Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_19350 and AO353_19355 are separated by 75 nucleotides AO353_19355 and AO353_19360 overlap by 25 nucleotides AO353_19360 and AO353_19365 overlap by 1 nucleotides
AO353_19350: AO353_19350 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 4,241,035 to 4,241,847
_19350
AO353_19355: AO353_19355 - dihydroneopterin aldolase, at 4,241,923 to 4,242,690
_19355
AO353_19360: AO353_19360 - peroxiredoxin, at 4,242,666 to 4,243,532
_19360
AO353_19365: AO353_19365 - thiol:disulfide interchange protein, at 4,243,532 to 4,245,268
_19365
Position (kb) 4242
4243
4244 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4241.752 kb on + strand, within AO353_19350 at 4241.845 kb on + strand at 4241.845 kb on + strand at 4241.846 kb on - strand at 4241.846 kb on - strand at 4241.846 kb on - strand at 4241.902 kb on + strand at 4241.902 kb on + strand at 4241.903 kb on - strand at 4241.925 kb on - strand at 4241.948 kb on - strand at 4241.948 kb on - strand at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.005 kb on + strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.006 kb on - strand, within AO353_19355 at 4242.054 kb on + strand, within AO353_19355 at 4242.054 kb on + strand, within AO353_19355 at 4242.055 kb on - strand, within AO353_19355 at 4242.055 kb on - strand, within AO353_19355 at 4242.055 kb on - strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.279 kb on + strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.280 kb on - strand, within AO353_19355 at 4242.314 kb on + strand, within AO353_19355 at 4242.314 kb on + strand, within AO353_19355 at 4242.315 kb on - strand, within AO353_19355 at 4242.315 kb on - strand, within AO353_19355 at 4242.315 kb on - strand, within AO353_19355 at 4242.401 kb on + strand, within AO353_19355 at 4242.401 kb on + strand, within AO353_19355 at 4242.453 kb on - strand, within AO353_19355 at 4242.494 kb on + strand, within AO353_19355 at 4242.495 kb on - strand, within AO353_19355 at 4242.495 kb on - strand, within AO353_19355 at 4242.495 kb on - strand, within AO353_19355 at 4242.518 kb on + strand, within AO353_19355 at 4242.519 kb on - strand, within AO353_19355 at 4242.817 kb on + strand, within AO353_19360 at 4242.827 kb on + strand, within AO353_19360 at 4243.334 kb on - strand, within AO353_19360 at 4243.345 kb on + strand, within AO353_19360 at 4243.346 kb on - strand, within AO353_19360 at 4243.346 kb on - strand, within AO353_19360 at 4243.748 kb on + strand, within AO353_19365 at 4243.749 kb on - strand, within AO353_19365 at 4243.810 kb on - strand, within AO353_19365 at 4243.810 kb on - strand, within AO353_19365 at 4243.810 kb on - strand, within AO353_19365 at 4243.810 kb on - strand, within AO353_19365 at 4243.810 kb on - strand, within AO353_19365 at 4243.810 kb on - strand, within AO353_19365 at 4243.813 kb on - strand, within AO353_19365 at 4243.813 kb on - strand, within AO353_19365 at 4243.813 kb on - strand, within AO353_19365 at 4243.813 kb on - strand, within AO353_19365 at 4243.813 kb on - strand, within AO353_19365 at 4243.813 kb on - strand, within AO353_19365 at 4243.813 kb on - strand, within AO353_19365 at 4243.813 kb on - strand, within AO353_19365 at 4243.945 kb on - strand, within AO353_19365 at 4243.945 kb on - strand, within AO353_19365 at 4243.945 kb on - strand, within AO353_19365 at 4244.089 kb on - strand, within AO353_19365 at 4244.118 kb on - strand, within AO353_19365 at 4244.118 kb on - strand, within AO353_19365 at 4244.341 kb on - strand, within AO353_19365
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 4,241,752 + AO353_19350 0.88 -1.5 4,241,845 + +1.5 4,241,845 + -0.6 4,241,846 - -0.0 4,241,846 - +0.8 4,241,846 - +0.2 4,241,902 + -1.0 4,241,902 + -0.0 4,241,903 - -1.0 4,241,925 - +0.1 4,241,948 - +0.1 4,241,948 - +1.7 4,242,005 + AO353_19355 0.11 +0.5 4,242,005 + AO353_19355 0.11 +0.9 4,242,005 + AO353_19355 0.11 +0.3 4,242,005 + AO353_19355 0.11 -0.6 4,242,005 + AO353_19355 0.11 -0.9 4,242,005 + AO353_19355 0.11 +0.9 4,242,005 + AO353_19355 0.11 +0.2 4,242,005 + AO353_19355 0.11 -0.3 4,242,005 + AO353_19355 0.11 +0.2 4,242,005 + AO353_19355 0.11 +1.4 4,242,005 + AO353_19355 0.11 +1.1 4,242,006 - AO353_19355 0.11 -0.3 4,242,006 - AO353_19355 0.11 +0.4 4,242,006 - AO353_19355 0.11 -1.3 4,242,006 - AO353_19355 0.11 +1.1 4,242,006 - AO353_19355 0.11 -1.9 4,242,006 - AO353_19355 0.11 -0.6 4,242,006 - AO353_19355 0.11 -1.4 4,242,006 - AO353_19355 0.11 -0.5 4,242,006 - AO353_19355 0.11 +0.8 4,242,054 + AO353_19355 0.17 +0.9 4,242,054 + AO353_19355 0.17 -0.3 4,242,055 - AO353_19355 0.17 +0.4 4,242,055 - AO353_19355 0.17 +1.0 4,242,055 - AO353_19355 0.17 -0.6 4,242,279 + AO353_19355 0.46 -0.2 4,242,279 + AO353_19355 0.46 +2.3 4,242,279 + AO353_19355 0.46 +0.4 4,242,279 + AO353_19355 0.46 -0.0 4,242,279 + AO353_19355 0.46 -0.4 4,242,279 + AO353_19355 0.46 +0.6 4,242,279 + AO353_19355 0.46 -1.9 4,242,280 - AO353_19355 0.46 -0.5 4,242,280 - AO353_19355 0.46 -0.2 4,242,280 - AO353_19355 0.46 +0.4 4,242,280 - AO353_19355 0.46 -2.0 4,242,280 - AO353_19355 0.46 -0.3 4,242,280 - AO353_19355 0.46 -1.5 4,242,280 - AO353_19355 0.46 +2.0 4,242,280 - AO353_19355 0.46 +1.2 4,242,280 - AO353_19355 0.46 -1.0 4,242,280 - AO353_19355 0.46 -1.0 4,242,280 - AO353_19355 0.46 +1.2 4,242,280 - AO353_19355 0.46 -1.0 4,242,280 - AO353_19355 0.46 -0.8 4,242,280 - AO353_19355 0.46 -0.6 4,242,280 - AO353_19355 0.46 -1.3 4,242,280 - AO353_19355 0.46 +0.1 4,242,314 + AO353_19355 0.51 +0.3 4,242,314 + AO353_19355 0.51 -0.0 4,242,315 - AO353_19355 0.51 -0.6 4,242,315 - AO353_19355 0.51 -0.7 4,242,315 - AO353_19355 0.51 +0.5 4,242,401 + AO353_19355 0.62 +0.4 4,242,401 + AO353_19355 0.62 +0.1 4,242,453 - AO353_19355 0.69 -0.4 4,242,494 + AO353_19355 0.74 +1.0 4,242,495 - AO353_19355 0.74 -1.9 4,242,495 - AO353_19355 0.74 +0.2 4,242,495 - AO353_19355 0.74 -0.9 4,242,518 + AO353_19355 0.77 +0.2 4,242,519 - AO353_19355 0.78 -1.3 4,242,817 + AO353_19360 0.17 -1.5 4,242,827 + AO353_19360 0.19 -2.3 4,243,334 - AO353_19360 0.77 -1.0 4,243,345 + AO353_19360 0.78 +0.3 4,243,346 - AO353_19360 0.78 -1.6 4,243,346 - AO353_19360 0.78 +1.5 4,243,748 + AO353_19365 0.12 +0.9 4,243,749 - AO353_19365 0.12 +1.9 4,243,810 - AO353_19365 0.16 -0.1 4,243,810 - AO353_19365 0.16 +0.4 4,243,810 - AO353_19365 0.16 +2.0 4,243,810 - AO353_19365 0.16 +0.7 4,243,810 - AO353_19365 0.16 +0.7 4,243,810 - AO353_19365 0.16 -0.5 4,243,813 - AO353_19365 0.16 -1.3 4,243,813 - AO353_19365 0.16 +0.7 4,243,813 - AO353_19365 0.16 +1.3 4,243,813 - AO353_19365 0.16 -1.1 4,243,813 - AO353_19365 0.16 +0.3 4,243,813 - AO353_19365 0.16 +0.8 4,243,813 - AO353_19365 0.16 +0.8 4,243,813 - AO353_19365 0.16 -0.9 4,243,945 - AO353_19365 0.24 +1.5 4,243,945 - AO353_19365 0.24 -0.1 4,243,945 - AO353_19365 0.24 +1.9 4,244,089 - AO353_19365 0.32 -0.5 4,244,118 - AO353_19365 0.34 +1.2 4,244,118 - AO353_19365 0.34 -1.1 4,244,341 - AO353_19365 0.47 -0.3
Or see this region's nucleotide sequence