Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_20170 and AO353_20175 are separated by 92 nucleotides AO353_20175 and AO353_20180 are separated by 106 nucleotides
AO353_20170: AO353_20170 - trigger factor, at 4,432,316 to 4,433,626
_20170
AO353_20175: AO353_20175 - ATP-dependent Clp protease proteolytic subunit, at 4,433,719 to 4,434,360
_20175
AO353_20180: AO353_20180 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 4,434,467 to 4,435,750
_20180
Position (kb)
4433
4434
4435 Strain fitness (log2 ratio)
-2
-1
0
1 at 4432.976 kb on + strand, within AO353_20170 at 4432.977 kb on - strand, within AO353_20170 at 4432.977 kb on - strand, within AO353_20170 at 4432.977 kb on - strand, within AO353_20170 at 4432.977 kb on - strand, within AO353_20170 at 4433.086 kb on + strand, within AO353_20170 at 4433.185 kb on - strand, within AO353_20170 at 4433.185 kb on - strand, within AO353_20170 at 4433.384 kb on + strand, within AO353_20170 at 4433.477 kb on + strand, within AO353_20170 at 4433.477 kb on + strand, within AO353_20170 at 4433.477 kb on + strand, within AO353_20170 at 4433.477 kb on + strand, within AO353_20170 at 4433.477 kb on + strand, within AO353_20170 at 4433.582 kb on + strand at 4433.582 kb on + strand at 4433.654 kb on + strand at 4433.654 kb on + strand at 4433.654 kb on + strand at 4433.655 kb on - strand at 4433.655 kb on - strand at 4433.693 kb on + strand at 4433.693 kb on + strand at 4433.693 kb on + strand at 4433.693 kb on + strand at 4433.697 kb on + strand at 4433.697 kb on + strand at 4433.697 kb on + strand at 4433.697 kb on + strand at 4433.697 kb on + strand at 4433.698 kb on - strand at 4433.698 kb on - strand at 4433.698 kb on - strand at 4433.734 kb on + strand at 4433.735 kb on - strand at 4433.735 kb on - strand at 4433.735 kb on - strand at 4433.819 kb on - strand, within AO353_20175 at 4433.827 kb on + strand, within AO353_20175 at 4433.881 kb on + strand, within AO353_20175 at 4433.882 kb on - strand, within AO353_20175 at 4433.953 kb on + strand, within AO353_20175 at 4434.036 kb on + strand, within AO353_20175 at 4434.037 kb on - strand, within AO353_20175 at 4434.061 kb on - strand, within AO353_20175 at 4434.345 kb on + strand at 4434.346 kb on - strand at 4434.346 kb on - strand at 4434.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 4,432,976 + AO353_20170 0.50 +1.0 4,432,977 - AO353_20170 0.50 -1.3 4,432,977 - AO353_20170 0.50 +0.0 4,432,977 - AO353_20170 0.50 -1.1 4,432,977 - AO353_20170 0.50 +1.1 4,433,086 + AO353_20170 0.59 +0.9 4,433,185 - AO353_20170 0.66 -0.6 4,433,185 - AO353_20170 0.66 +0.4 4,433,384 + AO353_20170 0.81 -0.6 4,433,477 + AO353_20170 0.89 +0.2 4,433,477 + AO353_20170 0.89 -0.4 4,433,477 + AO353_20170 0.89 -0.9 4,433,477 + AO353_20170 0.89 +0.0 4,433,477 + AO353_20170 0.89 +0.7 4,433,582 + +1.2 4,433,582 + -0.4 4,433,654 + +0.4 4,433,654 + -0.4 4,433,654 + -1.7 4,433,655 - -0.5 4,433,655 - +0.4 4,433,693 + +0.2 4,433,693 + -0.1 4,433,693 + +0.4 4,433,693 + -1.7 4,433,697 + +0.4 4,433,697 + -0.1 4,433,697 + -1.3 4,433,697 + -0.7 4,433,697 + +0.4 4,433,698 - -1.6 4,433,698 - -0.1 4,433,698 - +0.3 4,433,734 + -1.4 4,433,735 - -0.9 4,433,735 - -1.4 4,433,735 - -1.4 4,433,819 - AO353_20175 0.16 -2.3 4,433,827 + AO353_20175 0.17 -1.1 4,433,881 + AO353_20175 0.25 -1.7 4,433,882 - AO353_20175 0.25 +0.7 4,433,953 + AO353_20175 0.36 -1.1 4,434,036 + AO353_20175 0.49 +1.0 4,434,037 - AO353_20175 0.50 -1.0 4,434,061 - AO353_20175 0.53 -0.6 4,434,345 + +1.4 4,434,346 - +0.4 4,434,346 - -1.0 4,434,346 - -1.2
Or see this region's nucleotide sequence