Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04190 and MMJJ_RS04195 are separated by 113 nucleotides MMJJ_RS04195 and MMJJ_RS04200 are separated by 11 nucleotides MMJJ_RS04200 and MMJJ_RS04205 are separated by 11 nucleotides
MMJJ_RS04190: MMJJ_RS04190 - hypothetical protein, at 780,183 to 780,593
_RS04190
MMJJ_RS04195: MMJJ_RS04195 - XTP/dITP diphosphatase, at 780,707 to 781,261
_RS04195
MMJJ_RS04200: MMJJ_RS04200 - triphosphoribosyl-dephospho-CoA synthase, at 781,273 to 782,154
_RS04200
MMJJ_RS04205: MMJJ_RS04205 - glycine--tRNA ligase, at 782,166 to 783,890
_RS04205
Position (kb)
781
782
783 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 780.312 kb on - strand, within MMJJ_RS04190 at 780.325 kb on - strand, within MMJJ_RS04190 at 780.329 kb on - strand, within MMJJ_RS04190 at 780.330 kb on + strand, within MMJJ_RS04190 at 780.336 kb on + strand, within MMJJ_RS04190 at 780.344 kb on - strand, within MMJJ_RS04190 at 780.356 kb on - strand, within MMJJ_RS04190 at 780.356 kb on - strand, within MMJJ_RS04190 at 780.360 kb on - strand, within MMJJ_RS04190 at 780.360 kb on - strand, within MMJJ_RS04190 at 780.360 kb on - strand, within MMJJ_RS04190 at 780.360 kb on - strand, within MMJJ_RS04190 at 780.360 kb on - strand, within MMJJ_RS04190 at 780.360 kb on - strand, within MMJJ_RS04190 at 780.360 kb on - strand, within MMJJ_RS04190 at 780.360 kb on - strand, within MMJJ_RS04190 at 780.361 kb on + strand, within MMJJ_RS04190 at 780.361 kb on + strand, within MMJJ_RS04190 at 780.361 kb on + strand, within MMJJ_RS04190 at 780.361 kb on + strand, within MMJJ_RS04190 at 780.361 kb on + strand, within MMJJ_RS04190 at 780.361 kb on + strand, within MMJJ_RS04190 at 780.383 kb on + strand, within MMJJ_RS04190 at 780.391 kb on + strand, within MMJJ_RS04190 at 780.391 kb on + strand, within MMJJ_RS04190 at 780.553 kb on + strand at 780.567 kb on - strand at 780.594 kb on - strand at 780.595 kb on + strand at 780.613 kb on - strand at 780.614 kb on + strand at 780.614 kb on + strand at 780.614 kb on + strand at 780.639 kb on - strand at 780.641 kb on - strand at 780.642 kb on + strand at 780.642 kb on + strand at 780.645 kb on - strand at 780.645 kb on - strand at 780.645 kb on - strand at 780.645 kb on - strand at 780.646 kb on + strand at 780.669 kb on - strand at 780.673 kb on + strand at 780.746 kb on + strand at 780.760 kb on - strand at 780.763 kb on + strand, within MMJJ_RS04195 at 780.856 kb on + strand, within MMJJ_RS04195 at 780.888 kb on - strand, within MMJJ_RS04195 at 780.888 kb on - strand, within MMJJ_RS04195 at 780.888 kb on - strand, within MMJJ_RS04195 at 780.888 kb on - strand, within MMJJ_RS04195 at 780.888 kb on - strand, within MMJJ_RS04195 at 780.889 kb on + strand, within MMJJ_RS04195 at 780.889 kb on + strand, within MMJJ_RS04195 at 780.941 kb on - strand, within MMJJ_RS04195 at 780.941 kb on - strand, within MMJJ_RS04195 at 780.941 kb on - strand, within MMJJ_RS04195 at 780.942 kb on + strand, within MMJJ_RS04195 at 780.965 kb on + strand, within MMJJ_RS04195 at 781.043 kb on - strand, within MMJJ_RS04195 at 781.043 kb on - strand, within MMJJ_RS04195 at 781.044 kb on + strand, within MMJJ_RS04195 at 781.044 kb on + strand, within MMJJ_RS04195 at 781.066 kb on + strand, within MMJJ_RS04195 at 781.090 kb on - strand, within MMJJ_RS04195 at 781.162 kb on - strand, within MMJJ_RS04195 at 781.246 kb on + strand at 781.377 kb on + strand, within MMJJ_RS04200 at 781.382 kb on + strand, within MMJJ_RS04200 at 781.422 kb on + strand, within MMJJ_RS04200 at 781.449 kb on - strand, within MMJJ_RS04200 at 781.478 kb on - strand, within MMJJ_RS04200 at 781.479 kb on + strand, within MMJJ_RS04200 at 781.523 kb on - strand, within MMJJ_RS04200 at 781.528 kb on + strand, within MMJJ_RS04200 at 781.649 kb on + strand, within MMJJ_RS04200 at 781.713 kb on - strand, within MMJJ_RS04200 at 781.714 kb on + strand, within MMJJ_RS04200 at 781.891 kb on + strand, within MMJJ_RS04200 at 781.910 kb on - strand, within MMJJ_RS04200 at 781.910 kb on - strand, within MMJJ_RS04200 at 781.934 kb on - strand, within MMJJ_RS04200 at 781.953 kb on - strand, within MMJJ_RS04200 at 781.986 kb on - strand, within MMJJ_RS04200 at 782.319 kb on - strand at 782.998 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 780,312 - MMJJ_RS04190 0.31 -0.4 780,325 - MMJJ_RS04190 0.35 +0.5 780,329 - MMJJ_RS04190 0.36 +0.7 780,330 + MMJJ_RS04190 0.36 +0.2 780,336 + MMJJ_RS04190 0.37 +0.1 780,344 - MMJJ_RS04190 0.39 +1.2 780,356 - MMJJ_RS04190 0.42 +0.4 780,356 - MMJJ_RS04190 0.42 +0.8 780,360 - MMJJ_RS04190 0.43 -0.3 780,360 - MMJJ_RS04190 0.43 +0.1 780,360 - MMJJ_RS04190 0.43 +0.3 780,360 - MMJJ_RS04190 0.43 +1.9 780,360 - MMJJ_RS04190 0.43 -0.5 780,360 - MMJJ_RS04190 0.43 -0.5 780,360 - MMJJ_RS04190 0.43 -0.3 780,360 - MMJJ_RS04190 0.43 -0.3 780,361 + MMJJ_RS04190 0.43 +0.4 780,361 + MMJJ_RS04190 0.43 +0.7 780,361 + MMJJ_RS04190 0.43 +0.3 780,361 + MMJJ_RS04190 0.43 -0.6 780,361 + MMJJ_RS04190 0.43 -0.0 780,361 + MMJJ_RS04190 0.43 -0.4 780,383 + MMJJ_RS04190 0.49 +1.4 780,391 + MMJJ_RS04190 0.51 -1.0 780,391 + MMJJ_RS04190 0.51 +0.4 780,553 + +0.2 780,567 - -0.2 780,594 - +0.3 780,595 + -0.0 780,613 - -0.7 780,614 + -0.4 780,614 + -0.2 780,614 + -0.6 780,639 - +0.6 780,641 - +0.1 780,642 + -0.6 780,642 + +1.0 780,645 - +0.6 780,645 - +0.0 780,645 - -0.1 780,645 - +0.8 780,646 + -1.1 780,669 - -0.5 780,673 + +0.8 780,746 + -0.4 780,760 - -1.0 780,763 + MMJJ_RS04195 0.10 +0.7 780,856 + MMJJ_RS04195 0.27 +0.2 780,888 - MMJJ_RS04195 0.33 -0.4 780,888 - MMJJ_RS04195 0.33 -0.5 780,888 - MMJJ_RS04195 0.33 -1.9 780,888 - MMJJ_RS04195 0.33 +0.2 780,888 - MMJJ_RS04195 0.33 -1.0 780,889 + MMJJ_RS04195 0.33 -0.1 780,889 + MMJJ_RS04195 0.33 -0.1 780,941 - MMJJ_RS04195 0.42 -0.1 780,941 - MMJJ_RS04195 0.42 -0.5 780,941 - MMJJ_RS04195 0.42 -4.4 780,942 + MMJJ_RS04195 0.42 -0.1 780,965 + MMJJ_RS04195 0.46 -0.6 781,043 - MMJJ_RS04195 0.61 +0.4 781,043 - MMJJ_RS04195 0.61 +0.0 781,044 + MMJJ_RS04195 0.61 -0.9 781,044 + MMJJ_RS04195 0.61 -0.2 781,066 + MMJJ_RS04195 0.65 +0.3 781,090 - MMJJ_RS04195 0.69 -0.8 781,162 - MMJJ_RS04195 0.82 +0.7 781,246 + +0.3 781,377 + MMJJ_RS04200 0.12 -0.7 781,382 + MMJJ_RS04200 0.12 -1.7 781,422 + MMJJ_RS04200 0.17 -2.9 781,449 - MMJJ_RS04200 0.20 -3.2 781,478 - MMJJ_RS04200 0.23 -4.3 781,479 + MMJJ_RS04200 0.23 -5.2 781,523 - MMJJ_RS04200 0.28 -4.4 781,528 + MMJJ_RS04200 0.29 -3.1 781,649 + MMJJ_RS04200 0.43 -3.8 781,713 - MMJJ_RS04200 0.50 -2.7 781,714 + MMJJ_RS04200 0.50 -0.4 781,891 + MMJJ_RS04200 0.70 -2.6 781,910 - MMJJ_RS04200 0.72 -4.4 781,910 - MMJJ_RS04200 0.72 -4.4 781,934 - MMJJ_RS04200 0.75 -3.9 781,953 - MMJJ_RS04200 0.77 -2.3 781,986 - MMJJ_RS04200 0.81 -3.2 782,319 - -0.4 782,998 + -1.5
Or see this region's nucleotide sequence