Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00190 and MMJJ_RS00195 are separated by 288 nucleotides MMJJ_RS00195 and MMJJ_RS00200 are separated by 142 nucleotides MMJJ_RS00200 and MMJJ_RS00205 are separated by 92 nucleotides
MMJJ_RS00190: MMJJ_RS00190 - hypothetical protein, at 36,501 to 36,974
_RS00190
MMJJ_RS00195: MMJJ_RS00195 - precorrin-3B C(17)-methyltransferase, at 37,263 to 38,015
_RS00195
MMJJ_RS00200: MMJJ_RS00200 - translation initiation factor IF-5A, at 38,158 to 38,553
_RS00200
MMJJ_RS00205: MMJJ_RS00205 - adenosylcobinamide-phosphate synthase CbiB, at 38,646 to 39,566
_RS00205
Position (kb) 37
38
39 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 36.270 kb on - strand at 36.270 kb on - strand at 36.274 kb on - strand at 36.274 kb on - strand at 36.274 kb on - strand at 36.274 kb on - strand at 36.274 kb on - strand at 36.275 kb on + strand at 36.277 kb on + strand at 36.277 kb on + strand at 36.278 kb on - strand at 36.281 kb on - strand at 36.282 kb on + strand at 36.289 kb on - strand at 36.289 kb on - strand at 36.291 kb on - strand at 36.291 kb on - strand at 36.291 kb on - strand at 36.291 kb on - strand at 36.291 kb on - strand at 36.291 kb on - strand at 36.292 kb on + strand at 36.292 kb on + strand at 36.296 kb on - strand at 36.301 kb on + strand at 36.319 kb on - strand at 36.320 kb on + strand at 36.333 kb on + strand at 36.335 kb on - strand at 36.337 kb on - strand at 36.338 kb on + strand at 36.340 kb on + strand at 36.340 kb on + strand at 36.345 kb on + strand at 36.364 kb on + strand at 36.365 kb on - strand at 36.369 kb on + strand at 36.386 kb on - strand at 36.387 kb on + strand at 36.387 kb on + strand at 36.388 kb on - strand at 36.389 kb on + strand at 36.393 kb on - strand at 36.394 kb on + strand at 36.398 kb on + strand at 36.398 kb on + strand at 36.400 kb on - strand at 36.409 kb on - strand at 36.413 kb on - strand at 36.414 kb on + strand at 36.415 kb on - strand at 36.415 kb on - strand at 36.415 kb on - strand at 36.415 kb on - strand at 36.415 kb on - strand at 36.415 kb on - strand at 36.415 kb on - strand at 36.416 kb on + strand at 36.416 kb on + strand at 36.416 kb on + strand at 36.416 kb on + strand at 36.416 kb on + strand at 36.416 kb on + strand at 36.416 kb on + strand at 36.419 kb on - strand at 36.428 kb on - strand at 36.428 kb on - strand at 36.429 kb on + strand at 36.431 kb on - strand at 36.431 kb on - strand at 36.431 kb on - strand at 36.432 kb on + strand at 36.432 kb on + strand at 36.435 kb on - strand at 36.436 kb on + strand at 36.436 kb on + strand at 36.437 kb on - strand at 36.437 kb on - strand at 36.437 kb on - strand at 36.437 kb on - strand at 36.437 kb on - strand at 36.437 kb on - strand at 36.438 kb on + strand at 36.438 kb on + strand at 36.438 kb on + strand at 36.453 kb on - strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.456 kb on - strand at 36.456 kb on - strand at 36.456 kb on - strand at 36.457 kb on + strand at 36.457 kb on + strand at 36.457 kb on + strand at 36.457 kb on + strand at 36.457 kb on + strand at 36.460 kb on - strand at 36.460 kb on - strand at 36.460 kb on - strand at 36.460 kb on - strand at 36.461 kb on + strand at 36.461 kb on + strand at 36.461 kb on + strand at 36.461 kb on + strand at 36.461 kb on + strand at 36.461 kb on + strand at 36.471 kb on + strand at 36.483 kb on + strand at 36.483 kb on + strand at 36.534 kb on - strand at 36.566 kb on + strand, within MMJJ_RS00190 at 36.574 kb on + strand, within MMJJ_RS00190 at 36.585 kb on - strand, within MMJJ_RS00190 at 36.585 kb on - strand, within MMJJ_RS00190 at 36.586 kb on + strand, within MMJJ_RS00190 at 36.587 kb on - strand, within MMJJ_RS00190 at 36.587 kb on - strand, within MMJJ_RS00190 at 36.624 kb on - strand, within MMJJ_RS00190 at 36.624 kb on - strand, within MMJJ_RS00190 at 36.625 kb on + strand, within MMJJ_RS00190 at 36.630 kb on - strand, within MMJJ_RS00190 at 36.632 kb on - strand, within MMJJ_RS00190 at 36.633 kb on + strand, within MMJJ_RS00190 at 36.646 kb on - strand, within MMJJ_RS00190 at 36.671 kb on - strand, within MMJJ_RS00190 at 36.672 kb on + strand, within MMJJ_RS00190 at 36.673 kb on - strand, within MMJJ_RS00190 at 36.701 kb on + strand, within MMJJ_RS00190 at 36.881 kb on - strand, within MMJJ_RS00190 at 36.889 kb on + strand, within MMJJ_RS00190 at 36.909 kb on + strand, within MMJJ_RS00190 at 36.909 kb on + strand, within MMJJ_RS00190 at 36.910 kb on - strand, within MMJJ_RS00190 at 36.911 kb on + strand, within MMJJ_RS00190 at 36.915 kb on + strand, within MMJJ_RS00190 at 36.961 kb on - strand at 36.962 kb on + strand at 37.013 kb on - strand at 37.013 kb on - strand at 37.013 kb on - strand at 37.017 kb on - strand at 37.017 kb on - strand at 37.017 kb on - strand at 37.019 kb on - strand at 37.020 kb on + strand at 37.020 kb on + strand at 37.020 kb on + strand at 37.021 kb on - strand at 37.022 kb on + strand at 37.034 kb on - strand at 37.034 kb on - strand at 37.035 kb on + strand at 37.035 kb on + strand at 37.035 kb on + strand at 37.035 kb on + strand at 37.036 kb on - strand at 37.037 kb on + strand at 37.037 kb on + strand at 37.037 kb on + strand at 37.037 kb on + strand at 37.049 kb on - strand at 37.050 kb on + strand at 37.052 kb on - strand at 37.053 kb on + strand at 37.053 kb on + strand at 37.053 kb on + strand at 37.076 kb on - strand at 37.076 kb on - strand at 37.076 kb on - strand at 37.096 kb on - strand at 37.107 kb on - strand at 37.107 kb on - strand at 37.108 kb on + strand at 37.176 kb on + strand at 37.189 kb on + strand at 37.198 kb on - strand at 37.201 kb on - strand at 37.202 kb on + strand at 37.204 kb on + strand at 37.214 kb on + strand at 37.240 kb on + strand at 37.269 kb on - strand at 37.270 kb on + strand at 37.346 kb on + strand, within MMJJ_RS00195 at 37.352 kb on + strand, within MMJJ_RS00195 at 37.401 kb on - strand, within MMJJ_RS00195 at 37.501 kb on - strand at 37.502 kb on + strand, within MMJJ_RS00195 at 37.502 kb on + strand, within MMJJ_RS00195 at 37.504 kb on + strand, within MMJJ_RS00195 at 37.532 kb on + strand at 37.571 kb on + strand, within MMJJ_RS00195 at 37.571 kb on + strand, within MMJJ_RS00195 at 37.606 kb on - strand, within MMJJ_RS00195 at 37.606 kb on - strand at 37.809 kb on + strand at 37.908 kb on + strand, within MMJJ_RS00195 at 37.995 kb on - strand at 37.996 kb on + strand at 37.996 kb on + strand at 37.997 kb on - strand at 37.997 kb on - strand at 37.997 kb on - strand at 37.997 kb on - strand at 37.997 kb on - strand at 37.998 kb on + strand at 37.998 kb on + strand at 37.998 kb on + strand at 38.008 kb on + strand at 38.024 kb on - strand at 38.040 kb on - strand at 38.045 kb on + strand at 38.045 kb on + strand at 38.045 kb on + strand at 38.045 kb on + strand at 38.045 kb on + strand at 38.046 kb on - strand at 38.046 kb on - strand at 38.046 kb on - strand at 38.047 kb on + strand at 38.047 kb on + strand at 38.047 kb on + strand at 38.047 kb on + strand at 38.047 kb on + strand at 38.047 kb on + strand at 38.058 kb on + strand at 38.090 kb on - strand at 38.090 kb on - strand at 38.123 kb on + strand at 38.136 kb on + strand at 38.304 kb on - strand, within MMJJ_RS00200 at 38.334 kb on + strand, within MMJJ_RS00200 at 38.551 kb on - strand at 38.580 kb on - strand at 38.665 kb on - strand at 38.665 kb on - strand at 38.755 kb on - strand, within MMJJ_RS00205 at 38.758 kb on - strand, within MMJJ_RS00205 at 38.780 kb on - strand, within MMJJ_RS00205 at 38.784 kb on - strand, within MMJJ_RS00205 at 38.903 kb on - strand, within MMJJ_RS00205 at 38.983 kb on + strand, within MMJJ_RS00205
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 36,270 - -0.3 36,270 - +0.6 36,274 - +0.8 36,274 - +0.3 36,274 - +1.1 36,274 - +0.7 36,274 - -0.3 36,275 + -1.2 36,277 + +0.9 36,277 + +0.4 36,278 - -0.1 36,281 - +0.1 36,282 + +0.3 36,289 - -0.1 36,289 - -1.1 36,291 - -0.1 36,291 - +0.4 36,291 - +0.4 36,291 - +0.0 36,291 - -0.1 36,291 - -0.2 36,292 + +0.6 36,292 + +1.2 36,296 - -0.5 36,301 + -0.3 36,319 - -0.1 36,320 + +0.1 36,333 + -0.6 36,335 - +0.7 36,337 - +0.5 36,338 + -1.3 36,340 + +0.4 36,340 + +0.3 36,345 + +0.7 36,364 + +0.6 36,365 - +0.1 36,369 + -0.1 36,386 - -1.2 36,387 + +0.7 36,387 + +0.2 36,388 - +0.1 36,389 + -0.1 36,393 - +0.0 36,394 + -0.3 36,398 + -0.9 36,398 + +1.9 36,400 - +0.4 36,409 - +0.6 36,413 - +0.0 36,414 + +0.1 36,415 - +0.7 36,415 - -0.1 36,415 - +0.4 36,415 - +0.9 36,415 - +0.6 36,415 - +1.1 36,415 - +0.3 36,416 + +0.3 36,416 + +0.3 36,416 + +0.2 36,416 + +0.5 36,416 + +0.2 36,416 + -0.3 36,416 + -0.1 36,419 - -0.6 36,428 - -0.7 36,428 - +0.4 36,429 + +0.1 36,431 - +0.1 36,431 - -0.1 36,431 - -0.2 36,432 + +1.4 36,432 + +0.4 36,435 - -0.2 36,436 + +0.0 36,436 + +0.1 36,437 - +0.7 36,437 - +1.3 36,437 - +0.2 36,437 - -0.4 36,437 - -0.2 36,437 - -0.5 36,438 + +0.8 36,438 + -0.0 36,438 + +0.8 36,453 - +0.8 36,454 + +0.6 36,454 + -0.2 36,456 - +0.2 36,456 - +1.0 36,456 - +0.1 36,457 + +0.1 36,457 + +1.0 36,457 + +0.2 36,457 + +0.8 36,457 + +0.4 36,460 - -0.5 36,460 - -1.1 36,460 - +0.3 36,460 - -0.2 36,461 + +2.0 36,461 + +1.2 36,461 + -0.6 36,461 + -0.5 36,461 + -0.3 36,461 + +0.1 36,471 + +0.6 36,483 + -0.7 36,483 + -0.5 36,534 - -0.1 36,566 + MMJJ_RS00190 0.14 -2.1 36,574 + MMJJ_RS00190 0.15 +0.2 36,585 - MMJJ_RS00190 0.18 -1.2 36,585 - MMJJ_RS00190 0.18 -0.8 36,586 + MMJJ_RS00190 0.18 +1.5 36,587 - MMJJ_RS00190 0.18 +0.5 36,587 - MMJJ_RS00190 0.18 -0.2 36,624 - MMJJ_RS00190 0.26 -0.3 36,624 - MMJJ_RS00190 0.26 -0.2 36,625 + MMJJ_RS00190 0.26 +0.6 36,630 - MMJJ_RS00190 0.27 +0.4 36,632 - MMJJ_RS00190 0.28 -0.6 36,633 + MMJJ_RS00190 0.28 -0.4 36,646 - MMJJ_RS00190 0.31 +0.1 36,671 - MMJJ_RS00190 0.36 -0.2 36,672 + MMJJ_RS00190 0.36 +0.3 36,673 - MMJJ_RS00190 0.36 +0.9 36,701 + MMJJ_RS00190 0.42 -0.6 36,881 - MMJJ_RS00190 0.80 +0.6 36,889 + MMJJ_RS00190 0.82 -0.1 36,909 + MMJJ_RS00190 0.86 -0.7 36,909 + MMJJ_RS00190 0.86 -0.1 36,910 - MMJJ_RS00190 0.86 +0.8 36,911 + MMJJ_RS00190 0.86 -0.9 36,915 + MMJJ_RS00190 0.87 +0.3 36,961 - +0.9 36,962 + +0.5 37,013 - +2.2 37,013 - +1.3 37,013 - -0.0 37,017 - +0.5 37,017 - +0.3 37,017 - +0.9 37,019 - +1.0 37,020 + +1.0 37,020 + +0.4 37,020 + -0.5 37,021 - -1.7 37,022 + -0.3 37,034 - +0.1 37,034 - +0.9 37,035 + +0.7 37,035 + +0.4 37,035 + +0.4 37,035 + +0.3 37,036 - +0.8 37,037 + -0.1 37,037 + +0.7 37,037 + +0.0 37,037 + +0.0 37,049 - +1.0 37,050 + +0.9 37,052 - -0.3 37,053 + -0.3 37,053 + -0.3 37,053 + -0.5 37,076 - +0.2 37,076 - +1.2 37,076 - +0.3 37,096 - +0.8 37,107 - +0.4 37,107 - -0.0 37,108 + -0.1 37,176 + +0.3 37,189 + -0.0 37,198 - -0.8 37,201 - -1.1 37,202 + -2.8 37,204 + +0.9 37,214 + -1.2 37,240 + +0.1 37,269 - -1.5 37,270 + -2.2 37,346 + MMJJ_RS00195 0.11 -3.1 37,352 + MMJJ_RS00195 0.12 -0.9 37,401 - MMJJ_RS00195 0.18 -0.9 37,501 - -0.4 37,502 + MMJJ_RS00195 0.32 -1.4 37,502 + MMJJ_RS00195 0.32 -1.1 37,504 + MMJJ_RS00195 0.32 -1.1 37,532 + -0.9 37,571 + MMJJ_RS00195 0.41 -1.4 37,571 + MMJJ_RS00195 0.41 -1.4 37,606 - MMJJ_RS00195 0.46 -1.7 37,606 - -1.4 37,809 + -1.1 37,908 + MMJJ_RS00195 0.86 -1.0 37,995 - -1.4 37,996 + -1.1 37,996 + +0.3 37,997 - +0.8 37,997 - +0.8 37,997 - -0.2 37,997 - +0.1 37,997 - +0.6 37,998 + +1.2 37,998 + +0.4 37,998 + -1.5 38,008 + -0.6 38,024 - +0.5 38,040 - -0.5 38,045 + +0.9 38,045 + -1.1 38,045 + -1.0 38,045 + -0.2 38,045 + -0.8 38,046 - -0.2 38,046 - +1.1 38,046 - -0.7 38,047 + -0.4 38,047 + +0.3 38,047 + -1.7 38,047 + -0.3 38,047 + +0.5 38,047 + +0.5 38,058 + +0.9 38,090 - -1.0 38,090 - +0.1 38,123 + -1.1 38,136 + -2.8 38,304 - MMJJ_RS00200 0.37 -0.7 38,334 + MMJJ_RS00200 0.44 +1.4 38,551 - +0.5 38,580 - +0.7 38,665 - -1.9 38,665 - +0.8 38,755 - MMJJ_RS00205 0.12 -3.7 38,758 - MMJJ_RS00205 0.12 -3.6 38,780 - MMJJ_RS00205 0.15 -1.5 38,784 - MMJJ_RS00205 0.15 -1.4 38,903 - MMJJ_RS00205 0.28 +0.2 38,983 + MMJJ_RS00205 0.37 -1.0
Or see this region's nucleotide sequence