Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03620 and MMJJ_RS03625 are separated by 13 nucleotides MMJJ_RS03625 and MMJJ_RS03630 are separated by 109 nucleotides MMJJ_RS03630 and MMJJ_RS03635 are separated by 135 nucleotides
MMJJ_RS03620: MMJJ_RS03620 - DUF2097 domain-containing protein, at 674,364 to 674,654
_RS03620
MMJJ_RS03625: MMJJ_RS03625 - DUF63 family protein, at 674,668 to 675,474
_RS03625
MMJJ_RS03630: MMJJ_RS03630 - AMP phosphorylase, at 675,584 to 677,101
_RS03630
MMJJ_RS03635: MMJJ_RS03635 - methionine--tRNA ligase, at 677,237 to 679,228
_RS03635
Position (kb) 675
676
677
678 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 674.590 kb on - strand, within MMJJ_RS03620 at 674.590 kb on - strand, within MMJJ_RS03620 at 674.591 kb on + strand, within MMJJ_RS03620 at 674.638 kb on - strand at 674.639 kb on + strand at 674.655 kb on + strand at 674.669 kb on - strand at 674.688 kb on - strand at 674.689 kb on + strand at 674.689 kb on + strand at 674.689 kb on + strand at 674.692 kb on - strand at 674.735 kb on + strand at 674.741 kb on + strand at 674.743 kb on - strand at 674.744 kb on + strand at 674.745 kb on - strand at 674.745 kb on - strand at 674.746 kb on + strand at 674.746 kb on + strand at 674.782 kb on - strand, within MMJJ_RS03625 at 674.782 kb on - strand, within MMJJ_RS03625 at 674.782 kb on - strand, within MMJJ_RS03625 at 674.783 kb on + strand, within MMJJ_RS03625 at 674.783 kb on + strand, within MMJJ_RS03625 at 674.783 kb on + strand, within MMJJ_RS03625 at 674.821 kb on - strand, within MMJJ_RS03625 at 674.822 kb on + strand, within MMJJ_RS03625 at 674.825 kb on + strand, within MMJJ_RS03625 at 674.832 kb on + strand, within MMJJ_RS03625 at 674.888 kb on - strand, within MMJJ_RS03625 at 674.888 kb on - strand, within MMJJ_RS03625 at 674.890 kb on - strand, within MMJJ_RS03625 at 674.891 kb on + strand, within MMJJ_RS03625 at 674.923 kb on - strand, within MMJJ_RS03625 at 674.923 kb on - strand, within MMJJ_RS03625 at 674.939 kb on + strand, within MMJJ_RS03625 at 674.945 kb on - strand, within MMJJ_RS03625 at 674.946 kb on + strand, within MMJJ_RS03625 at 674.954 kb on + strand, within MMJJ_RS03625 at 674.994 kb on + strand, within MMJJ_RS03625 at 675.005 kb on - strand, within MMJJ_RS03625 at 675.006 kb on + strand, within MMJJ_RS03625 at 675.006 kb on + strand, within MMJJ_RS03625 at 675.008 kb on - strand, within MMJJ_RS03625 at 675.009 kb on + strand, within MMJJ_RS03625 at 675.013 kb on - strand, within MMJJ_RS03625 at 675.013 kb on - strand, within MMJJ_RS03625 at 675.014 kb on + strand, within MMJJ_RS03625 at 675.035 kb on - strand, within MMJJ_RS03625 at 675.036 kb on + strand, within MMJJ_RS03625 at 675.086 kb on - strand, within MMJJ_RS03625 at 675.087 kb on + strand, within MMJJ_RS03625 at 675.100 kb on - strand, within MMJJ_RS03625 at 675.100 kb on - strand, within MMJJ_RS03625 at 675.113 kb on + strand, within MMJJ_RS03625 at 675.121 kb on - strand, within MMJJ_RS03625 at 675.125 kb on - strand, within MMJJ_RS03625 at 675.126 kb on + strand, within MMJJ_RS03625 at 675.126 kb on + strand, within MMJJ_RS03625 at 675.129 kb on + strand, within MMJJ_RS03625 at 675.129 kb on + strand, within MMJJ_RS03625 at 675.156 kb on - strand, within MMJJ_RS03625 at 675.157 kb on + strand, within MMJJ_RS03625 at 675.160 kb on - strand, within MMJJ_RS03625 at 675.177 kb on + strand, within MMJJ_RS03625 at 675.177 kb on + strand, within MMJJ_RS03625 at 675.197 kb on + strand, within MMJJ_RS03625 at 675.216 kb on + strand, within MMJJ_RS03625 at 675.278 kb on - strand, within MMJJ_RS03625 at 675.278 kb on - strand, within MMJJ_RS03625 at 675.288 kb on - strand, within MMJJ_RS03625 at 675.289 kb on + strand, within MMJJ_RS03625 at 675.289 kb on + strand, within MMJJ_RS03625 at 675.394 kb on - strand at 675.400 kb on + strand at 675.429 kb on - strand at 675.429 kb on - strand at 675.429 kb on - strand at 675.429 kb on - strand at 675.429 kb on - strand at 675.429 kb on - strand at 675.429 kb on - strand at 675.429 kb on - strand at 675.430 kb on + strand at 675.430 kb on + strand at 675.431 kb on - strand at 675.431 kb on - strand at 675.431 kb on - strand at 675.431 kb on - strand at 675.431 kb on - strand at 675.432 kb on + strand at 675.490 kb on + strand at 675.496 kb on + strand at 675.504 kb on - strand at 675.536 kb on + strand at 675.539 kb on - strand at 675.546 kb on + strand at 675.552 kb on + strand at 675.555 kb on - strand at 675.561 kb on - strand at 675.562 kb on + strand at 675.565 kb on - strand at 675.565 kb on - strand at 675.565 kb on - strand at 675.565 kb on - strand at 675.565 kb on - strand at 675.565 kb on - strand at 675.565 kb on - strand at 675.566 kb on + strand at 675.566 kb on + strand at 675.566 kb on + strand at 675.566 kb on + strand at 675.566 kb on + strand at 675.566 kb on + strand at 675.566 kb on + strand at 675.568 kb on + strand at 675.568 kb on + strand at 675.596 kb on - strand at 675.597 kb on + strand at 675.597 kb on + strand at 675.633 kb on - strand at 675.634 kb on + strand at 675.653 kb on - strand at 675.688 kb on + strand at 675.694 kb on - strand at 675.694 kb on - strand at 675.695 kb on + strand at 675.695 kb on + strand at 675.696 kb on - strand at 675.696 kb on - strand at 675.716 kb on + strand at 675.743 kb on - strand, within MMJJ_RS03630 at 675.808 kb on - strand, within MMJJ_RS03630 at 675.809 kb on + strand, within MMJJ_RS03630 at 675.813 kb on + strand, within MMJJ_RS03630 at 675.814 kb on - strand, within MMJJ_RS03630 at 675.833 kb on - strand, within MMJJ_RS03630 at 675.950 kb on - strand, within MMJJ_RS03630 at 676.140 kb on - strand, within MMJJ_RS03630 at 676.141 kb on + strand, within MMJJ_RS03630 at 676.141 kb on + strand, within MMJJ_RS03630 at 676.146 kb on + strand, within MMJJ_RS03630 at 676.255 kb on - strand, within MMJJ_RS03630 at 676.269 kb on - strand, within MMJJ_RS03630 at 676.269 kb on - strand, within MMJJ_RS03630 at 676.478 kb on - strand, within MMJJ_RS03630 at 676.479 kb on + strand, within MMJJ_RS03630 at 676.479 kb on + strand, within MMJJ_RS03630 at 676.554 kb on + strand, within MMJJ_RS03630 at 676.576 kb on + strand, within MMJJ_RS03630 at 676.576 kb on + strand, within MMJJ_RS03630 at 676.600 kb on - strand, within MMJJ_RS03630 at 676.680 kb on + strand, within MMJJ_RS03630 at 676.680 kb on + strand, within MMJJ_RS03630 at 676.703 kb on + strand, within MMJJ_RS03630 at 676.707 kb on - strand, within MMJJ_RS03630 at 676.707 kb on - strand, within MMJJ_RS03630 at 676.708 kb on + strand, within MMJJ_RS03630 at 676.708 kb on + strand, within MMJJ_RS03630 at 676.711 kb on - strand, within MMJJ_RS03630 at 676.711 kb on - strand, within MMJJ_RS03630 at 676.711 kb on - strand, within MMJJ_RS03630 at 676.712 kb on + strand, within MMJJ_RS03630 at 676.781 kb on - strand, within MMJJ_RS03630 at 676.781 kb on - strand, within MMJJ_RS03630 at 676.796 kb on - strand, within MMJJ_RS03630 at 676.825 kb on - strand, within MMJJ_RS03630 at 676.859 kb on - strand, within MMJJ_RS03630 at 676.860 kb on + strand, within MMJJ_RS03630 at 676.907 kb on - strand, within MMJJ_RS03630 at 676.907 kb on - strand, within MMJJ_RS03630 at 676.908 kb on + strand, within MMJJ_RS03630 at 676.949 kb on + strand, within MMJJ_RS03630 at 676.953 kb on - strand at 676.953 kb on - strand at 676.954 kb on + strand at 676.972 kb on - strand at 676.988 kb on - strand at 677.013 kb on - strand at 677.013 kb on - strand at 677.014 kb on + strand at 677.076 kb on + strand at 677.097 kb on + strand at 677.097 kb on + strand at 677.121 kb on + strand at 677.121 kb on + strand at 677.121 kb on + strand at 677.132 kb on - strand at 677.133 kb on + strand at 677.140 kb on - strand at 677.155 kb on - strand at 677.155 kb on - strand at 677.156 kb on + strand at 677.156 kb on + strand at 677.158 kb on + strand at 677.161 kb on - strand at 677.162 kb on + strand at 677.167 kb on + strand at 677.236 kb on + strand at 677.288 kb on - strand at 677.293 kb on - strand at 677.541 kb on - strand, within MMJJ_RS03635 at 677.554 kb on - strand, within MMJJ_RS03635 at 677.555 kb on + strand, within MMJJ_RS03635 at 677.623 kb on - strand, within MMJJ_RS03635 at 677.682 kb on + strand, within MMJJ_RS03635 at 677.720 kb on - strand, within MMJJ_RS03635
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 674,590 - MMJJ_RS03620 0.78 -2.1 674,590 - MMJJ_RS03620 0.78 -1.6 674,591 + MMJJ_RS03620 0.78 -2.3 674,638 - -3.8 674,639 + -1.5 674,655 + -2.9 674,669 - -0.0 674,688 - -0.2 674,689 + -0.2 674,689 + +0.1 674,689 + -2.4 674,692 - +0.5 674,735 + -1.9 674,741 + +0.1 674,743 - +0.3 674,744 + -0.1 674,745 - +0.5 674,745 - -0.1 674,746 + +0.4 674,746 + -0.1 674,782 - MMJJ_RS03625 0.14 -0.4 674,782 - MMJJ_RS03625 0.14 -0.2 674,782 - MMJJ_RS03625 0.14 -0.7 674,783 + MMJJ_RS03625 0.14 -0.0 674,783 + MMJJ_RS03625 0.14 -0.3 674,783 + MMJJ_RS03625 0.14 +0.1 674,821 - MMJJ_RS03625 0.19 +0.3 674,822 + MMJJ_RS03625 0.19 -2.0 674,825 + MMJJ_RS03625 0.19 -0.5 674,832 + MMJJ_RS03625 0.20 -0.0 674,888 - MMJJ_RS03625 0.27 +0.3 674,888 - MMJJ_RS03625 0.27 +0.2 674,890 - MMJJ_RS03625 0.28 -1.2 674,891 + MMJJ_RS03625 0.28 -0.1 674,923 - MMJJ_RS03625 0.32 -0.2 674,923 - MMJJ_RS03625 0.32 +1.5 674,939 + MMJJ_RS03625 0.34 -0.4 674,945 - MMJJ_RS03625 0.34 -0.8 674,946 + MMJJ_RS03625 0.34 -0.4 674,954 + MMJJ_RS03625 0.35 -0.1 674,994 + MMJJ_RS03625 0.40 -0.3 675,005 - MMJJ_RS03625 0.42 -0.3 675,006 + MMJJ_RS03625 0.42 +0.6 675,006 + MMJJ_RS03625 0.42 -0.6 675,008 - MMJJ_RS03625 0.42 +0.1 675,009 + MMJJ_RS03625 0.42 +0.2 675,013 - MMJJ_RS03625 0.43 +0.2 675,013 - MMJJ_RS03625 0.43 +0.3 675,014 + MMJJ_RS03625 0.43 +0.4 675,035 - MMJJ_RS03625 0.45 -0.3 675,036 + MMJJ_RS03625 0.46 -0.1 675,086 - MMJJ_RS03625 0.52 +0.4 675,087 + MMJJ_RS03625 0.52 -0.3 675,100 - MMJJ_RS03625 0.54 +0.3 675,100 - MMJJ_RS03625 0.54 +0.3 675,113 + MMJJ_RS03625 0.55 +0.1 675,121 - MMJJ_RS03625 0.56 -0.1 675,125 - MMJJ_RS03625 0.57 -0.0 675,126 + MMJJ_RS03625 0.57 +0.3 675,126 + MMJJ_RS03625 0.57 -0.6 675,129 + MMJJ_RS03625 0.57 +0.2 675,129 + MMJJ_RS03625 0.57 -0.1 675,156 - MMJJ_RS03625 0.60 -0.1 675,157 + MMJJ_RS03625 0.61 -0.7 675,160 - MMJJ_RS03625 0.61 +0.1 675,177 + MMJJ_RS03625 0.63 -0.5 675,177 + MMJJ_RS03625 0.63 -0.5 675,197 + MMJJ_RS03625 0.66 +0.4 675,216 + MMJJ_RS03625 0.68 -0.6 675,278 - MMJJ_RS03625 0.76 -0.7 675,278 - MMJJ_RS03625 0.76 +0.0 675,288 - MMJJ_RS03625 0.77 -0.8 675,289 + MMJJ_RS03625 0.77 +0.1 675,289 + MMJJ_RS03625 0.77 +0.3 675,394 - -1.4 675,400 + -0.2 675,429 - +0.7 675,429 - +0.4 675,429 - -0.1 675,429 - -0.1 675,429 - -0.3 675,429 - +0.2 675,429 - +0.3 675,429 - -0.1 675,430 + +0.6 675,430 + +1.1 675,431 - -0.1 675,431 - -0.9 675,431 - +0.2 675,431 - +1.2 675,431 - +0.2 675,432 + +0.5 675,490 + -1.1 675,496 + -0.1 675,504 - -0.8 675,536 + +0.6 675,539 - +0.6 675,546 + -0.3 675,552 + -0.1 675,555 - -0.4 675,561 - +1.3 675,562 + -0.2 675,565 - +0.7 675,565 - -0.4 675,565 - +0.7 675,565 - +0.9 675,565 - +0.1 675,565 - -0.3 675,565 - +0.4 675,566 + +0.3 675,566 + -3.1 675,566 + +0.5 675,566 + +0.1 675,566 + +0.3 675,566 + +0.2 675,566 + +0.1 675,568 + +0.4 675,568 + +0.9 675,596 - -1.4 675,597 + +0.4 675,597 + +0.7 675,633 - -1.7 675,634 + -2.0 675,653 - -1.9 675,688 + -0.2 675,694 - -2.9 675,694 - -2.9 675,695 + -1.8 675,695 + -2.7 675,696 - -2.1 675,696 - -1.7 675,716 + -2.1 675,743 - MMJJ_RS03630 0.10 -1.7 675,808 - MMJJ_RS03630 0.15 -1.5 675,809 + MMJJ_RS03630 0.15 -2.1 675,813 + MMJJ_RS03630 0.15 -1.3 675,814 - MMJJ_RS03630 0.15 -1.4 675,833 - MMJJ_RS03630 0.16 -1.8 675,950 - MMJJ_RS03630 0.24 -0.8 676,140 - MMJJ_RS03630 0.37 -2.1 676,141 + MMJJ_RS03630 0.37 -1.3 676,141 + MMJJ_RS03630 0.37 -4.5 676,146 + MMJJ_RS03630 0.37 -1.5 676,255 - MMJJ_RS03630 0.44 -2.3 676,269 - MMJJ_RS03630 0.45 -1.4 676,269 - MMJJ_RS03630 0.45 -2.7 676,478 - MMJJ_RS03630 0.59 -2.1 676,479 + MMJJ_RS03630 0.59 -2.4 676,479 + MMJJ_RS03630 0.59 -1.3 676,554 + MMJJ_RS03630 0.64 -1.1 676,576 + MMJJ_RS03630 0.65 -1.5 676,576 + MMJJ_RS03630 0.65 -2.7 676,600 - MMJJ_RS03630 0.67 -1.1 676,680 + MMJJ_RS03630 0.72 -2.6 676,680 + MMJJ_RS03630 0.72 -1.5 676,703 + MMJJ_RS03630 0.74 -1.9 676,707 - MMJJ_RS03630 0.74 -2.5 676,707 - MMJJ_RS03630 0.74 -2.5 676,708 + MMJJ_RS03630 0.74 -2.8 676,708 + MMJJ_RS03630 0.74 -1.6 676,711 - MMJJ_RS03630 0.74 -1.1 676,711 - MMJJ_RS03630 0.74 -3.5 676,711 - MMJJ_RS03630 0.74 -2.7 676,712 + MMJJ_RS03630 0.74 -2.7 676,781 - MMJJ_RS03630 0.79 -2.7 676,781 - MMJJ_RS03630 0.79 -0.7 676,796 - MMJJ_RS03630 0.80 -2.0 676,825 - MMJJ_RS03630 0.82 -2.5 676,859 - MMJJ_RS03630 0.84 -0.6 676,860 + MMJJ_RS03630 0.84 -2.9 676,907 - MMJJ_RS03630 0.87 +0.1 676,907 - MMJJ_RS03630 0.87 -2.0 676,908 + MMJJ_RS03630 0.87 -1.6 676,949 + MMJJ_RS03630 0.90 -2.2 676,953 - -1.4 676,953 - -2.6 676,954 + -1.7 676,972 - -2.7 676,988 - -0.0 677,013 - -1.6 677,013 - -2.2 677,014 + -0.9 677,076 + -1.1 677,097 + -1.9 677,097 + -1.3 677,121 + -1.3 677,121 + -2.3 677,121 + -1.4 677,132 - -1.6 677,133 + -0.7 677,140 - -1.3 677,155 - -2.3 677,155 - -2.6 677,156 + -1.1 677,156 + -1.4 677,158 + -1.1 677,161 - -1.5 677,162 + -1.6 677,167 + -1.1 677,236 + +0.4 677,288 - -0.3 677,293 - -1.7 677,541 - MMJJ_RS03635 0.15 -0.5 677,554 - MMJJ_RS03635 0.16 +0.2 677,555 + MMJJ_RS03635 0.16 +0.1 677,623 - MMJJ_RS03635 0.19 -0.9 677,682 + MMJJ_RS03635 0.22 -1.9 677,720 - MMJJ_RS03635 0.24 -0.9
Or see this region's nucleotide sequence