Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00035 and MMJJ_RS00040 are separated by 9 nucleotides MMJJ_RS00040 and MMJJ_RS00045 are separated by 11 nucleotides MMJJ_RS00045 and MMJJ_RS00050 are separated by 10 nucleotides MMJJ_RS00050 and MMJJ_RS00055 are separated by 14 nucleotides
MMJJ_RS00035: MMJJ_RS00035 - CO dehydrogenase/acetyl-CoA synthase complex subunit alpha, at 6,409 to 8,751
_RS00035
MMJJ_RS00040: MMJJ_RS00040 - CO dehydrogenase/acetyl-CoA synthase complex subunit epsilon, at 8,761 to 9,279
_RS00040
MMJJ_RS00045: MMJJ_RS00045 - CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta, at 9,291 to 10,664
_RS00045
MMJJ_RS00050: MMJJ_RS00050 - AAA family ATPase, at 10,675 to 11,427
_RS00050
MMJJ_RS00055: MMJJ_RS00055 - CO dehydrogenase/acetyl-CoA synthase subunit delta, at 11,442 to 12,596
_RS00055
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 8.335 kb on + strand, within MMJJ_RS00035 at 8.380 kb on - strand, within MMJJ_RS00035 at 8.380 kb on - strand, within MMJJ_RS00035 at 8.426 kb on - strand, within MMJJ_RS00035 at 8.507 kb on - strand, within MMJJ_RS00035 at 8.507 kb on - strand, within MMJJ_RS00035 at 8.508 kb on + strand, within MMJJ_RS00035 at 8.528 kb on - strand at 8.547 kb on - strand at 8.562 kb on + strand at 8.579 kb on - strand at 8.579 kb on - strand at 8.579 kb on - strand at 8.580 kb on + strand at 8.585 kb on - strand at 8.596 kb on - strand at 8.622 kb on + strand at 8.627 kb on - strand at 8.628 kb on + strand at 8.749 kb on - strand at 8.772 kb on - strand at 8.773 kb on + strand at 8.799 kb on + strand at 8.799 kb on + strand at 8.829 kb on + strand, within MMJJ_RS00040 at 8.897 kb on - strand, within MMJJ_RS00040 at 8.954 kb on - strand, within MMJJ_RS00040 at 8.955 kb on + strand, within MMJJ_RS00040 at 9.038 kb on - strand, within MMJJ_RS00040 at 9.039 kb on + strand, within MMJJ_RS00040 at 9.118 kb on - strand, within MMJJ_RS00040 at 9.118 kb on - strand, within MMJJ_RS00040 at 9.118 kb on - strand, within MMJJ_RS00040 at 9.119 kb on + strand, within MMJJ_RS00040 at 9.119 kb on + strand, within MMJJ_RS00040 at 9.121 kb on - strand, within MMJJ_RS00040 at 9.122 kb on + strand, within MMJJ_RS00040 at 9.122 kb on + strand, within MMJJ_RS00040 at 9.122 kb on + strand, within MMJJ_RS00040 at 9.194 kb on + strand, within MMJJ_RS00040 at 9.194 kb on + strand, within MMJJ_RS00040 at 9.228 kb on - strand at 9.228 kb on - strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.253 kb on - strand at 9.304 kb on - strand at 9.305 kb on + strand at 9.316 kb on - strand at 9.317 kb on + strand at 9.327 kb on - strand at 9.327 kb on - strand at 9.328 kb on + strand at 9.407 kb on + strand at 9.422 kb on + strand at 9.545 kb on + strand, within MMJJ_RS00045 at 9.576 kb on - strand, within MMJJ_RS00045 at 9.576 kb on - strand, within MMJJ_RS00045 at 9.576 kb on - strand, within MMJJ_RS00045 at 9.591 kb on + strand, within MMJJ_RS00045 at 9.598 kb on - strand, within MMJJ_RS00045 at 9.599 kb on + strand, within MMJJ_RS00045 at 9.599 kb on + strand, within MMJJ_RS00045 at 9.661 kb on - strand, within MMJJ_RS00045 at 9.736 kb on - strand, within MMJJ_RS00045 at 9.736 kb on - strand, within MMJJ_RS00045 at 9.757 kb on - strand, within MMJJ_RS00045 at 9.807 kb on + strand, within MMJJ_RS00045 at 9.842 kb on + strand, within MMJJ_RS00045 at 9.846 kb on - strand, within MMJJ_RS00045 at 9.847 kb on + strand, within MMJJ_RS00045 at 9.855 kb on + strand, within MMJJ_RS00045 at 9.892 kb on - strand, within MMJJ_RS00045 at 9.944 kb on - strand, within MMJJ_RS00045 at 10.027 kb on + strand, within MMJJ_RS00045 at 10.031 kb on - strand, within MMJJ_RS00045 at 10.031 kb on - strand, within MMJJ_RS00045 at 10.032 kb on + strand, within MMJJ_RS00045 at 10.055 kb on - strand, within MMJJ_RS00045 at 10.067 kb on - strand, within MMJJ_RS00045 at 10.068 kb on + strand, within MMJJ_RS00045 at 10.082 kb on + strand, within MMJJ_RS00045 at 10.084 kb on + strand, within MMJJ_RS00045 at 10.087 kb on - strand, within MMJJ_RS00045 at 10.088 kb on + strand, within MMJJ_RS00045 at 10.138 kb on - strand, within MMJJ_RS00045 at 10.146 kb on - strand, within MMJJ_RS00045 at 10.199 kb on - strand, within MMJJ_RS00045 at 10.200 kb on + strand, within MMJJ_RS00045 at 10.281 kb on + strand, within MMJJ_RS00045 at 10.281 kb on + strand, within MMJJ_RS00045 at 10.287 kb on - strand, within MMJJ_RS00045 at 10.287 kb on - strand, within MMJJ_RS00045 at 10.288 kb on + strand, within MMJJ_RS00045 at 10.288 kb on + strand, within MMJJ_RS00045 at 10.288 kb on + strand, within MMJJ_RS00045 at 10.289 kb on - strand, within MMJJ_RS00045 at 10.290 kb on + strand, within MMJJ_RS00045 at 10.358 kb on + strand, within MMJJ_RS00045 at 10.365 kb on + strand, within MMJJ_RS00045 at 10.393 kb on - strand, within MMJJ_RS00045 at 10.393 kb on - strand, within MMJJ_RS00045 at 10.393 kb on - strand, within MMJJ_RS00045 at 10.611 kb on + strand at 10.612 kb on - strand at 10.613 kb on + strand at 10.652 kb on + strand at 10.652 kb on + strand at 10.731 kb on + strand at 10.775 kb on - strand, within MMJJ_RS00050 at 10.775 kb on - strand, within MMJJ_RS00050 at 10.776 kb on + strand, within MMJJ_RS00050 at 10.869 kb on - strand, within MMJJ_RS00050 at 10.882 kb on + strand, within MMJJ_RS00050 at 10.882 kb on + strand, within MMJJ_RS00050 at 10.914 kb on + strand, within MMJJ_RS00050 at 10.914 kb on + strand, within MMJJ_RS00050 at 10.940 kb on - strand, within MMJJ_RS00050 at 10.997 kb on + strand, within MMJJ_RS00050 at 11.050 kb on - strand, within MMJJ_RS00050 at 11.060 kb on - strand, within MMJJ_RS00050 at 11.061 kb on + strand, within MMJJ_RS00050 at 11.126 kb on - strand, within MMJJ_RS00050 at 11.127 kb on + strand, within MMJJ_RS00050 at 11.127 kb on + strand, within MMJJ_RS00050 at 11.133 kb on + strand, within MMJJ_RS00050 at 11.228 kb on - strand, within MMJJ_RS00050 at 11.235 kb on - strand, within MMJJ_RS00050 at 11.235 kb on - strand, within MMJJ_RS00050 at 11.299 kb on + strand, within MMJJ_RS00050 at 11.310 kb on + strand, within MMJJ_RS00050 at 11.335 kb on - strand, within MMJJ_RS00050 at 11.497 kb on - strand at 11.518 kb on - strand at 11.519 kb on + strand at 11.533 kb on - strand at 11.540 kb on + strand at 11.540 kb on + strand at 11.546 kb on - strand at 11.552 kb on + strand at 11.629 kb on + strand, within MMJJ_RS00055
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 8,335 + MMJJ_RS00035 0.82 -0.3 8,380 - MMJJ_RS00035 0.84 -2.1 8,380 - MMJJ_RS00035 0.84 -0.8 8,426 - MMJJ_RS00035 0.86 -2.3 8,507 - MMJJ_RS00035 0.90 -2.4 8,507 - MMJJ_RS00035 0.90 -2.8 8,508 + MMJJ_RS00035 0.90 -3.2 8,528 - -1.2 8,547 - -0.7 8,562 + -3.3 8,579 - -3.5 8,579 - -2.2 8,579 - -2.3 8,580 + -1.5 8,585 - -2.4 8,596 - -1.8 8,622 + -2.6 8,627 - -2.3 8,628 + -2.3 8,749 - -2.8 8,772 - -1.3 8,773 + -4.0 8,799 + -2.6 8,799 + -2.8 8,829 + MMJJ_RS00040 0.13 -2.8 8,897 - MMJJ_RS00040 0.26 -3.0 8,954 - MMJJ_RS00040 0.37 -2.3 8,955 + MMJJ_RS00040 0.37 -2.0 9,038 - MMJJ_RS00040 0.53 -2.1 9,039 + MMJJ_RS00040 0.54 -2.1 9,118 - MMJJ_RS00040 0.69 -1.5 9,118 - MMJJ_RS00040 0.69 -1.9 9,118 - MMJJ_RS00040 0.69 -1.1 9,119 + MMJJ_RS00040 0.69 -5.4 9,119 + MMJJ_RS00040 0.69 -1.9 9,121 - MMJJ_RS00040 0.69 -3.5 9,122 + MMJJ_RS00040 0.70 +0.3 9,122 + MMJJ_RS00040 0.70 -2.3 9,122 + MMJJ_RS00040 0.70 -1.7 9,194 + MMJJ_RS00040 0.83 -3.4 9,194 + MMJJ_RS00040 0.83 -1.5 9,228 - -2.5 9,228 - -1.3 9,229 + -4.5 9,229 + -2.0 9,229 + -3.4 9,229 + -2.6 9,229 + -2.3 9,253 - -2.7 9,304 - -2.8 9,305 + -2.3 9,316 - -1.5 9,317 + -1.5 9,327 - -1.9 9,327 - -2.0 9,328 + -1.6 9,407 + -3.5 9,422 + -3.7 9,545 + MMJJ_RS00045 0.18 -2.8 9,576 - MMJJ_RS00045 0.21 -2.1 9,576 - MMJJ_RS00045 0.21 -2.5 9,576 - MMJJ_RS00045 0.21 -1.7 9,591 + MMJJ_RS00045 0.22 -1.5 9,598 - MMJJ_RS00045 0.22 +1.3 9,599 + MMJJ_RS00045 0.22 -2.2 9,599 + MMJJ_RS00045 0.22 -3.4 9,661 - MMJJ_RS00045 0.27 -1.6 9,736 - MMJJ_RS00045 0.32 +0.0 9,736 - MMJJ_RS00045 0.32 -1.0 9,757 - MMJJ_RS00045 0.34 -4.8 9,807 + MMJJ_RS00045 0.38 -1.3 9,842 + MMJJ_RS00045 0.40 -2.3 9,846 - MMJJ_RS00045 0.40 -1.3 9,847 + MMJJ_RS00045 0.40 -0.7 9,855 + MMJJ_RS00045 0.41 -2.9 9,892 - MMJJ_RS00045 0.44 -3.4 9,944 - MMJJ_RS00045 0.48 -2.2 10,027 + MMJJ_RS00045 0.54 -1.8 10,031 - MMJJ_RS00045 0.54 -0.7 10,031 - MMJJ_RS00045 0.54 -2.7 10,032 + MMJJ_RS00045 0.54 -2.1 10,055 - MMJJ_RS00045 0.56 -1.7 10,067 - MMJJ_RS00045 0.56 -1.8 10,068 + MMJJ_RS00045 0.57 -3.2 10,082 + MMJJ_RS00045 0.58 -0.9 10,084 + MMJJ_RS00045 0.58 -1.7 10,087 - MMJJ_RS00045 0.58 -2.4 10,088 + MMJJ_RS00045 0.58 -0.7 10,138 - MMJJ_RS00045 0.62 -2.5 10,146 - MMJJ_RS00045 0.62 -1.7 10,199 - MMJJ_RS00045 0.66 -1.8 10,200 + MMJJ_RS00045 0.66 -1.0 10,281 + MMJJ_RS00045 0.72 -0.3 10,281 + MMJJ_RS00045 0.72 -3.5 10,287 - MMJJ_RS00045 0.72 -1.3 10,287 - MMJJ_RS00045 0.72 -1.8 10,288 + MMJJ_RS00045 0.73 -1.2 10,288 + MMJJ_RS00045 0.73 -2.2 10,288 + MMJJ_RS00045 0.73 -1.9 10,289 - MMJJ_RS00045 0.73 -1.5 10,290 + MMJJ_RS00045 0.73 -3.6 10,358 + MMJJ_RS00045 0.78 -2.4 10,365 + MMJJ_RS00045 0.78 -1.6 10,393 - MMJJ_RS00045 0.80 -2.1 10,393 - MMJJ_RS00045 0.80 -1.5 10,393 - MMJJ_RS00045 0.80 -3.5 10,611 + -3.0 10,612 - +0.9 10,613 + -2.1 10,652 + -3.2 10,652 + -1.4 10,731 + -1.4 10,775 - MMJJ_RS00050 0.13 -3.1 10,775 - MMJJ_RS00050 0.13 -1.8 10,776 + MMJJ_RS00050 0.13 -1.9 10,869 - MMJJ_RS00050 0.26 -1.9 10,882 + MMJJ_RS00050 0.27 -2.8 10,882 + MMJJ_RS00050 0.27 -1.9 10,914 + MMJJ_RS00050 0.32 -0.7 10,914 + MMJJ_RS00050 0.32 -1.5 10,940 - MMJJ_RS00050 0.35 -2.3 10,997 + MMJJ_RS00050 0.43 -2.4 11,050 - MMJJ_RS00050 0.50 -1.2 11,060 - MMJJ_RS00050 0.51 -2.6 11,061 + MMJJ_RS00050 0.51 -0.8 11,126 - MMJJ_RS00050 0.60 -3.6 11,127 + MMJJ_RS00050 0.60 -3.1 11,127 + MMJJ_RS00050 0.60 -0.8 11,133 + MMJJ_RS00050 0.61 -2.1 11,228 - MMJJ_RS00050 0.73 -3.0 11,235 - MMJJ_RS00050 0.74 -2.3 11,235 - MMJJ_RS00050 0.74 -2.8 11,299 + MMJJ_RS00050 0.83 -3.0 11,310 + MMJJ_RS00050 0.84 -2.6 11,335 - MMJJ_RS00050 0.88 -1.5 11,497 - -2.0 11,518 - -2.3 11,519 + -4.2 11,533 - -1.4 11,540 + -2.8 11,540 + -2.1 11,546 - -4.2 11,552 + -2.1 11,629 + MMJJ_RS00055 0.16 -2.0
Or see this region's nucleotide sequence