Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00045 and MMJJ_RS00050 are separated by 10 nucleotides MMJJ_RS00050 and MMJJ_RS00055 are separated by 14 nucleotides MMJJ_RS00055 and MMJJ_RS00060 are separated by 13 nucleotides
MMJJ_RS00045: MMJJ_RS00045 - CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta, at 9,291 to 10,664
_RS00045
MMJJ_RS00050: MMJJ_RS00050 - AAA family ATPase, at 10,675 to 11,427
_RS00050
MMJJ_RS00055: MMJJ_RS00055 - CO dehydrogenase/acetyl-CoA synthase subunit delta, at 11,442 to 12,596
_RS00055
MMJJ_RS00060: MMJJ_RS00060 - acetyl-CoA decarbonylase/synthase complex subunit gamma, at 12,610 to 13,983
_RS00060
Position (kb) 11
12
13 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 10.611 kb on + strand at 10.612 kb on - strand at 10.613 kb on + strand at 10.652 kb on + strand at 10.652 kb on + strand at 10.731 kb on + strand at 10.775 kb on - strand, within MMJJ_RS00050 at 10.775 kb on - strand, within MMJJ_RS00050 at 10.776 kb on + strand, within MMJJ_RS00050 at 10.869 kb on - strand, within MMJJ_RS00050 at 10.882 kb on + strand, within MMJJ_RS00050 at 10.882 kb on + strand, within MMJJ_RS00050 at 10.914 kb on + strand, within MMJJ_RS00050 at 10.914 kb on + strand, within MMJJ_RS00050 at 10.940 kb on - strand, within MMJJ_RS00050 at 10.997 kb on + strand, within MMJJ_RS00050 at 11.050 kb on - strand, within MMJJ_RS00050 at 11.060 kb on - strand, within MMJJ_RS00050 at 11.061 kb on + strand, within MMJJ_RS00050 at 11.126 kb on - strand, within MMJJ_RS00050 at 11.127 kb on + strand, within MMJJ_RS00050 at 11.127 kb on + strand, within MMJJ_RS00050 at 11.133 kb on + strand, within MMJJ_RS00050 at 11.228 kb on - strand, within MMJJ_RS00050 at 11.235 kb on - strand, within MMJJ_RS00050 at 11.235 kb on - strand, within MMJJ_RS00050 at 11.299 kb on + strand, within MMJJ_RS00050 at 11.310 kb on + strand, within MMJJ_RS00050 at 11.335 kb on - strand, within MMJJ_RS00050 at 11.497 kb on - strand at 11.518 kb on - strand at 11.519 kb on + strand at 11.533 kb on - strand at 11.540 kb on + strand at 11.540 kb on + strand at 11.546 kb on - strand at 11.552 kb on + strand at 11.629 kb on + strand, within MMJJ_RS00055 at 11.888 kb on - strand, within MMJJ_RS00055 at 11.888 kb on - strand, within MMJJ_RS00055 at 11.928 kb on + strand, within MMJJ_RS00055 at 11.978 kb on + strand, within MMJJ_RS00055 at 12.169 kb on - strand, within MMJJ_RS00055 at 12.170 kb on + strand, within MMJJ_RS00055 at 12.170 kb on + strand, within MMJJ_RS00055 at 12.170 kb on + strand, within MMJJ_RS00055 at 12.170 kb on + strand, within MMJJ_RS00055 at 12.174 kb on + strand, within MMJJ_RS00055 at 12.174 kb on + strand, within MMJJ_RS00055 at 12.233 kb on + strand, within MMJJ_RS00055 at 12.233 kb on + strand, within MMJJ_RS00055 at 12.410 kb on - strand, within MMJJ_RS00055 at 12.428 kb on - strand, within MMJJ_RS00055 at 12.452 kb on - strand, within MMJJ_RS00055 at 12.453 kb on + strand, within MMJJ_RS00055 at 12.478 kb on - strand, within MMJJ_RS00055 at 12.478 kb on - strand, within MMJJ_RS00055 at 12.478 kb on - strand, within MMJJ_RS00055 at 12.479 kb on + strand, within MMJJ_RS00055 at 12.506 kb on - strand at 12.506 kb on - strand at 12.524 kb on + strand at 12.541 kb on + strand at 12.587 kb on - strand at 12.588 kb on + strand at 12.630 kb on - strand at 12.631 kb on + strand at 12.641 kb on - strand at 12.648 kb on - strand at 12.648 kb on - strand at 12.684 kb on - strand at 12.685 kb on + strand at 12.685 kb on + strand at 12.685 kb on + strand at 12.745 kb on + strand at 12.792 kb on + strand, within MMJJ_RS00060 at 12.817 kb on + strand, within MMJJ_RS00060 at 12.829 kb on + strand, within MMJJ_RS00060 at 12.848 kb on - strand, within MMJJ_RS00060 at 12.851 kb on - strand, within MMJJ_RS00060 at 12.853 kb on - strand, within MMJJ_RS00060 at 12.854 kb on + strand, within MMJJ_RS00060 at 12.854 kb on + strand, within MMJJ_RS00060 at 12.854 kb on + strand, within MMJJ_RS00060 at 12.854 kb on + strand, within MMJJ_RS00060 at 12.859 kb on - strand, within MMJJ_RS00060 at 12.859 kb on - strand, within MMJJ_RS00060 at 12.868 kb on + strand, within MMJJ_RS00060 at 12.868 kb on + strand, within MMJJ_RS00060 at 12.871 kb on - strand, within MMJJ_RS00060 at 12.871 kb on - strand, within MMJJ_RS00060 at 12.871 kb on - strand, within MMJJ_RS00060 at 12.872 kb on + strand, within MMJJ_RS00060 at 12.874 kb on - strand, within MMJJ_RS00060 at 12.890 kb on - strand, within MMJJ_RS00060 at 12.902 kb on - strand, within MMJJ_RS00060 at 13.001 kb on - strand, within MMJJ_RS00060 at 13.002 kb on + strand, within MMJJ_RS00060 at 13.014 kb on - strand, within MMJJ_RS00060 at 13.153 kb on - strand, within MMJJ_RS00060 at 13.173 kb on - strand, within MMJJ_RS00060 at 13.295 kb on - strand, within MMJJ_RS00060 at 13.298 kb on - strand, within MMJJ_RS00060 at 13.298 kb on - strand, within MMJJ_RS00060 at 13.298 kb on - strand, within MMJJ_RS00060 at 13.299 kb on + strand at 13.299 kb on + strand, within MMJJ_RS00060 at 13.299 kb on + strand, within MMJJ_RS00060 at 13.299 kb on + strand, within MMJJ_RS00060 at 13.301 kb on - strand, within MMJJ_RS00060 at 13.302 kb on + strand, within MMJJ_RS00060 at 13.315 kb on - strand, within MMJJ_RS00060 at 13.315 kb on - strand, within MMJJ_RS00060 at 13.339 kb on + strand, within MMJJ_RS00060 at 13.362 kb on + strand, within MMJJ_RS00060 at 13.458 kb on + strand, within MMJJ_RS00060 at 13.507 kb on - strand, within MMJJ_RS00060 at 13.507 kb on - strand, within MMJJ_RS00060 at 13.507 kb on - strand, within MMJJ_RS00060 at 13.508 kb on + strand, within MMJJ_RS00060 at 13.517 kb on - strand, within MMJJ_RS00060 at 13.517 kb on - strand, within MMJJ_RS00060 at 13.518 kb on + strand, within MMJJ_RS00060 at 13.540 kb on + strand, within MMJJ_RS00060 at 13.580 kb on - strand, within MMJJ_RS00060
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 10,611 + -3.0 10,612 - +0.9 10,613 + -2.1 10,652 + -3.2 10,652 + -1.4 10,731 + -1.4 10,775 - MMJJ_RS00050 0.13 -3.1 10,775 - MMJJ_RS00050 0.13 -1.8 10,776 + MMJJ_RS00050 0.13 -1.9 10,869 - MMJJ_RS00050 0.26 -1.9 10,882 + MMJJ_RS00050 0.27 -2.8 10,882 + MMJJ_RS00050 0.27 -1.9 10,914 + MMJJ_RS00050 0.32 -0.7 10,914 + MMJJ_RS00050 0.32 -1.5 10,940 - MMJJ_RS00050 0.35 -2.3 10,997 + MMJJ_RS00050 0.43 -2.4 11,050 - MMJJ_RS00050 0.50 -1.2 11,060 - MMJJ_RS00050 0.51 -2.6 11,061 + MMJJ_RS00050 0.51 -0.8 11,126 - MMJJ_RS00050 0.60 -3.6 11,127 + MMJJ_RS00050 0.60 -3.1 11,127 + MMJJ_RS00050 0.60 -0.8 11,133 + MMJJ_RS00050 0.61 -2.1 11,228 - MMJJ_RS00050 0.73 -3.0 11,235 - MMJJ_RS00050 0.74 -2.3 11,235 - MMJJ_RS00050 0.74 -2.8 11,299 + MMJJ_RS00050 0.83 -3.0 11,310 + MMJJ_RS00050 0.84 -2.6 11,335 - MMJJ_RS00050 0.88 -1.5 11,497 - -2.0 11,518 - -2.3 11,519 + -4.2 11,533 - -1.4 11,540 + -2.8 11,540 + -2.1 11,546 - -4.2 11,552 + -2.1 11,629 + MMJJ_RS00055 0.16 -2.0 11,888 - MMJJ_RS00055 0.39 -2.1 11,888 - MMJJ_RS00055 0.39 -1.9 11,928 + MMJJ_RS00055 0.42 -1.0 11,978 + MMJJ_RS00055 0.46 -0.6 12,169 - MMJJ_RS00055 0.63 -2.1 12,170 + MMJJ_RS00055 0.63 -3.3 12,170 + MMJJ_RS00055 0.63 -2.6 12,170 + MMJJ_RS00055 0.63 -2.7 12,170 + MMJJ_RS00055 0.63 -1.9 12,174 + MMJJ_RS00055 0.63 -3.1 12,174 + MMJJ_RS00055 0.63 -4.4 12,233 + MMJJ_RS00055 0.68 -2.1 12,233 + MMJJ_RS00055 0.68 -2.1 12,410 - MMJJ_RS00055 0.84 -3.7 12,428 - MMJJ_RS00055 0.85 -3.1 12,452 - MMJJ_RS00055 0.87 -2.7 12,453 + MMJJ_RS00055 0.88 -1.8 12,478 - MMJJ_RS00055 0.90 -1.1 12,478 - MMJJ_RS00055 0.90 -1.9 12,478 - MMJJ_RS00055 0.90 -1.7 12,479 + MMJJ_RS00055 0.90 -1.4 12,506 - -1.5 12,506 - -2.2 12,524 + -3.2 12,541 + -3.4 12,587 - -1.5 12,588 + -0.7 12,630 - -0.3 12,631 + -2.7 12,641 - -1.9 12,648 - -3.5 12,648 - -1.7 12,684 - -3.1 12,685 + -3.0 12,685 + -3.1 12,685 + -1.7 12,745 + -0.4 12,792 + MMJJ_RS00060 0.13 -2.0 12,817 + MMJJ_RS00060 0.15 -2.5 12,829 + MMJJ_RS00060 0.16 -2.4 12,848 - MMJJ_RS00060 0.17 -2.7 12,851 - MMJJ_RS00060 0.18 -2.6 12,853 - MMJJ_RS00060 0.18 -3.5 12,854 + MMJJ_RS00060 0.18 -2.3 12,854 + MMJJ_RS00060 0.18 -3.5 12,854 + MMJJ_RS00060 0.18 -2.5 12,854 + MMJJ_RS00060 0.18 -1.6 12,859 - MMJJ_RS00060 0.18 -2.8 12,859 - MMJJ_RS00060 0.18 -3.3 12,868 + MMJJ_RS00060 0.19 -3.4 12,868 + MMJJ_RS00060 0.19 -1.4 12,871 - MMJJ_RS00060 0.19 -2.7 12,871 - MMJJ_RS00060 0.19 -2.9 12,871 - MMJJ_RS00060 0.19 -1.5 12,872 + MMJJ_RS00060 0.19 -1.3 12,874 - MMJJ_RS00060 0.19 -3.0 12,890 - MMJJ_RS00060 0.20 -1.8 12,902 - MMJJ_RS00060 0.21 -1.6 13,001 - MMJJ_RS00060 0.28 -1.8 13,002 + MMJJ_RS00060 0.29 -2.8 13,014 - MMJJ_RS00060 0.29 -2.2 13,153 - MMJJ_RS00060 0.40 -1.9 13,173 - MMJJ_RS00060 0.41 -1.4 13,295 - MMJJ_RS00060 0.50 -3.3 13,298 - MMJJ_RS00060 0.50 -2.7 13,298 - MMJJ_RS00060 0.50 -2.2 13,298 - MMJJ_RS00060 0.50 -1.3 13,299 + +0.3 13,299 + MMJJ_RS00060 0.50 -1.4 13,299 + MMJJ_RS00060 0.50 -1.6 13,299 + MMJJ_RS00060 0.50 -3.1 13,301 - MMJJ_RS00060 0.50 -3.8 13,302 + MMJJ_RS00060 0.50 -1.4 13,315 - MMJJ_RS00060 0.51 -2.7 13,315 - MMJJ_RS00060 0.51 -1.5 13,339 + MMJJ_RS00060 0.53 -2.2 13,362 + MMJJ_RS00060 0.55 -1.7 13,458 + MMJJ_RS00060 0.62 -3.8 13,507 - MMJJ_RS00060 0.65 -2.9 13,507 - MMJJ_RS00060 0.65 -3.0 13,507 - MMJJ_RS00060 0.65 -1.7 13,508 + MMJJ_RS00060 0.65 -3.0 13,517 - MMJJ_RS00060 0.66 -3.3 13,517 - MMJJ_RS00060 0.66 -1.3 13,518 + MMJJ_RS00060 0.66 -2.4 13,540 + MMJJ_RS00060 0.68 -3.2 13,580 - MMJJ_RS00060 0.71 -2.7
Or see this region's nucleotide sequence