Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03280 and MMJJ_RS03285 are separated by 4 nucleotides MMJJ_RS03285 and MMJJ_RS03290 are separated by 5 nucleotides MMJJ_RS03290 and MMJJ_RS03295 are separated by 28 nucleotides MMJJ_RS03295 and MMJJ_RS03300 are separated by 5 nucleotides
MMJJ_RS03280: MMJJ_RS03280 - DNA-directed DNA polymerase, at 620,276 to 622,630
_RS03280
MMJJ_RS03285: MMJJ_RS03285 - gamma-glutamylcyclotransferase, at 622,635 to 622,958
_RS03285
MMJJ_RS03290: MMJJ_RS03290 - amidohydrolase, at 622,964 to 624,244
_RS03290
MMJJ_RS03295: MMJJ_RS03295 - PHP domain-containing protein, at 624,273 to 624,917
_RS03295
MMJJ_RS03300: MMJJ_RS03300 - RNA ligase, at 624,923 to 626,107
_RS03300
Position (kb) 622
623
624
625 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 622.031 kb on + strand, within MMJJ_RS03280 at 622.036 kb on - strand, within MMJJ_RS03280 at 622.036 kb on - strand, within MMJJ_RS03280 at 622.037 kb on + strand, within MMJJ_RS03280 at 622.037 kb on + strand, within MMJJ_RS03280 at 622.037 kb on + strand, within MMJJ_RS03280 at 622.037 kb on + strand, within MMJJ_RS03280 at 622.037 kb on + strand, within MMJJ_RS03280 at 622.039 kb on - strand, within MMJJ_RS03280 at 622.098 kb on - strand, within MMJJ_RS03280 at 622.112 kb on - strand, within MMJJ_RS03280 at 622.121 kb on + strand, within MMJJ_RS03280 at 622.146 kb on - strand, within MMJJ_RS03280 at 622.196 kb on + strand, within MMJJ_RS03280 at 622.235 kb on + strand, within MMJJ_RS03280 at 622.268 kb on + strand, within MMJJ_RS03280 at 622.321 kb on - strand, within MMJJ_RS03280 at 622.349 kb on - strand, within MMJJ_RS03280 at 622.350 kb on + strand, within MMJJ_RS03280 at 622.351 kb on - strand, within MMJJ_RS03280 at 622.352 kb on + strand, within MMJJ_RS03280 at 622.433 kb on - strand at 622.433 kb on - strand at 622.433 kb on - strand at 622.433 kb on - strand at 622.433 kb on - strand at 622.434 kb on + strand at 622.434 kb on + strand at 622.441 kb on + strand at 622.512 kb on + strand at 622.512 kb on + strand at 622.514 kb on - strand at 622.514 kb on - strand at 622.515 kb on + strand at 622.544 kb on + strand at 622.545 kb on - strand at 622.571 kb on + strand at 622.604 kb on - strand at 622.697 kb on + strand, within MMJJ_RS03285 at 622.697 kb on + strand, within MMJJ_RS03285 at 622.733 kb on + strand, within MMJJ_RS03285 at 622.737 kb on + strand, within MMJJ_RS03285 at 622.878 kb on - strand, within MMJJ_RS03285 at 622.878 kb on - strand, within MMJJ_RS03285 at 622.878 kb on - strand, within MMJJ_RS03285 at 622.879 kb on + strand, within MMJJ_RS03285 at 622.879 kb on + strand, within MMJJ_RS03285 at 622.879 kb on + strand, within MMJJ_RS03285 at 622.879 kb on + strand, within MMJJ_RS03285 at 622.879 kb on + strand, within MMJJ_RS03285 at 622.879 kb on + strand, within MMJJ_RS03285 at 622.913 kb on + strand, within MMJJ_RS03285 at 622.913 kb on + strand, within MMJJ_RS03285 at 622.927 kb on + strand at 622.935 kb on - strand at 622.945 kb on - strand at 622.946 kb on + strand at 622.968 kb on - strand at 622.995 kb on - strand at 622.995 kb on - strand at 622.999 kb on - strand at 622.999 kb on - strand at 623.000 kb on + strand at 623.000 kb on + strand at 623.002 kb on - strand at 623.002 kb on - strand at 623.007 kb on - strand at 623.008 kb on + strand at 623.035 kb on + strand at 623.064 kb on - strand at 623.065 kb on + strand at 623.159 kb on - strand, within MMJJ_RS03290 at 623.160 kb on + strand, within MMJJ_RS03290 at 623.282 kb on - strand at 623.348 kb on - strand, within MMJJ_RS03290 at 623.377 kb on - strand, within MMJJ_RS03290 at 623.377 kb on - strand, within MMJJ_RS03290 at 623.406 kb on - strand, within MMJJ_RS03290 at 623.411 kb on - strand, within MMJJ_RS03290 at 623.422 kb on + strand, within MMJJ_RS03290 at 623.449 kb on - strand, within MMJJ_RS03290 at 623.450 kb on + strand, within MMJJ_RS03290 at 623.451 kb on - strand, within MMJJ_RS03290 at 623.452 kb on + strand, within MMJJ_RS03290 at 623.452 kb on + strand, within MMJJ_RS03290 at 623.452 kb on + strand, within MMJJ_RS03290 at 623.452 kb on + strand, within MMJJ_RS03290 at 623.456 kb on + strand, within MMJJ_RS03290 at 623.456 kb on + strand, within MMJJ_RS03290 at 623.463 kb on - strand, within MMJJ_RS03290 at 623.511 kb on - strand, within MMJJ_RS03290 at 623.518 kb on + strand, within MMJJ_RS03290 at 623.541 kb on + strand, within MMJJ_RS03290 at 623.591 kb on - strand, within MMJJ_RS03290 at 623.592 kb on + strand, within MMJJ_RS03290 at 623.592 kb on + strand, within MMJJ_RS03290 at 623.598 kb on - strand, within MMJJ_RS03290 at 623.598 kb on - strand, within MMJJ_RS03290 at 623.598 kb on - strand, within MMJJ_RS03290 at 623.599 kb on + strand, within MMJJ_RS03290 at 623.599 kb on + strand, within MMJJ_RS03290 at 623.599 kb on + strand, within MMJJ_RS03290 at 623.599 kb on + strand, within MMJJ_RS03290 at 623.602 kb on - strand, within MMJJ_RS03290 at 623.603 kb on + strand, within MMJJ_RS03290 at 623.603 kb on + strand, within MMJJ_RS03290 at 623.637 kb on - strand, within MMJJ_RS03290 at 623.638 kb on + strand, within MMJJ_RS03290 at 623.673 kb on - strand, within MMJJ_RS03290 at 623.678 kb on - strand, within MMJJ_RS03290 at 623.679 kb on + strand, within MMJJ_RS03290 at 623.679 kb on + strand, within MMJJ_RS03290 at 623.679 kb on + strand, within MMJJ_RS03290 at 623.826 kb on - strand, within MMJJ_RS03290 at 623.826 kb on - strand, within MMJJ_RS03290 at 623.827 kb on + strand, within MMJJ_RS03290 at 623.827 kb on + strand, within MMJJ_RS03290 at 623.852 kb on - strand, within MMJJ_RS03290 at 623.899 kb on - strand, within MMJJ_RS03290 at 623.899 kb on - strand, within MMJJ_RS03290 at 623.909 kb on + strand, within MMJJ_RS03290 at 623.914 kb on - strand, within MMJJ_RS03290 at 623.940 kb on - strand, within MMJJ_RS03290 at 623.940 kb on - strand, within MMJJ_RS03290 at 623.944 kb on - strand, within MMJJ_RS03290 at 623.945 kb on + strand, within MMJJ_RS03290 at 624.010 kb on - strand, within MMJJ_RS03290 at 624.038 kb on + strand, within MMJJ_RS03290 at 624.040 kb on - strand, within MMJJ_RS03290 at 624.070 kb on + strand, within MMJJ_RS03290 at 624.082 kb on - strand, within MMJJ_RS03290 at 624.085 kb on - strand, within MMJJ_RS03290 at 624.087 kb on - strand, within MMJJ_RS03290 at 624.102 kb on - strand, within MMJJ_RS03290 at 624.102 kb on - strand, within MMJJ_RS03290 at 624.102 kb on - strand, within MMJJ_RS03290 at 624.103 kb on + strand, within MMJJ_RS03290 at 624.103 kb on + strand, within MMJJ_RS03290 at 624.103 kb on + strand, within MMJJ_RS03290 at 624.135 kb on - strand at 624.138 kb on - strand at 624.151 kb on - strand at 624.151 kb on - strand at 624.151 kb on - strand at 624.152 kb on + strand at 624.195 kb on - strand at 624.195 kb on - strand at 624.195 kb on - strand at 624.196 kb on + strand at 624.196 kb on + strand at 624.199 kb on - strand at 624.199 kb on - strand at 624.199 kb on - strand at 624.200 kb on + strand at 624.202 kb on - strand at 624.214 kb on - strand at 624.214 kb on - strand at 624.214 kb on - strand at 624.215 kb on + strand at 624.295 kb on - strand at 624.295 kb on - strand at 624.295 kb on - strand at 624.295 kb on - strand at 624.296 kb on + strand at 624.419 kb on + strand, within MMJJ_RS03295 at 624.496 kb on - strand, within MMJJ_RS03295 at 624.540 kb on - strand, within MMJJ_RS03295 at 624.541 kb on + strand, within MMJJ_RS03295 at 624.584 kb on - strand, within MMJJ_RS03295 at 624.584 kb on - strand, within MMJJ_RS03295 at 624.616 kb on - strand, within MMJJ_RS03295 at 624.617 kb on + strand, within MMJJ_RS03295 at 624.631 kb on - strand, within MMJJ_RS03295 at 624.631 kb on - strand, within MMJJ_RS03295 at 624.631 kb on - strand, within MMJJ_RS03295 at 624.632 kb on + strand, within MMJJ_RS03295 at 624.632 kb on + strand, within MMJJ_RS03295 at 624.632 kb on + strand, within MMJJ_RS03295 at 624.640 kb on - strand, within MMJJ_RS03295 at 624.641 kb on + strand, within MMJJ_RS03295 at 624.641 kb on + strand, within MMJJ_RS03295 at 624.641 kb on + strand, within MMJJ_RS03295 at 624.641 kb on + strand, within MMJJ_RS03295 at 624.641 kb on + strand, within MMJJ_RS03295 at 624.642 kb on - strand, within MMJJ_RS03295 at 624.642 kb on - strand, within MMJJ_RS03295 at 624.643 kb on + strand, within MMJJ_RS03295 at 624.643 kb on + strand, within MMJJ_RS03295 at 624.644 kb on - strand, within MMJJ_RS03295 at 624.684 kb on - strand, within MMJJ_RS03295 at 624.685 kb on + strand, within MMJJ_RS03295 at 624.701 kb on - strand, within MMJJ_RS03295 at 624.702 kb on + strand, within MMJJ_RS03295 at 624.752 kb on + strand, within MMJJ_RS03295 at 624.762 kb on - strand, within MMJJ_RS03295 at 624.787 kb on - strand, within MMJJ_RS03295 at 624.788 kb on + strand, within MMJJ_RS03295 at 624.792 kb on + strand, within MMJJ_RS03295 at 624.844 kb on + strand, within MMJJ_RS03295 at 624.844 kb on + strand, within MMJJ_RS03295 at 624.877 kb on - strand at 624.877 kb on - strand at 624.877 kb on - strand at 624.877 kb on - strand at 624.877 kb on - strand at 624.878 kb on + strand at 624.878 kb on + strand at 624.878 kb on + strand at 624.878 kb on + strand at 624.878 kb on + strand at 624.884 kb on - strand at 624.885 kb on + strand at 624.910 kb on - strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.949 kb on - strand at 624.975 kb on - strand at 624.976 kb on + strand at 625.046 kb on + strand, within MMJJ_RS03300 at 625.144 kb on - strand, within MMJJ_RS03300 at 625.147 kb on - strand, within MMJJ_RS03300 at 625.203 kb on + strand, within MMJJ_RS03300 at 625.244 kb on - strand, within MMJJ_RS03300
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 622,031 + MMJJ_RS03280 0.75 +0.9 622,036 - MMJJ_RS03280 0.75 -0.2 622,036 - MMJJ_RS03280 0.75 -0.4 622,037 + MMJJ_RS03280 0.75 -0.8 622,037 + MMJJ_RS03280 0.75 +0.5 622,037 + MMJJ_RS03280 0.75 -0.0 622,037 + MMJJ_RS03280 0.75 +0.5 622,037 + MMJJ_RS03280 0.75 -0.8 622,039 - MMJJ_RS03280 0.75 -0.4 622,098 - MMJJ_RS03280 0.77 -1.0 622,112 - MMJJ_RS03280 0.78 +0.1 622,121 + MMJJ_RS03280 0.78 -0.4 622,146 - MMJJ_RS03280 0.79 -0.3 622,196 + MMJJ_RS03280 0.82 +0.3 622,235 + MMJJ_RS03280 0.83 +0.8 622,268 + MMJJ_RS03280 0.85 +0.0 622,321 - MMJJ_RS03280 0.87 -0.5 622,349 - MMJJ_RS03280 0.88 -0.3 622,350 + MMJJ_RS03280 0.88 -0.5 622,351 - MMJJ_RS03280 0.88 -0.3 622,352 + MMJJ_RS03280 0.88 +0.8 622,433 - -0.2 622,433 - +0.5 622,433 - +0.6 622,433 - -0.4 622,433 - -0.0 622,434 + +1.0 622,434 + -0.0 622,441 + -0.4 622,512 + -0.7 622,512 + +0.4 622,514 - +1.0 622,514 - -1.2 622,515 + -0.4 622,544 + -0.5 622,545 - -0.1 622,571 + +0.6 622,604 - +1.6 622,697 + MMJJ_RS03285 0.19 -0.0 622,697 + MMJJ_RS03285 0.19 +0.4 622,733 + MMJJ_RS03285 0.30 +0.2 622,737 + MMJJ_RS03285 0.31 +0.5 622,878 - MMJJ_RS03285 0.75 +0.6 622,878 - MMJJ_RS03285 0.75 +0.6 622,878 - MMJJ_RS03285 0.75 +0.3 622,879 + MMJJ_RS03285 0.75 +1.2 622,879 + MMJJ_RS03285 0.75 +0.3 622,879 + MMJJ_RS03285 0.75 +0.3 622,879 + MMJJ_RS03285 0.75 +1.1 622,879 + MMJJ_RS03285 0.75 -0.6 622,879 + MMJJ_RS03285 0.75 -0.1 622,913 + MMJJ_RS03285 0.86 -0.6 622,913 + MMJJ_RS03285 0.86 -1.1 622,927 + -0.8 622,935 - -0.9 622,945 - +0.6 622,946 + -0.5 622,968 - +1.3 622,995 - -1.1 622,995 - +0.7 622,999 - +0.7 622,999 - +0.1 623,000 + -0.3 623,000 + -0.7 623,002 - -0.3 623,002 - -0.3 623,007 - -0.8 623,008 + -0.7 623,035 + +0.5 623,064 - +0.2 623,065 + +0.3 623,159 - MMJJ_RS03290 0.15 -0.1 623,160 + MMJJ_RS03290 0.15 -0.1 623,282 - +0.5 623,348 - MMJJ_RS03290 0.30 +0.5 623,377 - MMJJ_RS03290 0.32 +0.4 623,377 - MMJJ_RS03290 0.32 -1.2 623,406 - MMJJ_RS03290 0.35 +0.4 623,411 - MMJJ_RS03290 0.35 +1.2 623,422 + MMJJ_RS03290 0.36 +0.2 623,449 - MMJJ_RS03290 0.38 +0.3 623,450 + MMJJ_RS03290 0.38 +0.4 623,451 - MMJJ_RS03290 0.38 +0.2 623,452 + MMJJ_RS03290 0.38 -0.0 623,452 + MMJJ_RS03290 0.38 -0.3 623,452 + MMJJ_RS03290 0.38 +0.4 623,452 + MMJJ_RS03290 0.38 -0.8 623,456 + MMJJ_RS03290 0.38 -0.3 623,456 + MMJJ_RS03290 0.38 +0.5 623,463 - MMJJ_RS03290 0.39 -0.5 623,511 - MMJJ_RS03290 0.43 -0.2 623,518 + MMJJ_RS03290 0.43 -0.0 623,541 + MMJJ_RS03290 0.45 +1.0 623,591 - MMJJ_RS03290 0.49 +1.0 623,592 + MMJJ_RS03290 0.49 +0.1 623,592 + MMJJ_RS03290 0.49 -0.3 623,598 - MMJJ_RS03290 0.49 -0.4 623,598 - MMJJ_RS03290 0.49 +0.6 623,598 - MMJJ_RS03290 0.49 -0.6 623,599 + MMJJ_RS03290 0.50 +1.1 623,599 + MMJJ_RS03290 0.50 +0.6 623,599 + MMJJ_RS03290 0.50 +0.7 623,599 + MMJJ_RS03290 0.50 +0.9 623,602 - MMJJ_RS03290 0.50 +1.0 623,603 + MMJJ_RS03290 0.50 +0.8 623,603 + MMJJ_RS03290 0.50 -0.2 623,637 - MMJJ_RS03290 0.53 +0.9 623,638 + MMJJ_RS03290 0.53 -0.0 623,673 - MMJJ_RS03290 0.55 -0.4 623,678 - MMJJ_RS03290 0.56 -1.3 623,679 + MMJJ_RS03290 0.56 -0.3 623,679 + MMJJ_RS03290 0.56 -0.1 623,679 + MMJJ_RS03290 0.56 -0.0 623,826 - MMJJ_RS03290 0.67 +0.2 623,826 - MMJJ_RS03290 0.67 +0.9 623,827 + MMJJ_RS03290 0.67 +1.0 623,827 + MMJJ_RS03290 0.67 +0.4 623,852 - MMJJ_RS03290 0.69 +0.3 623,899 - MMJJ_RS03290 0.73 +0.1 623,899 - MMJJ_RS03290 0.73 +0.2 623,909 + MMJJ_RS03290 0.74 -1.0 623,914 - MMJJ_RS03290 0.74 +0.6 623,940 - MMJJ_RS03290 0.76 +1.1 623,940 - MMJJ_RS03290 0.76 +0.2 623,944 - MMJJ_RS03290 0.77 +0.0 623,945 + MMJJ_RS03290 0.77 -0.0 624,010 - MMJJ_RS03290 0.82 +0.4 624,038 + MMJJ_RS03290 0.84 +1.2 624,040 - MMJJ_RS03290 0.84 +0.1 624,070 + MMJJ_RS03290 0.86 +0.1 624,082 - MMJJ_RS03290 0.87 +0.2 624,085 - MMJJ_RS03290 0.88 +0.3 624,087 - MMJJ_RS03290 0.88 -0.3 624,102 - MMJJ_RS03290 0.89 -1.0 624,102 - MMJJ_RS03290 0.89 -1.1 624,102 - MMJJ_RS03290 0.89 +0.3 624,103 + MMJJ_RS03290 0.89 +0.0 624,103 + MMJJ_RS03290 0.89 -0.4 624,103 + MMJJ_RS03290 0.89 +0.5 624,135 - -0.2 624,138 - +0.3 624,151 - -0.1 624,151 - +1.5 624,151 - +0.0 624,152 + +0.3 624,195 - -2.5 624,195 - -0.5 624,195 - -0.8 624,196 + -1.4 624,196 + -2.1 624,199 - -1.2 624,199 - -0.1 624,199 - -0.2 624,200 + -0.4 624,202 - +1.9 624,214 - -0.5 624,214 - -0.1 624,214 - +0.1 624,215 + -1.4 624,295 - -2.2 624,295 - -1.8 624,295 - -2.1 624,295 - -1.9 624,296 + -2.3 624,419 + MMJJ_RS03295 0.23 -1.5 624,496 - MMJJ_RS03295 0.35 -0.9 624,540 - MMJJ_RS03295 0.41 -2.2 624,541 + MMJJ_RS03295 0.42 -0.8 624,584 - MMJJ_RS03295 0.48 -1.5 624,584 - MMJJ_RS03295 0.48 -2.2 624,616 - MMJJ_RS03295 0.53 -2.2 624,617 + MMJJ_RS03295 0.53 -1.7 624,631 - MMJJ_RS03295 0.56 -1.8 624,631 - MMJJ_RS03295 0.56 -2.1 624,631 - MMJJ_RS03295 0.56 -2.0 624,632 + MMJJ_RS03295 0.56 -1.2 624,632 + MMJJ_RS03295 0.56 -1.9 624,632 + MMJJ_RS03295 0.56 -1.6 624,640 - MMJJ_RS03295 0.57 -1.9 624,641 + MMJJ_RS03295 0.57 -1.5 624,641 + MMJJ_RS03295 0.57 -3.0 624,641 + MMJJ_RS03295 0.57 -1.3 624,641 + MMJJ_RS03295 0.57 -2.0 624,641 + MMJJ_RS03295 0.57 -1.8 624,642 - MMJJ_RS03295 0.57 -2.8 624,642 - MMJJ_RS03295 0.57 -2.4 624,643 + MMJJ_RS03295 0.57 -2.1 624,643 + MMJJ_RS03295 0.57 -3.8 624,644 - MMJJ_RS03295 0.58 -3.3 624,684 - MMJJ_RS03295 0.64 -2.9 624,685 + MMJJ_RS03295 0.64 -1.7 624,701 - MMJJ_RS03295 0.66 -1.4 624,702 + MMJJ_RS03295 0.67 -1.8 624,752 + MMJJ_RS03295 0.74 -1.7 624,762 - MMJJ_RS03295 0.76 -1.9 624,787 - MMJJ_RS03295 0.80 -3.0 624,788 + MMJJ_RS03295 0.80 -3.9 624,792 + MMJJ_RS03295 0.80 -2.1 624,844 + MMJJ_RS03295 0.89 -1.5 624,844 + MMJJ_RS03295 0.89 -1.8 624,877 - -1.5 624,877 - -2.2 624,877 - -3.1 624,877 - -1.4 624,877 - -3.6 624,878 + -2.5 624,878 + -2.0 624,878 + -0.6 624,878 + -1.6 624,878 + -3.7 624,884 - -1.9 624,885 + +0.1 624,910 - -0.3 624,936 + -0.4 624,936 + +0.4 624,949 - -0.2 624,975 - +0.1 624,976 + +0.1 625,046 + MMJJ_RS03300 0.10 +0.6 625,144 - MMJJ_RS03300 0.19 -3.3 625,147 - MMJJ_RS03300 0.19 -0.3 625,203 + MMJJ_RS03300 0.24 -0.8 625,244 - MMJJ_RS03300 0.27 +0.0
Or see this region's nucleotide sequence