Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_20560 and AO353_20565 are separated by 189 nucleotides AO353_20565 and AO353_20570 are separated by 79 nucleotides AO353_20570 and AO353_20575 are separated by 53 nucleotides AO353_20575 and AO353_20580 are separated by 80 nucleotides AO353_20580 and AO353_20585 are separated by 182 nucleotides
AO353_20560: AO353_20560 - glutamate--tRNA ligase, at 4,513,006 to 4,514,487
_20560
AO353_20565: AO353_20565 - tRNA-Ala, at 4,514,677 to 4,514,752
_20565
AO353_20570: AO353_20570 - tRNA-Glu, at 4,514,832 to 4,514,907
_20570
AO353_20575: AO353_20575 - tRNA-Ala, at 4,514,961 to 4,515,036
_20575
AO353_20580: AO353_20580 - tRNA-Glu, at 4,515,117 to 4,515,192
_20580
AO353_20585: AO353_20585 - TetR family transcriptional regulator, at 4,515,375 to 4,515,914
_20585
Position (kb)
4514
4515 Strain fitness (log2 ratio)
-2
-1
0
1 at 4514.490 kb on + strand at 4514.490 kb on + strand at 4514.490 kb on + strand at 4514.490 kb on + strand at 4514.490 kb on + strand at 4514.491 kb on - strand at 4514.577 kb on + strand at 4514.684 kb on + strand at 4514.742 kb on + strand, within AO353_20565 at 4514.742 kb on + strand, within AO353_20565 at 4514.756 kb on + strand at 4514.912 kb on + strand at 4514.912 kb on + strand at 4515.026 kb on + strand, within AO353_20575 at 4515.082 kb on + strand at 4515.082 kb on + strand at 4515.215 kb on + strand at 4515.215 kb on + strand at 4515.215 kb on + strand at 4515.215 kb on + strand at 4515.215 kb on + strand at 4515.215 kb on + strand at 4515.215 kb on + strand at 4515.215 kb on + strand at 4515.215 kb on + strand at 4515.215 kb on + strand at 4515.217 kb on + strand at 4515.217 kb on + strand at 4515.217 kb on + strand at 4515.458 kb on + strand, within AO353_20585 at 4515.491 kb on + strand, within AO353_20585 at 4515.492 kb on - strand, within AO353_20585 at 4515.507 kb on + strand, within AO353_20585 at 4515.507 kb on + strand, within AO353_20585 at 4515.507 kb on + strand, within AO353_20585 at 4515.507 kb on + strand, within AO353_20585 at 4515.508 kb on - strand at 4515.642 kb on + strand, within AO353_20585 at 4515.642 kb on + strand, within AO353_20585
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 4,514,490 + +0.2 4,514,490 + -0.2 4,514,490 + +0.2 4,514,490 + +0.4 4,514,490 + +0.6 4,514,491 - +0.7 4,514,577 + -1.4 4,514,684 + +0.2 4,514,742 + AO353_20565 0.86 -1.0 4,514,742 + AO353_20565 0.86 -1.5 4,514,756 + -0.8 4,514,912 + -1.4 4,514,912 + -0.9 4,515,026 + AO353_20575 0.86 +0.1 4,515,082 + +0.2 4,515,082 + -0.4 4,515,215 + -0.3 4,515,215 + +0.4 4,515,215 + +0.4 4,515,215 + -1.4 4,515,215 + -2.7 4,515,215 + -0.5 4,515,215 + +0.0 4,515,215 + -0.9 4,515,215 + +0.4 4,515,215 + +0.8 4,515,217 + -0.9 4,515,217 + -0.2 4,515,217 + -0.5 4,515,458 + AO353_20585 0.15 +0.1 4,515,491 + AO353_20585 0.21 -1.1 4,515,492 - AO353_20585 0.22 +0.2 4,515,507 + AO353_20585 0.24 -1.4 4,515,507 + AO353_20585 0.24 +0.1 4,515,507 + AO353_20585 0.24 -0.2 4,515,507 + AO353_20585 0.24 -0.2 4,515,508 - +1.4 4,515,642 + AO353_20585 0.49 +1.3 4,515,642 + AO353_20585 0.49 -1.5
Or see this region's nucleotide sequence