Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03110 and MMJJ_RS03115 are separated by 35 nucleotides MMJJ_RS03115 and MMJJ_RS03120 are separated by 67 nucleotides
MMJJ_RS03110: MMJJ_RS03110 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase, at 587,345 to 588,622
_RS03110
MMJJ_RS03115: MMJJ_RS03115 - sulfopyruvate decarboxylase subunit alpha, at 588,658 to 589,161
_RS03115
MMJJ_RS03120: MMJJ_RS03120 - ribose-phosphate diphosphokinase, at 589,229 to 590,092
_RS03120
Position (kb) 588
589
590 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 587.737 kb on - strand, within MMJJ_RS03110 at 588.072 kb on + strand, within MMJJ_RS03110 at 588.150 kb on - strand, within MMJJ_RS03110 at 588.151 kb on + strand, within MMJJ_RS03110 at 588.154 kb on - strand, within MMJJ_RS03110 at 588.179 kb on - strand, within MMJJ_RS03110 at 588.405 kb on + strand, within MMJJ_RS03110 at 588.487 kb on - strand at 588.488 kb on + strand, within MMJJ_RS03110 at 588.500 kb on - strand at 588.501 kb on + strand at 588.501 kb on + strand at 588.689 kb on - strand at 588.689 kb on - strand at 588.690 kb on + strand at 588.692 kb on - strand at 588.692 kb on - strand at 588.758 kb on - strand, within MMJJ_RS03115 at 588.765 kb on - strand, within MMJJ_RS03115 at 588.939 kb on - strand, within MMJJ_RS03115 at 588.941 kb on - strand, within MMJJ_RS03115 at 588.944 kb on - strand, within MMJJ_RS03115 at 588.944 kb on - strand, within MMJJ_RS03115 at 588.944 kb on - strand, within MMJJ_RS03115 at 588.945 kb on + strand, within MMJJ_RS03115 at 588.945 kb on + strand at 589.159 kb on - strand at 589.169 kb on - strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.191 kb on - strand at 589.226 kb on - strand at 589.227 kb on + strand at 589.230 kb on - strand at 589.241 kb on - strand at 589.244 kb on - strand at 589.244 kb on - strand at 589.244 kb on - strand at 589.244 kb on - strand at 589.245 kb on + strand at 589.272 kb on + strand at 589.485 kb on + strand at 589.485 kb on + strand at 589.844 kb on - strand, within MMJJ_RS03120 at 590.126 kb on - strand at 590.147 kb on - strand at 590.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 587,737 - MMJJ_RS03110 0.31 -0.6 588,072 + MMJJ_RS03110 0.57 -1.7 588,150 - MMJJ_RS03110 0.63 -2.8 588,151 + MMJJ_RS03110 0.63 -2.6 588,154 - MMJJ_RS03110 0.63 -0.7 588,179 - MMJJ_RS03110 0.65 -2.6 588,405 + MMJJ_RS03110 0.83 -2.8 588,487 - -0.1 588,488 + MMJJ_RS03110 0.89 -1.6 588,500 - -0.3 588,501 + -1.1 588,501 + -0.1 588,689 - -1.0 588,689 - -0.8 588,690 + -0.9 588,692 - -0.4 588,692 - -0.5 588,758 - MMJJ_RS03115 0.20 -1.9 588,765 - MMJJ_RS03115 0.21 -1.7 588,939 - MMJJ_RS03115 0.56 -2.0 588,941 - MMJJ_RS03115 0.56 -0.8 588,944 - MMJJ_RS03115 0.57 -2.8 588,944 - MMJJ_RS03115 0.57 -0.1 588,944 - MMJJ_RS03115 0.57 -2.0 588,945 + MMJJ_RS03115 0.57 -0.2 588,945 + -1.4 589,159 - -1.4 589,169 - -0.9 589,173 + +0.1 589,173 + -1.7 589,173 + -1.3 589,173 + -1.4 589,191 - -1.4 589,226 - -0.7 589,227 + -1.7 589,230 - -1.4 589,241 - -0.2 589,244 - -2.4 589,244 - -1.1 589,244 - -1.5 589,244 - -0.7 589,245 + -3.6 589,272 + +0.5 589,485 + -1.4 589,485 + -1.4 589,844 - MMJJ_RS03120 0.71 -2.9 590,126 - -0.9 590,147 - -3.9 590,147 - -0.8
Or see this region's nucleotide sequence