Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00055 and MMJJ_RS00060 are separated by 13 nucleotides MMJJ_RS00060 and MMJJ_RS00065 are separated by 4 nucleotides MMJJ_RS00065 and MMJJ_RS00070 are separated by 104 nucleotides MMJJ_RS00070 and MMJJ_RS00075 are separated by 12 nucleotides
MMJJ_RS00055: MMJJ_RS00055 - CO dehydrogenase/acetyl-CoA synthase subunit delta, at 11,442 to 12,596
_RS00055
MMJJ_RS00060: MMJJ_RS00060 - acetyl-CoA decarbonylase/synthase complex subunit gamma, at 12,610 to 13,983
_RS00060
MMJJ_RS00065: MMJJ_RS00065 - 4Fe-4S dicluster domain-containing protein, at 13,988 to 14,452
_RS00065
MMJJ_RS00070: MMJJ_RS00070 - CooT family nickel-binding protein, at 14,557 to 14,736
_RS00070
MMJJ_RS00075: MMJJ_RS00075 - AAA family ATPase, at 14,749 to 15,531
_RS00075
Position (kb) 12
13
14 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 11.629 kb on + strand, within MMJJ_RS00055 at 11.888 kb on - strand, within MMJJ_RS00055 at 11.888 kb on - strand, within MMJJ_RS00055 at 11.928 kb on + strand, within MMJJ_RS00055 at 11.978 kb on + strand, within MMJJ_RS00055 at 12.169 kb on - strand, within MMJJ_RS00055 at 12.170 kb on + strand, within MMJJ_RS00055 at 12.170 kb on + strand, within MMJJ_RS00055 at 12.170 kb on + strand, within MMJJ_RS00055 at 12.170 kb on + strand, within MMJJ_RS00055 at 12.174 kb on + strand, within MMJJ_RS00055 at 12.174 kb on + strand, within MMJJ_RS00055 at 12.233 kb on + strand, within MMJJ_RS00055 at 12.233 kb on + strand, within MMJJ_RS00055 at 12.410 kb on - strand, within MMJJ_RS00055 at 12.428 kb on - strand, within MMJJ_RS00055 at 12.452 kb on - strand, within MMJJ_RS00055 at 12.453 kb on + strand, within MMJJ_RS00055 at 12.478 kb on - strand, within MMJJ_RS00055 at 12.478 kb on - strand, within MMJJ_RS00055 at 12.478 kb on - strand, within MMJJ_RS00055 at 12.479 kb on + strand, within MMJJ_RS00055 at 12.506 kb on - strand at 12.506 kb on - strand at 12.524 kb on + strand at 12.541 kb on + strand at 12.587 kb on - strand at 12.588 kb on + strand at 12.630 kb on - strand at 12.631 kb on + strand at 12.641 kb on - strand at 12.648 kb on - strand at 12.648 kb on - strand at 12.684 kb on - strand at 12.685 kb on + strand at 12.685 kb on + strand at 12.685 kb on + strand at 12.745 kb on + strand at 12.792 kb on + strand, within MMJJ_RS00060 at 12.817 kb on + strand, within MMJJ_RS00060 at 12.829 kb on + strand, within MMJJ_RS00060 at 12.848 kb on - strand, within MMJJ_RS00060 at 12.851 kb on - strand, within MMJJ_RS00060 at 12.853 kb on - strand, within MMJJ_RS00060 at 12.854 kb on + strand, within MMJJ_RS00060 at 12.854 kb on + strand, within MMJJ_RS00060 at 12.854 kb on + strand, within MMJJ_RS00060 at 12.854 kb on + strand, within MMJJ_RS00060 at 12.859 kb on - strand, within MMJJ_RS00060 at 12.859 kb on - strand, within MMJJ_RS00060 at 12.868 kb on + strand, within MMJJ_RS00060 at 12.868 kb on + strand, within MMJJ_RS00060 at 12.871 kb on - strand, within MMJJ_RS00060 at 12.871 kb on - strand, within MMJJ_RS00060 at 12.871 kb on - strand, within MMJJ_RS00060 at 12.872 kb on + strand, within MMJJ_RS00060 at 12.874 kb on - strand, within MMJJ_RS00060 at 12.890 kb on - strand, within MMJJ_RS00060 at 12.902 kb on - strand, within MMJJ_RS00060 at 13.001 kb on - strand, within MMJJ_RS00060 at 13.002 kb on + strand, within MMJJ_RS00060 at 13.014 kb on - strand, within MMJJ_RS00060 at 13.153 kb on - strand, within MMJJ_RS00060 at 13.173 kb on - strand, within MMJJ_RS00060 at 13.295 kb on - strand, within MMJJ_RS00060 at 13.298 kb on - strand, within MMJJ_RS00060 at 13.298 kb on - strand, within MMJJ_RS00060 at 13.298 kb on - strand, within MMJJ_RS00060 at 13.299 kb on + strand at 13.299 kb on + strand, within MMJJ_RS00060 at 13.299 kb on + strand, within MMJJ_RS00060 at 13.299 kb on + strand, within MMJJ_RS00060 at 13.301 kb on - strand, within MMJJ_RS00060 at 13.302 kb on + strand, within MMJJ_RS00060 at 13.315 kb on - strand, within MMJJ_RS00060 at 13.315 kb on - strand, within MMJJ_RS00060 at 13.339 kb on + strand, within MMJJ_RS00060 at 13.362 kb on + strand, within MMJJ_RS00060 at 13.458 kb on + strand, within MMJJ_RS00060 at 13.507 kb on - strand, within MMJJ_RS00060 at 13.507 kb on - strand, within MMJJ_RS00060 at 13.507 kb on - strand, within MMJJ_RS00060 at 13.508 kb on + strand, within MMJJ_RS00060 at 13.517 kb on - strand, within MMJJ_RS00060 at 13.517 kb on - strand, within MMJJ_RS00060 at 13.518 kb on + strand, within MMJJ_RS00060 at 13.540 kb on + strand, within MMJJ_RS00060 at 13.580 kb on - strand, within MMJJ_RS00060 at 13.668 kb on - strand, within MMJJ_RS00060 at 13.669 kb on + strand, within MMJJ_RS00060 at 13.683 kb on + strand, within MMJJ_RS00060 at 13.687 kb on - strand, within MMJJ_RS00060 at 13.688 kb on + strand, within MMJJ_RS00060 at 13.690 kb on - strand, within MMJJ_RS00060 at 13.690 kb on - strand, within MMJJ_RS00060 at 13.691 kb on + strand, within MMJJ_RS00060 at 13.691 kb on + strand, within MMJJ_RS00060 at 13.736 kb on - strand, within MMJJ_RS00060 at 13.737 kb on + strand, within MMJJ_RS00060 at 13.737 kb on + strand, within MMJJ_RS00060 at 13.750 kb on + strand, within MMJJ_RS00060 at 13.818 kb on - strand, within MMJJ_RS00060 at 13.925 kb on - strand at 13.925 kb on - strand at 13.925 kb on - strand at 13.925 kb on - strand at 13.925 kb on - strand at 13.925 kb on - strand at 13.926 kb on + strand at 13.926 kb on + strand at 13.968 kb on + strand at 14.005 kb on + strand at 14.041 kb on - strand, within MMJJ_RS00065 at 14.041 kb on - strand, within MMJJ_RS00065 at 14.043 kb on - strand, within MMJJ_RS00065 at 14.053 kb on - strand, within MMJJ_RS00065 at 14.054 kb on + strand, within MMJJ_RS00065 at 14.080 kb on - strand, within MMJJ_RS00065 at 14.081 kb on + strand, within MMJJ_RS00065 at 14.081 kb on + strand, within MMJJ_RS00065 at 14.083 kb on - strand, within MMJJ_RS00065 at 14.140 kb on - strand, within MMJJ_RS00065 at 14.140 kb on - strand, within MMJJ_RS00065 at 14.140 kb on - strand, within MMJJ_RS00065 at 14.140 kb on - strand, within MMJJ_RS00065 at 14.140 kb on - strand, within MMJJ_RS00065 at 14.148 kb on - strand, within MMJJ_RS00065 at 14.148 kb on - strand, within MMJJ_RS00065 at 14.148 kb on - strand, within MMJJ_RS00065 at 14.149 kb on + strand, within MMJJ_RS00065 at 14.157 kb on - strand, within MMJJ_RS00065 at 14.175 kb on - strand, within MMJJ_RS00065 at 14.176 kb on + strand, within MMJJ_RS00065 at 14.190 kb on - strand, within MMJJ_RS00065 at 14.211 kb on - strand, within MMJJ_RS00065 at 14.242 kb on - strand, within MMJJ_RS00065 at 14.254 kb on - strand at 14.290 kb on - strand, within MMJJ_RS00065 at 14.312 kb on + strand, within MMJJ_RS00065 at 14.341 kb on - strand, within MMJJ_RS00065 at 14.341 kb on - strand, within MMJJ_RS00065 at 14.361 kb on - strand, within MMJJ_RS00065 at 14.389 kb on - strand, within MMJJ_RS00065 at 14.390 kb on + strand, within MMJJ_RS00065 at 14.450 kb on - strand at 14.450 kb on - strand at 14.474 kb on - strand at 14.484 kb on - strand at 14.485 kb on + strand at 14.496 kb on - strand at 14.496 kb on - strand at 14.504 kb on - strand at 14.509 kb on - strand at 14.516 kb on - strand at 14.540 kb on - strand at 14.556 kb on - strand at 14.556 kb on - strand at 14.557 kb on + strand at 14.557 kb on + strand at 14.589 kb on - strand, within MMJJ_RS00070 at 14.594 kb on - strand, within MMJJ_RS00070 at 14.595 kb on + strand, within MMJJ_RS00070 at 14.717 kb on - strand, within MMJJ_RS00070 at 14.735 kb on + strand at 14.822 kb on + strand at 14.854 kb on - strand, within MMJJ_RS00075 at 14.855 kb on + strand, within MMJJ_RS00075 at 14.855 kb on + strand, within MMJJ_RS00075 at 14.963 kb on + strand, within MMJJ_RS00075
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 11,629 + MMJJ_RS00055 0.16 -2.0 11,888 - MMJJ_RS00055 0.39 -2.1 11,888 - MMJJ_RS00055 0.39 -1.9 11,928 + MMJJ_RS00055 0.42 -1.0 11,978 + MMJJ_RS00055 0.46 -0.6 12,169 - MMJJ_RS00055 0.63 -2.1 12,170 + MMJJ_RS00055 0.63 -3.3 12,170 + MMJJ_RS00055 0.63 -2.6 12,170 + MMJJ_RS00055 0.63 -2.7 12,170 + MMJJ_RS00055 0.63 -1.9 12,174 + MMJJ_RS00055 0.63 -3.1 12,174 + MMJJ_RS00055 0.63 -4.4 12,233 + MMJJ_RS00055 0.68 -2.1 12,233 + MMJJ_RS00055 0.68 -2.1 12,410 - MMJJ_RS00055 0.84 -3.7 12,428 - MMJJ_RS00055 0.85 -3.1 12,452 - MMJJ_RS00055 0.87 -2.7 12,453 + MMJJ_RS00055 0.88 -1.8 12,478 - MMJJ_RS00055 0.90 -1.1 12,478 - MMJJ_RS00055 0.90 -1.9 12,478 - MMJJ_RS00055 0.90 -1.7 12,479 + MMJJ_RS00055 0.90 -1.4 12,506 - -1.5 12,506 - -2.2 12,524 + -3.2 12,541 + -3.4 12,587 - -1.5 12,588 + -0.7 12,630 - -0.3 12,631 + -2.7 12,641 - -1.9 12,648 - -3.5 12,648 - -1.7 12,684 - -3.1 12,685 + -3.0 12,685 + -3.1 12,685 + -1.7 12,745 + -0.4 12,792 + MMJJ_RS00060 0.13 -2.0 12,817 + MMJJ_RS00060 0.15 -2.5 12,829 + MMJJ_RS00060 0.16 -2.4 12,848 - MMJJ_RS00060 0.17 -2.7 12,851 - MMJJ_RS00060 0.18 -2.6 12,853 - MMJJ_RS00060 0.18 -3.5 12,854 + MMJJ_RS00060 0.18 -2.3 12,854 + MMJJ_RS00060 0.18 -3.5 12,854 + MMJJ_RS00060 0.18 -2.5 12,854 + MMJJ_RS00060 0.18 -1.6 12,859 - MMJJ_RS00060 0.18 -2.8 12,859 - MMJJ_RS00060 0.18 -3.3 12,868 + MMJJ_RS00060 0.19 -3.4 12,868 + MMJJ_RS00060 0.19 -1.4 12,871 - MMJJ_RS00060 0.19 -2.7 12,871 - MMJJ_RS00060 0.19 -2.9 12,871 - MMJJ_RS00060 0.19 -1.5 12,872 + MMJJ_RS00060 0.19 -1.3 12,874 - MMJJ_RS00060 0.19 -3.0 12,890 - MMJJ_RS00060 0.20 -1.8 12,902 - MMJJ_RS00060 0.21 -1.6 13,001 - MMJJ_RS00060 0.28 -1.8 13,002 + MMJJ_RS00060 0.29 -2.8 13,014 - MMJJ_RS00060 0.29 -2.2 13,153 - MMJJ_RS00060 0.40 -1.9 13,173 - MMJJ_RS00060 0.41 -1.4 13,295 - MMJJ_RS00060 0.50 -3.3 13,298 - MMJJ_RS00060 0.50 -2.7 13,298 - MMJJ_RS00060 0.50 -2.2 13,298 - MMJJ_RS00060 0.50 -1.3 13,299 + +0.3 13,299 + MMJJ_RS00060 0.50 -1.4 13,299 + MMJJ_RS00060 0.50 -1.6 13,299 + MMJJ_RS00060 0.50 -3.1 13,301 - MMJJ_RS00060 0.50 -3.8 13,302 + MMJJ_RS00060 0.50 -1.4 13,315 - MMJJ_RS00060 0.51 -2.7 13,315 - MMJJ_RS00060 0.51 -1.5 13,339 + MMJJ_RS00060 0.53 -2.2 13,362 + MMJJ_RS00060 0.55 -1.7 13,458 + MMJJ_RS00060 0.62 -3.8 13,507 - MMJJ_RS00060 0.65 -2.9 13,507 - MMJJ_RS00060 0.65 -3.0 13,507 - MMJJ_RS00060 0.65 -1.7 13,508 + MMJJ_RS00060 0.65 -3.0 13,517 - MMJJ_RS00060 0.66 -3.3 13,517 - MMJJ_RS00060 0.66 -1.3 13,518 + MMJJ_RS00060 0.66 -2.4 13,540 + MMJJ_RS00060 0.68 -3.2 13,580 - MMJJ_RS00060 0.71 -2.7 13,668 - MMJJ_RS00060 0.77 -2.1 13,669 + MMJJ_RS00060 0.77 -2.9 13,683 + MMJJ_RS00060 0.78 -3.0 13,687 - MMJJ_RS00060 0.78 -1.7 13,688 + MMJJ_RS00060 0.78 -1.9 13,690 - MMJJ_RS00060 0.79 -2.8 13,690 - MMJJ_RS00060 0.79 -1.3 13,691 + MMJJ_RS00060 0.79 -2.4 13,691 + MMJJ_RS00060 0.79 -1.0 13,736 - MMJJ_RS00060 0.82 -2.1 13,737 + MMJJ_RS00060 0.82 -1.8 13,737 + MMJJ_RS00060 0.82 -2.0 13,750 + MMJJ_RS00060 0.83 -2.2 13,818 - MMJJ_RS00060 0.88 -3.9 13,925 - -3.8 13,925 - -2.2 13,925 - -1.7 13,925 - -3.8 13,925 - -4.3 13,925 - -2.5 13,926 + -1.2 13,926 + -3.2 13,968 + -3.4 14,005 + -1.9 14,041 - MMJJ_RS00065 0.11 -2.3 14,041 - MMJJ_RS00065 0.11 -1.3 14,043 - MMJJ_RS00065 0.12 -2.5 14,053 - MMJJ_RS00065 0.14 -0.8 14,054 + MMJJ_RS00065 0.14 -2.5 14,080 - MMJJ_RS00065 0.20 -1.9 14,081 + MMJJ_RS00065 0.20 -2.3 14,081 + MMJJ_RS00065 0.20 -1.7 14,083 - MMJJ_RS00065 0.20 -2.7 14,140 - MMJJ_RS00065 0.33 -2.0 14,140 - MMJJ_RS00065 0.33 -2.3 14,140 - MMJJ_RS00065 0.33 -2.3 14,140 - MMJJ_RS00065 0.33 -1.9 14,140 - MMJJ_RS00065 0.33 -2.4 14,148 - MMJJ_RS00065 0.34 -1.8 14,148 - MMJJ_RS00065 0.34 -3.2 14,148 - MMJJ_RS00065 0.34 -0.7 14,149 + MMJJ_RS00065 0.35 -2.9 14,157 - MMJJ_RS00065 0.36 -0.5 14,175 - MMJJ_RS00065 0.40 -1.4 14,176 + MMJJ_RS00065 0.40 -3.2 14,190 - MMJJ_RS00065 0.43 -2.1 14,211 - MMJJ_RS00065 0.48 -2.2 14,242 - MMJJ_RS00065 0.55 -1.9 14,254 - -0.7 14,290 - MMJJ_RS00065 0.65 -2.8 14,312 + MMJJ_RS00065 0.70 -1.7 14,341 - MMJJ_RS00065 0.76 -1.6 14,341 - MMJJ_RS00065 0.76 -2.3 14,361 - MMJJ_RS00065 0.80 -1.4 14,389 - MMJJ_RS00065 0.86 -2.1 14,390 + MMJJ_RS00065 0.86 +1.1 14,450 - -0.8 14,450 - -1.6 14,474 - -1.1 14,484 - -1.6 14,485 + -0.5 14,496 - -0.7 14,496 - -0.7 14,504 - -1.2 14,509 - -1.9 14,516 - -2.5 14,540 - -1.5 14,556 - -4.0 14,556 - -1.7 14,557 + -2.3 14,557 + -1.1 14,589 - MMJJ_RS00070 0.18 -2.1 14,594 - MMJJ_RS00070 0.21 -1.4 14,595 + MMJJ_RS00070 0.21 -4.0 14,717 - MMJJ_RS00070 0.89 -1.8 14,735 + -0.8 14,822 + -0.4 14,854 - MMJJ_RS00075 0.13 -2.9 14,855 + MMJJ_RS00075 0.14 -3.1 14,855 + MMJJ_RS00075 0.14 -1.4 14,963 + MMJJ_RS00075 0.27 -2.6
Or see this region's nucleotide sequence