Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00255 and MMJJ_RS00260 are separated by 108 nucleotides MMJJ_RS00260 and MMJJ_RS00265 are separated by 22 nucleotides MMJJ_RS00265 and MMJJ_RS00270 are separated by 15 nucleotides MMJJ_RS00270 and MMJJ_RS00275 are separated by 53 nucleotides
MMJJ_RS00255: MMJJ_RS00255 - phosphoadenosine phosphosulfate reductase family protein, at 49,881 to 51,392
_RS00255
MMJJ_RS00260: MMJJ_RS00260 - energy-converting hydrogenase B subunit EhbP, at 51,501 to 51,788
_RS00260
MMJJ_RS00265: MMJJ_RS00265 - TIGR02253 family HAD-type hydrolase, at 51,811 to 52,488
_RS00265
MMJJ_RS00270: MMJJ_RS00270 - adenosylcobinamide-GDP ribazoletransferase, at 52,504 to 53,277
_RS00270
MMJJ_RS00275: MMJJ_RS00275 - coenzyme F420-0:L-glutamate ligase, at 53,331 to 54,077
_RS00275
Position (kb)
51
52
53 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 50.823 kb on + strand, within MMJJ_RS00255 at 50.823 kb on + strand, within MMJJ_RS00255 at 50.826 kb on - strand, within MMJJ_RS00255 at 50.826 kb on - strand, within MMJJ_RS00255 at 50.826 kb on - strand, within MMJJ_RS00255 at 50.827 kb on + strand, within MMJJ_RS00255 at 50.827 kb on + strand, within MMJJ_RS00255 at 50.827 kb on + strand, within MMJJ_RS00255 at 50.924 kb on + strand, within MMJJ_RS00255 at 50.927 kb on - strand, within MMJJ_RS00255 at 50.927 kb on - strand, within MMJJ_RS00255 at 50.980 kb on - strand, within MMJJ_RS00255 at 50.996 kb on - strand, within MMJJ_RS00255 at 51.044 kb on - strand, within MMJJ_RS00255 at 51.184 kb on - strand, within MMJJ_RS00255 at 51.185 kb on + strand, within MMJJ_RS00255 at 51.185 kb on + strand, within MMJJ_RS00255 at 51.217 kb on - strand, within MMJJ_RS00255 at 51.409 kb on - strand at 51.409 kb on - strand at 51.409 kb on - strand at 51.409 kb on - strand at 51.410 kb on + strand at 51.410 kb on + strand at 51.410 kb on + strand at 51.410 kb on + strand at 51.410 kb on + strand at 51.410 kb on + strand at 51.411 kb on - strand at 51.411 kb on - strand at 51.412 kb on + strand at 51.412 kb on + strand at 51.412 kb on + strand at 51.412 kb on + strand at 51.412 kb on + strand at 51.412 kb on + strand at 51.415 kb on + strand at 51.415 kb on + strand at 51.426 kb on + strand at 51.427 kb on - strand at 51.427 kb on - strand at 51.427 kb on - strand at 51.427 kb on - strand at 51.428 kb on + strand at 51.436 kb on - strand at 51.436 kb on - strand at 51.436 kb on - strand at 51.437 kb on + strand at 51.453 kb on - strand at 51.455 kb on - strand at 51.455 kb on - strand at 51.455 kb on - strand at 51.456 kb on + strand at 51.470 kb on + strand at 51.472 kb on + strand at 51.474 kb on - strand at 51.576 kb on + strand, within MMJJ_RS00260 at 51.589 kb on - strand, within MMJJ_RS00260 at 51.791 kb on - strand at 51.791 kb on - strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.817 kb on + strand at 51.894 kb on + strand, within MMJJ_RS00265 at 51.925 kb on + strand, within MMJJ_RS00265 at 51.947 kb on - strand, within MMJJ_RS00265 at 52.027 kb on - strand, within MMJJ_RS00265 at 52.059 kb on - strand, within MMJJ_RS00265 at 52.105 kb on + strand, within MMJJ_RS00265 at 52.113 kb on - strand, within MMJJ_RS00265 at 52.118 kb on - strand, within MMJJ_RS00265 at 52.171 kb on + strand, within MMJJ_RS00265 at 52.230 kb on - strand, within MMJJ_RS00265 at 52.231 kb on + strand, within MMJJ_RS00265 at 52.231 kb on + strand, within MMJJ_RS00265 at 52.233 kb on + strand, within MMJJ_RS00265 at 52.283 kb on + strand, within MMJJ_RS00265 at 52.305 kb on + strand, within MMJJ_RS00265 at 52.427 kb on - strand at 52.428 kb on + strand at 52.452 kb on + strand at 52.482 kb on - strand at 52.654 kb on + strand, within MMJJ_RS00270 at 52.655 kb on - strand, within MMJJ_RS00270 at 52.719 kb on + strand, within MMJJ_RS00270 at 52.723 kb on + strand, within MMJJ_RS00270 at 52.810 kb on + strand, within MMJJ_RS00270 at 52.810 kb on + strand, within MMJJ_RS00270 at 52.928 kb on + strand, within MMJJ_RS00270 at 52.934 kb on - strand, within MMJJ_RS00270 at 53.082 kb on + strand at 53.257 kb on - strand at 53.269 kb on + strand at 53.279 kb on - strand at 53.300 kb on + strand at 53.305 kb on - strand at 53.309 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 50,823 + MMJJ_RS00255 0.62 +0.1 50,823 + MMJJ_RS00255 0.62 +0.2 50,826 - MMJJ_RS00255 0.62 +0.4 50,826 - MMJJ_RS00255 0.62 +0.4 50,826 - MMJJ_RS00255 0.62 -0.2 50,827 + MMJJ_RS00255 0.63 +0.3 50,827 + MMJJ_RS00255 0.63 +0.4 50,827 + MMJJ_RS00255 0.63 -1.2 50,924 + MMJJ_RS00255 0.69 +1.5 50,927 - MMJJ_RS00255 0.69 +0.1 50,927 - MMJJ_RS00255 0.69 +0.5 50,980 - MMJJ_RS00255 0.73 -1.4 50,996 - MMJJ_RS00255 0.74 -0.1 51,044 - MMJJ_RS00255 0.77 +0.7 51,184 - MMJJ_RS00255 0.86 -0.9 51,185 + MMJJ_RS00255 0.86 -0.5 51,185 + MMJJ_RS00255 0.86 -2.3 51,217 - MMJJ_RS00255 0.88 -2.5 51,409 - -0.4 51,409 - -0.8 51,409 - -0.6 51,409 - -0.2 51,410 + +0.4 51,410 + +0.2 51,410 + -0.6 51,410 + -0.7 51,410 + +0.3 51,410 + -0.7 51,411 - +0.7 51,411 - -0.3 51,412 + -0.7 51,412 + -0.3 51,412 + -1.4 51,412 + +0.8 51,412 + -0.8 51,412 + -0.5 51,415 + -0.4 51,415 + +0.1 51,426 + +1.3 51,427 - -1.3 51,427 - +0.5 51,427 - +1.7 51,427 - -0.4 51,428 + -0.4 51,436 - -0.8 51,436 - -0.5 51,436 - -0.1 51,437 + -0.5 51,453 - -0.7 51,455 - -0.4 51,455 - -1.8 51,455 - -1.1 51,456 + -0.8 51,470 + -1.6 51,472 + -1.3 51,474 - -1.4 51,576 + MMJJ_RS00260 0.26 -2.8 51,589 - MMJJ_RS00260 0.31 -0.4 51,791 - -0.3 51,791 - -1.0 51,794 + -0.8 51,794 + -0.5 51,794 + -1.2 51,794 + -1.5 51,817 + -1.2 51,894 + MMJJ_RS00265 0.12 -1.8 51,925 + MMJJ_RS00265 0.17 -2.4 51,947 - MMJJ_RS00265 0.20 -1.5 52,027 - MMJJ_RS00265 0.32 -2.4 52,059 - MMJJ_RS00265 0.37 -0.4 52,105 + MMJJ_RS00265 0.43 -1.7 52,113 - MMJJ_RS00265 0.45 -1.0 52,118 - MMJJ_RS00265 0.45 -0.7 52,171 + MMJJ_RS00265 0.53 -1.2 52,230 - MMJJ_RS00265 0.62 -2.0 52,231 + MMJJ_RS00265 0.62 -2.5 52,231 + MMJJ_RS00265 0.62 -1.6 52,233 + MMJJ_RS00265 0.62 -1.7 52,283 + MMJJ_RS00265 0.70 -2.3 52,305 + MMJJ_RS00265 0.73 -0.3 52,427 - -0.3 52,428 + -0.3 52,452 + -1.9 52,482 - +0.0 52,654 + MMJJ_RS00270 0.19 -1.4 52,655 - MMJJ_RS00270 0.20 -1.0 52,719 + MMJJ_RS00270 0.28 -1.8 52,723 + MMJJ_RS00270 0.28 -1.0 52,810 + MMJJ_RS00270 0.40 -1.1 52,810 + MMJJ_RS00270 0.40 -3.3 52,928 + MMJJ_RS00270 0.55 -1.9 52,934 - MMJJ_RS00270 0.56 -1.9 53,082 + -0.7 53,257 - +0.6 53,269 + -1.1 53,279 - +0.9 53,300 + +1.2 53,305 - -0.5 53,309 - +0.0
Or see this region's nucleotide sequence