Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00360 and MMJJ_RS00365 are separated by 31 nucleotides MMJJ_RS00365 and MMJJ_RS00370 are separated by 96 nucleotides MMJJ_RS00370 and MMJJ_RS00375 are separated by 30 nucleotides MMJJ_RS00375 and MMJJ_RS00380 are separated by 63 nucleotides
MMJJ_RS00360: MMJJ_RS00360 - adenosylhomocysteinase, at 68,397 to 69,644
_RS00360
MMJJ_RS00365: MMJJ_RS00365 - dihydroorotate dehydrogenase electron transfer subunit, at 69,676 to 70,443
_RS00365
MMJJ_RS00370: MMJJ_RS00370 - asparagine synthase (glutamine-hydrolyzing), at 70,540 to 72,081
_RS00370
MMJJ_RS00375: MMJJ_RS00375 - diaminopimelate epimerase, at 72,112 to 72,945
_RS00375
MMJJ_RS00380: MMJJ_RS00380 - PRC-barrel domain-containing protein, at 73,009 to 73,257
_RS00380
Position (kb)
70
71
72
73 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 70.074 kb on + strand at 70.149 kb on - strand, within MMJJ_RS00365 at 70.150 kb on + strand, within MMJJ_RS00365 at 70.257 kb on - strand, within MMJJ_RS00365 at 70.395 kb on + strand at 70.411 kb on - strand at 70.445 kb on + strand at 70.463 kb on - strand at 70.463 kb on - strand at 70.467 kb on - strand at 70.468 kb on + strand at 70.472 kb on + strand at 70.476 kb on - strand at 70.481 kb on + strand at 70.481 kb on + strand at 70.483 kb on + strand at 70.483 kb on + strand at 70.625 kb on - strand at 71.103 kb on + strand, within MMJJ_RS00370 at 71.528 kb on + strand, within MMJJ_RS00370 at 71.576 kb on - strand, within MMJJ_RS00370 at 71.790 kb on - strand, within MMJJ_RS00370 at 71.996 kb on + strand at 72.020 kb on + strand at 72.056 kb on + strand at 72.056 kb on + strand at 72.060 kb on + strand at 72.060 kb on + strand at 72.060 kb on + strand at 72.090 kb on - strand at 72.096 kb on + strand at 72.103 kb on + strand at 72.113 kb on - strand at 72.162 kb on + strand at 72.898 kb on - strand at 72.908 kb on - strand at 72.909 kb on + strand at 72.968 kb on - strand at 72.993 kb on - strand at 73.010 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 70,074 + -0.4 70,149 - MMJJ_RS00365 0.62 -1.9 70,150 + MMJJ_RS00365 0.62 -0.9 70,257 - MMJJ_RS00365 0.76 -0.8 70,395 + -0.9 70,411 - -1.8 70,445 + -0.1 70,463 - -0.6 70,463 - +0.3 70,467 - -0.4 70,468 + +0.9 70,472 + +0.0 70,476 - -0.2 70,481 + -2.2 70,481 + +0.3 70,483 + -0.3 70,483 + +0.0 70,625 - -0.1 71,103 + MMJJ_RS00370 0.37 -1.1 71,528 + MMJJ_RS00370 0.64 -0.7 71,576 - MMJJ_RS00370 0.67 -2.1 71,790 - MMJJ_RS00370 0.81 -0.7 71,996 + -3.0 72,020 + -2.3 72,056 + -0.5 72,056 + -1.5 72,060 + -0.3 72,060 + -1.0 72,060 + -0.7 72,090 - +0.1 72,096 + -0.4 72,103 + +0.6 72,113 - -0.1 72,162 + -2.9 72,898 - -2.1 72,908 - -1.6 72,909 + -2.3 72,968 - -2.1 72,993 - -0.5 73,010 - -0.3
Or see this region's nucleotide sequence