Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03030 and MMJJ_RS03035 are separated by 251 nucleotides MMJJ_RS03035 and MMJJ_RS03040 are separated by 13 nucleotides MMJJ_RS03040 and MMJJ_RS03045 are separated by 410 nucleotides MMJJ_RS03045 and MMJJ_RS03050 are separated by 156 nucleotides
MMJJ_RS03030: MMJJ_RS03030 - hypothetical protein, at 569,593 to 570,672
_RS03030
MMJJ_RS03035: MMJJ_RS03035 - nitroreductase family protein, at 570,924 to 571,493
_RS03035
MMJJ_RS03040: MMJJ_RS03040 - lactate utilization protein, at 571,507 to 572,148
_RS03040
MMJJ_RS03045: MMJJ_RS03045 - hypothetical protein, at 572,559 to 572,774
_RS03045
MMJJ_RS03050: MMJJ_RS03050 - hypothetical protein, at 572,931 to 574,211
_RS03050
Position (kb) 571
572
573 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 570.517 kb on - strand, within MMJJ_RS03030 at 570.518 kb on + strand, within MMJJ_RS03030 at 570.546 kb on - strand, within MMJJ_RS03030 at 570.547 kb on + strand, within MMJJ_RS03030 at 570.547 kb on + strand, within MMJJ_RS03030 at 570.547 kb on + strand, within MMJJ_RS03030 at 570.550 kb on + strand, within MMJJ_RS03030 at 570.561 kb on - strand, within MMJJ_RS03030 at 570.565 kb on - strand at 570.566 kb on + strand at 570.566 kb on + strand at 570.588 kb on - strand at 570.605 kb on - strand at 570.643 kb on - strand at 570.644 kb on + strand at 570.644 kb on + strand at 570.692 kb on - strand at 570.692 kb on - strand at 570.699 kb on - strand at 570.708 kb on - strand at 570.720 kb on - strand at 570.723 kb on - strand at 570.747 kb on - strand at 570.773 kb on - strand at 570.773 kb on - strand at 570.773 kb on - strand at 570.773 kb on - strand at 570.774 kb on + strand at 570.774 kb on + strand at 570.774 kb on + strand at 570.774 kb on + strand at 570.774 kb on + strand at 570.778 kb on + strand at 570.778 kb on + strand at 570.783 kb on - strand at 570.784 kb on + strand at 570.789 kb on + strand at 570.820 kb on + strand at 570.820 kb on + strand at 570.820 kb on + strand at 570.831 kb on - strand at 570.832 kb on + strand at 570.834 kb on - strand at 570.838 kb on + strand at 570.839 kb on - strand at 570.839 kb on - strand at 570.858 kb on + strand at 570.863 kb on + strand at 570.911 kb on + strand at 570.926 kb on + strand at 570.931 kb on + strand at 570.935 kb on + strand at 571.009 kb on - strand, within MMJJ_RS03035 at 571.061 kb on - strand, within MMJJ_RS03035 at 571.074 kb on - strand, within MMJJ_RS03035 at 571.075 kb on + strand, within MMJJ_RS03035 at 571.176 kb on + strand, within MMJJ_RS03035 at 571.210 kb on - strand, within MMJJ_RS03035 at 571.215 kb on - strand, within MMJJ_RS03035 at 571.215 kb on - strand, within MMJJ_RS03035 at 571.215 kb on - strand, within MMJJ_RS03035 at 571.216 kb on + strand, within MMJJ_RS03035 at 571.216 kb on + strand, within MMJJ_RS03035 at 571.216 kb on + strand, within MMJJ_RS03035 at 571.220 kb on - strand, within MMJJ_RS03035 at 571.220 kb on - strand, within MMJJ_RS03035 at 571.220 kb on - strand, within MMJJ_RS03035 at 571.221 kb on + strand, within MMJJ_RS03035 at 571.221 kb on + strand, within MMJJ_RS03035 at 571.222 kb on - strand, within MMJJ_RS03035 at 571.223 kb on + strand, within MMJJ_RS03035 at 571.223 kb on + strand, within MMJJ_RS03035 at 571.306 kb on + strand, within MMJJ_RS03035 at 571.310 kb on - strand, within MMJJ_RS03035 at 571.315 kb on + strand, within MMJJ_RS03035 at 571.345 kb on + strand, within MMJJ_RS03035 at 571.345 kb on + strand, within MMJJ_RS03035 at 571.350 kb on + strand, within MMJJ_RS03035 at 571.350 kb on + strand, within MMJJ_RS03035 at 571.471 kb on + strand at 571.545 kb on - strand at 571.546 kb on + strand at 571.546 kb on + strand at 571.570 kb on + strand at 571.634 kb on + strand, within MMJJ_RS03040 at 571.719 kb on - strand, within MMJJ_RS03040 at 571.719 kb on - strand, within MMJJ_RS03040 at 571.719 kb on - strand, within MMJJ_RS03040 at 571.719 kb on - strand, within MMJJ_RS03040 at 571.729 kb on + strand, within MMJJ_RS03040 at 571.747 kb on - strand, within MMJJ_RS03040 at 571.747 kb on - strand, within MMJJ_RS03040 at 571.747 kb on - strand, within MMJJ_RS03040 at 571.807 kb on - strand, within MMJJ_RS03040 at 571.807 kb on - strand, within MMJJ_RS03040 at 571.807 kb on - strand, within MMJJ_RS03040 at 571.808 kb on + strand, within MMJJ_RS03040 at 571.827 kb on - strand, within MMJJ_RS03040 at 571.839 kb on - strand, within MMJJ_RS03040 at 571.849 kb on + strand, within MMJJ_RS03040 at 571.852 kb on - strand, within MMJJ_RS03040 at 571.852 kb on - strand, within MMJJ_RS03040 at 571.852 kb on - strand, within MMJJ_RS03040 at 571.852 kb on - strand, within MMJJ_RS03040 at 571.855 kb on + strand, within MMJJ_RS03040 at 571.864 kb on - strand, within MMJJ_RS03040 at 571.865 kb on + strand, within MMJJ_RS03040 at 571.975 kb on - strand, within MMJJ_RS03040 at 572.005 kb on + strand, within MMJJ_RS03040 at 572.005 kb on + strand, within MMJJ_RS03040 at 572.022 kb on + strand, within MMJJ_RS03040 at 572.031 kb on - strand, within MMJJ_RS03040 at 572.032 kb on + strand, within MMJJ_RS03040 at 572.033 kb on - strand, within MMJJ_RS03040 at 572.033 kb on - strand, within MMJJ_RS03040 at 572.033 kb on - strand, within MMJJ_RS03040 at 572.033 kb on - strand, within MMJJ_RS03040 at 572.033 kb on - strand, within MMJJ_RS03040 at 572.034 kb on + strand, within MMJJ_RS03040 at 572.034 kb on + strand, within MMJJ_RS03040 at 572.034 kb on + strand, within MMJJ_RS03040 at 572.034 kb on + strand, within MMJJ_RS03040 at 572.037 kb on - strand, within MMJJ_RS03040 at 572.063 kb on - strand, within MMJJ_RS03040 at 572.205 kb on + strand at 572.205 kb on + strand at 572.205 kb on + strand at 572.205 kb on + strand at 572.205 kb on + strand at 572.208 kb on - strand at 572.213 kb on - strand at 572.213 kb on - strand at 572.213 kb on - strand at 572.213 kb on - strand at 572.214 kb on + strand at 572.214 kb on + strand at 572.214 kb on + strand at 572.214 kb on + strand at 572.214 kb on + strand at 572.214 kb on + strand at 572.220 kb on + strand at 572.235 kb on - strand at 572.245 kb on - strand at 572.286 kb on - strand at 572.287 kb on + strand at 572.336 kb on - strand at 572.348 kb on - strand at 572.349 kb on + strand at 572.355 kb on - strand at 572.368 kb on + strand at 572.370 kb on - strand at 572.371 kb on + strand at 572.371 kb on + strand at 572.417 kb on - strand at 572.443 kb on + strand at 572.473 kb on - strand at 572.474 kb on + strand at 572.474 kb on + strand at 572.506 kb on + strand at 572.506 kb on + strand at 572.506 kb on + strand at 572.566 kb on - strand at 572.567 kb on + strand at 572.582 kb on - strand, within MMJJ_RS03045 at 572.583 kb on + strand, within MMJJ_RS03045 at 572.591 kb on - strand, within MMJJ_RS03045 at 572.592 kb on + strand, within MMJJ_RS03045 at 572.603 kb on - strand, within MMJJ_RS03045 at 572.604 kb on + strand, within MMJJ_RS03045 at 572.627 kb on - strand, within MMJJ_RS03045 at 572.648 kb on - strand, within MMJJ_RS03045 at 572.654 kb on - strand, within MMJJ_RS03045 at 572.655 kb on + strand, within MMJJ_RS03045 at 572.659 kb on - strand, within MMJJ_RS03045 at 572.659 kb on - strand, within MMJJ_RS03045 at 572.683 kb on - strand, within MMJJ_RS03045 at 572.687 kb on - strand, within MMJJ_RS03045 at 572.687 kb on - strand, within MMJJ_RS03045 at 572.687 kb on - strand, within MMJJ_RS03045 at 572.688 kb on + strand, within MMJJ_RS03045 at 572.688 kb on + strand, within MMJJ_RS03045 at 572.688 kb on + strand, within MMJJ_RS03045 at 572.691 kb on - strand, within MMJJ_RS03045 at 572.693 kb on - strand, within MMJJ_RS03045 at 572.694 kb on + strand, within MMJJ_RS03045 at 572.704 kb on + strand, within MMJJ_RS03045 at 572.704 kb on + strand, within MMJJ_RS03045 at 572.704 kb on + strand, within MMJJ_RS03045 at 572.712 kb on - strand, within MMJJ_RS03045 at 572.712 kb on - strand, within MMJJ_RS03045 at 572.712 kb on - strand, within MMJJ_RS03045 at 572.713 kb on + strand, within MMJJ_RS03045 at 572.713 kb on + strand, within MMJJ_RS03045 at 572.713 kb on + strand, within MMJJ_RS03045 at 572.713 kb on + strand, within MMJJ_RS03045 at 572.714 kb on - strand, within MMJJ_RS03045 at 572.717 kb on + strand, within MMJJ_RS03045 at 572.773 kb on + strand at 572.786 kb on - strand at 572.790 kb on - strand at 572.796 kb on - strand at 572.804 kb on - strand at 572.805 kb on + strand at 572.808 kb on - strand at 572.877 kb on - strand at 572.877 kb on - strand at 572.884 kb on - strand at 572.885 kb on + strand at 572.885 kb on + strand at 572.885 kb on + strand at 572.899 kb on - strand at 572.942 kb on - strand at 573.005 kb on - strand at 573.026 kb on + strand at 573.028 kb on - strand at 573.044 kb on - strand at 573.045 kb on + strand at 573.045 kb on + strand at 573.060 kb on - strand, within MMJJ_RS03050 at 573.095 kb on - strand, within MMJJ_RS03050 at 573.100 kb on + strand, within MMJJ_RS03050 at 573.100 kb on + strand, within MMJJ_RS03050 at 573.129 kb on - strand, within MMJJ_RS03050 at 573.139 kb on - strand, within MMJJ_RS03050 at 573.145 kb on - strand, within MMJJ_RS03050 at 573.146 kb on + strand, within MMJJ_RS03050
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 570,517 - MMJJ_RS03030 0.86 -0.7 570,518 + MMJJ_RS03030 0.86 -1.2 570,546 - MMJJ_RS03030 0.88 -0.6 570,547 + MMJJ_RS03030 0.88 +0.7 570,547 + MMJJ_RS03030 0.88 +0.2 570,547 + MMJJ_RS03030 0.88 -0.2 570,550 + MMJJ_RS03030 0.89 -0.7 570,561 - MMJJ_RS03030 0.90 +1.0 570,565 - -1.4 570,566 + -0.8 570,566 + +0.6 570,588 - -0.1 570,605 - +0.4 570,643 - +0.3 570,644 + -0.4 570,644 + -1.1 570,692 - +0.3 570,692 - -0.1 570,699 - +0.3 570,708 - -0.1 570,720 - -0.1 570,723 - -0.1 570,747 - +1.5 570,773 - -1.3 570,773 - -0.6 570,773 - -0.7 570,773 - +0.1 570,774 + +0.4 570,774 + +0.3 570,774 + -0.1 570,774 + -0.1 570,774 + -1.4 570,778 + -0.6 570,778 + +0.7 570,783 - +0.6 570,784 + +0.3 570,789 + -0.9 570,820 + -1.4 570,820 + -1.4 570,820 + -0.1 570,831 - +0.4 570,832 + +0.2 570,834 - -0.7 570,838 + -0.5 570,839 - +0.5 570,839 - -2.8 570,858 + -0.1 570,863 + -0.6 570,911 + -0.8 570,926 + +1.2 570,931 + +0.7 570,935 + -0.8 571,009 - MMJJ_RS03035 0.15 -0.0 571,061 - MMJJ_RS03035 0.24 +0.5 571,074 - MMJJ_RS03035 0.26 -1.4 571,075 + MMJJ_RS03035 0.26 +0.2 571,176 + MMJJ_RS03035 0.44 +0.3 571,210 - MMJJ_RS03035 0.50 +0.1 571,215 - MMJJ_RS03035 0.51 +0.9 571,215 - MMJJ_RS03035 0.51 +0.8 571,215 - MMJJ_RS03035 0.51 -0.0 571,216 + MMJJ_RS03035 0.51 +0.2 571,216 + MMJJ_RS03035 0.51 -0.3 571,216 + MMJJ_RS03035 0.51 +0.6 571,220 - MMJJ_RS03035 0.52 +0.7 571,220 - MMJJ_RS03035 0.52 -0.2 571,220 - MMJJ_RS03035 0.52 -0.9 571,221 + MMJJ_RS03035 0.52 +0.3 571,221 + MMJJ_RS03035 0.52 -1.2 571,222 - MMJJ_RS03035 0.52 +0.2 571,223 + MMJJ_RS03035 0.52 -0.8 571,223 + MMJJ_RS03035 0.52 -0.9 571,306 + MMJJ_RS03035 0.67 -0.6 571,310 - MMJJ_RS03035 0.68 +0.0 571,315 + MMJJ_RS03035 0.69 +0.1 571,345 + MMJJ_RS03035 0.74 +0.6 571,345 + MMJJ_RS03035 0.74 -0.6 571,350 + MMJJ_RS03035 0.75 -0.4 571,350 + MMJJ_RS03035 0.75 +0.4 571,471 + -0.0 571,545 - +0.6 571,546 + -0.1 571,546 + -0.2 571,570 + +0.9 571,634 + MMJJ_RS03040 0.20 +1.3 571,719 - MMJJ_RS03040 0.33 +0.2 571,719 - MMJJ_RS03040 0.33 -0.0 571,719 - MMJJ_RS03040 0.33 +0.7 571,719 - MMJJ_RS03040 0.33 +0.2 571,729 + MMJJ_RS03040 0.35 -1.6 571,747 - MMJJ_RS03040 0.37 +0.6 571,747 - MMJJ_RS03040 0.37 +0.8 571,747 - MMJJ_RS03040 0.37 -0.0 571,807 - MMJJ_RS03040 0.47 +0.4 571,807 - MMJJ_RS03040 0.47 -0.7 571,807 - MMJJ_RS03040 0.47 +0.3 571,808 + MMJJ_RS03040 0.47 -0.2 571,827 - MMJJ_RS03040 0.50 -0.6 571,839 - MMJJ_RS03040 0.52 -0.1 571,849 + MMJJ_RS03040 0.53 -0.3 571,852 - MMJJ_RS03040 0.54 +0.7 571,852 - MMJJ_RS03040 0.54 +0.5 571,852 - MMJJ_RS03040 0.54 +0.9 571,852 - MMJJ_RS03040 0.54 +0.2 571,855 + MMJJ_RS03040 0.54 -0.1 571,864 - MMJJ_RS03040 0.56 +0.9 571,865 + MMJJ_RS03040 0.56 +0.3 571,975 - MMJJ_RS03040 0.73 -1.5 572,005 + MMJJ_RS03040 0.78 -0.1 572,005 + MMJJ_RS03040 0.78 +0.9 572,022 + MMJJ_RS03040 0.80 +0.4 572,031 - MMJJ_RS03040 0.82 -0.4 572,032 + MMJJ_RS03040 0.82 -0.4 572,033 - MMJJ_RS03040 0.82 +0.2 572,033 - MMJJ_RS03040 0.82 +0.3 572,033 - MMJJ_RS03040 0.82 +0.6 572,033 - MMJJ_RS03040 0.82 +0.1 572,033 - MMJJ_RS03040 0.82 +0.9 572,034 + MMJJ_RS03040 0.82 -0.0 572,034 + MMJJ_RS03040 0.82 +0.2 572,034 + MMJJ_RS03040 0.82 +0.2 572,034 + MMJJ_RS03040 0.82 +0.9 572,037 - MMJJ_RS03040 0.83 +1.0 572,063 - MMJJ_RS03040 0.87 +0.8 572,205 + +1.0 572,205 + +0.3 572,205 + -0.6 572,205 + -0.2 572,205 + +0.5 572,208 - +0.4 572,213 - -1.1 572,213 - +1.3 572,213 - +0.0 572,213 - +0.4 572,214 + +0.2 572,214 + -0.7 572,214 + +0.6 572,214 + -0.4 572,214 + +0.3 572,214 + +0.2 572,220 + +0.0 572,235 - +0.4 572,245 - +2.7 572,286 - -1.4 572,287 + +0.2 572,336 - +0.1 572,348 - -0.3 572,349 + -0.4 572,355 - +0.7 572,368 + -0.2 572,370 - +0.8 572,371 + +0.1 572,371 + -0.6 572,417 - +0.1 572,443 + +0.3 572,473 - +0.2 572,474 + +0.8 572,474 + -0.4 572,506 + +0.6 572,506 + +0.5 572,506 + +0.7 572,566 - +0.8 572,567 + +0.7 572,582 - MMJJ_RS03045 0.11 +0.8 572,583 + MMJJ_RS03045 0.11 -0.5 572,591 - MMJJ_RS03045 0.15 -1.2 572,592 + MMJJ_RS03045 0.15 -0.3 572,603 - MMJJ_RS03045 0.20 +0.5 572,604 + MMJJ_RS03045 0.21 +1.7 572,627 - MMJJ_RS03045 0.31 +0.3 572,648 - MMJJ_RS03045 0.41 -0.5 572,654 - MMJJ_RS03045 0.44 +0.6 572,655 + MMJJ_RS03045 0.44 -0.2 572,659 - MMJJ_RS03045 0.46 -1.1 572,659 - MMJJ_RS03045 0.46 +0.1 572,683 - MMJJ_RS03045 0.57 +0.2 572,687 - MMJJ_RS03045 0.59 +1.0 572,687 - MMJJ_RS03045 0.59 -0.1 572,687 - MMJJ_RS03045 0.59 +0.8 572,688 + MMJJ_RS03045 0.60 -0.4 572,688 + MMJJ_RS03045 0.60 -0.4 572,688 + MMJJ_RS03045 0.60 -0.6 572,691 - MMJJ_RS03045 0.61 +0.4 572,693 - MMJJ_RS03045 0.62 +0.7 572,694 + MMJJ_RS03045 0.62 +0.5 572,704 + MMJJ_RS03045 0.67 +1.2 572,704 + MMJJ_RS03045 0.67 -0.2 572,704 + MMJJ_RS03045 0.67 +0.6 572,712 - MMJJ_RS03045 0.71 -0.2 572,712 - MMJJ_RS03045 0.71 +0.6 572,712 - MMJJ_RS03045 0.71 +0.8 572,713 + MMJJ_RS03045 0.71 +0.0 572,713 + MMJJ_RS03045 0.71 -0.0 572,713 + MMJJ_RS03045 0.71 +0.4 572,713 + MMJJ_RS03045 0.71 -0.3 572,714 - MMJJ_RS03045 0.72 -0.8 572,717 + MMJJ_RS03045 0.73 -0.0 572,773 + +0.0 572,786 - -0.3 572,790 - -0.5 572,796 - +1.5 572,804 - -0.3 572,805 + +0.8 572,808 - +0.7 572,877 - +0.2 572,877 - -0.3 572,884 - -0.4 572,885 + +0.3 572,885 + -0.7 572,885 + -1.1 572,899 - -0.5 572,942 - -0.0 573,005 - +0.5 573,026 + +2.0 573,028 - +0.1 573,044 - +0.7 573,045 + -0.4 573,045 + +0.2 573,060 - MMJJ_RS03050 0.10 -0.5 573,095 - MMJJ_RS03050 0.13 +0.8 573,100 + MMJJ_RS03050 0.13 +1.2 573,100 + MMJJ_RS03050 0.13 -0.6 573,129 - MMJJ_RS03050 0.15 +1.1 573,139 - MMJJ_RS03050 0.16 +0.6 573,145 - MMJJ_RS03050 0.17 +0.6 573,146 + MMJJ_RS03050 0.17 +0.5
Or see this region's nucleotide sequence