Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00925 and MMJJ_RS00930 are separated by 86 nucleotides MMJJ_RS00930 and MMJJ_RS00935 are separated by 46 nucleotides MMJJ_RS00935 and MMJJ_RS00940 are separated by 11 nucleotides
MMJJ_RS00925: MMJJ_RS00925 - hypothetical protein, at 179,848 to 180,360
_RS00925
MMJJ_RS00930: MMJJ_RS00930 - queuosine precursor transporter, at 180,447 to 181,055
_RS00930
MMJJ_RS00935: MMJJ_RS00935 - nickel pincer cofactor biosynthesis protein LarB, at 181,102 to 181,887
_RS00935
MMJJ_RS00940: MMJJ_RS00940 - aldo/keto reductase, at 181,899 to 183,092
_RS00940
Position (kb) 181
182 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 180.110 kb on + strand, within MMJJ_RS00925 at 180.151 kb on + strand, within MMJJ_RS00925 at 180.177 kb on - strand, within MMJJ_RS00925 at 180.178 kb on + strand, within MMJJ_RS00925 at 180.247 kb on + strand, within MMJJ_RS00925 at 180.273 kb on - strand, within MMJJ_RS00925 at 180.358 kb on - strand at 180.385 kb on - strand at 180.388 kb on - strand at 180.389 kb on + strand at 180.411 kb on - strand at 180.412 kb on + strand at 180.423 kb on - strand at 180.424 kb on + strand at 180.432 kb on - strand at 180.432 kb on - strand at 180.432 kb on - strand at 180.433 kb on + strand at 180.433 kb on + strand at 180.436 kb on - strand at 180.436 kb on - strand at 180.436 kb on - strand at 180.437 kb on + strand at 180.437 kb on + strand at 180.437 kb on + strand at 180.489 kb on - strand at 180.517 kb on - strand, within MMJJ_RS00930 at 180.518 kb on + strand, within MMJJ_RS00930 at 180.585 kb on - strand, within MMJJ_RS00930 at 180.599 kb on - strand, within MMJJ_RS00930 at 180.601 kb on - strand, within MMJJ_RS00930 at 180.604 kb on - strand, within MMJJ_RS00930 at 180.604 kb on - strand, within MMJJ_RS00930 at 180.605 kb on + strand, within MMJJ_RS00930 at 180.605 kb on + strand, within MMJJ_RS00930 at 180.605 kb on + strand, within MMJJ_RS00930 at 180.840 kb on - strand, within MMJJ_RS00930 at 180.859 kb on - strand, within MMJJ_RS00930 at 180.867 kb on + strand, within MMJJ_RS00930 at 180.882 kb on + strand, within MMJJ_RS00930 at 180.890 kb on + strand, within MMJJ_RS00930 at 180.914 kb on + strand, within MMJJ_RS00930 at 180.995 kb on - strand at 181.001 kb on + strand at 181.022 kb on - strand at 181.023 kb on + strand at 181.024 kb on - strand at 181.024 kb on - strand at 181.025 kb on + strand at 181.026 kb on - strand at 181.027 kb on + strand at 181.091 kb on + strand at 181.091 kb on + strand at 181.091 kb on + strand at 181.116 kb on - strand at 181.116 kb on - strand at 181.117 kb on + strand at 181.193 kb on + strand, within MMJJ_RS00935 at 181.202 kb on + strand, within MMJJ_RS00935 at 181.378 kb on - strand, within MMJJ_RS00935 at 181.382 kb on + strand, within MMJJ_RS00935 at 181.430 kb on - strand, within MMJJ_RS00935 at 181.430 kb on - strand, within MMJJ_RS00935 at 181.430 kb on - strand, within MMJJ_RS00935 at 181.430 kb on - strand, within MMJJ_RS00935 at 181.431 kb on + strand, within MMJJ_RS00935 at 181.431 kb on + strand, within MMJJ_RS00935 at 181.431 kb on + strand, within MMJJ_RS00935 at 181.432 kb on - strand, within MMJJ_RS00935 at 181.433 kb on + strand, within MMJJ_RS00935 at 181.433 kb on + strand, within MMJJ_RS00935 at 181.486 kb on - strand, within MMJJ_RS00935 at 181.487 kb on + strand, within MMJJ_RS00935 at 181.495 kb on + strand, within MMJJ_RS00935 at 181.504 kb on + strand, within MMJJ_RS00935 at 181.513 kb on + strand, within MMJJ_RS00935 at 181.516 kb on - strand, within MMJJ_RS00935 at 181.516 kb on - strand, within MMJJ_RS00935 at 181.517 kb on + strand, within MMJJ_RS00935 at 181.525 kb on - strand, within MMJJ_RS00935 at 181.526 kb on + strand, within MMJJ_RS00935 at 181.558 kb on + strand, within MMJJ_RS00935 at 181.609 kb on + strand, within MMJJ_RS00935 at 181.633 kb on + strand, within MMJJ_RS00935 at 181.647 kb on - strand, within MMJJ_RS00935 at 181.647 kb on - strand, within MMJJ_RS00935 at 181.647 kb on - strand, within MMJJ_RS00935 at 181.657 kb on - strand, within MMJJ_RS00935 at 181.780 kb on + strand, within MMJJ_RS00935 at 181.798 kb on - strand, within MMJJ_RS00935 at 181.872 kb on - strand at 181.901 kb on + strand at 181.901 kb on + strand at 181.965 kb on - strand at 181.997 kb on - strand at 182.016 kb on - strand at 182.016 kb on - strand at 182.017 kb on + strand at 182.017 kb on + strand at 182.018 kb on - strand at 182.073 kb on - strand, within MMJJ_RS00940 at 182.073 kb on - strand, within MMJJ_RS00940 at 182.074 kb on + strand, within MMJJ_RS00940 at 182.074 kb on + strand, within MMJJ_RS00940 at 182.074 kb on + strand, within MMJJ_RS00940 at 182.074 kb on + strand, within MMJJ_RS00940 at 182.074 kb on + strand, within MMJJ_RS00940 at 182.075 kb on - strand, within MMJJ_RS00940 at 182.081 kb on - strand, within MMJJ_RS00940 at 182.081 kb on - strand, within MMJJ_RS00940 at 182.082 kb on + strand, within MMJJ_RS00940 at 182.131 kb on + strand, within MMJJ_RS00940 at 182.141 kb on + strand, within MMJJ_RS00940 at 182.141 kb on + strand, within MMJJ_RS00940 at 182.141 kb on + strand, within MMJJ_RS00940 at 182.187 kb on + strand, within MMJJ_RS00940 at 182.205 kb on + strand, within MMJJ_RS00940 at 182.211 kb on + strand, within MMJJ_RS00940 at 182.211 kb on + strand, within MMJJ_RS00940 at 182.231 kb on + strand, within MMJJ_RS00940 at 182.250 kb on - strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.308 kb on + strand, within MMJJ_RS00940 at 182.308 kb on + strand, within MMJJ_RS00940 at 182.397 kb on - strand, within MMJJ_RS00940 at 182.415 kb on + strand, within MMJJ_RS00940 at 182.521 kb on - strand, within MMJJ_RS00940 at 182.522 kb on + strand, within MMJJ_RS00940 at 182.560 kb on + strand, within MMJJ_RS00940 at 182.563 kb on - strand, within MMJJ_RS00940 at 182.568 kb on + strand, within MMJJ_RS00940 at 182.570 kb on + strand, within MMJJ_RS00940 at 182.574 kb on - strand, within MMJJ_RS00940 at 182.574 kb on - strand, within MMJJ_RS00940 at 182.574 kb on - strand, within MMJJ_RS00940 at 182.574 kb on - strand, within MMJJ_RS00940 at 182.574 kb on - strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.596 kb on - strand, within MMJJ_RS00940 at 182.664 kb on + strand, within MMJJ_RS00940 at 182.664 kb on + strand, within MMJJ_RS00940 at 182.698 kb on + strand, within MMJJ_RS00940 at 182.736 kb on + strand, within MMJJ_RS00940 at 182.749 kb on + strand, within MMJJ_RS00940 at 182.752 kb on - strand, within MMJJ_RS00940 at 182.762 kb on - strand, within MMJJ_RS00940 at 182.768 kb on - strand, within MMJJ_RS00940 at 182.768 kb on - strand, within MMJJ_RS00940 at 182.769 kb on + strand, within MMJJ_RS00940 at 182.805 kb on + strand, within MMJJ_RS00940 at 182.810 kb on - strand, within MMJJ_RS00940 at 182.826 kb on - strand, within MMJJ_RS00940 at 182.859 kb on + strand, within MMJJ_RS00940
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 180,110 + MMJJ_RS00925 0.51 +0.2 180,151 + MMJJ_RS00925 0.59 +0.1 180,177 - MMJJ_RS00925 0.64 +0.0 180,178 + MMJJ_RS00925 0.64 -2.4 180,247 + MMJJ_RS00925 0.78 +0.1 180,273 - MMJJ_RS00925 0.83 -0.9 180,358 - -0.3 180,385 - -0.5 180,388 - +0.8 180,389 + +0.1 180,411 - -1.9 180,412 + +0.2 180,423 - +0.2 180,424 + +0.7 180,432 - +0.2 180,432 - -0.2 180,432 - -0.7 180,433 + +0.9 180,433 + -1.9 180,436 - -1.1 180,436 - +0.5 180,436 - -0.5 180,437 + -0.3 180,437 + -0.3 180,437 + -0.0 180,489 - +0.4 180,517 - MMJJ_RS00930 0.11 -1.5 180,518 + MMJJ_RS00930 0.12 -0.5 180,585 - MMJJ_RS00930 0.23 +0.5 180,599 - MMJJ_RS00930 0.25 -0.7 180,601 - MMJJ_RS00930 0.25 +0.2 180,604 - MMJJ_RS00930 0.26 -0.1 180,604 - MMJJ_RS00930 0.26 -0.8 180,605 + MMJJ_RS00930 0.26 -1.3 180,605 + MMJJ_RS00930 0.26 -0.0 180,605 + MMJJ_RS00930 0.26 -0.3 180,840 - MMJJ_RS00930 0.65 -1.1 180,859 - MMJJ_RS00930 0.68 +0.5 180,867 + MMJJ_RS00930 0.69 -0.0 180,882 + MMJJ_RS00930 0.71 -0.5 180,890 + MMJJ_RS00930 0.73 +0.0 180,914 + MMJJ_RS00930 0.77 -0.3 180,995 - +0.6 181,001 + +0.7 181,022 - -0.3 181,023 + +0.2 181,024 - -0.5 181,024 - -0.9 181,025 + -0.7 181,026 - -1.9 181,027 + -0.7 181,091 + +0.2 181,091 + +0.2 181,091 + -0.1 181,116 - -1.1 181,116 - +0.7 181,117 + -0.9 181,193 + MMJJ_RS00935 0.12 -1.7 181,202 + MMJJ_RS00935 0.13 -3.3 181,378 - MMJJ_RS00935 0.35 -2.3 181,382 + MMJJ_RS00935 0.36 -2.2 181,430 - MMJJ_RS00935 0.42 -1.3 181,430 - MMJJ_RS00935 0.42 -2.0 181,430 - MMJJ_RS00935 0.42 -0.5 181,430 - MMJJ_RS00935 0.42 -2.0 181,431 + MMJJ_RS00935 0.42 -1.7 181,431 + MMJJ_RS00935 0.42 -2.4 181,431 + MMJJ_RS00935 0.42 -2.2 181,432 - MMJJ_RS00935 0.42 -2.2 181,433 + MMJJ_RS00935 0.42 -2.8 181,433 + MMJJ_RS00935 0.42 -1.9 181,486 - MMJJ_RS00935 0.49 -1.3 181,487 + MMJJ_RS00935 0.49 -1.1 181,495 + MMJJ_RS00935 0.50 -0.4 181,504 + MMJJ_RS00935 0.51 -2.0 181,513 + MMJJ_RS00935 0.52 -2.0 181,516 - MMJJ_RS00935 0.53 -2.3 181,516 - MMJJ_RS00935 0.53 -2.9 181,517 + MMJJ_RS00935 0.53 -3.0 181,525 - MMJJ_RS00935 0.54 -2.3 181,526 + MMJJ_RS00935 0.54 -1.9 181,558 + MMJJ_RS00935 0.58 -2.7 181,609 + MMJJ_RS00935 0.65 -2.3 181,633 + MMJJ_RS00935 0.68 -2.2 181,647 - MMJJ_RS00935 0.69 -2.3 181,647 - MMJJ_RS00935 0.69 -1.8 181,647 - MMJJ_RS00935 0.69 -2.4 181,657 - MMJJ_RS00935 0.71 -2.3 181,780 + MMJJ_RS00935 0.86 -2.0 181,798 - MMJJ_RS00935 0.89 -3.8 181,872 - -4.2 181,901 + +0.9 181,901 + -0.8 181,965 - +0.0 181,997 - +0.4 182,016 - -0.3 182,016 - -0.5 182,017 + -0.7 182,017 + +0.5 182,018 - -1.5 182,073 - MMJJ_RS00940 0.15 +0.2 182,073 - MMJJ_RS00940 0.15 -0.2 182,074 + MMJJ_RS00940 0.15 -1.3 182,074 + MMJJ_RS00940 0.15 -0.1 182,074 + MMJJ_RS00940 0.15 +0.8 182,074 + MMJJ_RS00940 0.15 -0.1 182,074 + MMJJ_RS00940 0.15 +2.9 182,075 - MMJJ_RS00940 0.15 -0.7 182,081 - MMJJ_RS00940 0.15 -0.4 182,081 - MMJJ_RS00940 0.15 +0.1 182,082 + MMJJ_RS00940 0.15 -0.0 182,131 + MMJJ_RS00940 0.19 +0.4 182,141 + MMJJ_RS00940 0.20 +0.9 182,141 + MMJJ_RS00940 0.20 -0.1 182,141 + MMJJ_RS00940 0.20 +0.9 182,187 + MMJJ_RS00940 0.24 -0.2 182,205 + MMJJ_RS00940 0.26 -0.2 182,211 + MMJJ_RS00940 0.26 +0.4 182,211 + MMJJ_RS00940 0.26 +1.0 182,231 + MMJJ_RS00940 0.28 +0.2 182,250 - MMJJ_RS00940 0.29 -1.5 182,251 + MMJJ_RS00940 0.29 -0.5 182,251 + MMJJ_RS00940 0.29 -0.3 182,251 + MMJJ_RS00940 0.29 +0.6 182,251 + MMJJ_RS00940 0.29 +0.7 182,251 + MMJJ_RS00940 0.29 -1.7 182,251 + MMJJ_RS00940 0.29 -0.0 182,308 + MMJJ_RS00940 0.34 -0.0 182,308 + MMJJ_RS00940 0.34 +0.4 182,397 - MMJJ_RS00940 0.42 -0.3 182,415 + MMJJ_RS00940 0.43 +0.9 182,521 - MMJJ_RS00940 0.52 +0.9 182,522 + MMJJ_RS00940 0.52 +0.9 182,560 + MMJJ_RS00940 0.55 -0.0 182,563 - MMJJ_RS00940 0.56 +0.9 182,568 + MMJJ_RS00940 0.56 +1.2 182,570 + MMJJ_RS00940 0.56 -0.2 182,574 - MMJJ_RS00940 0.57 +0.2 182,574 - MMJJ_RS00940 0.57 -0.9 182,574 - MMJJ_RS00940 0.57 -0.1 182,574 - MMJJ_RS00940 0.57 -1.1 182,574 - MMJJ_RS00940 0.57 -0.4 182,575 + MMJJ_RS00940 0.57 +0.7 182,575 + MMJJ_RS00940 0.57 -0.9 182,575 + MMJJ_RS00940 0.57 +1.1 182,575 + MMJJ_RS00940 0.57 -0.0 182,575 + MMJJ_RS00940 0.57 +0.1 182,575 + MMJJ_RS00940 0.57 -0.3 182,575 + MMJJ_RS00940 0.57 -0.4 182,596 - MMJJ_RS00940 0.58 +0.3 182,664 + MMJJ_RS00940 0.64 +0.2 182,664 + MMJJ_RS00940 0.64 -0.1 182,698 + MMJJ_RS00940 0.67 +0.4 182,736 + MMJJ_RS00940 0.70 +0.2 182,749 + MMJJ_RS00940 0.71 -1.1 182,752 - MMJJ_RS00940 0.71 +0.3 182,762 - MMJJ_RS00940 0.72 -0.2 182,768 - MMJJ_RS00940 0.73 +0.1 182,768 - MMJJ_RS00940 0.73 +0.5 182,769 + MMJJ_RS00940 0.73 -0.4 182,805 + MMJJ_RS00940 0.76 +0.5 182,810 - MMJJ_RS00940 0.76 -0.6 182,826 - MMJJ_RS00940 0.78 -1.1 182,859 + MMJJ_RS00940 0.80 -0.1
Or see this region's nucleotide sequence