.7 862,054 + +0.6 862,054 + -0.5 862,054 + +0.5 862,054 + -0.2 862,054 + +0.1 862,054 + -0.6 862,054 + +0.2 862,054 + -1.0 862,054 + +0.6 862,054 + +0.0 862,054 + +1.1 862,054 + +0.0 862,054 + +0.7 862,054 + +1.5 862,055 - -2.0 862,055 - -0.1 862,055 - -0.8 862,055 - +0.1 862,055 - +0.4 862,055 - -1.5 862,055 - +0.6 862,055 - +0.6 862,055 - +0.1 862,055 - -0.7 862,055 - +0.0 862,055 - +0.2 862,055 - +0.9 862,055 - -2.6 862,055 - +1.8 862,056 + +0.1 862,056 + +0.3 862,056 + -0.8 862,056 + +0.5 862,056 + +1.0 862,058 - +0.5 862,059 + +0.1 862,062 - +0.7 862,150 - -0.1 862,189 + +0.5 862,235 - -1.5 862,260 - -0.3 862,261 + +0.3 862,261 + -1.0 862,293 - MMJJ_RS04625 0.11 +0.9 862,480 - MMJJ_RS04625 0.25 +1.0 862,481 + MMJJ_RS04625 0.25 -0.3 862,481 + MMJJ_RS04625 0.25 +0.0 862,508 - MMJJ_RS04625 0.27 -0.3 862,509 + MMJJ_RS04625 0.27 -0.8 862,623 - MMJJ_RS04625 0.35 +0.7 862,623 - MMJJ_RS04625 0.35 +0.2 862,623 - MMJJ_RS04625 0.35 +0.3 862,632 + MMJJ_RS04625 0.36 -1.7 862,632 + MMJJ_RS04625 0.36 -2.9 862,657 + MMJJ_RS04625 0.38 +0.8 862,668 + MMJJ_RS04625 0.39 -0.3 862,686 - MMJJ_RS04625 0.40 +0.6 862,689 - MMJJ_RS04625 0.40 -0.0 862,690 + MMJJ_RS04625 0.40 -0.9
Or see this region's nucleotide sequence