Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04410 and MMJJ_RS04415 are separated by 126 nucleotides MMJJ_RS04415 and MMJJ_RS04420 are separated by 569 nucleotides MMJJ_RS04420 and MMJJ_RS04425 overlap by 11 nucleotides MMJJ_RS04425 and MMJJ_RS04430 are separated by 111 nucleotides
MMJJ_RS04410: MMJJ_RS04410 - hypothetical protein, at 823,819 to 824,229
_RS04410
MMJJ_RS04415: MMJJ_RS04415 - hypothetical protein, at 824,356 to 824,550
_RS04415
MMJJ_RS04420: MMJJ_RS04420 - type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family, at 825,120 to 825,407
_RS04420
MMJJ_RS04425: MMJJ_RS04425 - hypothetical protein, at 825,397 to 825,627
_RS04425
MMJJ_RS04430: MMJJ_RS04430 - purine NTPase, at 825,739 to 826,431
_RS04430
Position (kb) 825
826 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 824.120 kb on - strand, within MMJJ_RS04410 at 824.121 kb on + strand, within MMJJ_RS04410 at 824.278 kb on - strand at 824.279 kb on + strand at 824.294 kb on - strand at 824.294 kb on - strand at 824.295 kb on + strand at 824.296 kb on - strand at 824.296 kb on - strand at 824.301 kb on - strand at 824.333 kb on - strand at 824.333 kb on - strand at 824.334 kb on + strand at 824.403 kb on + strand, within MMJJ_RS04415 at 824.445 kb on - strand, within MMJJ_RS04415 at 824.497 kb on + strand, within MMJJ_RS04415 at 824.497 kb on + strand, within MMJJ_RS04415 at 824.578 kb on - strand at 824.646 kb on - strand at 824.698 kb on + strand at 824.754 kb on - strand at 824.754 kb on - strand at 824.755 kb on + strand at 824.755 kb on + strand at 824.757 kb on - strand at 824.757 kb on - strand at 824.757 kb on - strand at 824.758 kb on + strand at 824.758 kb on + strand at 824.764 kb on - strand at 824.764 kb on - strand at 824.765 kb on + strand at 824.765 kb on + strand at 824.786 kb on + strand at 824.786 kb on + strand at 824.786 kb on + strand at 824.788 kb on - strand at 824.788 kb on - strand at 824.788 kb on - strand at 824.788 kb on - strand at 824.789 kb on + strand at 824.789 kb on + strand at 824.789 kb on + strand at 824.808 kb on - strand at 824.823 kb on + strand at 824.825 kb on - strand at 824.844 kb on + strand at 824.851 kb on - strand at 824.852 kb on + strand at 824.857 kb on - strand at 824.857 kb on - strand at 824.857 kb on - strand at 824.857 kb on - strand at 824.857 kb on - strand at 824.858 kb on + strand at 824.858 kb on + strand at 824.858 kb on + strand at 824.858 kb on + strand at 824.858 kb on + strand at 824.859 kb on - strand at 824.859 kb on - strand at 824.860 kb on + strand at 824.860 kb on + strand at 824.860 kb on + strand at 824.860 kb on + strand at 824.893 kb on + strand at 824.899 kb on + strand at 824.899 kb on + strand at 824.899 kb on + strand at 824.998 kb on - strand at 825.017 kb on + strand at 825.028 kb on - strand at 825.045 kb on - strand at 825.086 kb on - strand at 825.121 kb on - strand at 825.148 kb on + strand at 825.154 kb on - strand, within MMJJ_RS04420 at 825.154 kb on - strand, within MMJJ_RS04420 at 825.154 kb on - strand, within MMJJ_RS04420 at 825.155 kb on + strand, within MMJJ_RS04420 at 825.155 kb on + strand, within MMJJ_RS04420 at 825.155 kb on + strand, within MMJJ_RS04420 at 825.155 kb on + strand, within MMJJ_RS04420 at 825.156 kb on - strand, within MMJJ_RS04420 at 825.156 kb on - strand, within MMJJ_RS04420 at 825.156 kb on - strand, within MMJJ_RS04420 at 825.157 kb on + strand, within MMJJ_RS04420 at 825.157 kb on + strand, within MMJJ_RS04420 at 825.157 kb on + strand, within MMJJ_RS04420 at 825.182 kb on - strand, within MMJJ_RS04420 at 825.183 kb on + strand, within MMJJ_RS04420 at 825.183 kb on + strand, within MMJJ_RS04420 at 825.186 kb on - strand, within MMJJ_RS04420 at 825.187 kb on + strand, within MMJJ_RS04420 at 825.187 kb on + strand, within MMJJ_RS04420 at 825.187 kb on + strand, within MMJJ_RS04420 at 825.187 kb on + strand, within MMJJ_RS04420 at 825.189 kb on - strand, within MMJJ_RS04420 at 825.190 kb on + strand, within MMJJ_RS04420 at 825.192 kb on + strand, within MMJJ_RS04420 at 825.194 kb on - strand, within MMJJ_RS04420 at 825.194 kb on - strand, within MMJJ_RS04420 at 825.210 kb on + strand, within MMJJ_RS04420 at 825.212 kb on + strand, within MMJJ_RS04420 at 825.270 kb on + strand, within MMJJ_RS04420 at 825.270 kb on + strand, within MMJJ_RS04420 at 825.270 kb on + strand, within MMJJ_RS04420 at 825.281 kb on + strand, within MMJJ_RS04420 at 825.287 kb on - strand, within MMJJ_RS04420 at 825.300 kb on - strand, within MMJJ_RS04420 at 825.308 kb on - strand, within MMJJ_RS04420 at 825.317 kb on - strand, within MMJJ_RS04420 at 825.342 kb on + strand, within MMJJ_RS04420 at 825.372 kb on + strand, within MMJJ_RS04420 at 825.373 kb on - strand, within MMJJ_RS04420 at 825.373 kb on - strand, within MMJJ_RS04420 at 825.374 kb on + strand, within MMJJ_RS04420 at 825.374 kb on + strand, within MMJJ_RS04420 at 825.374 kb on + strand, within MMJJ_RS04420 at 825.374 kb on + strand, within MMJJ_RS04420 at 825.398 kb on - strand at 825.398 kb on - strand at 825.400 kb on - strand at 825.400 kb on - strand at 825.400 kb on - strand at 825.400 kb on - strand at 825.400 kb on - strand at 825.400 kb on - strand at 825.401 kb on + strand at 825.401 kb on + strand at 825.481 kb on + strand, within MMJJ_RS04425 at 825.648 kb on - strand at 825.648 kb on - strand at 825.650 kb on - strand at 825.650 kb on - strand at 825.652 kb on - strand at 825.652 kb on - strand at 825.653 kb on + strand at 825.658 kb on - strand at 825.662 kb on - strand at 825.669 kb on + strand at 825.672 kb on - strand at 825.672 kb on - strand at 825.713 kb on - strand at 825.714 kb on + strand at 825.817 kb on - strand, within MMJJ_RS04430 at 825.817 kb on - strand, within MMJJ_RS04430 at 825.818 kb on + strand, within MMJJ_RS04430 at 825.822 kb on - strand, within MMJJ_RS04430 at 825.822 kb on - strand, within MMJJ_RS04430 at 825.823 kb on + strand, within MMJJ_RS04430 at 825.823 kb on + strand, within MMJJ_RS04430 at 825.827 kb on - strand, within MMJJ_RS04430 at 825.828 kb on + strand, within MMJJ_RS04430 at 825.828 kb on + strand, within MMJJ_RS04430 at 825.828 kb on + strand, within MMJJ_RS04430 at 825.828 kb on + strand, within MMJJ_RS04430 at 825.828 kb on + strand, within MMJJ_RS04430 at 825.828 kb on + strand, within MMJJ_RS04430 at 825.829 kb on - strand, within MMJJ_RS04430 at 825.830 kb on + strand, within MMJJ_RS04430 at 825.830 kb on + strand, within MMJJ_RS04430 at 825.892 kb on - strand, within MMJJ_RS04430 at 825.892 kb on - strand, within MMJJ_RS04430 at 825.897 kb on - strand, within MMJJ_RS04430 at 825.898 kb on + strand, within MMJJ_RS04430 at 825.901 kb on - strand, within MMJJ_RS04430 at 825.902 kb on + strand, within MMJJ_RS04430 at 825.902 kb on + strand, within MMJJ_RS04430 at 825.904 kb on - strand, within MMJJ_RS04430 at 825.905 kb on + strand, within MMJJ_RS04430 at 825.942 kb on - strand, within MMJJ_RS04430 at 825.955 kb on + strand, within MMJJ_RS04430 at 825.957 kb on - strand, within MMJJ_RS04430 at 825.958 kb on + strand, within MMJJ_RS04430 at 825.958 kb on + strand, within MMJJ_RS04430 at 825.962 kb on - strand, within MMJJ_RS04430 at 825.962 kb on - strand, within MMJJ_RS04430 at 825.962 kb on - strand, within MMJJ_RS04430 at 825.962 kb on - strand, within MMJJ_RS04430 at 825.995 kb on + strand, within MMJJ_RS04430 at 825.995 kb on + strand, within MMJJ_RS04430 at 826.083 kb on - strand, within MMJJ_RS04430 at 826.083 kb on - strand, within MMJJ_RS04430 at 826.084 kb on + strand, within MMJJ_RS04430 at 826.087 kb on - strand, within MMJJ_RS04430 at 826.097 kb on + strand, within MMJJ_RS04430 at 826.171 kb on + strand, within MMJJ_RS04430 at 826.172 kb on - strand, within MMJJ_RS04430 at 826.173 kb on + strand, within MMJJ_RS04430 at 826.180 kb on - strand, within MMJJ_RS04430 at 826.190 kb on + strand, within MMJJ_RS04430 at 826.222 kb on + strand, within MMJJ_RS04430 at 826.227 kb on - strand, within MMJJ_RS04430 at 826.227 kb on - strand, within MMJJ_RS04430 at 826.227 kb on - strand, within MMJJ_RS04430 at 826.228 kb on + strand, within MMJJ_RS04430 at 826.228 kb on + strand, within MMJJ_RS04430 at 826.245 kb on + strand, within MMJJ_RS04430
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 824,120 - MMJJ_RS04410 0.73 -0.5 824,121 + MMJJ_RS04410 0.73 +0.1 824,278 - -0.4 824,279 + +1.3 824,294 - +0.2 824,294 - +0.6 824,295 + +1.8 824,296 - +1.5 824,296 - +1.8 824,301 - +0.5 824,333 - +2.1 824,333 - +2.5 824,334 + +3.2 824,403 + MMJJ_RS04415 0.24 +2.9 824,445 - MMJJ_RS04415 0.46 +3.0 824,497 + MMJJ_RS04415 0.72 +2.1 824,497 + MMJJ_RS04415 0.72 +3.0 824,578 - -0.6 824,646 - +0.8 824,698 + -0.9 824,754 - +0.1 824,754 - +0.4 824,755 + +0.9 824,755 + -0.3 824,757 - -0.3 824,757 - +1.2 824,757 - +0.2 824,758 + +1.0 824,758 + -0.1 824,764 - -0.6 824,764 - +0.8 824,765 + +0.3 824,765 + -0.4 824,786 + +0.6 824,786 + +1.2 824,786 + +0.6 824,788 - -0.2 824,788 - -0.8 824,788 - +0.2 824,788 - -0.1 824,789 + +0.5 824,789 + +0.9 824,789 + -0.8 824,808 - +0.4 824,823 + +0.8 824,825 - +0.4 824,844 + -0.7 824,851 - -1.2 824,852 + -0.9 824,857 - -0.1 824,857 - -0.1 824,857 - -0.2 824,857 - -0.5 824,857 - +0.5 824,858 + -0.2 824,858 + +0.7 824,858 + +0.0 824,858 + +1.7 824,858 + -0.6 824,859 - -2.3 824,859 - +1.2 824,860 + +0.3 824,860 + -0.9 824,860 + -0.9 824,860 + +0.5 824,893 + +0.5 824,899 + -0.7 824,899 + -0.7 824,899 + +0.8 824,998 - +0.7 825,017 + +0.5 825,028 - +0.0 825,045 - +0.3 825,086 - -0.7 825,121 - +0.9 825,148 + +0.2 825,154 - MMJJ_RS04420 0.12 -0.9 825,154 - MMJJ_RS04420 0.12 +0.2 825,154 - MMJJ_RS04420 0.12 +0.4 825,155 + MMJJ_RS04420 0.12 -0.1 825,155 + MMJJ_RS04420 0.12 +0.6 825,155 + MMJJ_RS04420 0.12 +0.9 825,155 + MMJJ_RS04420 0.12 -0.7 825,156 - MMJJ_RS04420 0.12 +0.7 825,156 - MMJJ_RS04420 0.12 +0.1 825,156 - MMJJ_RS04420 0.12 +0.2 825,157 + MMJJ_RS04420 0.13 +0.2 825,157 + MMJJ_RS04420 0.13 +0.3 825,157 + MMJJ_RS04420 0.13 +0.7 825,182 - MMJJ_RS04420 0.22 +0.1 825,183 + MMJJ_RS04420 0.22 -0.2 825,183 + MMJJ_RS04420 0.22 -0.5 825,186 - MMJJ_RS04420 0.23 +0.5 825,187 + MMJJ_RS04420 0.23 +0.4 825,187 + MMJJ_RS04420 0.23 +0.8 825,187 + MMJJ_RS04420 0.23 +0.2 825,187 + MMJJ_RS04420 0.23 -0.1 825,189 - MMJJ_RS04420 0.24 +0.8 825,190 + MMJJ_RS04420 0.24 -0.5 825,192 + MMJJ_RS04420 0.25 +0.5 825,194 - MMJJ_RS04420 0.26 +0.4 825,194 - MMJJ_RS04420 0.26 -0.4 825,210 + MMJJ_RS04420 0.31 +0.1 825,212 + MMJJ_RS04420 0.32 +1.5 825,270 + MMJJ_RS04420 0.52 +0.2 825,270 + MMJJ_RS04420 0.52 +0.1 825,270 + MMJJ_RS04420 0.52 +0.6 825,281 + MMJJ_RS04420 0.56 +0.1 825,287 - MMJJ_RS04420 0.58 +1.0 825,300 - MMJJ_RS04420 0.62 -0.3 825,308 - MMJJ_RS04420 0.65 +0.1 825,317 - MMJJ_RS04420 0.68 -0.3 825,342 + MMJJ_RS04420 0.77 +0.6 825,372 + MMJJ_RS04420 0.88 -0.5 825,373 - MMJJ_RS04420 0.88 +0.4 825,373 - MMJJ_RS04420 0.88 +0.2 825,374 + MMJJ_RS04420 0.88 +0.8 825,374 + MMJJ_RS04420 0.88 -0.4 825,374 + MMJJ_RS04420 0.88 -0.1 825,374 + MMJJ_RS04420 0.88 -0.4 825,398 - -0.8 825,398 - -0.4 825,400 - +1.0 825,400 - -2.1 825,400 - +0.0 825,400 - +0.9 825,400 - -1.2 825,400 - +0.6 825,401 + -0.2 825,401 + -1.4 825,481 + MMJJ_RS04425 0.36 -1.4 825,648 - +0.3 825,648 - +0.0 825,650 - -0.9 825,650 - -0.6 825,652 - -1.7 825,652 - -0.1 825,653 + +0.4 825,658 - -1.3 825,662 - -1.3 825,669 + -3.4 825,672 - -1.8 825,672 - -0.9 825,713 - -1.3 825,714 + -0.5 825,817 - MMJJ_RS04430 0.11 +0.8 825,817 - MMJJ_RS04430 0.11 +0.2 825,818 + MMJJ_RS04430 0.11 +0.7 825,822 - MMJJ_RS04430 0.12 +0.1 825,822 - MMJJ_RS04430 0.12 +0.8 825,823 + MMJJ_RS04430 0.12 -0.2 825,823 + MMJJ_RS04430 0.12 +0.7 825,827 - MMJJ_RS04430 0.13 +1.1 825,828 + MMJJ_RS04430 0.13 +0.2 825,828 + MMJJ_RS04430 0.13 +0.7 825,828 + MMJJ_RS04430 0.13 -0.0 825,828 + MMJJ_RS04430 0.13 +0.3 825,828 + MMJJ_RS04430 0.13 +0.8 825,828 + MMJJ_RS04430 0.13 -0.1 825,829 - MMJJ_RS04430 0.13 -0.4 825,830 + MMJJ_RS04430 0.13 +0.2 825,830 + MMJJ_RS04430 0.13 +0.5 825,892 - MMJJ_RS04430 0.22 -0.0 825,892 - MMJJ_RS04430 0.22 +0.8 825,897 - MMJJ_RS04430 0.23 +0.5 825,898 + MMJJ_RS04430 0.23 -0.2 825,901 - MMJJ_RS04430 0.23 -0.7 825,902 + MMJJ_RS04430 0.24 +0.8 825,902 + MMJJ_RS04430 0.24 +0.3 825,904 - MMJJ_RS04430 0.24 -0.3 825,905 + MMJJ_RS04430 0.24 +0.4 825,942 - MMJJ_RS04430 0.29 +0.5 825,955 + MMJJ_RS04430 0.31 +0.4 825,957 - MMJJ_RS04430 0.31 -0.0 825,958 + MMJJ_RS04430 0.32 -0.9 825,958 + MMJJ_RS04430 0.32 -1.5 825,962 - MMJJ_RS04430 0.32 -0.1 825,962 - MMJJ_RS04430 0.32 -0.7 825,962 - MMJJ_RS04430 0.32 -1.4 825,962 - MMJJ_RS04430 0.32 -0.2 825,995 + MMJJ_RS04430 0.37 -0.2 825,995 + MMJJ_RS04430 0.37 -0.4 826,083 - MMJJ_RS04430 0.50 +1.0 826,083 - MMJJ_RS04430 0.50 -0.1 826,084 + MMJJ_RS04430 0.50 +1.0 826,087 - MMJJ_RS04430 0.50 +0.5 826,097 + MMJJ_RS04430 0.52 -0.4 826,171 + MMJJ_RS04430 0.62 -0.8 826,172 - MMJJ_RS04430 0.62 -0.4 826,173 + MMJJ_RS04430 0.63 -0.1 826,180 - MMJJ_RS04430 0.64 -0.3 826,190 + MMJJ_RS04430 0.65 +0.5 826,222 + MMJJ_RS04430 0.70 +0.4 826,227 - MMJJ_RS04430 0.70 +1.1 826,227 - MMJJ_RS04430 0.70 +0.6 826,227 - MMJJ_RS04430 0.70 +0.7 826,228 + MMJJ_RS04430 0.71 +0.5 826,228 + MMJJ_RS04430 0.71 +0.6 826,245 + MMJJ_RS04430 0.73 +0.3
Or see this region's nucleotide sequence