Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_20200

Experiment: Spectinomycin 0.2 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.2 mg/ml
4,438,054	+		AO353_20185	0.88	-2.4
4,438,054	+		AO353_20185	0.88	-0.8
4,438,287	+				+0.6
4,438,287	+				-0.1
4,438,321	+				+0.1
4,438,343	+				+1.1
4,438,343	+				-0.1
4,438,343	+				-2.1
4,438,343	+				-1.4
4,438,346	+				-0.4
4,438,346	+				-2.1
4,438,346	+				+1.0
4,438,346	+				-0.1
4,438,346	+				+0.1
4,438,346	+				+1.4
4,438,346	+				-0.5
4,438,346	+				+1.0
4,438,350	+				+1.4
4,438,350	+				+1.2
4,438,350	+				+1.4
4,438,350	+				-1.6
4,438,382	+				+1.7
4,438,382	+				-1.4
4,438,394	+				-1.1
4,438,408	+				-2.0
4,438,408	+				-0.6
4,438,408	+				+0.7
4,438,408	+				-0.1
4,438,408	+				-1.7
4,438,418	+				+0.8
4,438,473	+				-0.6
4,438,479	+				-0.9
4,438,489	+				-1.4
4,438,763	+				+0.6
4,438,763	+				-0.9
4,438,933	+				-1.4
4,439,108	-				-2.3
4,439,108	-				+0.0
4,439,116	+				-0.1
4,439,116	+				-1.7
4,439,429	+		AO353_20200	0.23	-1.9
4,439,429	+		AO353_20200	0.23	-1.1
4,439,633	+		AO353_20200	0.34	-2.6
4,439,633	+		AO353_20200	0.34	+1.4
4,439,634	-		AO353_20200	0.34	-0.9
4,439,636	+		AO353_20200	0.34	-0.4
4,439,637	-		AO353_20200	0.34	+0.2
4,439,979	-		AO353_20200	0.53	+0.4
4,440,005	+		AO353_20200	0.54	-2.0
4,440,005	+		AO353_20200	0.54	-1.6
4,440,005	+		AO353_20200	0.54	-1.6
4,440,852	-				-1.1
4,440,873	+				+0.9
4,440,980	-				-2.3
4,441,035	+				-2.0
4,441,036	-				+1.2
4,441,050	+				-0.3
4,441,050	+				-1.4
4,441,061	-				-1.6
4,441,061	-				-1.8
4,441,097	+				-0.4
4,441,097	+				+0.1
4,441,097	+				-0.7
4,441,098	-				+0.4
4,441,098	-				+1.4
4,441,172	+				-1.1
4,441,194	+				-0.4
4,441,194	+				-0.3
4,441,194	+				+2.0
4,441,194	+				-0.0
4,441,194	+				-0.9
4,441,194	+				-1.6
4,441,195	-				+0.3
4,441,195	-				-1.6
4,441,195	-				+2.0
4,441,195	-				+1.2
4,441,195	-				+0.1
4,441,195	-				+0.6
4,441,195	-				-0.8
4,441,213	+				-0.4
4,441,213	+				-1.1
4,441,213	+				+0.6
4,441,213	+				-0.7
4,441,213	+				+0.2
4,441,213	+				-2.1
4,441,213	+				-1.2
4,441,213	+				+0.4
4,441,213	+				+1.2
4,441,213	+				-2.4
4,441,213	+				-0.3
4,441,213	+				-0.1
4,441,213	+				+0.9
4,441,214	-				-1.9
4,441,214	-				+1.1
4,441,214	-				+0.5
4,441,214	-				+0.6
4,441,214	-				-0.8
4,441,214	-				-1.7
4,441,214	-				-0.4
4,441,214	-				+0.1
4,441,214	-				+0.8
4,441,258	+				-1.1
4,441,259	-		AO353_20205	0.15	-2.0
4,441,259	-		AO353_20205	0.15	-0.7
4,441,259	-		AO353_20205	0.15	+0.9
4,441,259	-		AO353_20205	0.15	+1.2
4,441,259	-		AO353_20205	0.15	+0.9
4,441,259	-		AO353_20205	0.15	+1.4
4,441,318	+		AO353_20205	0.21	-0.9
4,441,318	+		AO353_20205	0.21	+0.3
4,441,319	-		AO353_20205	0.21	-1.0
4,441,319	-		AO353_20205	0.21	-1.1
4,441,319	-		AO353_20205	0.21	+0.3
4,441,319	-		AO353_20205	0.21	+0.6
4,441,405	-		AO353_20205	0.31	-1.1
4,441,489	-		AO353_20205	0.41	-1.7
4,441,603	+		AO353_20205	0.54	-0.4
4,441,603	+		AO353_20205	0.54	-2.4
4,441,604	-		AO353_20205	0.54	-0.4
4,441,665	+		AO353_20205	0.61	-1.1
4,441,838	+		AO353_20205	0.80	+0.9