Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03890 and MMJJ_RS03895 are separated by 250 nucleotides MMJJ_RS03895 and MMJJ_RS03900 are separated by 211 nucleotides MMJJ_RS03900 and MMJJ_RS03905 are separated by 18 nucleotides
MMJJ_RS03890: MMJJ_RS03890 - right-handed parallel beta-helix repeat-containing protein, at 724,579 to 727,356
_RS03890
MMJJ_RS03895: MMJJ_RS03895 - hydrogenase expression/formation protein HypE, at 727,607 to 728,611
_RS03895
MMJJ_RS03900: MMJJ_RS03900 - phosphosulfolactate synthase, at 728,823 to 729,578
_RS03900
MMJJ_RS03905: MMJJ_RS03905 - ATP-binding cassette domain-containing protein, at 729,597 to 730,388
_RS03905
Position (kb) 727
728
729 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 726.619 kb on + strand, within MMJJ_RS03890 at 726.619 kb on + strand, within MMJJ_RS03890 at 726.619 kb on + strand, within MMJJ_RS03890 at 726.641 kb on + strand, within MMJJ_RS03890 at 726.676 kb on + strand, within MMJJ_RS03890 at 726.679 kb on - strand, within MMJJ_RS03890 at 726.680 kb on + strand, within MMJJ_RS03890 at 726.720 kb on - strand, within MMJJ_RS03890 at 726.721 kb on + strand, within MMJJ_RS03890 at 726.753 kb on - strand, within MMJJ_RS03890 at 726.754 kb on + strand, within MMJJ_RS03890 at 726.754 kb on + strand, within MMJJ_RS03890 at 726.772 kb on - strand, within MMJJ_RS03890 at 726.791 kb on - strand, within MMJJ_RS03890 at 726.802 kb on + strand, within MMJJ_RS03890 at 726.802 kb on + strand, within MMJJ_RS03890 at 726.805 kb on - strand, within MMJJ_RS03890 at 726.823 kb on + strand, within MMJJ_RS03890 at 726.823 kb on + strand, within MMJJ_RS03890 at 726.844 kb on - strand, within MMJJ_RS03890 at 726.844 kb on - strand, within MMJJ_RS03890 at 726.845 kb on + strand, within MMJJ_RS03890 at 726.861 kb on - strand, within MMJJ_RS03890 at 726.906 kb on - strand, within MMJJ_RS03890 at 726.907 kb on + strand, within MMJJ_RS03890 at 726.932 kb on - strand, within MMJJ_RS03890 at 726.944 kb on - strand, within MMJJ_RS03890 at 726.945 kb on + strand, within MMJJ_RS03890 at 726.945 kb on + strand, within MMJJ_RS03890 at 726.945 kb on + strand, within MMJJ_RS03890 at 726.945 kb on + strand, within MMJJ_RS03890 at 726.948 kb on - strand, within MMJJ_RS03890 at 727.003 kb on - strand, within MMJJ_RS03890 at 727.003 kb on - strand, within MMJJ_RS03890 at 727.003 kb on - strand, within MMJJ_RS03890 at 727.004 kb on + strand, within MMJJ_RS03890 at 727.045 kb on - strand, within MMJJ_RS03890 at 727.046 kb on + strand, within MMJJ_RS03890 at 727.046 kb on + strand, within MMJJ_RS03890 at 727.046 kb on + strand, within MMJJ_RS03890 at 727.067 kb on + strand, within MMJJ_RS03890 at 727.083 kb on + strand at 727.098 kb on - strand at 727.098 kb on - strand at 727.099 kb on + strand at 727.099 kb on + strand at 727.109 kb on + strand at 727.118 kb on + strand at 727.125 kb on - strand at 727.132 kb on + strand at 727.167 kb on - strand at 727.168 kb on + strand at 727.171 kb on - strand at 727.172 kb on + strand at 727.224 kb on - strand at 727.260 kb on + strand at 727.269 kb on - strand at 727.270 kb on + strand at 727.273 kb on - strand at 727.277 kb on - strand at 727.278 kb on + strand at 727.278 kb on + strand at 727.278 kb on + strand at 727.278 kb on + strand at 727.278 kb on + strand at 727.278 kb on + strand at 727.279 kb on - strand at 727.279 kb on - strand at 727.279 kb on - strand at 727.279 kb on - strand at 727.280 kb on + strand at 727.285 kb on + strand at 727.285 kb on + strand at 727.287 kb on - strand at 727.287 kb on - strand at 727.300 kb on - strand at 727.321 kb on - strand at 727.324 kb on - strand at 727.324 kb on - strand at 727.339 kb on - strand at 727.339 kb on - strand at 727.340 kb on + strand at 727.340 kb on + strand at 727.358 kb on + strand at 727.380 kb on + strand at 727.380 kb on + strand at 727.380 kb on + strand at 727.404 kb on - strand at 727.405 kb on + strand at 727.405 kb on + strand at 727.411 kb on - strand at 727.418 kb on + strand at 727.420 kb on + strand at 727.439 kb on + strand at 727.444 kb on + strand at 727.899 kb on + strand, within MMJJ_RS03895 at 727.925 kb on - strand, within MMJJ_RS03895 at 728.077 kb on - strand, within MMJJ_RS03895 at 728.231 kb on - strand, within MMJJ_RS03895 at 728.231 kb on - strand, within MMJJ_RS03895 at 728.427 kb on + strand, within MMJJ_RS03895 at 728.471 kb on + strand, within MMJJ_RS03895 at 728.535 kb on + strand at 728.629 kb on - strand at 728.640 kb on - strand at 728.640 kb on - strand at 728.640 kb on - strand at 728.644 kb on - strand at 728.644 kb on - strand at 728.661 kb on + strand at 728.681 kb on + strand at 728.691 kb on + strand at 728.691 kb on + strand at 728.703 kb on + strand at 728.732 kb on - strand at 728.732 kb on - strand at 728.732 kb on - strand at 728.735 kb on + strand at 728.736 kb on - strand at 728.743 kb on - strand at 728.750 kb on - strand at 728.750 kb on - strand at 728.750 kb on - strand at 728.750 kb on - strand at 728.750 kb on - strand at 728.751 kb on + strand at 728.751 kb on + strand at 728.752 kb on - strand at 728.753 kb on + strand at 728.755 kb on - strand at 728.756 kb on + strand at 728.756 kb on + strand at 728.760 kb on - strand at 728.795 kb on + strand at 728.868 kb on + strand at 728.875 kb on - strand at 728.876 kb on + strand at 728.918 kb on + strand, within MMJJ_RS03900 at 728.923 kb on - strand, within MMJJ_RS03900 at 728.935 kb on + strand, within MMJJ_RS03900 at 729.061 kb on - strand, within MMJJ_RS03900 at 729.061 kb on - strand, within MMJJ_RS03900 at 729.061 kb on - strand, within MMJJ_RS03900 at 729.061 kb on - strand, within MMJJ_RS03900 at 729.062 kb on + strand, within MMJJ_RS03900 at 729.062 kb on + strand, within MMJJ_RS03900 at 729.090 kb on - strand, within MMJJ_RS03900 at 729.091 kb on + strand, within MMJJ_RS03900 at 729.095 kb on + strand, within MMJJ_RS03900 at 729.095 kb on + strand, within MMJJ_RS03900 at 729.095 kb on + strand, within MMJJ_RS03900 at 729.104 kb on - strand, within MMJJ_RS03900 at 729.104 kb on - strand, within MMJJ_RS03900 at 729.104 kb on - strand, within MMJJ_RS03900 at 729.105 kb on + strand, within MMJJ_RS03900 at 729.113 kb on + strand, within MMJJ_RS03900 at 729.275 kb on + strand, within MMJJ_RS03900 at 729.397 kb on - strand, within MMJJ_RS03900 at 729.412 kb on - strand, within MMJJ_RS03900 at 729.427 kb on - strand, within MMJJ_RS03900 at 729.468 kb on + strand, within MMJJ_RS03900 at 729.468 kb on + strand, within MMJJ_RS03900 at 729.469 kb on - strand, within MMJJ_RS03900 at 729.472 kb on - strand, within MMJJ_RS03900 at 729.473 kb on + strand, within MMJJ_RS03900 at 729.503 kb on - strand at 729.504 kb on + strand at 729.504 kb on + strand at 729.580 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 726,619 + MMJJ_RS03890 0.73 +0.5 726,619 + MMJJ_RS03890 0.73 -0.0 726,619 + MMJJ_RS03890 0.73 -1.3 726,641 + MMJJ_RS03890 0.74 +1.0 726,676 + MMJJ_RS03890 0.75 -1.1 726,679 - MMJJ_RS03890 0.76 -0.7 726,680 + MMJJ_RS03890 0.76 -0.7 726,720 - MMJJ_RS03890 0.77 +0.6 726,721 + MMJJ_RS03890 0.77 +0.0 726,753 - MMJJ_RS03890 0.78 -0.3 726,754 + MMJJ_RS03890 0.78 -0.5 726,754 + MMJJ_RS03890 0.78 -1.2 726,772 - MMJJ_RS03890 0.79 +1.0 726,791 - MMJJ_RS03890 0.80 +0.0 726,802 + MMJJ_RS03890 0.80 -0.4 726,802 + MMJJ_RS03890 0.80 +0.0 726,805 - MMJJ_RS03890 0.80 -0.2 726,823 + MMJJ_RS03890 0.81 +0.2 726,823 + MMJJ_RS03890 0.81 +0.3 726,844 - MMJJ_RS03890 0.82 +0.3 726,844 - MMJJ_RS03890 0.82 -0.3 726,845 + MMJJ_RS03890 0.82 +0.0 726,861 - MMJJ_RS03890 0.82 -0.2 726,906 - MMJJ_RS03890 0.84 +0.5 726,907 + MMJJ_RS03890 0.84 +0.6 726,932 - MMJJ_RS03890 0.85 -1.4 726,944 - MMJJ_RS03890 0.85 +1.2 726,945 + MMJJ_RS03890 0.85 +0.0 726,945 + MMJJ_RS03890 0.85 +0.8 726,945 + MMJJ_RS03890 0.85 +0.9 726,945 + MMJJ_RS03890 0.85 +0.3 726,948 - MMJJ_RS03890 0.85 +0.2 727,003 - MMJJ_RS03890 0.87 +0.2 727,003 - MMJJ_RS03890 0.87 +0.3 727,003 - MMJJ_RS03890 0.87 +0.6 727,004 + MMJJ_RS03890 0.87 -0.6 727,045 - MMJJ_RS03890 0.89 -0.8 727,046 + MMJJ_RS03890 0.89 +0.7 727,046 + MMJJ_RS03890 0.89 +0.5 727,046 + MMJJ_RS03890 0.89 +0.6 727,067 + MMJJ_RS03890 0.90 -0.5 727,083 + +0.7 727,098 - +0.2 727,098 - +0.3 727,099 + +0.1 727,099 + +0.8 727,109 + +0.9 727,118 + +0.5 727,125 - +0.6 727,132 + +0.1 727,167 - -0.1 727,168 + -0.2 727,171 - +0.2 727,172 + -0.3 727,224 - -0.3 727,260 + +0.8 727,269 - +1.1 727,270 + -0.1 727,273 - +0.2 727,277 - -1.5 727,278 + +0.4 727,278 + -1.9 727,278 + +0.3 727,278 + -1.2 727,278 + +0.2 727,278 + +0.7 727,279 - +0.9 727,279 - +0.3 727,279 - +1.4 727,279 - +0.1 727,280 + -0.4 727,285 + -0.7 727,285 + -0.7 727,287 - +0.9 727,287 - -0.2 727,300 - +0.1 727,321 - +0.1 727,324 - -0.9 727,324 - -0.5 727,339 - +0.6 727,339 - +0.7 727,340 + +0.6 727,340 + +0.1 727,358 + -0.4 727,380 + -0.4 727,380 + +0.0 727,380 + -0.2 727,404 - +0.6 727,405 + +0.2 727,405 + +0.6 727,411 - +0.8 727,418 + +0.0 727,420 + -0.2 727,439 + -1.9 727,444 + -1.2 727,899 + MMJJ_RS03895 0.29 -3.1 727,925 - MMJJ_RS03895 0.32 -3.4 728,077 - MMJJ_RS03895 0.47 -3.1 728,231 - MMJJ_RS03895 0.62 -2.0 728,231 - MMJJ_RS03895 0.62 -3.6 728,427 + MMJJ_RS03895 0.82 -0.7 728,471 + MMJJ_RS03895 0.86 -2.3 728,535 + -3.9 728,629 - -0.4 728,640 - -0.3 728,640 - -0.9 728,640 - +0.3 728,644 - -0.2 728,644 - +1.3 728,661 + +0.7 728,681 + -1.1 728,691 + -1.2 728,691 + -0.8 728,703 + -0.0 728,732 - +0.8 728,732 - -0.7 728,732 - -1.0 728,735 + -0.0 728,736 - -0.0 728,743 - -0.3 728,750 - +0.0 728,750 - +0.1 728,750 - +0.5 728,750 - +0.2 728,750 - +0.2 728,751 + +0.2 728,751 + -0.5 728,752 - -0.4 728,753 + -0.5 728,755 - +0.6 728,756 + -0.7 728,756 + +0.6 728,760 - -0.4 728,795 + +1.1 728,868 + +0.6 728,875 - +0.5 728,876 + +0.2 728,918 + MMJJ_RS03900 0.13 +0.4 728,923 - MMJJ_RS03900 0.13 -0.1 728,935 + MMJJ_RS03900 0.15 +0.3 729,061 - MMJJ_RS03900 0.31 +0.7 729,061 - MMJJ_RS03900 0.31 -0.4 729,061 - MMJJ_RS03900 0.31 +0.6 729,061 - MMJJ_RS03900 0.31 +2.3 729,062 + MMJJ_RS03900 0.32 -0.4 729,062 + MMJJ_RS03900 0.32 -0.2 729,090 - MMJJ_RS03900 0.35 +0.4 729,091 + MMJJ_RS03900 0.35 +0.1 729,095 + MMJJ_RS03900 0.36 +0.1 729,095 + MMJJ_RS03900 0.36 -1.2 729,095 + MMJJ_RS03900 0.36 +0.6 729,104 - MMJJ_RS03900 0.37 -0.3 729,104 - MMJJ_RS03900 0.37 -0.7 729,104 - MMJJ_RS03900 0.37 +0.6 729,105 + MMJJ_RS03900 0.37 +0.0 729,113 + MMJJ_RS03900 0.38 -0.9 729,275 + MMJJ_RS03900 0.60 -1.8 729,397 - MMJJ_RS03900 0.76 -0.1 729,412 - MMJJ_RS03900 0.78 -0.5 729,427 - MMJJ_RS03900 0.80 -0.8 729,468 + MMJJ_RS03900 0.85 -0.5 729,468 + MMJJ_RS03900 0.85 +0.6 729,469 - MMJJ_RS03900 0.85 +0.1 729,472 - MMJJ_RS03900 0.86 -0.3 729,473 + MMJJ_RS03900 0.86 +0.0 729,503 - -0.4 729,504 + +0.2 729,504 + -0.7 729,580 - +0.6
Or see this region's nucleotide sequence