Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_22035 and AO353_22040 are separated by 100 nucleotides AO353_22040 and AO353_22045 overlap by 4 nucleotides AO353_22045 and AO353_22050 are separated by 214 nucleotides
AO353_22035: AO353_22035 - pyridoxine 5'-phosphate oxidase, at 4,825,997 to 4,826,644
_22035
AO353_22040: AO353_22040 - molybdenum cofactor biosynthesis protein B, at 4,826,745 to 4,827,299
_22040
AO353_22045: AO353_22045 - molybdenum cofactor biosynthesis protein MoaA, at 4,827,296 to 4,828,531
_22045
AO353_22050: AO353_22050 - protease, at 4,828,746 to 4,829,741
_22050
Position (kb)
4827
4828
4829 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4826.326 kb on + strand, within AO353_22035 at 4826.326 kb on + strand, within AO353_22035 at 4826.326 kb on + strand, within AO353_22035 at 4826.326 kb on + strand, within AO353_22035 at 4826.326 kb on + strand, within AO353_22035 at 4826.372 kb on + strand, within AO353_22035 at 4826.372 kb on + strand, within AO353_22035 at 4826.373 kb on - strand, within AO353_22035 at 4826.650 kb on + strand at 4826.650 kb on + strand at 4826.650 kb on + strand at 4826.650 kb on + strand at 4826.651 kb on - strand at 4826.667 kb on + strand at 4826.683 kb on + strand at 4826.683 kb on + strand at 4826.683 kb on + strand at 4826.683 kb on + strand at 4826.683 kb on + strand at 4826.684 kb on - strand at 4826.684 kb on - strand at 4826.728 kb on + strand at 4826.728 kb on + strand at 4826.728 kb on + strand at 4826.807 kb on + strand, within AO353_22040 at 4826.906 kb on + strand, within AO353_22040 at 4826.906 kb on + strand, within AO353_22040 at 4826.906 kb on + strand, within AO353_22040 at 4826.906 kb on + strand, within AO353_22040 at 4826.906 kb on + strand, within AO353_22040 at 4826.906 kb on + strand, within AO353_22040 at 4826.906 kb on + strand, within AO353_22040 at 4826.906 kb on + strand, within AO353_22040 at 4826.906 kb on + strand, within AO353_22040 at 4826.907 kb on - strand, within AO353_22040 at 4826.907 kb on - strand, within AO353_22040 at 4826.907 kb on - strand, within AO353_22040 at 4826.907 kb on - strand, within AO353_22040 at 4826.910 kb on + strand, within AO353_22040 at 4826.910 kb on + strand, within AO353_22040 at 4826.911 kb on - strand, within AO353_22040 at 4826.911 kb on - strand, within AO353_22040 at 4826.911 kb on - strand, within AO353_22040 at 4826.911 kb on - strand, within AO353_22040 at 4826.929 kb on + strand, within AO353_22040 at 4826.929 kb on + strand, within AO353_22040 at 4826.930 kb on - strand, within AO353_22040 at 4826.965 kb on + strand, within AO353_22040 at 4826.966 kb on - strand, within AO353_22040 at 4826.966 kb on - strand, within AO353_22040 at 4826.966 kb on - strand, within AO353_22040 at 4826.966 kb on - strand, within AO353_22040 at 4826.966 kb on - strand, within AO353_22040 at 4827.005 kb on + strand, within AO353_22040 at 4827.006 kb on - strand, within AO353_22040 at 4827.488 kb on + strand, within AO353_22045 at 4827.718 kb on + strand, within AO353_22045 at 4827.718 kb on + strand, within AO353_22045 at 4827.719 kb on - strand, within AO353_22045 at 4827.754 kb on - strand, within AO353_22045 at 4827.813 kb on + strand, within AO353_22045 at 4827.911 kb on + strand, within AO353_22045 at 4827.911 kb on + strand, within AO353_22045 at 4827.911 kb on + strand, within AO353_22045 at 4827.912 kb on - strand, within AO353_22045 at 4827.912 kb on - strand, within AO353_22045 at 4827.912 kb on - strand, within AO353_22045 at 4827.974 kb on + strand, within AO353_22045 at 4827.974 kb on + strand, within AO353_22045 at 4827.975 kb on - strand, within AO353_22045 at 4828.071 kb on + strand, within AO353_22045 at 4828.072 kb on - strand, within AO353_22045 at 4828.625 kb on + strand at 4828.626 kb on - strand at 4828.741 kb on + strand at 4828.741 kb on + strand at 4828.784 kb on - strand at 4828.818 kb on + strand at 4828.819 kb on - strand at 4828.819 kb on - strand at 4828.929 kb on + strand, within AO353_22050 at 4828.930 kb on - strand, within AO353_22050 at 4829.050 kb on - strand, within AO353_22050 at 4829.130 kb on + strand, within AO353_22050 at 4829.131 kb on - strand, within AO353_22050 at 4829.131 kb on - strand, within AO353_22050 at 4829.131 kb on - strand, within AO353_22050 at 4829.131 kb on - strand, within AO353_22050 at 4829.191 kb on + strand, within AO353_22050 at 4829.192 kb on - strand, within AO353_22050 at 4829.192 kb on - strand, within AO353_22050 at 4829.286 kb on + strand, within AO353_22050 at 4829.286 kb on + strand, within AO353_22050 at 4829.286 kb on + strand, within AO353_22050 at 4829.287 kb on - strand, within AO353_22050 at 4829.287 kb on - strand, within AO353_22050 at 4829.287 kb on - strand, within AO353_22050 at 4829.287 kb on - strand, within AO353_22050 at 4829.287 kb on - strand, within AO353_22050 at 4829.287 kb on - strand, within AO353_22050 at 4829.287 kb on - strand, within AO353_22050 at 4829.403 kb on + strand, within AO353_22050 at 4829.405 kb on + strand, within AO353_22050 at 4829.406 kb on - strand, within AO353_22050
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 4,826,326 + AO353_22035 0.51 -0.5 4,826,326 + AO353_22035 0.51 -1.3 4,826,326 + AO353_22035 0.51 +0.1 4,826,326 + AO353_22035 0.51 +0.5 4,826,326 + AO353_22035 0.51 +0.1 4,826,372 + AO353_22035 0.58 -1.0 4,826,372 + AO353_22035 0.58 +0.9 4,826,373 - AO353_22035 0.58 +0.6 4,826,650 + -0.0 4,826,650 + -0.0 4,826,650 + -1.8 4,826,650 + +0.2 4,826,651 - +1.9 4,826,667 + +0.3 4,826,683 + +0.9 4,826,683 + +0.7 4,826,683 + -0.4 4,826,683 + -1.3 4,826,683 + +1.6 4,826,684 - -0.1 4,826,684 - +0.7 4,826,728 + +1.1 4,826,728 + +1.3 4,826,728 + +0.6 4,826,807 + AO353_22040 0.11 -0.3 4,826,906 + AO353_22040 0.29 -0.2 4,826,906 + AO353_22040 0.29 -0.4 4,826,906 + AO353_22040 0.29 +0.9 4,826,906 + AO353_22040 0.29 -0.0 4,826,906 + AO353_22040 0.29 +0.3 4,826,906 + AO353_22040 0.29 +0.0 4,826,906 + AO353_22040 0.29 +1.3 4,826,906 + AO353_22040 0.29 -0.1 4,826,906 + AO353_22040 0.29 -0.3 4,826,907 - AO353_22040 0.29 +0.6 4,826,907 - AO353_22040 0.29 -0.1 4,826,907 - AO353_22040 0.29 +1.8 4,826,907 - AO353_22040 0.29 +0.2 4,826,910 + AO353_22040 0.30 +1.4 4,826,910 + AO353_22040 0.30 +0.8 4,826,911 - AO353_22040 0.30 +0.2 4,826,911 - AO353_22040 0.30 +1.3 4,826,911 - AO353_22040 0.30 -0.7 4,826,911 - AO353_22040 0.30 -0.5 4,826,929 + AO353_22040 0.33 +1.9 4,826,929 + AO353_22040 0.33 -0.6 4,826,930 - AO353_22040 0.33 -1.3 4,826,965 + AO353_22040 0.40 +0.5 4,826,966 - AO353_22040 0.40 -0.7 4,826,966 - AO353_22040 0.40 -0.7 4,826,966 - AO353_22040 0.40 -2.3 4,826,966 - AO353_22040 0.40 -0.2 4,826,966 - AO353_22040 0.40 +0.9 4,827,005 + AO353_22040 0.47 +1.1 4,827,006 - AO353_22040 0.47 -0.3 4,827,488 + AO353_22045 0.16 +0.2 4,827,718 + AO353_22045 0.34 +1.3 4,827,718 + AO353_22045 0.34 +0.7 4,827,719 - AO353_22045 0.34 -0.8 4,827,754 - AO353_22045 0.37 +0.1 4,827,813 + AO353_22045 0.42 +1.1 4,827,911 + AO353_22045 0.50 +0.2 4,827,911 + AO353_22045 0.50 -1.8 4,827,911 + AO353_22045 0.50 -0.0 4,827,912 - AO353_22045 0.50 +2.0 4,827,912 - AO353_22045 0.50 +1.3 4,827,912 - AO353_22045 0.50 +1.3 4,827,974 + AO353_22045 0.55 -0.0 4,827,974 + AO353_22045 0.55 +0.9 4,827,975 - AO353_22045 0.55 +1.6 4,828,071 + AO353_22045 0.63 +0.6 4,828,072 - AO353_22045 0.63 -1.2 4,828,625 + -1.9 4,828,626 - -1.2 4,828,741 + +0.4 4,828,741 + -1.7 4,828,784 - +0.6 4,828,818 + +0.4 4,828,819 - +0.0 4,828,819 - +0.9 4,828,929 + AO353_22050 0.18 -2.0 4,828,930 - AO353_22050 0.18 -1.4 4,829,050 - AO353_22050 0.31 -1.5 4,829,130 + AO353_22050 0.39 +1.3 4,829,131 - AO353_22050 0.39 +0.1 4,829,131 - AO353_22050 0.39 +0.6 4,829,131 - AO353_22050 0.39 +1.6 4,829,131 - AO353_22050 0.39 -0.9 4,829,191 + AO353_22050 0.45 -0.8 4,829,192 - AO353_22050 0.45 -2.0 4,829,192 - AO353_22050 0.45 -1.0 4,829,286 + AO353_22050 0.54 +1.3 4,829,286 + AO353_22050 0.54 -0.0 4,829,286 + AO353_22050 0.54 +0.3 4,829,287 - AO353_22050 0.54 +0.3 4,829,287 - AO353_22050 0.54 -0.7 4,829,287 - AO353_22050 0.54 +1.9 4,829,287 - AO353_22050 0.54 -0.8 4,829,287 - AO353_22050 0.54 -0.7 4,829,287 - AO353_22050 0.54 +0.4 4,829,287 - AO353_22050 0.54 -0.8 4,829,403 + AO353_22050 0.66 +0.7 4,829,405 + AO353_22050 0.66 -0.2 4,829,406 - AO353_22050 0.66 -0.7
Or see this region's nucleotide sequence