Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01685 and MMJJ_RS01690 are separated by 142 nucleotides MMJJ_RS01690 and MMJJ_RS01695 are separated by 147 nucleotides MMJJ_RS01695 and MMJJ_RS01700 are separated by 39 nucleotides MMJJ_RS01700 and MMJJ_RS01705 are separated by 19 nucleotides
MMJJ_RS01685: MMJJ_RS01685 - DUF354 domain-containing protein, at 326,505 to 327,518
_RS01685
MMJJ_RS01690: MMJJ_RS01690 - methanogenesis marker 12 protein, at 327,661 to 328,638
_RS01690
MMJJ_RS01695: MMJJ_RS01695 - 50S ribosomal protein L7Ae, at 328,786 to 329,139
_RS01695
MMJJ_RS01700: MMJJ_RS01700 - 30S ribosomal protein S28e, at 329,179 to 329,409
_RS01700
MMJJ_RS01705: MMJJ_RS01705 - 50S ribosomal protein L24e, at 329,429 to 329,617
_RS01705
Position (kb) 327
328
329 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 326.778 kb on - strand, within MMJJ_RS01685 at 327.161 kb on - strand, within MMJJ_RS01685 at 327.379 kb on - strand at 327.530 kb on - strand at 327.595 kb on - strand at 327.600 kb on - strand at 327.600 kb on - strand at 327.615 kb on - strand at 327.615 kb on - strand at 327.615 kb on - strand at 327.616 kb on + strand at 327.617 kb on - strand at 327.617 kb on - strand at 327.617 kb on - strand at 327.617 kb on - strand at 327.618 kb on + strand at 327.618 kb on + strand at 327.627 kb on - strand at 327.628 kb on + strand at 327.638 kb on - strand at 327.641 kb on - strand at 327.642 kb on + strand at 327.660 kb on - strand at 327.970 kb on + strand, within MMJJ_RS01690 at 328.088 kb on + strand, within MMJJ_RS01690 at 328.088 kb on + strand, within MMJJ_RS01690 at 328.209 kb on + strand, within MMJJ_RS01690 at 328.239 kb on - strand, within MMJJ_RS01690 at 328.240 kb on + strand, within MMJJ_RS01690 at 328.246 kb on + strand, within MMJJ_RS01690 at 328.316 kb on + strand, within MMJJ_RS01690 at 328.354 kb on + strand, within MMJJ_RS01690 at 328.566 kb on + strand at 328.595 kb on - strand at 328.661 kb on - strand at 328.661 kb on - strand at 328.675 kb on - strand at 328.796 kb on + strand at 329.602 kb on - strand at 329.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 326,778 - MMJJ_RS01685 0.27 +0.9 327,161 - MMJJ_RS01685 0.65 -0.7 327,379 - -1.4 327,530 - -0.6 327,595 - -2.1 327,600 - +0.5 327,600 - +0.2 327,615 - +0.7 327,615 - +1.7 327,615 - -0.3 327,616 + -1.1 327,617 - -0.3 327,617 - +0.6 327,617 - +0.6 327,617 - -0.0 327,618 + +1.3 327,618 + +0.5 327,627 - -0.1 327,628 + +0.4 327,638 - -0.2 327,641 - -0.2 327,642 + +0.3 327,660 - -3.3 327,970 + MMJJ_RS01690 0.32 -1.3 328,088 + MMJJ_RS01690 0.44 -3.2 328,088 + MMJJ_RS01690 0.44 +0.0 328,209 + MMJJ_RS01690 0.56 -3.0 328,239 - MMJJ_RS01690 0.59 -2.3 328,240 + MMJJ_RS01690 0.59 +0.2 328,246 + MMJJ_RS01690 0.60 -1.4 328,316 + MMJJ_RS01690 0.67 +0.6 328,354 + MMJJ_RS01690 0.71 -1.9 328,566 + -1.0 328,595 - -4.1 328,661 - +0.2 328,661 - -0.1 328,675 - -0.7 328,796 + -1.7 329,602 - +0.5 329,615 - -0.1
Or see this region's nucleotide sequence