Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03825 and MMJJ_RS03830 are separated by 37 nucleotides MMJJ_RS03830 and MMJJ_RS03835 are separated by 5 nucleotides MMJJ_RS03835 and MMJJ_RS03840 are separated by 9 nucleotides
MMJJ_RS03825: MMJJ_RS03825 - DUF1624 domain-containing protein, at 711,892 to 712,632
_RS03825
MMJJ_RS03830: MMJJ_RS03830 - CBS domain-containing protein, at 712,670 to 713,464
_RS03830
MMJJ_RS03835: MMJJ_RS03835 - DUF504 domain-containing protein, at 713,470 to 713,700
_RS03835
MMJJ_RS03840: MMJJ_RS03840 - DHH family phosphoesterase, at 713,710 to 715,170
_RS03840
Position (kb)
713
714 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 712.518 kb on + strand, within MMJJ_RS03825 at 712.518 kb on + strand, within MMJJ_RS03825 at 712.544 kb on + strand, within MMJJ_RS03825 at 712.553 kb on + strand, within MMJJ_RS03825 at 712.567 kb on + strand at 712.567 kb on + strand at 712.576 kb on - strand at 712.576 kb on - strand at 712.577 kb on + strand at 712.577 kb on + strand at 712.579 kb on - strand at 712.650 kb on + strand at 712.658 kb on + strand at 712.684 kb on - strand at 712.699 kb on - strand at 712.700 kb on + strand at 712.700 kb on + strand at 712.700 kb on + strand at 712.700 kb on + strand at 712.700 kb on + strand at 712.701 kb on - strand at 712.741 kb on - strand at 712.745 kb on + strand at 712.811 kb on - strand, within MMJJ_RS03830 at 712.893 kb on - strand, within MMJJ_RS03830 at 712.919 kb on + strand, within MMJJ_RS03830 at 712.949 kb on - strand, within MMJJ_RS03830 at 712.950 kb on + strand, within MMJJ_RS03830 at 712.957 kb on + strand, within MMJJ_RS03830 at 713.006 kb on - strand, within MMJJ_RS03830 at 713.006 kb on - strand, within MMJJ_RS03830 at 713.007 kb on + strand, within MMJJ_RS03830 at 713.024 kb on + strand, within MMJJ_RS03830 at 713.038 kb on - strand, within MMJJ_RS03830 at 713.039 kb on + strand, within MMJJ_RS03830 at 713.039 kb on + strand, within MMJJ_RS03830 at 713.039 kb on + strand, within MMJJ_RS03830 at 713.043 kb on + strand, within MMJJ_RS03830 at 713.065 kb on + strand, within MMJJ_RS03830 at 713.124 kb on + strand, within MMJJ_RS03830 at 713.124 kb on + strand, within MMJJ_RS03830 at 713.124 kb on + strand, within MMJJ_RS03830 at 713.124 kb on + strand, within MMJJ_RS03830 at 713.124 kb on + strand, within MMJJ_RS03830 at 713.197 kb on - strand, within MMJJ_RS03830 at 713.198 kb on + strand, within MMJJ_RS03830 at 713.222 kb on - strand, within MMJJ_RS03830 at 713.242 kb on - strand, within MMJJ_RS03830 at 713.280 kb on + strand, within MMJJ_RS03830 at 713.299 kb on - strand, within MMJJ_RS03830 at 713.306 kb on + strand, within MMJJ_RS03830 at 713.391 kb on + strand at 713.446 kb on + strand at 713.459 kb on - strand at 713.460 kb on + strand at 713.506 kb on + strand, within MMJJ_RS03835 at 713.580 kb on - strand, within MMJJ_RS03835 at 713.630 kb on - strand, within MMJJ_RS03835 at 713.631 kb on + strand, within MMJJ_RS03835 at 713.640 kb on - strand, within MMJJ_RS03835 at 713.643 kb on + strand, within MMJJ_RS03835 at 713.643 kb on + strand, within MMJJ_RS03835 at 713.656 kb on + strand, within MMJJ_RS03835 at 713.686 kb on + strand at 713.686 kb on + strand at 713.701 kb on - strand at 713.701 kb on - strand at 713.701 kb on - strand at 713.702 kb on + strand at 713.711 kb on - strand at 713.966 kb on - strand, within MMJJ_RS03840 at 714.027 kb on - strand, within MMJJ_RS03840 at 714.248 kb on - strand, within MMJJ_RS03840 at 714.517 kb on - strand, within MMJJ_RS03840 at 714.517 kb on - strand, within MMJJ_RS03840 at 714.545 kb on - strand, within MMJJ_RS03840 at 714.546 kb on + strand, within MMJJ_RS03840 at 714.658 kb on - strand, within MMJJ_RS03840 at 714.662 kb on + strand, within MMJJ_RS03840
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 712,518 + MMJJ_RS03825 0.84 -0.5 712,518 + MMJJ_RS03825 0.84 -2.3 712,544 + MMJJ_RS03825 0.88 +1.2 712,553 + MMJJ_RS03825 0.89 -0.3 712,567 + -0.1 712,567 + +0.9 712,576 - -0.6 712,576 - +0.9 712,577 + -0.5 712,577 + +0.2 712,579 - +0.2 712,650 + -0.1 712,658 + -0.0 712,684 - -0.9 712,699 - -0.3 712,700 + +0.0 712,700 + -0.3 712,700 + -0.1 712,700 + -0.8 712,700 + -0.8 712,701 - -0.5 712,741 - -0.1 712,745 + -1.1 712,811 - MMJJ_RS03830 0.18 -1.8 712,893 - MMJJ_RS03830 0.28 -3.9 712,919 + MMJJ_RS03830 0.31 -1.1 712,949 - MMJJ_RS03830 0.35 -2.8 712,950 + MMJJ_RS03830 0.35 -2.2 712,957 + MMJJ_RS03830 0.36 -2.2 713,006 - MMJJ_RS03830 0.42 -1.4 713,006 - MMJJ_RS03830 0.42 -2.9 713,007 + MMJJ_RS03830 0.42 -2.1 713,024 + MMJJ_RS03830 0.45 -1.6 713,038 - MMJJ_RS03830 0.46 -1.8 713,039 + MMJJ_RS03830 0.46 -1.5 713,039 + MMJJ_RS03830 0.46 -2.5 713,039 + MMJJ_RS03830 0.46 -3.0 713,043 + MMJJ_RS03830 0.47 -2.9 713,065 + MMJJ_RS03830 0.50 -1.8 713,124 + MMJJ_RS03830 0.57 -2.5 713,124 + MMJJ_RS03830 0.57 -2.6 713,124 + MMJJ_RS03830 0.57 -1.7 713,124 + MMJJ_RS03830 0.57 -2.3 713,124 + MMJJ_RS03830 0.57 -0.7 713,197 - MMJJ_RS03830 0.66 -5.7 713,198 + MMJJ_RS03830 0.66 -1.0 713,222 - MMJJ_RS03830 0.69 -1.8 713,242 - MMJJ_RS03830 0.72 -0.5 713,280 + MMJJ_RS03830 0.77 -1.0 713,299 - MMJJ_RS03830 0.79 -2.2 713,306 + MMJJ_RS03830 0.80 -0.8 713,391 + -3.0 713,446 + -1.0 713,459 - -2.0 713,460 + -2.0 713,506 + MMJJ_RS03835 0.16 -0.1 713,580 - MMJJ_RS03835 0.48 +0.1 713,630 - MMJJ_RS03835 0.69 +0.2 713,631 + MMJJ_RS03835 0.70 +0.5 713,640 - MMJJ_RS03835 0.74 -0.6 713,643 + MMJJ_RS03835 0.75 +0.6 713,643 + MMJJ_RS03835 0.75 +0.3 713,656 + MMJJ_RS03835 0.81 +1.2 713,686 + +1.6 713,686 + +1.3 713,701 - +1.1 713,701 - +0.9 713,701 - +0.0 713,702 + -0.6 713,711 - +0.6 713,966 - MMJJ_RS03840 0.18 -0.7 714,027 - MMJJ_RS03840 0.22 -3.1 714,248 - MMJJ_RS03840 0.37 -1.7 714,517 - MMJJ_RS03840 0.55 -2.9 714,517 - MMJJ_RS03840 0.55 -2.5 714,545 - MMJJ_RS03840 0.57 -3.3 714,546 + MMJJ_RS03840 0.57 -2.9 714,658 - MMJJ_RS03840 0.65 -2.1 714,662 + MMJJ_RS03840 0.65 -1.6
Or see this region's nucleotide sequence