Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00195 and MMJJ_RS00200 are separated by 142 nucleotides MMJJ_RS00200 and MMJJ_RS00205 are separated by 92 nucleotides MMJJ_RS00205 and MMJJ_RS00210 are separated by 10 nucleotides
MMJJ_RS00195: MMJJ_RS00195 - precorrin-3B C(17)-methyltransferase, at 37,263 to 38,015
_RS00195
MMJJ_RS00200: MMJJ_RS00200 - translation initiation factor IF-5A, at 38,158 to 38,553
_RS00200
MMJJ_RS00205: MMJJ_RS00205 - adenosylcobinamide-phosphate synthase CbiB, at 38,646 to 39,566
_RS00205
MMJJ_RS00210: MMJJ_RS00210 - DUF530 family protein, at 39,577 to 41,007
_RS00210
Position (kb) 38
39
40 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 37.809 kb on + strand at 37.908 kb on + strand, within MMJJ_RS00195 at 37.995 kb on - strand at 37.996 kb on + strand at 37.996 kb on + strand at 37.997 kb on - strand at 37.997 kb on - strand at 37.997 kb on - strand at 37.997 kb on - strand at 37.997 kb on - strand at 37.998 kb on + strand at 37.998 kb on + strand at 37.998 kb on + strand at 38.008 kb on + strand at 38.024 kb on - strand at 38.040 kb on - strand at 38.045 kb on + strand at 38.045 kb on + strand at 38.045 kb on + strand at 38.045 kb on + strand at 38.045 kb on + strand at 38.046 kb on - strand at 38.046 kb on - strand at 38.046 kb on - strand at 38.047 kb on + strand at 38.047 kb on + strand at 38.047 kb on + strand at 38.047 kb on + strand at 38.047 kb on + strand at 38.047 kb on + strand at 38.058 kb on + strand at 38.090 kb on - strand at 38.090 kb on - strand at 38.123 kb on + strand at 38.136 kb on + strand at 38.304 kb on - strand, within MMJJ_RS00200 at 38.334 kb on + strand, within MMJJ_RS00200 at 38.551 kb on - strand at 38.580 kb on - strand at 38.665 kb on - strand at 38.665 kb on - strand at 38.755 kb on - strand, within MMJJ_RS00205 at 38.758 kb on - strand, within MMJJ_RS00205 at 38.780 kb on - strand, within MMJJ_RS00205 at 38.784 kb on - strand, within MMJJ_RS00205 at 38.903 kb on - strand, within MMJJ_RS00205 at 38.983 kb on + strand, within MMJJ_RS00205 at 39.090 kb on + strand, within MMJJ_RS00205 at 39.153 kb on - strand, within MMJJ_RS00205 at 39.165 kb on + strand, within MMJJ_RS00205 at 39.175 kb on + strand, within MMJJ_RS00205 at 39.175 kb on + strand, within MMJJ_RS00205 at 39.175 kb on + strand, within MMJJ_RS00205 at 39.180 kb on + strand, within MMJJ_RS00205 at 39.180 kb on + strand, within MMJJ_RS00205 at 39.309 kb on + strand, within MMJJ_RS00205 at 39.777 kb on + strand, within MMJJ_RS00210 at 40.416 kb on - strand at 40.450 kb on - strand at 40.490 kb on + strand, within MMJJ_RS00210
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 37,809 + -1.1 37,908 + MMJJ_RS00195 0.86 -1.0 37,995 - -1.4 37,996 + -1.1 37,996 + +0.3 37,997 - +0.8 37,997 - +0.8 37,997 - -0.2 37,997 - +0.1 37,997 - +0.6 37,998 + +1.2 37,998 + +0.4 37,998 + -1.5 38,008 + -0.6 38,024 - +0.5 38,040 - -0.5 38,045 + +0.9 38,045 + -1.1 38,045 + -1.0 38,045 + -0.2 38,045 + -0.8 38,046 - -0.2 38,046 - +1.1 38,046 - -0.7 38,047 + -0.4 38,047 + +0.3 38,047 + -1.7 38,047 + -0.3 38,047 + +0.5 38,047 + +0.5 38,058 + +0.9 38,090 - -1.0 38,090 - +0.1 38,123 + -1.1 38,136 + -2.8 38,304 - MMJJ_RS00200 0.37 -0.7 38,334 + MMJJ_RS00200 0.44 +1.4 38,551 - +0.5 38,580 - +0.7 38,665 - -1.9 38,665 - +0.8 38,755 - MMJJ_RS00205 0.12 -3.7 38,758 - MMJJ_RS00205 0.12 -3.6 38,780 - MMJJ_RS00205 0.15 -1.5 38,784 - MMJJ_RS00205 0.15 -1.4 38,903 - MMJJ_RS00205 0.28 +0.2 38,983 + MMJJ_RS00205 0.37 -1.0 39,090 + MMJJ_RS00205 0.48 -0.4 39,153 - MMJJ_RS00205 0.55 -0.1 39,165 + MMJJ_RS00205 0.56 -1.9 39,175 + MMJJ_RS00205 0.57 -0.7 39,175 + MMJJ_RS00205 0.57 -0.3 39,175 + MMJJ_RS00205 0.57 -1.4 39,180 + MMJJ_RS00205 0.58 -1.1 39,180 + MMJJ_RS00205 0.58 -2.1 39,309 + MMJJ_RS00205 0.72 -2.3 39,777 + MMJJ_RS00210 0.14 -1.7 40,416 - -0.7 40,450 - -1.1 40,490 + MMJJ_RS00210 0.64 -2.7
Or see this region's nucleotide sequence