Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_25450

Experiment: Spectinomycin 0.2 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.2 mg/ml
5,609,980	+		AO353_25440	0.90	+1.4
5,609,980	+		AO353_25440	0.90	+0.1
5,609,980	+		AO353_25440	0.90	+1.1
5,609,980	+		AO353_25440	0.90	-0.5
5,609,980	+		AO353_25440	0.90	+0.6
5,609,980	+		AO353_25440	0.90	-0.3
5,609,981	-		AO353_25440	0.90	-0.0
5,609,981	-		AO353_25440	0.90	-0.2
5,609,981	-		AO353_25440	0.90	+0.5
5,609,981	-		AO353_25440	0.90	-0.6
5,609,981	-		AO353_25440	0.90	+1.1
5,609,981	-		AO353_25440	0.90	-0.1
5,609,986	+				-1.6
5,609,986	+				-0.0
5,609,987	-				-0.8
5,609,994	-				+1.2
5,610,075	+				-0.9
5,610,076	-				-1.2
5,610,121	+				-1.6
5,610,122	-				-2.4
5,610,122	-				-0.8
5,610,122	-				+1.0
5,610,122	-				+0.3
5,610,126	+				-0.4
5,610,126	+				+0.8
5,610,127	-				-0.0
5,610,144	+				-0.1
5,610,146	+				-0.1
5,610,159	+				+3.8
5,610,169	-				-0.2
5,610,191	+				-0.6
5,610,191	+				-0.7
5,610,191	+				-0.3
5,610,192	-				+1.3
5,610,192	-				+0.5
5,610,192	-				+0.2
5,610,192	-				-0.0
5,610,234	+				-0.0
5,610,234	+				-1.1
5,610,235	-				-0.3
5,610,235	-				-0.4
5,610,338	+		AO353_25445	0.14	-0.9
5,610,338	+		AO353_25445	0.14	+1.3
5,610,338	+		AO353_25445	0.14	+1.5
5,610,339	-		AO353_25445	0.14	+1.0
5,610,339	-		AO353_25445	0.14	-1.9
5,610,339	-		AO353_25445	0.14	-0.8
5,610,520	+		AO353_25445	0.42	+0.5
5,610,520	+		AO353_25445	0.42	-2.1
5,610,520	+		AO353_25445	0.42	-0.9
5,610,521	-		AO353_25445	0.42	+0.0
5,610,521	-		AO353_25445	0.42	+0.4
5,610,616	-		AO353_25445	0.56	-0.8
5,610,787	-		AO353_25445	0.82	+0.8
5,610,958	+				+0.4
5,610,959	-				-2.5
5,610,959	-				-0.6
5,611,064	-		AO353_25450	0.26	-1.8
5,611,064	-		AO353_25450	0.26	+1.6
5,611,064	-		AO353_25450	0.26	+1.5
5,611,222	+		AO353_25450	0.60	+0.3
5,611,223	-		AO353_25450	0.60	+0.5
5,611,223	-		AO353_25450	0.60	-1.1
5,611,396	+				-0.0
5,611,396	+				-0.2
5,611,396	+				-0.3
5,611,397	-				-1.1
5,611,397	-				+1.4
5,611,408	+				+0.6
5,611,408	+				+0.4
5,611,409	-				-1.4
5,611,433	+				+1.2
5,611,486	-				-0.2
5,611,486	-				-0.1
5,611,486	-				-0.6
5,611,487	+				-1.0
5,611,487	+				+0.3
5,611,487	+				-0.2
5,611,488	-				+0.6
5,611,488	-				-2.1
5,611,488	-				+0.5
5,611,488	-				-0.8
5,611,506	+				-0.5
5,611,506	+				+1.5
5,611,506	+				+2.2
5,611,507	-				-0.9
5,611,507	-				-1.5
5,611,507	-				+1.1
5,611,507	-				+0.1
5,611,613	+				+2.8
5,611,613	+		AO353_25455	0.48	-0.1
5,611,613	+		AO353_25455	0.48	-1.6
5,611,614	-		AO353_25455	0.49	+2.0
5,611,614	-		AO353_25455	0.49	-0.0
5,611,778	+				-0.7
5,611,778	+				+0.7
5,611,778	+				-0.3
5,611,779	-				+0.8
5,611,779	-				-0.2
5,611,779	-				+0.4
5,611,779	-				+1.7
5,611,779	-				+0.2
5,611,779	-				+0.2
5,611,779	-				-2.5
5,611,779	-				-1.6
5,611,779	-				-0.6
5,611,791	+				-0.7
5,611,791	+				-0.5
5,611,792	-				-0.1
5,612,128	+				-3.3
5,612,128	+				-2.4
5,612,128	+				-0.7
5,612,128	+				+0.5
5,612,129	-				-1.1
5,612,129	-				-0.8