Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_22100 and AO353_22105 are separated by 2 nucleotides AO353_22105 and AO353_22110 overlap by 8 nucleotides AO353_22110 and AO353_22115 are separated by 10 nucleotides AO353_22115 and AO353_22120 are separated by 25 nucleotides
AO353_22100: AO353_22100 - nitrate reductase, at 4,842,357 to 4,843,895
_22100
AO353_22105: AO353_22105 - nitrate reductase, at 4,843,898 to 4,844,653
_22105
AO353_22110: AO353_22110 - nitrate reductase, at 4,844,646 to 4,845,326
_22110
AO353_22115: AO353_22115 - peptidylprolyl isomerase, at 4,845,337 to 4,846,296
_22115
AO353_22120: AO353_22120 - cyclic pyranopterin phosphate synthase MoaA, at 4,846,322 to 4,847,314
_22120
Position (kb)
4844
4845
4846 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4843.683 kb on + strand, within AO353_22100 at 4843.683 kb on + strand, within AO353_22100 at 4843.683 kb on + strand, within AO353_22100 at 4843.684 kb on - strand, within AO353_22100 at 4843.689 kb on + strand, within AO353_22100 at 4843.707 kb on + strand, within AO353_22100 at 4843.707 kb on + strand, within AO353_22100 at 4843.707 kb on + strand, within AO353_22100 at 4843.707 kb on + strand, within AO353_22100 at 4843.707 kb on + strand, within AO353_22100 at 4844.081 kb on + strand, within AO353_22105 at 4844.190 kb on - strand, within AO353_22105 at 4844.234 kb on + strand, within AO353_22105 at 4844.234 kb on + strand, within AO353_22105 at 4844.234 kb on + strand, within AO353_22105 at 4844.234 kb on + strand, within AO353_22105 at 4844.234 kb on + strand at 4844.234 kb on + strand, within AO353_22105 at 4844.235 kb on - strand, within AO353_22105 at 4844.235 kb on - strand, within AO353_22105 at 4844.235 kb on - strand, within AO353_22105 at 4844.235 kb on - strand, within AO353_22105 at 4844.235 kb on - strand, within AO353_22105 at 4844.235 kb on - strand, within AO353_22105 at 4844.235 kb on - strand, within AO353_22105 at 4844.236 kb on + strand, within AO353_22105 at 4844.237 kb on - strand, within AO353_22105 at 4844.358 kb on - strand, within AO353_22105 at 4844.640 kb on + strand at 4844.640 kb on + strand at 4844.641 kb on - strand at 4844.651 kb on + strand at 4844.651 kb on + strand at 4844.651 kb on + strand at 4844.652 kb on - strand at 4844.652 kb on - strand at 4844.679 kb on + strand at 4844.679 kb on + strand at 4844.679 kb on + strand at 4844.680 kb on - strand at 4844.680 kb on - strand at 4844.680 kb on - strand at 4844.680 kb on - strand at 4844.680 kb on - strand at 4844.680 kb on - strand at 4844.685 kb on + strand at 4844.685 kb on + strand at 4844.685 kb on + strand at 4844.685 kb on + strand at 4844.685 kb on + strand at 4844.685 kb on + strand at 4844.686 kb on - strand at 4844.686 kb on - strand at 4844.686 kb on - strand at 4844.686 kb on - strand at 4844.686 kb on - strand at 4844.699 kb on + strand at 4844.700 kb on - strand at 4844.743 kb on - strand, within AO353_22110 at 4844.743 kb on - strand, within AO353_22110 at 4844.743 kb on - strand, within AO353_22110 at 4844.743 kb on - strand, within AO353_22110 at 4844.875 kb on - strand, within AO353_22110 at 4844.960 kb on - strand, within AO353_22110 at 4844.960 kb on - strand, within AO353_22110 at 4845.198 kb on + strand, within AO353_22110 at 4845.198 kb on + strand, within AO353_22110 at 4845.198 kb on + strand, within AO353_22110 at 4845.199 kb on - strand, within AO353_22110 at 4845.199 kb on - strand, within AO353_22110 at 4845.199 kb on - strand, within AO353_22110 at 4845.199 kb on - strand, within AO353_22110 at 4845.199 kb on - strand, within AO353_22110 at 4845.259 kb on + strand at 4845.259 kb on + strand at 4845.259 kb on + strand at 4845.259 kb on + strand at 4845.260 kb on - strand at 4845.260 kb on - strand at 4845.285 kb on + strand at 4845.285 kb on + strand at 4845.285 kb on + strand at 4845.285 kb on + strand at 4845.286 kb on - strand at 4845.583 kb on + strand, within AO353_22115 at 4845.584 kb on - strand, within AO353_22115 at 4845.584 kb on - strand, within AO353_22115 at 4845.787 kb on + strand at 4845.790 kb on + strand, within AO353_22115 at 4845.964 kb on + strand, within AO353_22115 at 4846.028 kb on + strand, within AO353_22115 at 4846.029 kb on - strand, within AO353_22115 at 4846.029 kb on - strand, within AO353_22115 at 4846.078 kb on - strand, within AO353_22115 at 4846.078 kb on - strand, within AO353_22115 at 4846.220 kb on - strand at 4846.220 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 4,843,683 + AO353_22100 0.86 +1.7 4,843,683 + AO353_22100 0.86 +0.6 4,843,683 + AO353_22100 0.86 -0.1 4,843,684 - AO353_22100 0.86 -0.1 4,843,689 + AO353_22100 0.87 -0.1 4,843,707 + AO353_22100 0.88 +1.2 4,843,707 + AO353_22100 0.88 +1.1 4,843,707 + AO353_22100 0.88 +1.1 4,843,707 + AO353_22100 0.88 -1.0 4,843,707 + AO353_22100 0.88 -1.0 4,844,081 + AO353_22105 0.24 +0.1 4,844,190 - AO353_22105 0.39 -1.3 4,844,234 + AO353_22105 0.44 +1.6 4,844,234 + AO353_22105 0.44 -0.8 4,844,234 + AO353_22105 0.44 -1.1 4,844,234 + AO353_22105 0.44 -1.1 4,844,234 + +1.3 4,844,234 + AO353_22105 0.44 +0.0 4,844,235 - AO353_22105 0.45 +0.5 4,844,235 - AO353_22105 0.45 -1.6 4,844,235 - AO353_22105 0.45 -1.1 4,844,235 - AO353_22105 0.45 -2.5 4,844,235 - AO353_22105 0.45 +1.5 4,844,235 - AO353_22105 0.45 -1.8 4,844,235 - AO353_22105 0.45 +0.6 4,844,236 + AO353_22105 0.45 +0.9 4,844,237 - AO353_22105 0.45 +0.5 4,844,358 - AO353_22105 0.61 +0.3 4,844,640 + +0.3 4,844,640 + -1.5 4,844,641 - -0.0 4,844,651 + +0.6 4,844,651 + -0.3 4,844,651 + -1.5 4,844,652 - +1.6 4,844,652 - +0.9 4,844,679 + +1.5 4,844,679 + -0.3 4,844,679 + -0.8 4,844,680 - +0.1 4,844,680 - +0.1 4,844,680 - -1.5 4,844,680 - +1.0 4,844,680 - -1.9 4,844,680 - +1.5 4,844,685 + +1.0 4,844,685 + -2.2 4,844,685 + -0.4 4,844,685 + +0.8 4,844,685 + -0.5 4,844,685 + -0.8 4,844,686 - +0.5 4,844,686 - +0.3 4,844,686 - +0.3 4,844,686 - -2.2 4,844,686 - -0.2 4,844,699 + -0.0 4,844,700 - -0.7 4,844,743 - AO353_22110 0.14 +0.9 4,844,743 - AO353_22110 0.14 -0.3 4,844,743 - AO353_22110 0.14 -1.3 4,844,743 - AO353_22110 0.14 +0.5 4,844,875 - AO353_22110 0.34 -0.9 4,844,960 - AO353_22110 0.46 -1.3 4,844,960 - AO353_22110 0.46 -0.8 4,845,198 + AO353_22110 0.81 -1.0 4,845,198 + AO353_22110 0.81 +1.3 4,845,198 + AO353_22110 0.81 -1.0 4,845,199 - AO353_22110 0.81 +0.7 4,845,199 - AO353_22110 0.81 -0.6 4,845,199 - AO353_22110 0.81 -0.3 4,845,199 - AO353_22110 0.81 -1.7 4,845,199 - AO353_22110 0.81 -0.7 4,845,259 + +1.7 4,845,259 + -2.1 4,845,259 + -0.2 4,845,259 + +0.4 4,845,260 - -1.0 4,845,260 - -0.7 4,845,285 + +1.8 4,845,285 + +0.8 4,845,285 + -0.2 4,845,285 + +1.1 4,845,286 - +0.3 4,845,583 + AO353_22115 0.26 +1.4 4,845,584 - AO353_22115 0.26 -1.7 4,845,584 - AO353_22115 0.26 -0.3 4,845,787 + +1.9 4,845,790 + AO353_22115 0.47 -0.5 4,845,964 + AO353_22115 0.65 +0.4 4,846,028 + AO353_22115 0.72 -1.2 4,846,029 - AO353_22115 0.72 -1.5 4,846,029 - AO353_22115 0.72 -0.1 4,846,078 - AO353_22115 0.77 -0.6 4,846,078 - AO353_22115 0.77 -0.7 4,846,220 - -0.7 4,846,220 - -0.3
Or see this region's nucleotide sequence